Acute intermittent visible fluorescent light was used as an experimental light source (18175?300lux, 1 hour dark: 1 hour light, 3 cycles). After light exposure, the myeloperoxidase activity (MDA) levels increased in rat retina, and the retinal structure and blood-retinal barrier were damaged. When 2 doses of anisodaminum (10 mg/Kg) were given 12 hours and just before light exposure, MDA levels lowered and the retinal structure was protected.

The injectable calcium phosphate material is present to solve the clinical bone defects of bone tissue and the potential to undermine the ideal substitute. But the properties and biological evaluation are still to be improved. Therefore,the material of the academic community and the academic orthopedic bone cement was modified for a variety of studies,and the addition of strontium bone cement significantly improved the performance. The article indicated strontium-mixed calcium phosphate bone cement was characterizing by the compressive strength,the degradability,the hardening time,the solubility,the injection and the anti-water-solubility,suggesting the clinical practice of the strontium-mixed calcium phosphate bone cement. Prospectively,the strontium-mixed calcium phosphate bone cement may take in the bone tissue repair domain as a artificial bone substitution material.

Objective To assess the safety and feasibility of minimally invasive percutaneous nephrolithotomy(mPCNL) in the treatment of solitary kidney calculi.Method Thirty-eight patients with solitary kidney calculi,treated with mPCNL from Jan 1998 to May 2004,were retrospectively investigated.Results Of 38 cases,there was 6 cases with pyonephrosis and 32 cases with the treatment of one stage percutaneous nephrolithotomy.With the treatment of 1 to 3 tract was used for calculus removed in all cases,a total of 86% stone-free rate was achieved.Only one case with pyonephrosis suffered from postoperative infection shock while one stage minimally invasive percutaneous nephrolithotomy was performed.Another one patient with diabetes suffered from postoperative ketoacidosis and no major complications were noted in other patients.Conclusion Minimally invasive percutaneous nephrolithotomy is safe and effective in the treatment of solitary kidney calculi.

Objective To establish an evaluation index system for access to ophthalmic surgery so as to standardize the access. Method Collect 14 ophthalmologists' recommendations on access criteria for ophthalmic surgery in two rounds of questionnaire survey by Delphi method. Results The return rate of rounds of consultation questionnaire was 93.33%, and the average expert authority coefficient was 0.78.Nine ophthalmic surgery access indicators were established. The coordination of expert coefficient was 0.521 after two consultation. It had significant difference ( P＜0.01 ). Conclusion The ophthalmic surgery access indicator system is highly credible and allows quantitative assessment of ophthalmic surgery from various aspects. It is more demanding for ophthalmologist, and gets more involvement of patients in the evaluation of ophthalmic surgery access. It could contribute to scientific and rational decision-making on access for ophthalmic surgery.

Objective To investigate,the significance of serum high-sensitivity C-reactive protein (hs-CRP) concentration in subjects with various glucose tolerance and study the role of hs-CRP in patients of impaired glucose tolerance (IGT) and diabetes mellitus.Method All subjects in the study underwent oral glucose tolerance test (OGTT).Determination of serum hs-CRP levels in normal glucose tolerance(NGT),IGT and type 2 diabetes mellitus (T2DM) patients were made by immunologic projective turbidimetry method.Results The levels of serum hs-CRP were (1.57 + 0.46) mg/L in NGT patients,(2.84 ±0.48) mg/L in IGT patients,and(4.18± 0.76) mg/L in T2DM patients.The levels of serum hs-CRP were positively correlated with body mass index,systolic blood pressure,diastolic blood pressure,fasting plasma glucose,OGTr 2 hPG,triglyceride,and total cholesterol.ConclusionsInflammation has already existed not only in T2DM patients but also in subjects with IGT.Inflammation might participate in the oeeurrance and development of T2DM.hs-CRP as an inflammator factor can be used to predict and monitor T2DM.

Objective To investigate the distribution and drug resistance of pathogenic bacteria in children with blood stream in-fection(BSI) in Beihai area .Methods The clinical data of children with blood stream infection from January 2013 to June 2016 were retrospectively analyzed .Results 95 .3% of BSI children patients were community acquired infection .The proportions of Gram-pos-itive cocci and Gram-negative bacilli were 53 .5% and 46 .5% respectively .The resistance rates of vancomycin and linezolid to Gram-positive cocci all were 0% ;which of piperacillin ,piperacillin/tazobactam ,cefepime and meropenem to Gram-negative bacilli were 9 .3% ,0 .0% ,9 .1% and 5 .0% respectively .Conclusion The proportion of Gram-positive cocci and Gram-negative bacilli is basical-ly consistent .Vancomycin and linezolid can be used as the empiric medication of Gram-positive cocci BSI ;piperacillin ,piperacillin/tazobactam ,cefepime and meropenem and can be used as the empiric medication of Gram-negative bacilli BSI .

This paper aimed to analyse and review the literature of diagnosis and treatment of Chinese aphasia at home in recent years. The advanced brain imaging technology and events related potentials, besides the traditional neuropsychological check method, had been much more applicated and studied in the diagnostic research of Chinese aphasia at present, and in the future the check methods of Chinese aphasia showed diversification, systematic and standardized development. A large number of clinical reshearches tended to comprehensive treatment as the main approach for Chinese aphasia in a conclusion.

