This study was aimed to observe the clinical curative effect of Thesium Chinese tablets in the treatment of chronic bronchitis (syndrome of phlegm heat in the lung). A total of 100 cases of chronic bronchitis (syndrome of phlegm heat in the lung) were randomly divided into two groups. The control group was treated with anti-inflamma-tion, relieving cough and asthma, phlegm removing and oxygen inhalation, maintenance of water electrolyte balance and other symptomatic treatment. In the treatment group, on the basis of treatments in the control group, Thesium Chinese tablets were given at the dosage of 1.6 geach time, 3 times a day. Both groups were treated for 1 course of 10 days. The results showed that clinical symptoms and traditional Chinese medicine (TCM) syndromes of quantizing integral were improved after treatment in both groups. And the therapeutic effect of treatment group was significantly better than the control group (P< 0.01). Thesium Chinese tablets can effectively shorten the time of clinical symptom improvement time, obviously improve the level of Ig among elderly patients with acute exacerbation of chronic bron-chitis, and enhance the immune function. It was concluded that the Thesium Chinese tablets can effectively improve the quality of life among patients with chronic bronchitis (syndrome of phlegm heat in the lung).

Objective: To study the changes in radiosensitivity and cell cycle distribution of HeLa cells after inhibition of one or several DNA double-strand break (DSB) repair proteins by small interfering RNA (siRNA) and LY294002. Methods: Ku80 silenced cells (HeLa/Ku8O-siRNA) and control cells (HeLa/ Neg-siRNA) were transfected with siRNA targeting DNA-dependent protein kinase catalytic subunit (DNA-PKcs) or pretreated with 50 μmol/L LY294002, a chemically specific inhibitor of DNA-PKcs. After the cells received 6MV X-ray irradiation, the radiosensitivity of the cells was detected by clony formation assay and cell cycle distribution was analyzed by flow cytometry. Results: The survival fraction at 2 Gy (SF2) value was 0.08 ± 0.01 for HeLa/Ku80-siRNA cells after being transfected with DNA-PKcs siRNA; the SF2 value reached 0.03 ± 0.01 when HeLa/Ku80-siRNA cells were pretreated with LY294002. Both of them were significantly lower than that of untreated HeLa/Ku80-siRNA cells (0.20 ± 0.05). The cells in all the groups were arrested in G2/M phase after irradiaton with 6 Gy X-ray. The G2/M arrest occurred slowly in the DNA -PKcs-siRNA-transfec ted HeLa/Neg-siRNA cells and LY294002-pretreated HeLa/Ku80-siRNA and HeLa/Neg-siRNA cells, which did not reach the peak at 72 h post-irradiation. The G2/M accumulation was maximal at 48 h post-irradiation in other cell lines. Conclusion: Based on 95% inhibition of Ku80 protein, DNA-PKcs or ataxia-telangiectasia mutant (ATM) gene could compensate the DSB repair function. Co-inhibition of these proteins led to increase in radiosensitivity of HeLa cells; Ku80, DNA-PKcs and ATM play different roles in G2/M arrest.

Objective: To explore the correlation between the expressions of DSB (DNA double-strand break) repair protein (including Ku80, DNA-PKcs, and ATM) and radiosensitivity parameters of human tumor cell lines, and to reveal the value of the three proteins for the prognosis of the radiosensitivity of tumor cells. Methods: Eight tumor cell lines were selected including four human cervical carcinoma cell lines (HeLa, SiHa, C33A, and Caski), three human breast carcinoma cell lines (MCF-7, MDA-MB-231, MDA-MB453), and one human lung carcinoma cell line (A549). The expressions of Ku80, DNA-PKcs and ATM protein were measured by Western blotting. The apoptotic ratio of tumor cells was analyzed by flow cytometry after 48 h X-ray irradiation at 10 Gy of 6 MV. SF2 value (survival fraction at 2 Gy) and α and β values were obtained by clone formation assay. The correlation of protein expression with SF2, α/β value or apoptotic ratio was analyzed by Pearson linear correlation analysis. Results: The expression of same protein in different cell lines and the expression of the three proteins in the same cell line had significant difference. There was a positive correlation between the expression of DNA-PKcs and SF2 (r=0.723, P=0.043 <0.05). But Ku80 and ATM expression had no correlation with SF2 (P>0.05). The expression of the three proteins had no correlation with either apoptotic ratio or α/ β value (P>0.05). Conclusions: Tumor cells with higher expression of DNA-PKcs protein will have higher radioresistance. The expression level of DNA-PKcs protein in tumor cells may be an indicator for predicting the radiosensitivity of tumor cells.

Objective:To evaluate the value of serum anti citrullinated antibody ( anti CCP-Ab) ,anti keratin antibody ( AKA) and rheumatoid factor ( RF) diagnostic significance of these 3 kinds of index in RA.Methods:256 patients with RA ,92 patients with other autoimmune diseases,50 cases of healthy people were anti CCP-Ab,AKA,RF detection,and the results were analyzed.Results:single target detection ,diagnostic sensitivity of RA from high to low was RF ,anti CCP-Ab,AKA,specific from high to low was AKA ,anti CCP-Ab, RF,anti CCP-Ab and AKA combined detection in clinical diagnostic efficiency.Conclusion: RF,AKA,anti CCP-Ab three index have advantages and disadvantages in diagnosis of RA ,the diagnosis of AKA ,anti CCP-Ab serum of RF negative RA is a powerful complement to,in accordance with the selection of project portfolio appropriate experimental conditions ,the early diagnosis of RA and early treatment ,improve the prognosis had higher value.

