Objective To investigate the therapeutic effect of combining hysterosalpingography and B ultra-phonic monitor for the follicle on the infertilitas feminis.Methods 146 infertility patients were randomly divided into observation group and control group according to digital table method,73 cases in each group.Patients in control group were treated with comprehensive optimization of treatment,while the observation group received fallopian canal side of progesterone based on the integrated optimization of treatment.All patients underwent uterine oviduct radiography,and the observation group used the fallopian tube with contrast -enhanced ultrasound in monitoring follicle to identify patients with dominant follicle.The pregnancy rate was compared between two groups.Results 146 cases of infertility patients with the uterus and fallopian tube radiography inspection found hydrosalpinx in 39 cases,accounted for 26.71%,29 cases of tubal blockage,accounted for 19.86%,tubal and the poor in 51 cases (34.93%),fallopian tube was through 27 cases (18.49%).There was no significant difference in the contrast between the two sides of the fallopian tube in the observation group and the control group (P >0.05).The intrauterine pregnancy rate of the obser-vation group (41.10%)was significantly higher than the control group (20.55%),while the ectopic pregnancy rate (28.77%)was significantly lower than the control group (50.68%)(χ2 =7.23,7.32,all P <0.05).The ectopic pregnancy in patients with tubal obstruction was significantly higher than that of tubal obstruction,tubal obstruction and tubal tubal type (χ2 =10.57,5.92,13.85,all P <0.05).Conclusion Combining hysterosalpingography and B ultraphonic monitor can accurately predict the date of ovulation,help guide the pregnancy intervention measures, improve the infertility of intrauterine pregnancy rate and prevent the occurrence of ectopic pregnancy.

OBJECTIVE To explore the clinical distribution and drug resistance of extended-spectrum ?-lactamases(ESBLs) producing Enterobacter cloacae.METHODS The clinical distribution and drug resistance of 73 strains of E.cloacae and ESBLs producing E.cloacae identified in our hospital from Jan 2002 to Jan 2004 were analyzed.RESULTS Among them,the highest detectable rate was in respiratory department and from phlegm specimen.No ESBLs producing E.cloacae and non-(ESBLs) were resistant to imipenem;no non-ESBLs were resistant to cefepime,but some ESBLs producing E.cloacae was resistant to it;all ESBLs producing E.cloacae and non-(ESBLs) were resistant to ceftriaxone and amoxicillin/clavulanic acid.CONCLUSIONS E.cloacae is one of the(commonest) pathogens in nosocomial infection,and ESBLs producing E.cloacae has a high detectable rate,its drug resistance has increased;the clinic should choose antimicrobial agents rationally according to drug sensitive tests in vitro.

Objective To investigate Helicobacter pylori combined with N-methyl-N-nitrosourea (MNU) gavage for preparing balb/c mouse gastric cancer model.Methods Eighty balb/c mice were randomly divided into four groups after 1-week adaptive feed,normal group,model group Ⅰ,Ⅱ and Ⅲ,20 cases in each group.The model group Ⅰ,Ⅱ and Ⅲ were given Helicobacter pylori bacteria liquid (CFU=109/mL) gavage,once every other day for 5 times;then,the freshly configured MNU solution 0.15,0.3,0.6 mL gavages were in turn given,MNU and pure water allocation ratio was 5mg:3mL.Once gavage per week for continuous 10 weeks.Results The model group II had 66.67% adenocarcinoma,the model group I were gastritis with mild atypical hyperplasia,and all mice in the model group III died.Conclusion This method can prepare the gastric cancer model.

Objective To evaluate the significance of AFP-IgM, this is one of new tumor markers, in the diagnosis of primary hepatocellular carcinoma (PHC). Methods The contents of AFP-IgM and AFP in serum of 103 healthy subjects, 74 patients suffered primary hepatic carcinoma, 27 patients affected by liver cirrhosis and 63 patients affected by chronic hepatitis were detected by means of enzyme linked immunosorbent assay and electrochemiluminescence. No-PHC is comprised of liver cirrhosis,chronic hepatitis and health subjects as control group. Results The area under ROC curve of AFP was larger than that of AFP-IgM (0.85 vs 0.72, Z=3.21) and the best cut-off value of AFP-IgM and AFP was 3×105-AU/L and 10 ug/L respectively, which was determined by ROC curve. Under the cut-off value, the sensitivity of AFP- lgM and AFP for PHC were 64.9% and 79.7%, and the specificity were 75.6% and 80.3%, yet their efficacies were similar. However, for early diagnosis of liver cancer (stage Ⅰ and Ⅱ), the area under ROC curve of AFP-IgM was larger than that of AFP (0.91 vs 0.82,Z=1.73). The sensitivity of AFP-IgM andAFP were 94.4% and 72. 2%, and the specificity were 81.9% and 79.9%. The differences of AFP-IgMand AFP for early diagnosis of liver cancer were statistically significant. When both of the test results combined AFP-IgM with AFP are positive, it can be diagnosed as liver cancer. The specificity of combineddetermination of the two forms was 89.1%, and the efficacy was 79. 0%. Conclusions Both of thesensitivity and specificity of the AFP-IgM test were higher than that of the AFP for early diagnosis of livercancer. We also found that combined determination of the two forms significantly increased the specificityand the positive predictive value for the diagnosis of PHC, thus AFP-IgM was of especially significance forearly diagnosis of liver cancer.