Objective:To study rosiglitazone therapy in PCOS infertility patients with insulin resistance.Methods:Among 38 PCOS patients,15 insulin-resistance cases were identified via hyperinsulinemic euglycemic clamp technique(HGCT).Rosiglitazone(Avandia) were administratod at 4mg qd.HGCT,oral glucose tolerance test(OGTT),the levels of insulin,T,FSH, LH were tested before and after the treatment.The follicle growth were observed by transvaginal ultrasonography,while the follicle growth rates were compared.Results:After treatment with combined rosiglitazone with clomiphene citrate(CC) 50mg,qd,HGCT value increased (P

Objective:To investigate the indicative value of the serum leptin level in obesity and non-obesity PCOS patients treated with insulin-sensitizing agent rosiglitazone.Methods:73 PCOS patients were divided into obesity(43)and non-obesity group(30).The changes of the serum leptin level and the relevant indexes were observed before and after treament with rosiglitazone.Results:the leptin level of PCOS groups was higher than that in control and the level in obesity PCOS patients was higher than that in non-obesity PCOS patients both before and after treatment.After treatment,the leptin levels in both PCOS groups were all significantly decreased,greater decrease in obesity group.Correlation:analysis revealed that the leptin level had positive correlation with BMI,FINS and LH,but negative correlation with the HGCT.Conclusion serum leptin level has some indicative value in the assessment of insulin-sensitizing agent in treating PCOS.

BACKGROUND:At present, bone substitute filling is mainly used for bone defect repair. In order to understand the effect on bone defect repair, it is necessary to look into the microstructure changes of bone defects after bone substitute implantation. Synchrotron radiation-based micro-computed-tomography (SR-μCT) can be used to make high-resolution, high-resolution three-dimensional imaging without slicing and dyeing, and has high scientific and clinical value. OBJECTIVE:To explore the feasibility of SR-μCT in micron-level bone osseointegration examination in oral medicine. METHODS:This randomized controlled animal experiment was completed at the Fifth Central Hospital of Tianjin, Tianjin, China. A rabbit model of mandibular defect was made in 24 male New Zealand white rabbits. The model rats were randomly divided into four groups and received autologous bone, Bio-oss bone meal,β-tricalcium phosphate powder and no implantation (negative control group) in the defective area, respectively. Bone samples, including the defect area and the surrounding normal bone tissue, were taken at 2, 4, 8 weeks postoperatively for SR-μCT examination, fol owed by histopathological examination, in order to observe the repairing effects of different types of bone implant materials from different angles. The study protocol has been approved by the Ethics Committee of the Fifth Central Hespital of Tianjin in China. The study procedures were completed in accordance with the Guidance Suggestions for the Care and Use of Experimental Animals of China and the guidelines of the National Institutes of Health, USA. RESULTS AND CONCLUSION:In this study, SR-μCT could be used to observe the bone microstructure and osseointegration with no damage to samples to collect accurate quantitative data, including bone volume, number of bone trabeculae and bone mineral density. Therefore, SR-μCT can fully analyze the biocompatibility of bone implant material in vivo, give insight into the micron-level changes of different types of bone implant materials in the bone defect, thereby providing experimental evidence to improve bone defect healing.

Objective To evaluate the effect of the systematic education platform for patient with colostomy in improving self‐care ability .Methods A total of 198 colostomy patients from a Third‐Grade Class A Hospitals were randomly assigned to ei‐ther learn colostomy nursing knowledge by the systematic education platform or routine health education condition .The patients were investigated by a questionnaire about Exercise of Self‐Care Agency Scale ,ESCA before discharge ,1 month and 3 months after discharge .Results After the systematic education platform for 3 month ,the total score(120 .87 ± 10 .11) and each factor scores of ESCA scale[self‐protection skills(30 .51 ± 2 .57) ,self nursing responsibility(23 .95 ± 2 .64) ,self concept(26 .23 ± 1 .69) ,health knowledge level (39 .78 ± 3 .12 )] were all significantly improved after the systematic education platform ( P < 0 .05 ) . Conclusion The systematic education platform can improve ostomy patients′self‐care ability and quality of life .

