Objective To establish a method for the determination of aristolochic acid A in Jiuwei-Qianghuo pill. Method The HPLC system consisted of the Phenomenex Luna C18(4.6 mm × 250 mm, 5μm)column, the mobile phase consisted of acetonitrile and 0.05% H3PO4, gradient elution flow rate was 1.0 ml/min, the column temperature was 35℃, the UV detector was set at 250 nm.Results The linear response range was 0.014-0.280μg/ml(r=0.999 9). The detection limit and quantitation limit of aristolochic acid A in Jiuwei-Qianghuo pill was 1.5 and 4.5 ng/ml. The average recovery of aristolochic acid A was 96.3%. Conclusions The method was high sensitivity, accurate, repeatable and high secificity. It can be used as an inspection method for safe use of Jiuwei-Qianghuo pill.

2.2 in serum, the particle size of HDL shifted towards smaller sizes, which indicates that reverse cholesterol transport might be weakened and HDL maturation might be abnormal.

AIM: To investigate apolipoprotein A-Ⅰ gene (Apo A-Ⅰ) polymorphism and its relationship with serum HDL subclasses in patients with hyperlipidemia (HL). METHODS: Apo A-Ⅰ genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 118 patients with hyperlipidemia and 109 healthy subjects were determined by two-dimensional gel electrophoresis conjunction with immunodetection method. RESULTS: Both in HL group and the control group, G/G and C/C genotypes were the most frequent at -78 bp and +83 bp of Apo A-Ⅰ gene, respectively. The frequency of rare A allele at -78 bp in HL group was significantly higher than that in control group. In HL group, subjects with G/A mutation had higher serum levels of TG, Apo C-Ⅲ, pre ?_1-HDL and HDL_ 3a , and lower levels of HDL_ 2a and HDL_ 2b compared to the subjects with G/G genotype. CONCLUSION: The G/A transition in the -78 bp position of the Apo A-Ⅰ gene promoter in patients with hyperlipidemia is associated with HDL subclasses. There is a general shift toward smaller sized HDL, which, in turn, indicates that HDL maturation might be abnormal.