Objective To investigate the negative effect of cigaratte smoking on periodontal theraphy. Mothods 84 patients with periodontopathy were selected and divided into two groups. The smoking group (group A) included 33 patients and the non-smoking group (group B) included 51 patients. All the patients received a systematic treatment. The effects of the treatment between different groups were compared through the debris index(DI),calculus index(CI),periodotal disease index(PDI) which were respectively recorded before and after treatment.Results After the treatment the DI,CI,PDI of two groups were all decreased; wheras compared with group A, every index of group B was significantly lower. Conclusions Somking seriously influences the effect of periodontal therapy. Therefore, to give up smoking is very important for preventing and treating periodontopathy.

Background and purpose:Acinar cell carcinoma of the pancreas (ACCP) is a rare malignant tumor and a few radiologic reports have been published. This study aimed to evaluate the CT characteristics of ACCP. Methods:CT signs of 9 cases of pathologically conifrmed ACCP were analyzed retrospectively.Results:The mean value of longest diameter of the 9 cases of ACCP was 52 mm. Among the 9 cases, 6 cases (66.7%) had ill-deifned bor-der, 6 cases (66.7%) showed exophytic type, 8 cases (88.9%) showed enhancement degree less than normal pancreatic tissue, 6 cases (66.7%) represented heterogeneous enhancement, 7 cases (77.8%) showed invaded vessel, 5 cases (55.6%) had lymph node metastasis, and none had hepatic metastasis. Dilated pancreatic duct was observed in only 1 case. Conclusion:When pancreatic mass is large, heterogeneous, exophytic and without dilated pancreatic duct, ACCP is suggested.

Objective To investigate the stress distributions on implant-bone interface and fatigue behaviors of biomimetic titanium implant under static and dynamic loading conditions to provide theoretical basis for a new implant which may effectively transfer the stress to surrounding bones. Methods A 3-D finite element model of a posterior mandible segment with an implant bone was constructed by a CAD (Pro/E Widefire 2.0) software. Two different implant models (a dense implant No.1 and a biomimetic implant No.2) were designed. The stress distributions on bone-implant interface under dynamic and static loading conditions were analyzed by Ansys Workbench 10.0 software, as well as the fatigue beha-vior of the biomimetic implant. Results The cervical cortical bones in the 2 implants were all high stress region under the same loading condition. The maximum von Mises stress on the interface and high-stress region in the cancellous bone region, and the maximum stress in the root region of the biomimetic implant were lower than those of the dense implant. The stress on the implant-bone interface decreased from the top to the bottom. The stress in the cervical cortical bone under the dynamic loading was 17.15% higher than that of the static loading. There was no significant difference in maximum stress at the cortical bone region between the dynamic and static loading conditions. The maximum stress of the dense implant in the cancellous bone region was 75.97% higher and that in the root region was 22.46% higher than that of the biomimetic implant. The maximum stress on the implant-bone interface was far less than the yield strength of pure titanium. The stress distribution in the cortical region of the biomimetic implant was 7.85% higher than that of the dense implant, and the maximum stress in the cortical bone was smaller than the yield stress of cortical bone. Within the dynamic loading of 50-300 N, the safety coefficient was all higher than 10, and with the increase of loading pressure, interface stress in the cancellous region increased linearly. Under the loading of 300 N in the axial and 25 N in the lingual 45°, the maximum stress was 11.38 MPa. Conclusion Biomimetic style implant can effectively transfer the implant-bone interface stress to surrounding bones in the cancellous bone and root region, and the structure with the improved design is safe under normal loading pressure.

Objective To establish the culture technology system for the in-vitro induction of adventitious buds of Sarcandra glabra(Thumb.) Nakai by investigating the induction factors of in-vitro adventitious buds.Methods Stem segments of Sarcandra glabra were cultured as explants.The effects of culture medium with different concentrations of 6-benzylaminopurine(6-BA) and indole butyric acid(IBA) added,lighting time,light intensity,and temperature on the induction of adventitious buds from the explants were observed.Results The medium containing both 6-BA and IBA was more effective to induce shoot formation of Sarcandra glabra than the medium only containing 6-BA or IBA;high concentration of plant hormones would block the growth of the plantlets.The scattering light or faint direct light was suitable for the shoot formation,while dark or strong light would induce the plantlets withered or even dead.Temperature in the range of 25 ℃～30 ℃ was beneficial for the growth of adventitious buds,and the bud induction rate arrived 100% at the temperature of 25 ℃.Conclusion At the temperature of 25 ℃,the faint sunlight or direct light less than 2000 lux,and the medium with 2.5 mg?L-1 6-BA and 0.1 mg?L-1 IBA added can stimulate significantly the adventitious bud formation of Sarcandra glabra,the induction rate up to 82.98%.

OBJECTIVETo investigate the effects of Ginsenoside Rb(1) on the proliferation of Schwann cells in culture.

METHODSApplying MTT assay and Thymidine incorporation assay, the effects of Ginsenoside Rb(1) on the proliferation of Schwann cells isolated from the sciatic nerve of adult rat were studied.

RESULTSGinsenoside Rb(1) (10 microg/ml) significantly induced Schwann cell proliferation, the effect was similar to NGF (50 microg/ml). At high concentrations of Ginsenoside Rb(1) (1 mg/ml), the proliferation of Schwann cells was significantly inhibited.

CONCLUSIONSGinsenoside Rb(1) at the optimal concentrations is found to be effective in inducing the proliferation of Schwann cells, but at higher concentrations the drug is cytotoxic for Schwann cells.

Animals ; Cell Division ; drug effects ; physiology ; Cells, Cultured ; Dose-Response Relationship, Drug ; Ginsenosides ; adverse effects ; pharmacology ; Immunohistochemistry ; Nerve Regeneration ; drug effects ; Probability ; Rats ; Rats, Inbred Strains ; Schwann Cells ; drug effects ; physiology ; Sciatic Nerve ; cytology ; physiology ; Sensitivity and Specificity

Recent studies have identified that metabolic reprogramming in lung adenocarcinoma cells, particularly lactate metabolism disorders, plays a crucial role in tumor development and immune therapy response. The accumulation of lactic acid not only provides energy support for the proliferation of tumor cells but also affects the function of immune cells by changing the tumor microenvironment, thereby promoting immune escape. Immunotherapy, especially the application of immune checkpoint inhibitors, has become an important strategy for treating lung adenocarcinoma. However, lactate metabolism disorders might affect the efficacy of immunotherapy, leading to resistance in some patients. Therefore, a thorough understanding of the mechanisms of lactic acid metabolism in lung adenocarcinoma and its impact on the response to immunotherapy is essential for developing new therapeutic strategies and improving the efficacy of immunotherapy. This review summarizes the role of lactate metabolism disorders in the development and immunotherapy of lung adenocarcinoma, discusses the potential role of lactic acid metabolism-related genes and pathways in lung adenocarcinoma, and explores the progress in therapeutic strategies targeting lactic acid metabolism regulation. This work aims to provide new insights for the treatment of lung adenocarcinoma.