With the methods of light microscopic histochemistry and electron-microscopic cytochemistry, we studied the changes of lactate dehydrogenase (LDH) and succinic dehydrogenase (SDH) in the anoxic myocardium of the mice; to which normal saline (INSAM group) or 10％ aqueous extract of Ginsengradix rubra (IGRAM group) was injected before the anoxic experiment. The results were as follows:Ⅰ. The Light Microscopic Study:1. In the myocardial sections of the INSAM group, the reaction of SDH of myofibrillae are variegated and the fibrillae vary in thickness. Granular mitochondria are clearly stained and arranged in alignment in the intermyofibrillar spaces. The intercalated discs fail to be stained and their spaces widen. In the IGRAM group, the reactions of SDH are similar to that of the normal control. The enzyme reaction product in the myofibrillae appear as homogeneous diffuse blue precipitate of formazan, the cross striations of myofibrillae are clear and strings of mitochondria are visible.2. The reactions of LDH in the INSAM group reveals that myofibrillar staining is also lost in a length of myofibrilla, strings of mitochondria are clearly stained. The reaction in the IGRAM group is similar to that of the normal control. In the myofibrillae there is a homogenous continuous violet precipitate of formazan.Ⅱ. The Electron-Microscopic Study:1. The reaction of SDH in the INSAM group shows that the enzyme activity in the mitochondrial inner membrane and the cristae is stronger than that of the normal control. The increased activity is represented by the increase of electronic density or darkening and thickening of the membranes. However, the activity in the IGRAM group is simillar to that of the normal control but weaker in reaction.2. The reaction of LDH in the INSAM group reveals that the enzyme activity exhibited by membrane darkening is obviously stronger than that of the normal control but in the IGRAM group it shows clearly a weaker reaction.The results mentioned above suggest that Ginsengradix rubra might alleviate the metabolic disturbance caused by myocardial anoxia.

The epithelial cells of human prostatic hyperplasia had been studied cytochemically for 5'-nucleotidase and thiamine pyrophosphatase activity with light and electron microscopy. The 5'-nucleotidase (5'-Nase) activities were present in the epithelial cells at lysosomes, Golgi vacuoles, vesicles, secretory vacuoles and free surface of plasmic membrane, the secretion material within the acinar lumina of the prostate gland also showed 5'-nucleotidase reaction product. The activity of 5'-nucleotidase has not markedly changed by the actinomyces globispores treatment, but slightly decreased after estrogen treatment. It was no enzyme reaction after treated by testectomy.Activities of thiamine pyrophosphatase (TPPase) were restricted to the Golgi area, and there were heavy deposits of reaction products in concave face of Golgi saccule. The activities of TPPase were not markedly changed by estrogen and actinomyces globispores treatments but none of enzyme activities could be seen after testectomy.

The data on nosocomial infection of 4060 patients who were stayed in intensive care unit (ICU) more than 48 h from January 1997 to August 2008 were reviewed.From Jan 2004 the target monitoring of hospital-acquired infection was implemented in ICU.The nosocomial infection rates before and after target monitoring were 35.25% (871/2471) and 30.77% (489/1589) respectively (P ＜ 0.01);however there was no significant difference in case infection rate before and after target monitor were practiced (47.67% vs.45.25%,χ~2 = 2.2836,P ＞0.05).The incidence of Acinetobacter infection was increased after target monitoring.Targeted monitoring can decrease the nosocomial infection rate in ICU,and also reduce the cost of medical care.

By means of pharmacohistochemical regimen method and image analysis system,the age-related changes of acetylcholinesterase activity of the substantia nigra neu-rons and the number and size(section area)of the AChE positive neurons in ratswere investigated quantitatively.The results showed that the number of AChE pos-itive neurons in the substantia nigra of the old rats decreased markedly,the rateand quantity of AChE synthesis in the perikaryon remarkable declined.The size(section area)of the AChE positive neurons also reduced with aging.These changeswith aging have never been studied in human or usual experimental animals,so thatsome new parameters were provided for the research field in experimental geronto-logy.In human and animal,the degeneration of the neurons in substantia nigra cau-sed by aging would disturb the balance between dopaminergic and acetylcholinergicsystem and hence interfere with the normal coordination of movement.

