China ; Communicable Disease Control ; Disease Notification ; Humans ; Population Surveillance

This paper introduces a new method which can judge the degree of burn scar hypertrophy by analyzing chroma of the burn scar. Its technical schedule is as follows: Firstly, the image of the burn scar is captured by using a digital camera. Then the chroma emendation is performed by using an Artificial Neural Network(ANN). At last, the chroma of burn scar is analyzed and the classification of burn scar hypertrophy is given by using a Support Vector Machine(SVM). Compared with clinical evaluation, the result deduced from this method is proved to be effective.

Objective To investigate the relationship between metalloproteinase-9-9 (Matrix),inhibitor-1-1 (MMP-9),metalloproteinase TIMP-1 factor (Matrix) and endothelial growth (VEGF),and to investigate the correlation between the expression of (matrix) and vascular endothelial growth factor (endothelial) and vascular endothelial growth factor (vascular).Methods Sixty-eight cases of non small cell lung cancer patients treated in fengning manchu autonomous county hospital from January to January 2014 were analyzed retrospectively,and the expression level of TIMP-1,VEGF and MMP-9 were analyzed by immunohistochemical method.Results The positive rates of MMP-9 (75％),TIMP-1 (19.12％) and VEGF (13.24％) were significantly higher than those in normal tissues (P＜0.05),MMP-9 (60.87％),VEGF (54.55％),TIMP-1 (65.22％) and VEGF (76.09％,59.09％,63.64％).The positive rates ofTIMP-1,VEGF and MMP-9 were higher than those without lymph node metastasis (P ＜0.05) Conclusion MMP-9,TIMP-1 and VEGF expression levels can predict the invasion and metastasis of non small cell lung cancer,and the three kinds of substances can promote the development and progression of lung cancer in different degrees.

Objective To examine the characteristics of dysfunctional attitudes in patients with so?matoform disorders and to delineate the relationship between dysfunctional attitudes and personality disorder tendency. Methods Sixty?four outpatients with somatoform disorder and 52 healthy individuals were recrui?ted and asked to complete the Personality Diagnostic Questionnaire( PDQ?4) and the Dysfunctional Attitudes Scale( DAS ) for the evaluation of their personality disorder tendency and cognitive characteristics. DAS scores were compared between patient and control groups. The relationship between DAS and PDQ?4 was e?valuated by comparing DAS scores of patients with and without personality disorder tendency and by correla?ting patients’ total scores of DAS and PDQ?4. Results Patients with somatoform disorder(150.13±31.75) showed significantly higher scores of DAS than healthy controls(121.62±20.30). Moreover,patients with per?sonality disorder tendency exhibited significantly higher scores in the total score of DAS and scores of five factors including vulnerability, absorption/exclusive, compulsion, dependence and cognitive philosophy ( P<0.05) . Finally,a positive correlation was found between total scores of DAS and PDQ?4( r=0.535, P<0.01) and the correlation coefficients between the total score of DAS and Cluster A( r=0.545) and C scores( r=0.625) were significantly higher than that of Cluster B score( r=0.317).Conclusion Patients with somato?form disorders have cognitive distortion,which is correlated with personality disorder tendency.

Objective To analyse the tongue characteristics of cancer patients during chemotherapy ,in order to guide the TCM treatment. Methods 42 patients' tongue figure was collected respectively before and after chemotherapy by Canon digital camera, then to analyse the characteristics. Results Purple tongue was the most common in tongue color, account for 38.10%;meanwhile the tongue with stasis patch or with toothmark were common in tongue shape, account for respectively 26.19% and 21.43%;white thick or greasy tongue fur was the most common, especially in the central of tongue. Conclusion Deficience of Qi and blood stasis syndrome is common in cancer patients after chemotherapy, it is helpful to provide tasis for treatment of tonifying Qi and activating blood during chemotherapy.

