【Objective】To observe macular choroidal thickness and topographical variation in Chinese healthy and myopic children and to investigate the correlated factors. 【Methods】 A total of 196 myopic children treated at Hainan Province Eye Hospital were selected and divided into hyperopia ，emmetropia，low，moderate and severe myopia groups according to their spherical equivalents（SE）. Axial length（AXL），subfoveal choroidal thickness（SFCT）and 1 mm ，3 mm nasal，temporal，superior，and inferior to the foveal choroidal thickness were recorded. Forty- five students from Tung Wah Group of Hospital′s MA Kam Chan Memorial Primary School in Hong Kong were also recruited in this 1-year longitudinal study. Children were grouped to myopic group and emmetropic or hyperopic group according to SE ，thickness changes of choroid were compared among children with or without myopic shift. The correlation among choroidal SE，and AXL variations were also investigated.【Results】Mean SFCT was 250（204~314）μm. The choroid was thinner at the nasal and inferior sectors ，the thinnest being at 3mm nasal to the fovea ，and the thickest at 1mm temporal to the fovea. Choroidal thickness in all orientations became thinner with progressively descending refractive degree. SFCT and 1 mm around fovea decreased most compared with other surrounding directions in all groups（Kruskal- Wallis test，P < 0.05）. The choroidal thickness in subfovea and other sectors got thinned after 1 year follow-up time（Pair t test，P < 0.05）.The variation of the choroidal thickness in fovea and surrounding positions was positively correlated with the change of SE and negatively related to the change of the AXL. The myopic group had a faster descent of SE and a faster growth of AXL than the emmetropic or hyperopic group. SFCT and surrounding choroidal thickness showed a progressive descent in the myopic group，but a slight decrease in the emmetropic or hyperopic groups.【Conclusions】There is significant topographic variation of choroidal at different regions of the macular. The choroidal thickness decreases faster in myopes. SFCT is more sensitive to myopic progression compared with surrounding regions.

[摘 要] 目的：采用生物信息学方法探索与肾透明细胞癌(ccRCC)组织中铁死亡相关的lncRNA，并探讨其与免疫细胞浸润及患者预后的相关性，为ccRCC患者提供新的分子靶点。方法：从癌症基因组图谱(TCGA)数据库下载ccRCC的转录本数据和临床数据，利用单样本基因集富集分析(ssGSEA)及相关性分析获得与铁死亡相关的lncRNA；通过单因素和多因素回归分析构建与铁死亡相关的lncRNA特征图，分析其与预后的关系；利用R软件分析铁死亡相关lncRNA与肿瘤免疫细胞浸润和药物敏感性之间的关系。构建铁死亡相关RNA网络，并通过qPCR验证中国人ccRCC组织和癌旁组织（取自2019年12月至2021年03月间在西南医科大学附属医院手术切除8例标本）中关键lncRNA的表达。结果：Kaplan-Meier分析表明，铁死亡评分高的患者总OS率低于铁死亡评分低的患者。单因素和多因素回归分析确定11个ccRCC铁死亡相关lncRNA可评估患者预后，并构建ccRCC患者1、3、5年预后预测列线图。免疫细胞浸润分析表明，铁死亡相关lncRNA与ccRCC免疫细胞浸润密切相关，其中LINC01871、PRKAR1B-AS1和CYTOR是调节肿瘤免疫细胞浸润的关键lncRNA。化疗药物敏感性分析表明，高风险患者对甲氨蝶呤、紫杉醇、顺铂和多柔比星更为敏感。构建的包含3个lncRNA、15个miRNA和15个mRNA的RNA网络中，验证实验显示LINC01871、LINC00472和CYTOR在ccRCC组织中显著上调。结论：通过生物信息学方法获得11个与铁死亡相关的lncRNA，证明其与ccRCC组织免疫细胞浸润、化疗药物敏感性和患者预后相关，为探索ccRCC铁死亡相关lncRNA标志物提供重要参考。

[摘 要] 目的：阐明牙龈卟啉单胞菌（Pg）诱导食管鳞状细胞癌（ESCC）细胞发生上皮间质转化（EMT）的分子机制。方法：KEGG分析Pg诱导的ESCC差异表达基因富集的生物学通路，WB和/或免疫荧光法检测Pg诱导的ESCC细胞中糖蛋白A重复优势蛋白（GARP）、TGF-β、pSMAD/SMAD、Snail、Oct4和EMT相关分子表达的变化，ELISA检测TGF-β1水平的变化，免疫组织化学法检测ESCC组织中GARP和TGF-β1的表达规律，Transwell实验和动物实验验证Pg对ESCC的促进作用。结果： ESCC细胞感染Pg后，TGF-β、Hippo、PI3K/Akt等信号通路被激活；Pg感染刺激ESCC细胞分泌总TGF-β1和活性TFG-β1的水平升高（均P<0.01），使SMAD2/3磷酸化并发生核转位，诱导N-cadherin、Snail、Oct4等蛋白表达升高、E-cadherin蛋白表达降低，由此促进ESCC细胞的迁移、侵袭和裸鼠皮下移植瘤的生长（均P<0.01）。在ESCC细胞中沉默GARP表达后，逆转了Pg所诱导的上述ESCC细胞表型变化。Pg丰度高的ESCC组织中TGF-β1和GARP蛋白表达高于低丰度的ESCC组织，且Pg丰度与TGF-β1、GARP表达存在正向关联（P=0.001 5）。结论：Pg通过GARP激活TGF-β/SMAD轴促进ESCC细胞发生EMT，进而促进ESCC细胞的迁移、侵袭和生长，清除Pg或阻断TGF-β信号转导则可阻断上述Pg对ESCC的促进作用。