Objective To investigate the requirement status and effect of relevant knowledge education in patients undergoing painless colonoscopy examination. Methods 230 cases of patients undergoing painless colonoscopy were chosen.The requirement status of relevant knowledge of painless colonoscopy examination was taken for investigation.The patients were randomly divided into the control group (90 cases) and the observation group( 140 cases).The control group was given basic nursing of painless bowel endoscopy and passively answering the questions raised by patients.The observation group was given active,systematic and designed knowledge education and psychological support for patients and their families on the basis of the control group.The knowledge level,coordination degree with examination and satisfaction degree of patients with painless colonoscopy were taken for statistics. Results The patients and their families were most concerned about security of painless colonoscopy,followed by instruction and compliance points before and after the examination,and the knowledge of anesthesia was rarely concerned about.The related knowledge of painless colonoscopy for the two groups of patients before the examination improved significantly than that after newly diagnosed,but the observation group was better than the control group.The compliance and inspection time in the observation group were significantly better than the control group.The satisfaction degree of patients and their families in the observation group were significantly higher than the control group. Conclusions The security for painless colonoscopy and instruction and compliance points before and after the examination were urgently needed by patients.Systematic knowledge education has pivotal significance in ensuring examination quality,improving satisfaction degree of patients,which is worthy of clinical application.

Objective To discuss effect and nursing process of endoscopic biliary stent implantation for treatment of patients with malignant biliary obstruction.Methods To evaluate efficiency of the operation and to observe nursing situation of 47 cases treated by the way of endoscopic biliary stent implantation in our hospital.Results In 47 patients,31 patients used EMBE,16 patients were given ERBD,the total rate of treatment efficiency reached 100％.Positive and effective nursing measures were given to two groups during operation period,which ensured the smooth process and increase the success rate of operation.Conclusions Endoscopic biliary stent placement for malignant biliary obstruction has gradually become an effective substitution of normal operations.And active and reasonable nursing can increase the success rate of operation and decrease postoperative complications.

Objective To investigate the effects of health education on enhancing the compliance behavior of patients with diabetes mellitus (DM). Methods 63 DM patients were provided with systemic health education during their staying in the hospital. A follow-up interview was performed at the 6th month of discharge to gather the information of how importance of compliance behavior on the prognosis of DM. Result After the systemic health education, the patients could take medicine on time and had a good behavior in daily life, which will effectively improved their quality in the future life.Conclusion Systemic the health education can improve the compliance behavior of patients, and thus directly affects the prognosis of DM.

Objective To explore the therapeutic effect on the prevention of eccyesis by optimizing fallopian tube to be pregnant.Methods Three hundred and seventy-nine patients with infertility were divided into two groups:control group with 187 patients received the combined optimized treatment,and study group with 192 patients received the combined optimized treatment and optimizing fallopian tube to be pregnant.All patients received the hysterosalpingography uterosalpingography(HSU)whik the patients of the study group received the ultraphonic monitor for the.dominant follicle besides for 6 ovulatory cycle.Results There were intrauterine gestation 59 patients(31.55%,59/187),eccyesis 20 patients(10.70%,20/187)and infertility 108 patients(57.75%,108/187)in control group while there were intrauterine gestation 83 patients(43.23%,83/192),eccyesis 10 patients(5.21%,10/192)and infertility 99 patients (51.56%,99/192)in study group.The intrauterine gestation rate in study group was higher than that in control group(P<0.05),and the eccyesis rate was lower than that in control group(P<0.05).But there was no significant difference in the infertility rate between two groups(P＞0.05).The eccyesis rate in fallopian tube unsmooth was higher than that in salpingemphraxis and fallopian tube canalization respectively[12.59%(18/143),2.63%(2/76),2.70%(2/74),P<0.05].Conclusion Selecting the optimizing fallopian tube by HSU and monitoring the dominant follicle by ultraphonic to guide pregnancy can let the dominant follicle grow and conceive in the optimizing fallopian tIlbe,and this treatment can raise the intrauterine gestation rate and decrease the cause of eccyesis.

Objective To analyze the difference of salivary microbiome between the patients with functional dyspepsia and healthy controls.Methods Saliva samples were collected from the patients with functional dyspepsia and healthy control.Genomic DNA of the samples was extracted,and the high-throughput sequencing technology was used to conduct DNA sequence of 16S rRNA-V4 region.Subsequently,all the data were performed by the bioinformatic analysis.Results The salivary microbiome in the functional dyspepsia group was dominated by Proteobacteria,while Bacteroidetes was the top microbiota in the heathy control group.In the functional dyspepsia group,the Chao1 index,ACE index,Shannon index and Simpson index were 1 295,1 351,4.93 and 0.90 respectively.In the healthy control group,the above indexes were 1 001,1 351,5.28 and 0.92 respectively.The PCoA ba sically separated the microbiome composition of the two groups.Sixteen kinds of microbiota were significantly different between two groups using linear discriminant analysis effect size tool,including Bacteroidetes,Prevotella,Prevotellaceae,Neisseria,Betaproteobacteria and Proteobacteria,etc.Conclusion Saliva in the patients with functional dyspesia has characteristic microbiome composition,which is dominated by Proteobacteria,but the richness and diversity between the two groups have no difference.Neisseria is one of the significantly different bacteria between the two groups.

