1.The Study of Inhibition of Malignant Phenotype of SAC-IIB2 Cell Strains by Antisense Oligodeoxynucleotide of c-fos
Kaiping YAN ; Lianhua YIN ; Xianshou KONG
Chinese Journal of Cancer Biotherapy 1994;0(01):-
It was found that protooncogene c-fos was over-expressing in SAC-IIB_2 cell strains. Targeting the code 2-7 sequence of c-fos cDNA of mice, we designed and synthesised eighteen mers antisense. sense and nonsense oligodeoxynucleotides(ODN). The results indicated that antisense c-fos ODN could induce SAC- IIB_2 maturative differentiation, inhibit its growth rate, decrease its ability of forming tumor in nude mice and expression of Fos protein, however, antisense ODN has no remarkable effect on cellular dynamics of SAC-IIB2. But sense and nonsense c-fos ODN have no similar effect on SAC-IIB_2 cell strains. The results demonstrated that antisense c-fos oligodeoxynucleotide has inhibition effects on malignant phenotype of mice SRS lymphoma cell.
2.The discussion about the application of trust financing in public hospitals under the new background
Min LUO ; Yaxin HUANG ; Lianhua KONG
Chinese Journal of Hospital Administration 2013;29(12):934-937
Trust financing is a new financial instrument on the market at present.Combined with the new health reform and macroeconomic background,the paper firstly summarized the basic theory of public hospitals'trust financing,discussed the feasibility of public hospitals'trust financing,then put forward some policy suggestions for how to perfect trust financing for public hospitals from improving external environment,increasing government support,optimizing of hospital risk mitigation mechanism and income security mechanism,regulating hospital project information disclosure,training professional talents on the basis of analysis of three models of public hospitals' trust financing.
3.Effects of anti-leukemia by antisense c-myc by retrovirus vector
Xinyong ZHAO ; Lianhua YIN ; Xianshou KONG ; Yiqun YANG
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To study the role of c-myc oncogene in L6565 leukemia oncogenesis and the effects of therapy by inhibition of its expression with antisense c-myc. METHODS: A recombinant retroviral vector containing antisense c-myc of the murine (pGNCas)was constructed and then transfected into PA317 cells by the method of calcium phosphate precipitation. L6565 clone cells were infected with retrovirus particles. Stable integretion of antisense c-myc was shown by PCR. The change of the malignance and phenotype of L6565as were detected by the examination of the growth, morphology, cells cycle, agar assay and expression of c-myc. RESULTS: The shape of most L6565as cells became spherical. The growth of L6565as was inhibited compared to control cells. The analysis of cells cycle: L6565as cells were arrest in G 0/G 1 phase, decreased in S phase. The ability of L6565as cells to form colony in soft agarose was significantly suppressed. c-myc in L6565as cells was lowly expressed. CONCLUSION: (1)c-myc plays a critical role in L6565 leukemia oncogenesis; (2)Inhibition of expression of c-myc makes partly reversion of malignant phenotype of L6565 murine leukemia clone cells.
4.Progress in Research of Electrigens in Microbial Fuel Cell
Ying LI ; Yongming SUN ; Xiaoying KONG ; Lianhua LI ; Zhenhong YUAN ; Xiushan YANG
Microbiology 2008;0(09):-
Electricigens play an important role in microbial fuel cell(MFC) . This review provides an introduction of different electricigens on theirs taxonomical group,biochemical,physiological and morphological characteristics. The ability of electricity production of electricigens and electron transfer mechanisms in microbial fuel cells are also concluded. The prospect of waste water treatment and bio-electricity production is underlined,it is point out in this review that the future research of microorganism for MFC should be focused on enrichment,adaptation,modification and optimization by multi-strains application to improve the performances of MFC.