Acute Disease ; Adult ; Humans ; Hydrogen Sulfide ; poisoning ; Male ; Poisoning ; pathology

Objective To investigate the clinical characteristics, diagnosis and treatment of acute intestinal perforation in the patients with colorectal carcinoma. Methods The clinical data of 38 colorectal carcinomas complicated with acute intestinal perforation treated in our hospital during 19 years were analyzed retrospectively. Results Acute intestinal perforation was common in old patients with colorectal carcinoma (mean age 62 years).The primary lesions were mostly located in left-side colon and superior rectum(27/38,71.1%). The perforation in the proximal colon of tumor and cecum accounted for 81.6%(31/38), and in tumor site accounted for 18.4%(7/38). The correct rate of preoperative diagnosis was only 15.8%(6/38). The postoperative complications were common and severe. The perioperative mortality was 42.1%(16/38). All the causes of death were multiple organ function deficiency. There were close relations between perioperative mortality and operation time. The postoperative recurrent rate was 41.2%(7/17). Five-year survival rate was 23.5%(4/17). Conclusion Early diagnosis, early operation and active prevention and treatment of multiple organ function deficiency were key to decrease the mortality of the colorectal carcinoma patients with acute intestinal perforation.

BACKGROUND:Diabetic foot ulcers threaten the patients’ health and even survival seriously. It is an international difficult problem and lacks an effective treatment. But gene therapy and stem celltherapy possess special advantages and potential in wound healing. OBJECTIVE:To assess the therapeutic effect of transplantation of bone marrow mesenchymal stem cells transfected by human vascular endothelial growth factor 165 (hVEGF165) gene on foot wound healing in diabetic rats. METHODS:Recombinant adenovirus was established in vitro which expressed hVEGF165 gene and transfected into the third generation of bone marrow mesenchymal stem cells. Total y 120 male Wistar rats were divided into five groups:group A (non-diabetic controls), group B (diabetic controls), group C (Ad-hVEGF165 therapy), group D (stem celltherapy) and group E (transplantation of bone marrow mesenchymal stem cells transfected by Ad-hVEGF165 gene). Rats in the latter four groups were intraperitoneal y injected with streptozotocin to induce diabetic models. In al rats, a 3 mm×7 mm rectangular ful-thickness skin sample was cut from the instep of the hind foot to make a model of foot wound. The rats were subcutaneously injected at equidistant six points 5 mm distal to the wound edge on the dorsum of the foot:50μL PBS per point for group A, 50μL adenovirus suspension (1×1013 pfu/L) per point for group C, 50μL stem cellsuspension (1×1010/L) per point for group D, and 50μL adenovirus suspension+50μL stem cellsuspension per point for group E. RESULTS AND CONCLUSION:After injection, the rate of wound healing, the expression of VEGF and the qualities of capil aries in group E were higher when compared with groups B, C, D (P<0.05), but were lower than those in group A (P<0.05). Transplantation of bone marrow mesenchymal stem cells transfected by hVEGF165 gene can promote foot wound healing, angiogenesis and expression of VEGF in diabetic rats.

