Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective:To evaluate the influence of sodium hypochlorite (NaOCl) solution used during root canal therapy on dentin bond strength.Methods:In the study,15 freshly extracted human third molars with complete dental crowns,caries and filling-free were selected.The occlusal enamel was removed perpendicular to the long axis of the tooth to expose middle flat surfaces of sound dentin.The occlusal dentin surfaces were then polished using 600-grit silicon papers for 1 min and rinsed with deionized water for 1 min.The teeth were randomly divided into three groups according to the treatment received:group A (negative control group),the samples were immersed in deionized water for 20 min;group B,the dentin surfaces were immersed in 2.50％ NaOCl solution for 20 min,with the solution being renewed every 5 min;group C,the dentin surfaces were immersed in 5.25％ NaOCl solution for 20 min,with the solution being renewed every 5 min.All the treated dentin surfaces were bonded using a self-etching adhesive system (SE bond) with a 5 mm in height resin composite (AP-X).After storage in deionized water at 37 ℃ for 24 h,the adhesive samples were sectioned longitudinally to produce 1.0 mm ± 1.0 mm stick specimens (n =45) for micro-tensile bond strength testing (MPa).Failure modes (adhesive failure,cohesive failure or mixed failure) at the dentin-resin interface were observed using a stereomicroscope.The micro-tensile bond strength data among the three groups were analyzed by a one-way ANOVA,then the Post-hoc test(LSD) was employed for pairwise comparison.The distribution of failure modes among the groups were analyzed by chi square test.Results:Significant decreased bond strength values were found for the 2.50％ NaOCl-treated group (26.04 ± 5.74) MPa and 5.25％ NaOCl-treated group (24.46 ± 3.77) MPa when compared with the strength of negative control group (48.71 ± 7.77) MPa,P =0.000.Compared with the negative control group,themicro-tensile bond strength of the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated grouphad dropped by 46.5％ and 50.2％.However,there was no significant difference of bond strength between the 2 NaOCl-treated groups (P =0.214).The distribution of failure modes showed significant difference in all the three groups (2 =56.324,P =0.000).The mixed failure (68.9％) was the most mode of fracture in the negative control group,followed by adhesive failure (24.4％),and the cohesive failure was leas t (6.7％).The proportion of adhesive failure mode was higher in NaOCl-treated groups than in negative control group (P =0.000).There was no significant difference of the distribution of failure modes between the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated group(P =0.197),and there was no cohesive failure mode detected in the two groups.Conclusion:The micro-tensile bond strength of dentin to composite resin was lower after exposure to NaOCl solution.

Objective To explore the influence of different anesthetics on emergence agitation (EA) after general anesthesia. Methods 134 EA patients after general anesthesia were randomly divided into three groups. Group A (n=42), Group B (n=45) and Group C (n=47) was given intravenously parecoxib 40 mg, butorphanol 20μg/kg and tramadol 1 mg/kg, respectively. The improvement of agitation, VAS score and Ramsay score were compared and analyzed among the groups. Results After treatment, the degree of EA in Group A, Group B was significantly lower than that in Group C ( <0.05) . VAS score in all the cases was reduced, and Ramsay score was increased compared with that before treatment ( <0.05) . VAS score in Group C was significantly higher than that in Group A, Group B (<0.05) . Ramsay score in Group B was significantly higher than that in Group A, Group C (<0.05) . After treatment, awaking time and postanesthesia care unit (PACU) stay in Group B was significantly higher than that in Group A, Group C (<0.05) .Conclusions Parecoxib is safe and effective for EA after general anesthesia, it can effectively improve the degree of EA, increase postoperative sedative effect, and shorten PACU stay.

Hemopoietic stem cells are mostly obtained from bone marrow,umbilical cord blood and peripheral blood.Physical status of final-stage liver disease patients is poor,and these patients hardly endures large wound.Thus,it is a potential method to harvest hepatocytes following transdifferentiation using peripheral blood stem cell transplantation.It is necessary to mobilize peripheral blood when enough stem cells are needed during peripheral blood stem call transplantation.Stem cell mobilization agent contains 3 types at present:chemotherapeutic drugs,polyanion preparation such as dextran sulphate,various recombinant human hematopoietic cell stimulating factors.For patients with severe liver disease,it is safe to use various recombinant human hematopoietic cell stimulating factors.Density gradient centrifugation is widely used to isolate hematopoietic stem cells.This method can obtain leukocytic cream that is rich of mononuclear cells,and primarily enrich CD34~+ cells.With the discovery of CD34~+ different antigenic determinant monoclonal antibody,immunological technique of exactly,abundantly,rapidly,positively sorting CD34~+ cells is developed quickly,such as solid phase sorting method and flow cytometry sorting.Utilizing the high reproductive activity and multi-directional differentiation,hematopoietic stem cells are amplified in vitro and induced to differentiate into abundantly hemopoietic progenitor cells in a short period,and into directional amplification of various cells such as hepatocytas for transplantation.Presently,bone marrow hematopeietic stem cells can successfully differentiate into hepatocytas.To judge whether differentiated cells were from infused stem cells in transplanted organs,the infused cells were commonly labeled before transplantation.Labeling method includes fluorescent labeling,specific staining,genetic mark and chromosome identification.Subsequently,this was identified using immunological,molecular biological,and fluorescence chemical techniques.Presently,hematopoietic stem cell transplantation in treatment of liver disease is mostly basic experiment,but has not been applied in clinic.