5.A retrospective study of clinical and immunologic features of 67 cases of patients with scrub typhus
Yiqing ZHOU ; Lingyao XU ; Lianhua KONG ; Qian WANG ; Yaping HAN ; Jun LI ; Ke JIN
Chinese Journal of Endemiology 2015;34(10):713-716
Objective To study the clinical and immunologic features of 67 cases of patients with scrub typhus.Methods Epidemiological data,clinical manifestations,laboratory and image examination results and treatment of 67 cases of patients with scrub typhus hospitalized from January 2010 to December 2013 at the First Affiliated Hospital of Nanjing Medical University were retrospectively analyzed.Results Autumn-winter type scrub typhus was predominant in the 67 patents.The main clinical manifestations included hyperpyrexia (100%,67/ 67),eschar or ulcer (91.0%,61/67),rash (52.2%,35/67),lymphadenopathy (46.3%,31/67) and other non-specific symptoms.Laboratory test results:①80.6% (54/67) of the patients had normal or decreased white blood cell count,62.7% (42/67) of them with elevated lymphocytes (> 40%),thrombocytopenia was detected in 34.3% (23/67) of the patients,the lowest platelets count was 14 × 109/L.②14 of 17 cases (82.4%) had normal percentage of CD3+ T lymphocytes,CD8+ T lymphocytes increased in 16 of 17 cases (94.1%),while the percentage of CD4+ T lymphocytes (14 of 17,82.4%) and B lymphocytes (15 of 17,88.2%) decreased in most of the patients.③C reactive protein (CRP),ferritin and erythrocyte sedimentation rate (ESR) were elevated in 95.2% (59/62),93.8% (45/48) and 89.1% (49/55) of the patients,respectively.④Elevated alanine aminotransferase (77.6%,52/67) and aspartate aminotransferase (80.6%,54/67) were frequent findings.⑤94.6% (35/37) of the patients had increased adenosine deaminase.⑥ Antinuclear antibody was positive in 31.0% (13/42) patients.⑦Epstein-barr virus DNA was detected in 15.6% (7/ 45) cases,77.6% (38/49) was accompanied with chest radiographic abnormalities.Two cases had tuberculosis.All the patients recovered after therapy with doxycycline,levofloxacin or moxifloxacin.Conclusion Autumn-winter scrub typhus has typical clinical manifestations in the 67 patients,and it can cause patients' immune disorders.
6.The association between antigen-specific cytotoxic lymphocytes response and different clinical status in patients with hepatitis B
Yufeng WAN ; Yaping HAN ; Jun LI ; Lianhua KONG ; Shuang LI ; Li DONG ; Nian CHEN ; Yuan LIU ; Zuhu HUANG
Chinese Journal of Infectious Diseases 2009;27(5):287-291
Objective To analyze human leucocyte antigen (HLA)-A0201 restricted antigen-specific cytotoxic lymphocytes (CTL), and to investigate the difference of T cell response to specific antigen epitopes between patients with acute phase of acute hepatitis B and active phase of chronic hepatitis B. Methods Peripheral blood mononuclear cells (PBMC) from 5 patients with acute phase of acute hepatitis B and 6 patients with active phase of chronic hepatitis B were isolated. The numbers and functions of CD8+ T-lymphocyte epitope peptide specific CTL were detected using enzyme-linked immunosorbent spot (ELISPOT) assay, and the 3 peptides were from HBV polymerase region (Pol575-583), envelope region (Env348-357) and core region (Core18-27), respectively. The data were analyzed using t test. Results The spot formation cell counts (SFC) of Pol575-583, Env348-357 and Core18-27 stimulations in patients with acute phase of acute hepatitis B were 110±13, 165±17 and 185±20, respectively; and those in patients with active phase of chronic hepatitis B were 22±4, 23±5 and 30±5, respectively; the differences were all significant (t=10.9, 15.2 and 8.0, respectively, all P<0.05). The CTL responses to the three peptides in patients with acute phase of acute hepatitis B were Pol575-583
7.Viral load and cytokines in the pathogenesis of severe fever with thrombocytopenia syndrome
Yaping HAN ; Li DONG ; Lianhua KONG ; Lili ZHANG ; Ning LIU ; Nian CHEN ; Ke JIN ; Qian WANG ; Zuhu HUANG ; Jun LI
Chinese Journal of Infectious Diseases 2014;(9):538-544
