1.Distribution and change in antimicrobial resistance of pathogens from sputum of hospitalized children in a pediatric department in 3 consecutive years
Rong HUANG ; Liangbin HUANG ; Yingmin TIAN ; Jinjin WANG
Chinese Journal of Infection Control 2015;(4):236-239
Objective To realize the distribution and change in antimicrobial resistance of common pathogens causing acute lower respiratory tract infection (LRTI)in children,and provide evidence for rational use of antimicrobial agents. Methods Data about pathogens from children with LRTI in a hospital between January 2011 and December 2013 were ana-lyzed statistically.Results Of 934 isoalted pathogenic strains,728 (77.94%)were gram-negative bacteria,the major were Klebsiella pneumoniae (n =278),Escherichia coli (n =216),Enterobacter cloacae (n =85 ),and Pseudomonas aeruginosa (n=63).The isolation rate of gram-positive bacteria was 20.87%(n=195),the major were Staphylococcus aureus (n=132)and Streptococcus pneumoniae (n=49).Antimicrobial susceptibility testing results revealed that sensitive rate of gram-negative bacteria to imipenem,meropenem,and amikacin were all 100.00%,to ceftazidime/clavulanic acid and piperacillin/tazobactam were relatively low,to cephalosporins increased year by year.Sensitive rates of the main gram-positive bacteria to vancomycin were both 100.00%, to erythromycin and clindamycin were relatively low. Conclusion The major pathogenic bacteria causing LRTI in pediatric department are gram-negative bacteria,antimicrobial resistance rates increased year by year,management of antimicrobial use in children should be strengthened to prevent the occurrence of multidrug-resistant organism infection.
2.The application of lentil lectin-reactive alpha-fetoprotein ratio in the differential diagnosis of primary liver cancer
Xiaoling ZHAO ; Jingjing WANG ; Qiaoyu ZHAO ; Liangbin HUANG
International Journal of Laboratory Medicine 2016;37(9):1228-1229,1231
Objective To explore the application of lentil lectin‐reactive alpha‐fetoprotein ratio (AFP‐L3% ) applied in the dif‐ferential diagnosis between hepatitis B infection of primary liver cancer and benign liver disease .Methods We included 108 cases of chronic HBV infection ,including 50 cases of primary liver cancer ,42 cases of cirrhosis ,16 cases of chronic hepatitis .Chemilumines‐cence detection was used to detect alpha‐fetoprotein (AFP) and AFP‐L3 content ,AFP‐L3 and the ratio of AFP (AFP‐L3% ) was calculated .Results AFP≥400 ng/mL as primary liver cancer diagnostic threshold ,the sensitivity and specificity were 36% ,84% , when used AFP‐L3% ≥ 10% as primary liver cancer diagnostic threshold ,the sensitivity and specificity were 62% ,83% . Conclusion AFP‐L3% is a better clinical indicator to distinguish between primary liver cancer and benign liver disease .AFP‐L3%can be used as a clinical indicator to differential diagnosis between HBV infection of primary liver cancer and benign liver disease .
3.Allogeneic blood transfusion alleviates hip joint pain induced by ankylosing spondylitis
Xin ZHANG ; Peng WANG ; Yanfeng WU ; Rui YANG ; Lin HUANG ; Yong TANG ; Yuxi LI ; Liangbin GAO ; Huiyong SHEN
Chinese Journal of Tissue Engineering Research 2014;(9):1465-1470
BACKGROUND:Pain is the main clinical manifestation for ankylosing spondylitis. At present, nonsteroid anti-inflammatory drugs are oral y taken, but the effects are limited and toxic and side effects are more. Thus, there is no effective scheme for treatment of pain induced by ankylosing spondylitis.
OBJECTIVE:To investigate the correlation between postoperative joint pain al eviation and al ogeneic blood transfusion, and the mechanisms.
METHODS:We retrospectively analyzed clinical data of 88 ankylosing spondylitis patients combined with kyphosis who received only one section of osteotomy surgery merging hip joint pain. We compared the visual analog scale score of hip joint and detected the variation of leucocytes, lymphocytes and immunoglobulin concentrations before and after the operation in the groups of fresh al ogeneic whole blood transfusion, autologous whole blood transfusion, and mixed transfusion of al ogeneic and autologous whole blood. Flow cytometry was used to analyze the number and ratio of peripheral blood Th17 cells and Treg cells which were both highly associated with autoimmune diseases.