BACKGROUND:Previous studies have shown that erythropoietin can protect neurons and promote nerve regeneration. OBJECTIVE:To explore the therapeutic effect of erythropoietin gene-modified bone marrow mesenchymal stem celltransplantation via caudal vein on rat cerebral infarction. METHODS:Western blot assay was used to identify the expression of exogenous erythropoietin in bone marrow mesenchymal stem cells. A model of middle cerebral artery occlusion was established in Wistar rats using thread method. And then, model rats were randomly divided into model group (PBS injection via the caudal vein), transplantation group (transplantation of bone marrow mesenchymal stem cellsuspension), erythropoietin group (transplantation of erythropoietin-transfected bone marrow mesenchymal stem cellsuspension). Neurologic function was assessed at 3 days, 1, 2, 3, 4 weeks after celltransplantation. Four weeks after transplantation, the rats were decapitated after anesthesia to take brain tissues for RT-PCR detection of Bcl-2/Bax gene expression. cellapoptosis was measured by TUNEL. Hematoxylin-eosin staining and fluorescence microscopy were employed to observe the survival and distribution of PKH26-labeled bone marrow mesenchymal stem cells. RESULTS AND CONCLUSION:Western blot results showed that erythropoietin-transfected bone marrow mesenchymal stem cells could express the erythropoietin in vitro. At 1, 2, 3, 4 weeks after transplantation, the neurological defect scores in the transplantation group and erythropoietin group were significantly lower than those in the model group (P<0.05, P<0.01). The expression of bcl-2 gene in the infarct region was significantly higher in the erythropoietin group than the transplantation and model groups (P<0.05), but the expression of bax was significantly decreased (P<0.05). In the erythropoietin group, the number of apoptotic cells was reduced, and the number of PKH26 positive cells was increased as compared with the other two groups (P<0.05). These findings indicate that the transplantation of erythropoietin-modified bone marrow mesenchymal stem cells via caudal vein can significantly improve the neurological function in the rats with cerebral infarction.

ObjectiveTo investigate the clinical features of intramedullary spinal cord tumors(IMSCT) in children.MethodsThe clinical data including sex,age,pathohistology,clinical manifestations and medical check-up collected from 16 children diagnosed as IMSCT in Shengjing Hospital Affiliated to China Medical University,were retrospectively reviewed and analyzed.ResultsThe study subjects inclu-ded 11 male and 5 female,with an average age of(8.55?4.28) years old,ranged from 15 months to 14 years old.The interval lasted 1 day to 9 years from onset of the syndrome to confirmed diagnosis of IMSCT.The first clinical manifestation was mostly pain.The main clinical mani-festations included limb dyskinesia in 14 cases,pain in 12 cases,urine and stool disturbance in 9 cases,gait irregularity in 8 cases,extremity atrophy in 3 cases.Medical check-up included abnormal tendon reflex in 14 cases,abnormal superficial reflex in 9 cases,sphincter dilatation in 6 cases,positive Babinski's signs in 5 cases,spinal deformity in 4 cases,region masses in 3 cases,paraesthesia in 2 cases,inequality of lower limb in 1 case,dermai sinus in 1 case,pigmentation in 1 case.The cases were evaluated by magnetic resonance imaging,which presented thickened spinal cord and intramedullary abnormal signals in all the cases.Pathological types: teratoma in 5 cases,dermoid cysts in 2 cases,epidermoid cyst in 2 cases.Misdiagnoses included urinary tract infections in 2 cases,rheumatoid arthritis in 1 case,and lumbar disc protrusion in 1 case.ConclusionsThe main pathohistology of pediatric IMSCT is congenital tumor.First clinical manifestation is pain.MRI can show a reliable evidence for the diagnosis.It is important to improve the outcomes of pediatric IMSCT by establishing the diagnosis and administering proper management as early as possible.

AIM:To investigate the effect and safety of modified small incision cataract surgery in patients with soft core cataract.METHODS: Totally 80 cases (109 eyes) of soft cataract patients who underwent surgical treatment from January 2015 to May 2016 in ophthalmology center of our hospital, using random number table method were divided into improved group (improved manipulation of small incision cataract surgery), traditional group (traditional approach to small incision cataract surgery), 40 cases in each group.Comparison of the two groups of treatment, the incidence of complications were taken.RESULTS: There were no significant differences on uncorrected visual acuity between the two groups before and at 1d, 1wk and 1mo after operations (P>0.05).The uncorrected visual acuity of the two groups improved significantly at 1d, 1wk and 1mo after operations compared with those before operations (P<0.05).There were no significant differences on intraocular pressure(IOP) between the two groups before and at 1d, 1wk and 1mo after operations (P>0.05).The IOP of the two groups increased significantly at 1d, 1wk and 1mo after operations compared with those before operations (P<0.05).The complication rate was 9% (5/54) in the modified group, significantly lower than that in the conventional group 25% (14/55) (P<0.05).CONCLUSION: Improved small incision cataract surgery for soft cataract patients was with reliable results, less complications.