BACKGROUND:The treatment of limbs’ open fracture with severe soft tissue injury is very complicated. There are many wound exudates, high frequency of dressing change, and high infection rate. How to deal with the contradiction between wound treatment and fracture fixation is the considerable problem in clinical therapy. ＜br> OBJECTIVE:To explore the effect of external fixator combined with vacuum sealing drainage on limbs’ open fracture with severe soft tissue injuries. ＜br> METHODS:A total of 34 patients with limbs’ open fracture with severe soft tissue injuries were enrol ed in the First Affiliated Hospital, Xinxiang Medical Col ege from December 2009 to December 2010. There were 20 males and 14 females, with an average age of 36.6 years old. There were 10 cases of upper limb fracture and 24 cases of lower limb fracture. After debridement, cutaneous deficiency accounted for 1%to 6%of surface area. On the basis of external fixation, the wound received vacuum sealing drainage (treatment group;n=17) and conventional dressing (control group;n=17). According to limb swel ing and granulation growth, delayed suture, skin graft or flap to cover the wound were used. After treatment, infection rate of the wound and growth of granulation were observed. In accordance with wound healing and growth of granulation, curative effects were evaluated. ＜br> RESULTS AND CONCLUSION:In the treatment group, there were 11 cases of healing, 5 cases of effective effects, and 1 case of ineffective effect, with a total effective rate of 94%and the infection rate of about 15%. In the control group, there were 7 cases of healing, 6 cases of effective effects, and 4 cases of ineffective effects, with a total effective rate of 76%and the infection rate of about 40%. Significant differences in healing rate, total effective rate and infection rate were detected between the two groups (P＜0.05). The healing time of wound was 8.66-16.23 days in the treatment group and 15.68-22.36 days in the control group. The healing time was significantly shorter in the treatment group than in the control group (P＜0.05). It is thus clear that vacuum sealing drainage has obvious advantages in repair of limbs’ open fracture with severe soft tissue injury compared with the conventional dressing.

Objective To study the synergistic antitumor effects of quercetin and cisplatin in human prostate cancer PC3 cells and LNCaP cells.Methods Twelve h after PC3 cells or LNCaP cells were seeded,different dose of quercetin (10 μmol/L,20 μmol/L,40 μmol/L,80 μmol/L,160 μmol/L) or cisplatin (0.01 μmol/L,0.05 μmol/L,0.1 μmol/L,1 μmol/L,10 μmol/L),or quercetin (20 μmol/L) + cisplatin (0.01 μmol/L,0.05 μmol/L,0.1 μmol/L,1 μmol/L,10 μmol/L) were added for48 h and then the antiproliferative effects were detected with MTT assay.After incubated with quercetin (20 μmol/L) or cisplatin (0.05 μ mol/L),or quercetin (20 μ mol/L) + cisplatin (0.05 μmol/L) for48 h,cell cycle distribution and apoptosis of PC3 cells or LNCaP cells were detected by flow cytometer,PI and Annexin V staining.Protein expression was detected by Western blotting.Results After treatment with quercetin or cisplatin alone,the IC50 were (0.99 ± 0.13) μmol/L,(0.75 ± 0.09) μmol/L and (91.60 ± 6.10) μ mol/L,(72.90±4.70) μ mol/L for LNCaP cells or PC3 cells,respectively;The IC50 were (0.11±0.06)μ mol/L,(0.07±0.02) μmol/L for quercetin + cisplatin treatment (Compared with quercetin,P＜0.01 ;Compared with cisplatin,P＜0.05.After treatment with cisplatin or quercetin + cisplatin for 48 h,the S phase percent of LNCaP cells or PC3 cells were (22.4±2.7)％,(31.2±2.4)％ and (20.1±1.6)％,(31.0±2.5)％,respectively,(Compared with control,P＜0.05,however,treatment with quercetin alone has no significant difference (Compared with control,P＞0.05).After treatment with cisplatin or quercetin + cisplatin for 48 h,the apoptotic percent of LNCaP cells or PC3 cells were (14.8 ± 1.9) ％,(39.6 ± 3.1) ％ and (11.5± 1.2) ％,(34.1 ±3.3) ％,respectively,(compared with control,P ＜ 0.05,however,treatment with quercetin alone had no significant difference (compared with control,P＞0.05).After treatment with quercetin alone for 48 h,the activation of caspase-3,caspase-8 and caspase-9 were slightly increased,the expressions of Bax and p21 were up-regulated,the expressions of Bcl-2 and CDK2 were down-regulated.Furthermore,these effect of cisplatin and quercetin + cisplatin were significantly enhanced (compared with quercetin,P＜0.05;compared with quercetin,P＜0.01,respectively.Conclusions The combination modality with quercetin and cisplatin has a better treatment effect in vitro not only in androgen-dependent LNCaP cells but also in androgen-independent PC3 cells.