Objective: To study the nursing of patients with morbid obesity treated with laparoscopic vertical banded gastroplasty (LVBG). Methods: Before operation, obese degree, obesity-related conditions and mental states were examined routinely. Monitoring of respiratory tract, observing operative complications and instructing of diets were done after operation. Results: Among 6 patients, 5 were at the third degree of obese, one was at second. In obesity-related conditions, 4 patients had hypertension and acantha derma, 1 had arthritis, and all had respiratory sleeping syndrome. The operations were all successful. The food amount food and body weight both decreased significantly 1 month after operation. The common operative complications were mild bleeding (1 case), shoulder-back pain (1 case), nausea and vomiting (5 cases). Diet principle was high protein, low energy, liquid food was the first choice. Conclusion: Observing and preventing respiratory sleeping syndrome are the main points of postoperative cares. Instructing patients to establish correct diet habit is the key to reach the best efficacy of LVBG.

Objective To evaluate the effect of limb remote ischaemic preconditioning on pul-monary function in patients undergoing cardiac valve replacement surgery with cardiopulmonary by-pass.Methods Seventy patients,32 males and 38 females,aged 18-70 years,weighing 45-90 kg, ASA physical status Ⅱ or Ⅲ,scheduled for elective cardiac valve replacement surgery with cardiopul-monary bypass,were divided into 2 groups using a random number table,35 in each group.Patients in group R received three cycles of right upper-limb 5 min ischemia (blood-pressure cuff inflation to≥ 200 mm Hg)and 5 min reperfusion (blood-pressure cuff deflation to 0 mm Hg)at 10 min after in-tubation.In group C,the cuff was placed around the arm but not inflated.At 10 min after intubation (T0),at 1 h after aortic declamping (T1)and at 6 h (T2),12 h (T3),24 h (T4)after surgery,arte-rial blood was sampled to conduct gas analysis,PaO2/FiO2ratio and alveolar-arterial oxygen gradient (A-aDO2)were calculated,and the dynamic lung compliance (Cd)and static lung compliance (Cs) were also recorded.The occurrence of pulmonary adverse events was recorded until discharge. Results Compared with T0,PaO2/FiO2was decreased in the two groups at T1-T4,A-aDO2was de-creased at T2-T4,Cs and Cd were increased in group C at T3,and were increased in group R at T2, T3(P<0.05).Compared with group C,the Cs and Cd at T2,T3were increased in group R.There were no significant differences between the two groups in the PaO2/FiO2,A-aDO2at T0-T4.The oc-currence of the pulmonary adverse events was decreased significantly in group R than in group C (P<0.05).The occurrence of pulmonary adverse events was declined significantly in group R than in group C (P<0.05).Conclusion Limb remote ischemic preconditioning can improve the lung compli-ance and reduce the occurrence of the pulmonary adverse events in patients undergoing cardiac valve replacement surgery.

Objective To evaluate the continuation of care for colorectal cancer patients with stoma mental state and a-bility of self-care. Methods Fifty-six cases Miles patients in the observation group and 30 patients Miles patients in the control group were treated with extended care and routine care respectively, the depression, anxiety, self-care, quality of life and the incidence of complications of patients in two groups were compared after discharging for three months. Results After discharging for three months, the SDS scores and depression levels in the observation group were significantly lower than the control group(P<0.05), SAS score the of observation group were significantly lower than the control group(P<0.05), moderate and mild depression were significantly lower in the control group (P <0.05), ESCA to-tal score, skill, responsibility, self-concept and health knowledge scores of the observation group were significantly higher (P <0.05), SF-36 physical function scale, social function, emotional function, mental health, energy and overall health scores of the observation group were significantly higher (P <0.05), stoma complications in the observation group was significantly lower than the control group(P<0.05). Conclusion Extended care effectively improve colorectal cancer ostomy patients mental state, improve their self-care ability stoma, thereby improving the quality of life and reduce the incidence of stoma complications.

Objective To explore the antiarrhythmic mechanism of ampelopsin through electrophysiological study in rats.Methods The in vivo experimental groups were as follows:control group,low-dose,middle-dose and high-dose group.Arrhythmia in rats was induced by aconitine injection,and then the antiarrhythmic effects of ampelopsin were studied.Cardiomyocytes were isolated from rats therafter.The whole-cell patch-clamp technique was used to record action potential duration (APD),sodium currents (INa),calcium current (ICa),transient outward potassium currents (Ito) and inward rectifier potassium currents (IK1) in cardiomyocytes.Results In vivo experiments showed that the incidence of aconitineinduced experimental arrhythmias in low,middle and high-dose ampelopsin group was significandy lower than that in control group (n =5 each group,all P ＜ 0.05).In vitro whole-cell patch clamp experiments showed that action potential duration in low,middle and high-dose groups was significantly shorter than that in control group,and amplitude of action potential was also significantly lower in low,middle and high-dose ampelopsin groups than in control group (134.1 ±6.9),(120.1 ±7.4),(113.2 ±9.0),and (101.8 ±5.1) mV for control,low,middle and high-dose group (n =9 each group,all P ＜ 0.05).Further research revealed that sodium currents in cardiomyocytes were decreased by low,middle and high-dose ampelopsin from (-36.75 ±3.60) to (-31.03 ±2.61),(-26.63 ±3.72),and (-17.55 ±4.43) pA/pF (n =9each group,all P ＜ 0.05),but the activation voltage for peak potential was not affected by ampelopsin.Moreover,the inward rectifier potassium current was also higher in high-dose ampelopsin group than in control group (P ＜ 0.05).Calcium current and transient outward potassium current were similar among four groups.Conclusion Ampelopsin exerts anti-arrhythmic effects in this rat model,and the underlying electrophysiological mechanism is partly associated with the inhibition of INa and enhancement of IK1,and prolongation of APD.