Objective To study the effect of rosiglitazone, a ligand of peroxisome proliferator-activated receptor gamma (PPAR?), on the expression of PPAR? in hepatic stellate cells (HSCs) and its effect on the biological characters of HSCs. Methods The activated HSCs were devided into three groups:control, 3??mol/L rosiglitazone group, and 10??mol/L rosiglitazone group. The expression of PPAR?, ?-smooth muscle actin(?-SMA), and type Ⅰ and Ⅲ collagen was detected by means of RT-PCR, Western blot and immunocytochemistry respectively. The cell proliferation was determined with MTT colorimetric assay. The cell apoptosis was demonstrated with flow cytometry. Results The expression of PPAR? at mRNA and protein level markedly increased in HSCs of 10??mol/L rosiglitazone group(t≥4.627, P

OBJECTIVE To establish a method for the simultaneous determination of 2 specific phenolic acids glycosides 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose in Isatidis Folium. METHODS The HPLC analysis was carried on Elite C18 column(4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution(gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was 328 nm, and the column temperature was set at 30 ℃. RESULTS 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose were showed good linear relationships within the ranges of 2.092-104.6 μg·mL-1(R2=0.999 6) and 2.996-149.8 μg·mL-1(R2=0.999 5). The average recovery (n=6) were 99.05% and 99.60%, RSDs were 0.34% and 0.65%, respectively. CONCLUSION The established method is simple, reliable and specific, and suitable for simultaneous determination of two components of phenolic acids glycosides in Isatidis Folium.