Objective To evaluate the association between the single-nucleotide polymorphism (SNP) of the 5-59A/G (rs2241027) of Htra2 gene and Parkinson's disease in Han population of the western GuangDong province. MethodsThe restriction fragment length polymorphism (PCR-RFLP) was used to determine the 5-59A/G polymorphism in the intron region of Htra2 gene in the case group (n=56) and healthy control group (n=109). ResultsA allele frequency of 5-59 A/G in cases (46.4%) was trended to more than that in controls (36.7%) (P=0.073), as well as the AA genotyping frequency (21.4% vs 11.0%, P=0.072). For the male, the frequency of AA genotype was significantly more in cases (25.7%) than that in controls (10.3%） (P=0.041), and the frequency of A allele was trended to more in cases (48.6%) than in controls (34.6%) (P=0.051). ConclusionA allele and AA genotype of the 5-59A/G (rs2241027) of Htra2 gene may increase the risk of suffering from Parkinson's disease, especially for males.

Objective The objective of this study was to investigate the relationship between serum long-chain non-cod-ing RNA MALAT1 and AFAP1-AS1 and clinicopathological features,and prognosis of nasopharyngeal carcinoma(NPC). Methods A total of 136 patients with nasopharyngeal carcinoma confirmed by pathology in our hospital were selected from April 2013 to June 2015. During the same time,54 outpatients for health examination in our hospital were selected as the control group and nasopharynge-al carcinoma group. Real-time fluorescence reverse transcription analysis was used to analyze the expression of lncRNA MALAT1 and AFP1-AS1. The relationship between the expression of lncRNA MALAT1 and AFP1-AS1,and clinicopathological characteris-tics was analyzed by χ2 test. Log-rank assay was used to analyze serum long-chain non-coding RNA MALAT1 and prognostic differences in patients with different expression levels of AFAP1-AS1. Results Compared with the control group,the serum levels of RNA MALAT1 and RNA AFAP1-AS1 in the nasopharyngeal carcinoma group were significantly increased(P<0. 001);The expres-sions of lncRNA MALAT1 and AFAP1-AS1 was not related to age(P>0. 05);The maximum diameter of the tumor was≥5 cm,the pathological stage was higher,the TNM stage was higher,the deeper in the infiltration depth,the infiltration of lymphatic vessels,the lymph node metastasis,and the recurrence and the higher in high expressive rates of lncRNA MALAT1 and AFAP1 -AS1 ( P <0. 05). The 2-year survival rate and survival time of lncRNA MALAT1 and AFAP1-AS1 in the low expression group were signifi-cantly higher than those of the high expression group(P<0. 001);Multivariate Cox stepwise regression analysis showed that low ex-pression of lncRNA MALAT1(HR=0. 52,95% CI:0. 37~0. 81)and low expression of lncRNA AFAP1-AS1(HR=0. 56,95% CI:0. 51~0. 83)were independent prognostic protective factors for NPC patients(P<0. 001). Conclusion The serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 are elevated in patients with NPC,and are positively correlated with malignant progression of NPC. NPC patients with low expression of lncRNA MALAT1 and AFAP1-AS1 serum have a good prognosis;MALAT1 and AFAP1 -AS1 can be used as new markers for the diagnosis of nasopharyngeal carcinoma.

Protein tyrosine phosphatase H-type receptor (PTPRH) gene encodes a gastric cancer associated protein, which exerts its biological function through tyrosine phosphorylation in the post-translational COOH- terminal region. PTPRH is abnormally expressed in a variety of tumors, and its biological function is closely related to the occurrence, development and prognosis of tumors.
Humans ; Phosphorylation ; Protein Tyrosine Phosphatases ; Proteins ; Stomach Neoplasms ; Tyrosine

Objective To evaluate the effect of surgery-radiotherapy interval (SRI) on clinical prognosis of locally advanced stage c Ⅱ-Ⅲ breast cancer patients treated with neoadjuvant chemtherapy and modified radical mastectomy.Methods Clinical data of 1 087 breast cancer patients treated with neoadjuvant chemotherapy and modified radical mastectomy from 11 hospitals in China were retrospectively analyzed.The optimal threshold value of SRI upon clinical prognosis was determined by maxstat method.The effect of SRI on clinical prognosis was evaluated by using multivariate Cox regression analysis and propensity score matching (PSM).Results The median follow-up time was 72.9 months.The 5-year disease-free survival (DFS) and overall survival (OS) rates were 68.1％ and 81.8％.All patients were divided into SRI ≤18 weeks (n=917) and SRI＞ 18 weeks groups (n=170).Multivariate Cox regression analysis demonstrated that hormone receptor status (P＜0.001),pathological T stage (P＜0.001),pathological N stage (P＜0.001) and SRI (P=0.023) were independent influencing factors of DFS.Hormone receptor status (P=0.013),pathological T stage (P=0.006),pathological N stage (P＜0.001),endocrine therapy (P=0.013) and SRI (P=0.001) were significantly associated with OS.After balancing the clinical and pathological factors with PSM,patients with SRI＜ 18 weeks had superior DFS and OS to those with SRI＞ 18 weeks.Conclusions SRI affects the clinical prognosis of locally advanced breast cancer patients treated with neoadjuvant chemotherapy and modified radical mastectomy.Radiotherapy should be performed within 18 weeks after mastectomy.