This study was to examine the effects of blood sample preserved by different methods and times on blood lactate. The subjects were 25 male middle school students. Earlobe blood was collected and the blood lactate was determined respectively at once, after preserved by freezing 1 week, 2 weeks, 3 weeks, 4weeks or cold storage 1 day and 5 days. The results showed that all lactate values measured of 6 preserved samples were not significantly different but relevant with that of fresh sample.