Objective To investigate the effect of curcumol on apoptosis of human gastric carcinoma cell line BGC823 and the molecular nechanisms.Methods BGC823 cells were cultured and treated with different curcumol concentration (12.5,25,50 and 100 mg/L) for 24 h and 48 h,and the growth inhibition were tested by thiazolyl blue terazolium bromide (MTF) assay.Flow cytometry (FCM) were used to measure the cell apoptosis rate and cell cycle of BGC823 cells.Caspase-3 activity was assessed by colorimetric assay.Cells treated with 100 mg/L curcunol for 48 h were collected and subjected to RTPCR and Western blot assays for the expression of Caspase-3,Bcl-2,Bax and Survivin.Results There was a time-and dose-dependent inhibition of cell proliferation of BGC823 cells by curcumol.Tbe cells in G0/G1 phase increased,and in S phase decreased on exposure to curcumol for 24 h.FCM analysis also indicated that the apoptosis rate of BGC823 cells increased in dose-dependent manner (P ＜ 0.05).Curcumol increased the activity of Caspase-3 dose-dependently (P ＜ 0.05).RT-PCR and Western blot indicated that curcumol decreased Bcl-2 and Survivin expression as well as increased Caspase-3 and Bax expression (P ＜ 0.05).Conclusions Curcumol inhibits BGC823 cell growth,arresting cells in G0/G1 phase and inducing cell apoptosis.The mechanism may be related with increasing the activity of Caspase-3,down-regulating the expression of Bcl-2 and Survivin,and up-regulating the expression of Caspase-3 and Bax.

Objective To investigate the expression of nuclear factor erythroid-2 related factor 2 (Nrf2) gene in chronic myeloid leukemia (CML) and explore its relationship with clinical characteristics and thioredoxin reductase (TrxR).Methods The expressions of Nrf2 and TrxR genes in bone marrow cells and K562 cells were analyzed in 30 bone marrow preparations of CML patients in different phases,including 20 in chronic phase,3 in accelerated phase,7 in blastic phase by real-time quantitative polymerase chain reaction.Ten health subjects were served as normal controls.Results The relative quantitation expression of Nrf2 and TrxR mRNA were 5.601±1.069 and 9.017±2.398 in chronic phase,1.698±0.349 and 5.590±1.015 in accelerated phase,1.252±0.807 and 5.050±1.469 in blastic phase,1.377± 0.344 and 1.867±0.977 in normal controls.The expressions of both Nrf2 and TrxR mRNA in CML had significant differences from those of the normal controls (x2 =14.680,P =0.002,x2 =8.271,P =0.041).The expression of Nrf2 mRNA in accelerated phase,blastic phase group showed no significant difference (Z =0.011,P =0.496),but lower than that in chronic phase group (Z =2.155,P =0.016,Z =2.534,P =0.006).The difference between the first visit and post-treated group was significant (Z =2.015,P =0.022).The expression in K562 cells and normal controls had significant difference (Z =1.898,P =0.029).In CML patients,the expression of Nrf2 was positively correlated with that of TrxR (r =0.738,P =0.037).Conclusion The expression of Nrf2 gene is higher in the first visit group of CML than that in the other groups,and is decreased after therapy,which may be the molecular marker predicting the progress of CML.Nrf2 mRNA expression level is correlated with TrxR.

OBJECTIVE Through nosocomial infection targeted surveillance in intensive care unit(ICU),to evaluate the characteristics of nosocomical infection and their its correlation with the results of environmental monitoring at the same period.METHODS Nosocomial infection monitoring was explored on patients who were in ICU more than 48 h from Jan 1997 to Jun 2008.Correlation analysis was conducted between the rate of nosocomial infection in ICU and the same period environmental monitoring results.Data were analyzed with the SPSS10.34 software.RESULTS There was significant difference(P0.05)in the rate of infection cases before and after target monitoring was practiced.The site of infection was mainly in respiratory tract,followed by the gastrointestinal tract and urinary tract;The full-time personnel in duty for nosocomial infection surveillance monitored the indoor air,object surface,medical personnel's hands in ICU,their qualified rate was 78.28%,90.94% and 95.83%,respectively.CONCLUSIONS There are many risk factors in patients in ICU,ICU should be looked as one of the important nosocomial infection monitoring department.The target monitoring of nosocomial infection is an effective method;the nosocomial infection rate in ICU and the environmental result at the same period don't show relevance,the environmental hygiene monitoring should be scientific and practical.