RESULTS AND CONCLUSION:The symptom of hip arthralgia obviously improved in both groups transfused by fresh al ogeneic whole blood or al ogeneic-autologous mixed whole blood. However, no obvious variation was detected in leucocytes, lymphocytes and immunoglobin concentration. However, flow cytometry results indicated that Th17/Treg proportion associated with autoimmune diseases was increased remarkably in peripheral blood of ankylosing spondylitis patients. Results suggested that al ogeneic whole blood transfusion can al eviate patients’ joint pain by correcting the imbalance of Th17/Treg which may improve their immune state.
4.KINETIC CHANGES OF ANTIBODY TO M.LEPRAE AND ITS RELATION WITH CLINICAL DISEASE
Jianping SHEN ; Wenzhong LI ; Ganyun YE ; Wanhui WEI ; Liangbin YAN ; Xiangsheng CHEN ; Wenbiao HUANG ; Fenwu LIU ; Shunpeng RAN
Chinese Medical Sciences Journal 1999;(1):52-56
A sero-epidemiological survey on 1 833 healthy residents was carried out in 6 villages of a leprosy high-endemic area in Wenshan and Guangnan counties, Yunnan Province. The part of the residents with initially antibody-positive as well as the part of residents with initially antibody-negative have been followed up for 3 consecutive years by serology and clinical examination for studying kinetic changes of antibody to M.leprae and its relation with clinical disease. The results showed that the rates of subclinical infection of leprosy in a high-endemic area are different from village to village, and the risk of developing clinical disease does not associate with subclinical infection rate. It correlates with the number of cured accumulative leprosy cases and active cases within the village. The authors consider that in leprosy high-endemic villages, especially those cropped up new multi-bacillary leprosy cases frequently in recent years, it may be helpful to use serology to detect early leprosy cases.
5.Effect of pterostilbene on apoptosis and glycolysis of ovarian cancer SKOV3 cells and its mechanism
Li HUANG ; Youmei LONG ; Yixia FU ; Liangbin XIA
Practical Oncology Journal 2019;33(6):502-507
Objective The aim of this study was to investigate the effect and mechanism of pterostilbene on apoptosis and glycolysis of ovarian cancer SKOV3 cells. Methods SKOV3 cells were treated with 0,25,50,100 and 150 μmol/L of pterostilbene for 24,48 and 72 hours. The proliferation of SKOV3 cells was measured by CCK. The effect of pterostilbene on apoptosis of SKOV3 cells was determined by flow cytometry. The glucose consumption and lactate production were detected by glucose oxidase assay and chemical colorimetry. The expression of signal transducer and activator of transcription 3 ( STAT3 ), phosphorylated STAT3 ( p -STAT3) and hexokinase 2 ( HK2) proteins was detected by Western blot. The expression of glucose transporter 1 ( GLUT1) and M2 pyruvate kinase(PKM2)mRNA was detected by qRT-PCR. Results Pterostilbene inhibited the proliferation of SKOV3 cells in a time- and dose-dependent manner. According to CCK-8 results,100 μmol/L of pterostilbene was selected as the follow-up ex-perimental group and 0 μmol/L as a control group. Pterostilbene could significantly promote the apoptosis of SKOV3 cells in a dose-dependent manner. The higher the concentration,the more obvious apoptosis effect,the difference was statistically significant ( P <0. 05). In addition,the levels of glucose consumption and lactate production in the 100 μmol/L group were significantly lower than those in the 0 μmol/L group(P<0. 01). The expression of p-STAT3 and HK2 protein in the 100 μmol/L group was also significant-ly lower than those in the 0 μmol/L group(P<0. 001). The expression of GLUT1and PKM2 mRNA in the 100 μmol/L group was also significantly decreased than those in the 0 μmol/L group(P<0. 01). Conclusion Pterostilbene can inhibit the proliferation of SK-OV3 cells and promote apoptosis,and may inhibit the glycolysis of ovarian cancer through a STAT3/HK2 pathway.
