Obesity has become a social problem.Study shows that fat is closely related with chronic diseases.and the effect of body fat on body metabolism varies with its distribution.The discovery of fat as an endocrine organ provides a broad research areas for the relationship between body fat and chronic diseases.The difierense in fat distribution between different sexes suggests that gonadal hormone has obvious effects on body fat metabolism.As a result,hormone replacement therapy has become the optimal method for releasing climacteric symptom.With the development of people's living standards,the attention paid to the menopausal problem,the research on body fat changes and its relationship with chronic diseases,and the study on the effect of hormone replacement therapy on the body fat are of great importance.

AIM: To study rat astrocyte proliferation in ipsilateral hippocampus following focal cerebral ischemia. METHODS: Ischemia was induced by temporary middle cerebral artery occlusion (MCAO). In hippocampus of rats at 3, 7 and 30 days after MCAO, the numbers and anatomic distribution of glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. The protein expression of GFAP and proliferating cell nuclear antigen (PCNA) in the ipsilateral hippocampus were analyzed by Western blot analysis. RESULTS: Astrocytes appeared hypertrophic, with increased process thickness and numbers at 7 days after MCAO, and the highest density of astrocytes were seen at 30 days in the CA1, CA2 regions of the ipsilateral hippocampus. Western blot analysis revealed that GFAP levels were normal at 3 days, but increased by 7 days and remained elevation at 30 days. Western blot analysis of PCNA protein also revealed identified upregulation PCNA at 3 days after MCAO and the expression peaked at 7 days. CONCLUSION: This study demonstrates that focal cerebral ischemia in the rat results in a rapid response, a process often referred to as reactive astrogliosis or glial scarring, from resident astrocytes of the ipsilateral hippocampus to the side of ischemia. [

Protein kinase CK2 is a ubiquitously expressed protein serine/threonine kinase present in all eukaryotes. It can intervene transversally into a multitude of signal pathways.It plays a key role in regulation of cell growth and apoptosis in a hierarchical and vertical manner. It was demonstrated that modest deregulation in the CK2 expression impacts a potent oncogenic potential to the cells.Moreover,inhibition of CK2 with specific chemical inhibitors or CK2-targeting antisense RNA may result in depression of tumor. Thus, studying the regulation mechanism of CK2 in tumorigenesis and developing new anti-cancer drugs by employing specific strategies against CK2 are of significant clinical value.

Objective To investigate the protective effect of Yulangsan polysacharide ( YLSPS) and mechanism against ibuprofen-induced liver injury in mice. Methods The mice were randomly divided into the blank control(NC), the model control,YLSPS at 150 mg·kg-1 , 300 mg·kg-1 ,600 mg·kg-1 groups and biphenyldicarboxylate (150 mg·kg-1 BPDC) group. The mice were orally administered with corresponding agents once per day for consecutive 14 days, whereas the blank control group and model control group were orally administered with saline. Except the blank control group, all the other mice were orally administered IBU 200 mg·kg-1 body weight 2 h after last lavagedof medicimes. The mice were fasted and watered ad lib for 20 h after model establishment. Activities of ALT,AST and ALP,content of T-BiL,TNF-α,IL-6 in serum;activities of SOD,GSH-Px and content of MDA in liver tissue were detected. The morphological pathology test was used to examine degrees of hepatic injury. Results Compared with the model control, YLSPS could obviously reduce activities of ALT,AST and ALP,content of T-BiL, MDA,TNF-α and IL-6, and increase SOD,GSH-Px and CAT (P<0. 05), and then lessen the hepatic injury. Conclusion YLSPS showed potential protective effect against ibuprofen-induced liver injury in mice, the mechanism may be related to attenuating free radical injury and inhibiting lipid peroxidation and lowering release of inflammatory factors.

Objective To assess the prevalence of the metabolic syndrome (MS) in Shanghai adults according to the criteria of the US National Cholesterol Education Programme Adult Treatment PanelⅢ ( NCEP ATP Ⅲ) definition. Methods Randomly selected adults were studied by means of stratified sampling. A cross-sectional study was conducted in Shanghai with a representative sample of 7 414 Chinese adults aged 35-74 years.Results Defined by the ATPⅢ criterion, the preliminary prevalence of MS was 19.81%. The prevalence of MS was 16.21% (95%CI 15.37%-17.05% ) after age was adjusted. The prevalence of MS was higher in female than in male (22.59% vs 16.64% ,P<0.01 ), and higher in urban than rural residents( 17.43% vs 17.16% ,P<0.01 ). The prevalence of MS increased with age in both male and female, especially in females aged above 55 years. There were 19.81% subjects with over 3 risk factors. The most common combination of four components in MS were central obesity, high triglyceridemia, low high-density lipoprotein-cholesterol and hypertension. Conclusions There is a high prevalence of MS in Shanghai adults. MS is increasingly becoming a noteworthy health problem. Early-diagnosis and integrated-methods of prevention and treatment of MS are mandatory.

