Objective To explore the effect of angiotensin-(1-7) on the angiotensin-II(Ang II) induced proliferation of cultured rat's glomerular mesangial cells(GMC). Methods Ang-(1-7) was used in the cultured rat's GMC treated by AngⅡ, the synthesis of DNA and protein in GMC was measured by incorporation of thymidine and leucine, respectively, and the proliferation of GMC was measured by crystal violet staining. Results Ang-(1-7) inhibited the synthesis of DNA and protein of cultured GMC treated by AngⅡ in a dose-dependent manner. Ang-(1-7) also reduced the number of GMC treated by AngⅡ.The effects of Ang-(1-7) could not be blocked by both -AngⅡ,a specific AngⅡAT1 receptor antagonist, and AngⅡAT_2 receptor antagonist PD123319. Conclusion Ang-(1-7) could inhibit the proliferation of cultured rat's GMC induced by AngⅡ. The effects of Ang-(1-7) were not mediated by AngⅡAT1 and AT2 receptors.

Objective: To explore the influence of angiotensin-(1-7) \[Ang-(1-7)\] and phorbol 12-myristate 13-acetate (PMA)on the proliferation and secretion of cultured rat glomerular mesangial cells (GMC) induced by angiotensinⅡ(AngⅡ). Methods: Ang-(1-7) and PMA was used in cultured rat glomerular mesangial cells induced by AngⅡ, synthesis of DNA and protein, change of cell number were observed for rat GMC proliferation. Secretion of PcⅢ and HA was measured by radioimmunoassay in culture medium of rat GMC. Results: Ang-(1-7) and PMA both inhibited the AngⅡinduced synthesis of DNA and protein, increase of cell number, and secretion of PcⅢ and HA in cultured rat GMC. Conclusion: Ang-(1-7) and PMA both can inhibit the AngⅡ induced proliferation and secretion of cultured rat GMC.