Objective To investigate the role of subtype 1 autoantibody against angiotensin Ⅱ receptor in the pathogenesis of preeclampsia by detecting its expression in the peripheral blood of preeclampsia patients,Methods Thirty patients with preeclampsia were assigned to preeclampsia group. Twenty normal pregnant women at the late stage and twenty non-pregnant healthy women as controls were investigated. The level of type 1 antoantibody against angiotensin Ⅱ in the peripheral blood was detected by indirect SA-ELISA assay with the produced ATR-1 as the antigen. Results The level of subtype 1 antoantibody against angiotensin Ⅱ (65 ±4. 7) % in the peripheral blood of preeclampsia patients is significantly higher than that of normal late pregnant (26 ±2. 8)% and non-pregnant women(7.8 ±2. 2)% groups (t1 =24. 97 ;t2 =38.56;P ＜0. 01 for both) ;The angiotensin Ⅱ receptor subtype 1 autoantibody in the group of normal late pregnancy (26 ± 2. 8 )% was significantly higher than that of healthy non-pregnant women group ( 7. 8 ± 2. 2 ) % ( t = 4. 58, P ＜ 0. 05 ).Conclusion Compared with the normal pregnant women and the healthy non-pregant women, the autoantibody against AT1 receptor in sera of preeclamptic patients is elevated ata high frequency. These results suggest that overproduction of AT1-AA may play an important role during the development of preeclamptic patients. AT1-AA is a novel risk factor in pregnant women. Immune mechanisms may be involved in the process of pregnancy.

At present, the particularity and importance of ethical consciousness in the teaching of clinical obstetrics and Gynecology has become increasingly prominent while the education of medical students in the ethics and social responsibility is relatively weak. This study explored a series of practical methods under WEIGHT guide through the real cases, conducting clinical training and sides debate, com-bining multimedia and interview form, in order to strengthen the cultivation of medical students' moral quality and improve the students' comprehensive ability.

BACKGROUND:Absorbable suture with good biocompatibility and mechanical strength has been extensively used in the clinic. OBJECTIVE: To explorein vitro degradation of different absorbable sutures, to analyze the clinical superiority of absorbable sutures and the influential factors for itsin vitro degradation. METHODS: After retrieval of related literatures concerning degradation and application of absorbable suture material, we retrospectively analyzed relevant articles addressing influential factors for performance and degradation of absorbable suture materials, and comparison of clinical absorbable and nonabsorbable sutures, thereby providing theoretical evidence for clinical application. RESULTS AND CONCLUSION: Absorbable sutures can be completely degraded and absorbed by the body, with no adverse reaction and no scars after wound healing. Absorbable sutures overcome the shortcomings of nonabsorbable sutures that cannot break down in the body. The absorption of its degradation products in vivo has two pathways: one is the phagocytosis of giant cels and phagocytes in the body, and the other is that the degradation products enter the body fluids and blood. For example, lactic acid is absorbed and excretedvia the systemic circulation. There is a very complicated physiological environment in the human body, and various factors can affect material properties. In addition, the different kinds of materials have different degradation mannersin vivo. Therefore, it is necessary to design, synthesize and process biodegradable polymer materialswith special properties within a certain range to meet the clinical needs.

Objective To investigate the significance of EGF in the in vitro maturation, fertilization, and pre-implantation development of the immature oocytes. Methods The immature oocytes were isolated from the ovaries of the rats. The germinal vesicle (GV) oocytes were cultured in the EGF solutions at concentrations of 0(control), 0.5, 1, 2.5, 5 and 10 ng/ml Ham’s F10 culture media. The status of germinal vesicle break-down(GVBD) and the extrusion of the first polar body (PB1) were observed. The achieved MⅡoocytes were then inseminated in vitro; and the pronuclei (PN) and the pre-implantaon development were observed. The gradeⅠ-Ⅱ4-cell embryos were selected and implanted into the fallopian tube of the rats with the 1 day pseudogestation. Follow-up observations were made till the delivery. Results After GV oocytes were treated with EGF solutions at concentration of 1, 2.5, 5 and 10 ng/ml for 12, 16, 20, and 24 h, the GVBD in the GV cumulus-oocyte complexs(COC) have better outcomes than that of the control group(P

Objective To study the feasibility of regional network platform for wound care technical training.Methods Micro-letter pubic number and internet technologies were used to develop a regional network platform of wound care which has one center with multiple branches,training nurses how to take care of wound by the educational column.Results There were 383 nurses had browsed the web site and took part in the learning since regional network platform was working 3 months,931 nurses paid close at-tention to Micro-letter pubic number.Performance examination was improved obviously after training,the percent of pass was 17.8% before training and 80.7% after training,the difference was with statistical significance (χ2=303.629,P<0.01).Conclu-sion The regional network platform of wound care based on micro-letter and internet technologies has a promising application.

The difficult knowledge was analyzed in detail from such aspects as medical students' cognitive ability of the female pelvic anatomy structure,their learning of female reproductive endocrine physiology,their understanding of delivery mechanism and gynecological tumor clinical staging in gynecology and obstetrics.Through such strategies as the diversification of teaching to deepen students' cognition of female pelvic anatomy structure,presentation method to strength their understanding of the delivery mechanism and repeated memory to reinforce their learning of female reproductive endocrine physiology and smart memory to remember gynecologic oncology clinical staging,not only the students' learning ability was improved,but also the teaching quality was enhanced.