6.Diagnostic Utility of Electromagnetic Navigation Bronchoscopy Combined with Radial Probe Endobronchial Ultrasound in Peripheral Pulmonary Lesions
HUANG HAITAO ; CHEN SHAOMU ; PAN LIANGBIN ; CHEN KE ; YAO FEIRONG ; MA HAITAO
Chinese Journal of Lung Cancer 2017;20(12):837-840
Background and objective With the application of high resolution computed tomography (CT),a large number of peripheral lung lesions were found.It put forward new challenge on clinical diagnosis and treatment for these peripheral lung lesions.Electromagnetic navigation bronchoscopy (ENB) and radial endobronchial ultrasound probe (R-EBUS) are new technologies used for the diagnosis of peripheral lung lesions.The aim of this study is to explore the application value of ENB combined with R-EBUS in the diagnosis of peripheral pulmonary lesions.Methods From September 2016 to November 2017,eighteen patients with thirty peripheral pulmonary lesions in the First Affiliated Hospital of Soochow University were enrolled.The ENB was performed on these patients who were detected peripheral lung lesions by chest HR-CT.After successful navigation,the lesions location was confirmed by R-EBUS,and specimens were acquired by needle aspiration,endoscopic cell brush and biopsy forceps.Results A total of eighteen patients with thirty lesions were enrolled in this study,the navigation success rate was 100%,the positive rate was 90%.The mean operation time was (95.61±28.74) min,and navigation time for each lesion was (25.90± 11.29) min,and pneumothorax was observed in 1 case.Conclusion ENB combined with R-EBUS for the diagnosis of peripheral pulmonary lesions is safe and effective.This technique is worth promoting.
7.Multilevel Pedicle Subtraction Osteotomy for Correction of Thoracolumbar Kyphosis in Ankylosing Spondylitis: Clinical Effect and Biomechanical Evaluation
Xin LV ; Yelidana NUERTAI ; Qiwei WANG ; Di ZHANG ; Xumin HU ; Jiabao LIU ; Ziliang ZENG ; Renyuan HUANG ; Zhihao HUANG ; Qiancheng ZHAO ; Wenpeng LI ; Zhilei ZHANG ; Liangbin GAO
Neurospine 2024;21(1):231-243
Objective:
To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters.
Methods:
Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions.
Results:
Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO.
Conclusion
Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.
8.Multilevel Pedicle Subtraction Osteotomy for Correction of Thoracolumbar Kyphosis in Ankylosing Spondylitis: Clinical Effect and Biomechanical Evaluation
Xin LV ; Yelidana NUERTAI ; Qiwei WANG ; Di ZHANG ; Xumin HU ; Jiabao LIU ; Ziliang ZENG ; Renyuan HUANG ; Zhihao HUANG ; Qiancheng ZHAO ; Wenpeng LI ; Zhilei ZHANG ; Liangbin GAO
Neurospine 2024;21(1):231-243
Objective:
To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters.
Methods:
Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions.
Results:
Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO.
Conclusion
Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.
9.Multilevel Pedicle Subtraction Osteotomy for Correction of Thoracolumbar Kyphosis in Ankylosing Spondylitis: Clinical Effect and Biomechanical Evaluation
Xin LV ; Yelidana NUERTAI ; Qiwei WANG ; Di ZHANG ; Xumin HU ; Jiabao LIU ; Ziliang ZENG ; Renyuan HUANG ; Zhihao HUANG ; Qiancheng ZHAO ; Wenpeng LI ; Zhilei ZHANG ; Liangbin GAO
Neurospine 2024;21(1):231-243
Objective:
To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters.
Methods:
Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions.
Results:
Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO.
Conclusion
Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.
10.Multilevel Pedicle Subtraction Osteotomy for Correction of Thoracolumbar Kyphosis in Ankylosing Spondylitis: Clinical Effect and Biomechanical Evaluation
Xin LV ; Yelidana NUERTAI ; Qiwei WANG ; Di ZHANG ; Xumin HU ; Jiabao LIU ; Ziliang ZENG ; Renyuan HUANG ; Zhihao HUANG ; Qiancheng ZHAO ; Wenpeng LI ; Zhilei ZHANG ; Liangbin GAO
Neurospine 2024;21(1):231-243
Objective:
To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters.
Methods:
Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions.
Results:
Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO.
Conclusion
Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.