Objective To observe the relationship of viral load,serum cytokines and tissue damage after severe fever with thrombocytopenia syndrome virus (SFTSV)infection,and to explore the impact of SFTSV levels on tissue injury and prognosis.Methods Twenty-four ambulatory and hospitalized patients who were infected with SFTSV were enrolled between May 2011 and July 2012 at Department of Infectious Disease, First Affiliated Hospital with Nanjiang Medical University. According to their prognosis,they were divided into cure and death group,while 32 healthy blood donators were also enrolled from center blood station in Nanjing as control.The serum SFTSV load was detected using fluorescence quantitative polymerase chain reaction (PCR).The serum T helper (Th)1/Th2/Th17 cytokines in patients with severe fever with thrombocytopenia syndrome (SFTS)were determined dynamically and quantitatively by flow cytometry.The relationships between viral load,cytokines and serum enzymes, white blood cell (WBC),platelet (PLT)counts were analyzed.Comparisons among groups were achieved by rank sum test and correlation analysis among serum cytokines,blood cell counts and tissue damage was done by Spearman correlation test.Results All of the 24 patients showed a positive reaction to SFTSV RNA.The SFTSV loads of 21 cured cases,those of 2 were > 7.0 lg copy/mL,and those of 3 death patients were 6.7 lg copy/mL,8.8 lg copy/mL and 9.8 lg copy/mL,respectively.Serum level of interleukin (IL)-6 (21 .76 pg/mL in day 5 and 7.12 pg/mL in day 7)and IL-10 (14.33 pg/mL in day 5 , 14.13 pg/mL in day 7 and 3.01 pg/mL in day 9)of cured patients were significantly higher than those of healthy controls (IL-6:2.82 pg/mL and IL-10:1 .56 pg/mL)(P <0.05 ).At day 7 and day 9,serum levels of IL-6 of death cases were 137.61 pg/mL and 1 450.83 pg/mL,respectively and serum levels of IL-10 were 50.26 pg/mL and 49.43 pg/mL,respectively.Both of the indicators in the death group were significantly higher than those of cure group (P <0.05 ).However,serum levels of IL-2 and IL-4 were significantly lower than those in healthy control group (P <0.05 ).In the cure group,WBC and PLT counts were lowest during the early course of the disease,and serum alamine aminotransferase (ALT), aspartate aminotransferase (AST ), lactic dehydrogenase (LDH ) and creatine kinase (CK ) were significantly higher than their upper limits of normal.The correlation analysis showed that serum IL-6, IL-10 levels were negatively correlated with PLT count (r=-0.390 and -0.608,respectively;both P <0.01),and positively correlated with SFTSV load (r=0.560 and 0.758,respectively),ALT (r=0.412 and 0.390,respectively),AST (r = 0.686 and 0.764,respectively),LDH (r = 0.633 and 0.677, respectively)and CK (r =0.527 and 0.636,respectively)(all P <0.01 ).Conclusions SFTSV load, IL-6,IL-10 and serum enzyme levels are closely related to the severity of the disease.The inflammatory and anti-inflammatory cytokine storm after SFTSV infection may be involved in the immune pathological injury in patients with SFTS.
8.Association of dieting and binge eating with non suicidal self-injury among secondary vocational school students
Chinese Journal of School Health 2024;45(12):1761-1765
Objective:
To explore the relationship between dieting and binge eating with non suicidal self-injury (NSSI) among secondary vocational school students, so as to provide a scientific basis for the development of effective interventions to prevent NSSI.
Methods:
From October to December 2023, 2 364 students were selected by stratified cluster random sampling method from nine secondary vocational schools in Guizhou Province to conduct a self administered questionnaire survey. Unconditional binary Logisitic regression was used to analyze the relationship between dieting, binge eating behaviours, and the co-occurrence of the two and NSSI among secondary vocational school students, and were stratified by sex.
Results:
The detection rate of NSSI among secondary vocational school students in Guizhou Province was 27.5%, 6.8% of secondary vocational school students reported dieting, 10.5% reported binge eating behaviour, and 2.9% showed both dieting and binge eating behaviours. The adjusted model of unconditional binary Logistic regression showed that the presence of dieting ( OR =2.37), binge eating behaviour ( OR =2.31), and the co-occurrence of both ( OR =2.60) were associated with higher risk of NSSI among secondary vocational school students; stratified analysis by gender showed that both males and females with dieting, binge eating and coexistence of dieting and binge eating behaviours were at increased risk of NSSI (females: OR =2.27, 2.26, 2.78 ; males: OR =2.15, 2.08, 2.02) ( P <0.01).
Conclusions
Dieting and binge eating behavior of secondary vocational school students will increase the risk of NSSI. When preventing and intervening NSSI problems of secondary vocational school students, it should pay more attention to eating habits.