ObjectiveTo investigate the effects of norepinephrine on oxygen metabolism and postoperative outcomes in the elderly patients undergoing fluid-restored abdominal surgery.MethodsAfter hospital ethics committee approval,and written informed consent was obtained from all patients,40 elderly patients,aged ＞ 64 yr,with a body mass index of 18-24 kg/m2,undergoing elective colorectal surgery,were randomly divided into 2 groups (n =20 each):routine fluid administration group (group A) and restricted fluid administration group (group B).In group A,lactated Ringer's solution was given routinely.Lactated Ringer's solution was given at a rate of 5 ml· kg- 1 · h- 1 and small dose of norepinephrine was infused intravenously at 0.01-0.03 μg· kg- 1 · h - 1 simultaneously during the surgery,and MAP was maintained ≥65 mm Hg in group B.Intraoperative blood loss was replaced with the equal volume of 6％ hydroxyethyl starch 130/0.4.Before the surgery ( baseline),1 and 2 h after beginning of the surgery,and while leaving postanesthesia care unit,mean arterial pressure (MAP),heart rate (HR),cardiac index (CI) and central venous pressure (CVP) were recorded,and arterial and central venous blood samples were drawn for blood gas analysis.Oxygen delivery index (DO2 I ),oxygen consumption index (YO2I) and oxygen extraction ratio (ERO2) were calculated.The gastric mucosal pH was determined before the surgery and 1 h after beginning of the surgery.Blood samples were taken form the peripheral vein to determine the concentration of Hb,serum albumin,blood urea nitrogen (BUN) and creatinine (Cr) concentrations before the surgery and 1 day after the surgery.The extubation time,duration of stay in hospital,the time when the patients passed the flatus,pulmonary complications,gastrointestinal complications,and wound infections were recorded.ResultsCompared with group A,CI,Hb,ScvO2 and DO2I were significantly increased,CVP and ERO2 were decreased during the surgery,the concentrations of Hb and serum albumin were significantly increased,1 day after the surgery,and the incidence of pulmonary complications and wound infections was significantly decreased after the surgery in group B (P ＜ 0.05 ).There were no significant differences in gastric muscosal pH,the indexes of renal function,extubatiotn time,the time when the patients passed the flatus,duration of stay in hospital and the incidence of gastrointestinal complications between the two groups ( P ＞ 0.05).ConclusionWhen small dose of norepinephrine is used to treat perioperative hypotension induced by fluid restriction,it can increase oxygen delivery,and reduce the incidence of postoperative complications,and has no adverse effects on gastrointestinal microcirculation and oxygen consumption in the elderly patients undergoing colorectal surgery.

Objective To study the lung protection of ambroxol in general thoracic surgery perioperation for the elderly.Methods Totally 130 hospitalized patients aged (65.1±4.3) years in our hospital during general thoracic surgery were randomly divided into two groups:ambroxol intervention(2 mg/kg,intravenous drip,once/d) and control groups (n=65 for each).The incidenceof pulmonary complications,changes of blood gas analysis before and after surgery and time of hospitalization were compared between the groups.Results The incidence of pulmonary complications was significantly lower in ambroxol intervention group than in control group (9.23％ vs.24.62％,P＜0.05).Blood gas analysis results before and after surgery were improved in ambroxol intervention group[PaO2:(61.32±8.65) mm Hg,(95.07±13.62)mm Hg; PaCO2:(44.15±5.52)mm Hg,(37.28±4.06)mm Hg] as compared with control group[PaO2:(62.11 ± 8.83) mm Hg,(80.37±10.46)mm Hg; PaCO2:(44.39±5.17) mm Hg,(40.73±4.51)mm Hg] (t=3.61,3.75,both P＜0.05).The time of hospitalization was shorter in ambroxol intervention group than in control group [(12.14±0.29) d vs.(15.85±0.62)d,t=3.41,P=0.036].Conclusions Perioperative use of ambroxol in general thoracic surgery in the elderly is worthy of clinical application because of reduction of pulmonary complications and shortness of hospital stay.

Objective To investigate the clinical efficacy and its security on different kinds of atomization with mucosolvan and chymotrypsin in treating neonatal pneumonia.Methods Seventy-eight neonates who were diagnosed as neonatal pneumonia were divided into treatment group (n =39) and control group (n =39) by random number table method from Jul 2011 to Aug 2013 in our hospital.The control group was treated with chymotrypsin atomization,and the treatment group was treated with compression atomizing to inhale mucosolvan.The treatment effects of two groups were compared.Results Compared with the control group after treatment for 24 h,48 h,72 h,the treatment group had more significant increasing in PaO2,more decreasing in PaCO2 and more significant improvement in oxygenation index.There were statistical significances between the two groups(P ＜ 0.01).The treatment group spent shorter time in remission of symptoms,disappear of signs and hospital stay than that of the control group(P ＜ 0.05).In the treatment group,25 cases were markedly improved,9 cases were effective,5 cases were invalid.The effective rate was 87.2％.In the control group,14 cases were markedly effective,6 cases were effective,19 cases were invalid.The effective rate was 51.3％.The effective rate was significant difference between the two groups (P ＜ 0.01).The adverse drug reactions were not found in the process of treatment for two groups.Conclusion Mucosolvan atomizing inhalation has a better treatment effect than chymotrypsin.It can shorten the course of treatment,and is worth promoting the application.