Objective:To establish the quality control for Yangxueyin oral liquids. Methods:TLC was applied to identify Angeli-cae Sinensis Radix and Fructus Ziziphi Jujubae. The content of astragaloside A in Yangxueyin oral liquids was determined by HPLC-ELSD. A Luna C18(250 mm ×4.6 mm,5 μm)column was used, the mobile phase was acetonitrile-water (38∶62)with the flow rate of 0. 8 ml·min-1 , and the column temperature was 40℃. The temperature of the drift tube was 85℃, and the flow rate of the carrier air was 2.0 L·min-1. Results: The linear range of astragaloside A was 0.522-4.176 μg(r =0.999 8). The average recovery was 97. 9% with RSD of 1. 03% (n=6). Conclusion:The method is convenient, sensitive and accurate, which can be used in the quality control of Yangxueyin oral liquids.

Objective　To study the causes of failure to IOL implantation during cataract surgery.Methods　We reviewed 540 eyes in which 23 eyes were failed to implant IOL during cataract surgery.Results　Detachment of suspended zonular ligament, rupture of posterior capsule and vitreous prolapse are the main factors influencing IOL implantation during cataract surgery.Conclusion　Improvement of surgical technique and reduction of complications are the key points for the IOL implantation successfully during cataract surgery.

Objective To investigate the variance of peripheral blood prolactin(PRL)in controlled ovarian stimulation.Methods Seventy-two patients,with totally 106 cycles receiving a long protocol of gonadotropin-releasing hormone agonist combined with gonadotropin(Gn)were randomly enrolled in this retrospective study.During controlled ovarian stimulation,peripheral blood hormones were measured by chemiluminescent microparticle immunoassay.Results Prolactin was positively correlated with estradiol (r=0.5897.P＜0.01)while there was no significant correlation between luteinizing hormone and PRLProgesterone had a positive relation with prolactin(r=0.1412,P＜0.01).Conclusions During controlled ovarian stimulation,prolactin secretion is not affected by Gn but may be stimulated by estradiol.Progesterone has a positive relation with prolactin.

Objective To investigate effects of mental nursing on psychological state of patients with pneumoconiosis. Methods 160 pneumoconiosis patients were divided into 8 groups randomly,and 20 patients were included in each group. Psychological characteristics were observed before mental nurs-ing, and 15, 30 and 60 days after mental nursing. The numbers of the youth, the middle-aged and the el-derly patients who demonsrated four or more kinds of psychological characteristics after 60 days mental nursing were compared.320 patients of the same kind were divided into the two aspects of mental nursing group and all aspects of mental nursing group, the number of patients who appeared four or more kinds of psychological characteristics after 60 days mental nursing was recorded. Results The number of pneu-moconiosis patients who clearly appeared four or more kinds of psychological characteristics decreased after 15 days, but it had no statistical significance, the number decreased significantly after 30 days and 60 days of mental nursing, and the latter decreased more significantly.Both of the numbers of two aspects of mental nursing group and all aspects of mental nursing group decreased significantly, and the latter decreased more significantly.The number of the youth decreased most significantly, while it had no statistical significance between the middle-aged and the elderly group. Conclusions Mental nursing for the pneumoconiosis pa-tients is a long-term project, comprehensive mental nursing to pneumoconiosis patients has great influence, but the influence varies for patients with different ages.

Adult ; Aged ; Antineoplastic Agents ; therapeutic use ; Benzamides ; Chemotherapy, Adjuvant ; Female ; Follow-Up Studies ; Gastrointestinal Neoplasms ; drug therapy ; surgery ; Gastrointestinal Stromal Tumors ; drug therapy ; secondary ; surgery ; Humans ; Imatinib Mesylate ; Liver Neoplasms ; secondary ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Piperazines ; therapeutic use ; Pyrimidines ; therapeutic use

Objective To evaluate the levels of neutrophil gelatinase associated lipocalin (NGAL) in prediction of acute kidney injury (AKI) in children with burn. Methods Forty seven children with burn were selected in this study and thirty healthy children were enrolled as a control group. Levels of blood routine, urine routine, serum creatinine (Scr), serum NGAL (SNGAL), urine NGAL (UNGAL), blood urea nitrogen (BUN) and C-reactive protein (CRP) were measured. Receiver operating characteristic (ROC) curve were used to estimate the AKI diagnostic value of serum NGAL, urine NGAL and Scr. Results On the day of admission, serum NGAL and urine NGAL were significantly increased in patients with AKI , while Scr did not change significantly as compared with those in non-AKI patients and the healthy controls. In AKI group, serum NGAL and urine NGAL were positively correlated with WBC and CRP on the day of admission , and positively related with Scr on the seventh day. ROC analysis showed the AUC of serum NGAL, urine NGAL and Scr on admission were 0.737(95%CI: 0.653 ~ 0.779, P < 0.05), 0.772(95%CI: 0.632 ~ 0.793, P < 0.05), and 0.508(95%CI: 0.454~0.618, P < 0.05) respectively. The cut-off value of serum NGAL and urine NGAL were 318 μg/L (sensitivity of 72.8% and specificity of 81.6%) and 105 μg/L (sensitivity of 75.6% and specificity of 83.7%). Conclusions Elevation in levels of serum NGAL and urine NGAL occurs earlier than that in Scr level in children with burn. Both serum and urine NGAL level can be used as a biomarker for early prediction of AKI.