9.CHINET 2012 surveillance of antibiotic resistance in Klebsiella spp .in China
Jing GUAN ; Chao ZHUO ; Danhong SU ; Yuxing NI ; Jingyong SUN ; Fu WANG ; Demei ZHU ; Fupin HU ; Yingchun XU ; Xiaojiang ZHANG ; Yunsong YU ; Qing YANG ; Zhongju CHEN ; Ziyong SUN ; Zhaoxia ZHANG ; Ping JI ; Bin SHAN ; Yan DU ; Hong ZHANG ; Jing KONG ; Yuanhong XU ; Jilu SHEN ; Chuanqing WANG ; Aimin WANG ; Zhidong HU ; Quan LI ; Lianhua WEI ; Ling WU ; Yunjian HU ; Xiaoman AI
Chinese Journal of Infection and Chemotherapy 2014;(5):398-404
Objective To investigate the antimicrobial resistance of clinical strains of K lebsiella spp .isolated from 15 hospitals in China CHINET during 2012 .Methods Kirby-Bauer method and automatic microbiology analysis system were employed to study the antimicrobial resistance . WHONET 5 .6 software was applied for data analysis according to Clinical and Laboratory Standards Institute (CLSI) 2012 breakpoints .Results A total of 9 621 clinical K lebsiella isolates were analyzed ,including 8 772 strains of K . pneumoniae and 804 strains of K . oxytoca . About 54 .9% (5 285/9 621) of the K lebsiella strains were isolated from sputum ,and 16 .3% (1 564/9 621) were isolated from pediatric patients .Antimicrobial susceptibility testing showed that about 8 .9% ,10 .8% and 12 .9% of the strains were resistant to imipenem ,meropenem and ertapenem ,respectively .About 14 .1% and 17 .0% of the strains were resistant to piperacillin-tazobactam and cefoperazone-sulbactam , respectively . Carbapenem-resistant K lebsiella strains were identified from all the 15 hospitals ,including 945 strains of K .pneumoniae and 45 strains of K .oxytoca ,which were resistant to either imipenem ,meropenem or ertapenem .Conclusions The Klebsiella isolates collected from 15 hospitals in China during 2012 are relatively sensitive to carbapenems ,cefoperazone-sulbactam and piperacillin-tazobactam .The prevalence of carbapenem-resistant strains is still increasing in China ,about 10 .3% in 2012 ,and relatively higher in Eastern China .More efforts should be made to control the superbug .
10.CHINET 2012 surveillance of antibiotic resistance in Enterobacter spp .in China
Zhongju CHEN ; Ziyong SUN ; Zhidong HU ; Jin LI ; Lianhua WEI ; Ling WU ; Yingchun XU ; Xiaojiang ZHANG ; Yuanhong XU ; Jilu SHEN ; Chuanqing WANG ; Aimin WANG ; Yuxing NI ; Jingyong SUN ; Zhaoxia ZHANG ; Ping JI ; Fu WANG ; Demei ZHU ; Fupin HU ; Yunzhuo CHU ; Chao ZHUO ; Danhong SU ; Yunsong YU ; Jie LIN ; Hong ZHANG ; Jing KONG ; Yunjian HU ; Xiaoman AI ; Bin SHAN ; Yan DU
Chinese Journal of Infection and Chemotherapy 2014;(5):387-391
Objective To investigate the distribution and antibiotic resistance of clinical Enterobacter isolates .Methods A total of 3 031 clinical strains of Enterobacter were collected from 15 hospitals from January 1 through December 31 , 2012 . Antimicrobial susceptibility testing was performed with Kirby-Bauer or minimum inhibitory concentration method .The results were analyzed according to CLSI 2012 breakpoints .Results Enterobacter cloacae and Enterobacter aerogenes accounted for 73 .0% (2 212/3 031) and 23 .9% (725/3 031) of all the Enterobacter strains .The isolates of other Enterobacter species accounted for 3 .1% (94/3 031 ) . The main source of the isolates was respiratory tract specimen , accounting for 53 .2% (1 612/3 031) .Most (> 89% ) of the Enterobacter strains were resistant to cefazolin and cefoxitin . Generally ,54 .4% ,47 .5% and 34 .3% of the strains were resistant to cefuroxime ,cefotaxime and cefazidime ,respectively . About 6 .6% to 26 .3% of the strains were resistant to amikacin ,gentamicin ,piperacillin-tazobactam ,cefepime ,cefoperazone-sulbactam ,ciprofloxacin and trimethoprim-sulfamethoxazole .Imipenem ,meropenem and ertapenem showed the highest activity , to which only 3 .5% ,3 .7% and 10 .3% of the strains were resistant ,respectively .About 8 .9% (269/3 031) of the strains were resistant to at least imipenem ,meropenem or ertapenem .Four Enterobacter strains were extensive-drug resistant (XDR) .Conclusions The prevalence of antibiotic resistance in Enterobacter isolates decreased slightly in 2012 compared to the data in 2011 ,but the situation is still very serious .We should continue to take effective measures to control the resistant strains .