Objective To explore the activity of thioredoxin reductase (TrxR) in chronic myeloid leukemia cell line K562 and the anti-leukemia effect of BBSKE (a novel inhibitor of TrxR) in vitro. Methods The activity of TrxR on K562 cell lineage and fresh bone marrow cell from healthy adult was analyzed by insulin reduction assay. The inhibition of proliferation was measured by CCK-8 assay. The anti-leukemia effect of BBSKE was detected by laser scanning confocal microscope,agarose gel electrophoresis and flow cytometry with Annexin V -FITC/PI staining. Results TrxR activity of K562 cell lineage was significantly higher than that of normal bone marrow mononuclear cells. The apoptosis of K562 cells could be induced at concentrations of 10 μmol/L BBSKE after treated for 24 hours. The typical DNA ladder bans were observed by agarose gel electrophoresis. The apoptotic rates of K562 cells were (10.28±2.74) %. Application of 10 μmol/L BBSKE for 48 hours could also induce apoptosis of fresh bone marrow cell from chronic myeloid leukemia patients, and the apoptotic rates were (5.70±0.48) %. Conclusion TrxR activity in chronic myeloid leukemia cells was significantly higher than that of normal cells. BBSKE inhibits the TrxR activity and the proliferation of K562 by inducing apoptosis.It might be a potential medication for chronic myeloid leukemia.

OBJECTIVE: To investigate the gene expression profile by using gene chip technology and probe into the role of corresponding gene in the pathogenesis of nasal polyps by analysing the difference of the gene depression. METHOD: The total RNAs were respectively extracted from 6 pairs of inferior turbinates and nasal polyps, and then were reversely transcribed to cDNAs with incorporation of fluorescent dUTP as the hybridization probes. The mixed probes were then hybridized with the BiostarH-40 s gene chips, it was scanned by laser scanner and the acquired image was analyzed by software. RESULT: 1887 genes were differently expressed in gene profile of nasal polyps, among which 1099 were upregulated and 788 were down-regulated. Six genes were found in all gene chips, among which 4 genes were upregulated and 2 were down-regulated. The 6 genes encoded the protein of the transmembrane 4 superfamily, highly similar to GAMMA-interferon-inducible protein IP-30 precursor, highly similar to complement factor I precursor and insulin-like growth factor binding protein 3 (IGFBP3). CONCLUSION Detecting the differently expressed genes will provide clues and theoretical foundation for the pathogenesis of nasal polyps. The nasal polyps is a polygenic disease and the genes of GAMMA-interferon-inducible protein, insulin-like growth factor binding protein may play an important role in its pathogenesis.
Adult ; Gene Expression Profiling ; methods ; Humans ; Male ; Middle Aged ; Nasal Polyps ; genetics ; Oligonucleotide Array Sequence Analysis ; methods ; Young Adult

Objective To explore the work experiences in nursing homes among nurses and to provide the referenced basis for managers to understand the needs of nurses and develop humanistic nursing management measures. Methods Descriptive phenomenological methodology was adopted in this study,a semi-structured interview was conducted with 9 nurses working in nursing homes.Data were analyzed based on Colaizzi′s phenomenological research method. Results Four themes were extracted including:heavy work tasks;high psychological pressure;strong demand in training;emotional experience of work. Conclusions Through in-depth interviews with 9 nurses working in nursing homes,we suggest that in order to promote the professional development of geriatric nursing, managers should change and update the concept of geriatric nursing and attach importance on professional study of nurse, and take appropriate intervention measures to lighten nurses' work pressure and adjust their bad mood, so as to arouse the enthusiasm of nurses.Finally,we can achieve the goal of stabilizing the nursing personnel and improving the quality of care in the nursing homes.