Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Latrodectus tredecimguttatus (commonly known as black widow spiders) have toxins not only in their venom glands, but also in other parts of their body, in their eggs and even in the newborn spiderlings. The study on the toxins in venom and materials outside the venom glands of the spiders to elucidate their differences and similarities, evolutional relationship and biological functions is of important theoretical and applicable significance. The development of modern protein chemistry and proteomics techniques has provided efficient means for the study of protein and peptide toxins of L. tredecimguttatus. By using such techniques, the molecular base and action mechanism of the toxins can be revealed at the levels of both single purified proteins and omics. Up to now, although protein chemistry and proteomics study on L. tredecimguttatus toxins have achieved a certain progress, the relevant work particularly that on the toxins in the materials outside the venom glands has to be further deepened.
Animals ; Arthropod Proteins ; chemistry ; Black Widow Spider ; chemistry ; Proteomics ; Venoms ; chemistry

Hemopoietic stem cells are mostly obtained from bone marrow,umbilical cord blood and peripheral blood.Physical status of final-stage liver disease patients is poor,and these patients hardly endures large wound.Thus,it is a potential method to harvest hepatocytes following transdifferentiation using peripheral blood stem cell transplantation.It is necessary to mobilize peripheral blood when enough stem cells are needed during peripheral blood stem call transplantation.Stem cell mobilization agent contains 3 types at present:chemotherapeutic drugs,polyanion preparation such as dextran sulphate,various recombinant human hematopoietic cell stimulating factors.For patients with severe liver disease,it is safe to use various recombinant human hematopoietic cell stimulating factors.Density gradient centrifugation is widely used to isolate hematopoietic stem cells.This method can obtain leukocytic cream that is rich of mononuclear cells,and primarily enrich CD34~+ cells.With the discovery of CD34~+ different antigenic determinant monoclonal antibody,immunological technique of exactly,abundantly,rapidly,positively sorting CD34~+ cells is developed quickly,such as solid phase sorting method and flow cytometry sorting.Utilizing the high reproductive activity and multi-directional differentiation,hematopoietic stem cells are amplified in vitro and induced to differentiate into abundantly hemopoietic progenitor cells in a short period,and into directional amplification of various cells such as hepatocytas for transplantation.Presently,bone marrow hematopeietic stem cells can successfully differentiate into hepatocytas.To judge whether differentiated cells were from infused stem cells in transplanted organs,the infused cells were commonly labeled before transplantation.Labeling method includes fluorescent labeling,specific staining,genetic mark and chromosome identification.Subsequently,this was identified using immunological,molecular biological,and fluorescence chemical techniques.Presently,hematopoietic stem cell transplantation in treatment of liver disease is mostly basic experiment,but has not been applied in clinic.

Objective To research the effect of type 2 diabetes on collagen content in different organsinOLETFrats.Methods 24maleOLETFratsweregivenhigh‐caloriedietfor30weekstoset up the T2DM. At the 30th week ,16 male T2DM OLETF rats were selected as case group ,and 12 LETO rats were used as control group. The skin ,liver ,and femur tissue were collected at the same time. HE and Masson staining and immunohistochemical test were performed to measure the content of collagen and HPLC was used to detect hydroxyproline. Results Compared with the NC group ,DM group showed the collagen fibers were decreased in femoral and skin tissue ,but increased in liver tissue. The collagen content of the skin tissue was significantly lower in DM group than in NC group [(149.9 ± 18.4) vs (266.0 ± 54.8)mg/g ,P<0.01] ,and the collagen content of the femur tissue was also lower in DM group than in NC group [(185.60 ± 19.38) vs (243.27 ± 22.19)mg/g ,P<0.05]. In contrary ,the collagen content of liver tissue was higher in DM group than in NC group [(134.54 ± 18.78) vs (64.52 ± 21.51) mg/g ,P<0.01]. Conclusion The effect of type 2 diabetes on the collagen in different organs is different. The collagen content decreases in the femoral and skin tissue but increases in the liver.

With high morbidity and mortality,nosocomial bacterial meningitis(NBM)is a kind of serious nosocomial infection.Post-neurosurgical patients are at a high risk of this infection andNBM in-creases hospital stay and medical expense obviously.In this article,we reviewed recent studiesonpathogen-esis,treatment,and research progress of NBM in neurosurgical patients,and presented unresolved ques-tions and research direction in the future.

Positron emission tomography,a kind of functional imaging technology might reflect tissue metabolism,and had become an important tool in oncologic study.Radiolabelled glucose,amino acid and ligand are commonly used as PET tracer in meningioma diagnosis.Various tracers reproduce different aspects of meningioma metabolism,while meningioma proliferation rate closely related to functional activity.PET could give us information on meningioma biologic characters in vivo atraumatically.As a functional imaging technology,PET had shown incomparable advantage in differential diagnosis of meningioma,and monitoring therapeutic response of meningioma.

Magnetic resonance diffusion imaging contrasts by water molecule diffusion,reflecting water diffusion status.Owing to the unique mechanism,diffusion imaging is sensitive to anatomic alteration of fiber tract and becomes an important technique in the detection of diffuse axonal injury.Diffusion-weighted imaging shows DAI lesion via emphasizing diffusion composition,diffusion tensor imaging is more sensitive to diffusion anisotropy of the lesion,and fiber tractography can directly demonstrate fiber disruption.MR-diffusion imaging not only improves conspicuity of DAI,but also has grading and prognostic value.