Objective To study the causes and nursing points of re-operation after orthotopic liver transplantation (OLT). Methods The clinical data of 241 patients who underwent OLT in the past three years were analyzed, and the causes and nursing of re-operation were summarized. Results In the 241 patients,30 cases underwent re-operation. The incidence of re-operation was 12.4%. The causes included: intra-abdominal bleeding in 14 cases with the incidence of 46.7%, biliary complications in 8 cases with the incidence of 26.6%, 4 cases infection in different organs with the incidence of 13.3%, wound dehiscence in 2 cases with the incidence of 6.7% and recurrence of carcinoma in 2 cases with the incidence of 6.7%. 7 patients died after re-operation with the mortality rate of 13.3%. Conclusions The causes of re-operation after OLT were various, intra-abdominal bleeding and biliary complications were the main factors. Knowing the causes and taking rational nursing helped to raise the survival rate and quality of life after OLT.

Objective To acknowledge the quality of life (QOL) in patients after lung transplantation and to explore related factors. Methods A cross- sectional study design and a convenience sampling were performed in this research. Totally 30 patients after lung transplantation were investigated. The questionnaires which used to explore the quality of life were Short Form 36 Health Survey Questionnaires (SF-36) and Revised Airways Questionnaires 20 (AQ20-R). Related state were surveyed by questionnaires consisted of demographic questionnaires, Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS) and Medical Coping Mode Questionnaires (MCMQ). Statistical analysis was performed by SPSS 20.0 software. Correlation analysis and multivariate analysis were performed using Pearson linear correlation analysis and multi-linear regression. Results The scores among the 8 dimensions of SF-36 were(34.48±16.73)-( 71.63±22.83), lower than those of norms(Z=-9.684--2.817, P<0.05 or 0.01). Somatic pain scored the highest (71.63±22.83), while physiological function scored the lowest (34.48±16.73). The mean score of AQ20-R was 7.93±5.21. The major two problems that manifested QOL were: uncomfortable feeling of lung caused by strong scent, smog or perfume, exhausted feeling after having a cold. The mean scores of SAS and SDS were 44.33±9.33,48.05±9.80, higher than those of norms, which were 33.80 ± 5.90, 41.88 ± 10.57 (t=6.1833, 3.4458, P < 0.01). The scores among the 3 dimensions of MCMQ were 8.93 ± 2.08, 16.10 ± 2.28, 3.63 ± 1.33 and the differences were significant in 3 dimensions compared with those norms, which were 19.48±3.81, 14.44±2.97, 8.81±3.17(t=-27.7281, 3.9885,-21.3878, P<0.01). Conclusions In SF-36, role-physical of patients after lung transplantation was the worst dimension, while bodily pain was the best dimension of QOL. The QOL of patients after lung transplantation were almost worse than normal people. The related factors of QOL in patients after lung transplantation maybe:anxiety, depression and medical coping style.

Objective To assess the therapeutic effect of music- regulated laser therapy on mild and moderate primary hypertension (PH) so as to provide clinical evidence for the development of an effective non-drug therapy for hypertension. Methods One hundred mild and moderate PH patients were divided into obser-vation ( n = 50 ) and control groups ( n = 50 ) according to their stage of hypertension. There were two phases of treatment with 6 weeks in each phase for a total of 12 weeks. In the first phase, patients in the observation group received music-regulated laser therapy while the control group received drug therapy. In the second phase, the treatments were reversed, with the observation group receiving the drugs. The blood pressure and quality of life (QOL) of all the patients was evaluated after each phase. Results Before therapy there were no significant differences in average blood pressure or QOL between the groups. After the first phase, systolic blood pressure (SBP) and diastolic blood pressure (DBP) had been reduced significantly in both groups, but the decrease was significantly greater in the control group. The score on each item of the QOL evaluation was not significantly different between the two groups. After the second phase, blood pressure had decreased further in the observation group, but no significant change was observed in the control group, and there was no significant difference in blood pressure between two groups. The scores on each item of the QOL evaluation were not significantly different between the two groups, but average somatic symptoms, healthy and pleasant feelings, task perform-ance and life satisfaction were significantly ameliorated in both groups. Conclusions Music-regulated laser therapy can lower the blood pressure of mild and moderate PH patients effectively. Compared with antihyperten-sion drugs, music-regulated laser therapy provides a weaker effect in lowering blood pressure, but plays an e-quivalent role in improving the QOL of patients.

Objective Using surface electromyography (sEMG) techniques to investigate the patterns of frequency domain indexes of sEMC signals recorded from adolescent idiopathic scoliosis (AIS) patients' paraspinal muscles. Methods Twenty-five AIS patients were enrolled. All the patients undertook the Biering Sorensen test ( BST) ,the object-lifting test and a semi-bridge test, the ME3000P sEMG instrument was applied to record the electric activities of paraspinal muscles (convex/concave) of all subjects, and the time domain indexes such as averaged EMG(AEMG)and spectrum area(SPA) were analyzed, values in three tests were compared with one way ANOVA. Results The differences of AEMGs and SPAs on both convex and concave sides among the three tests showed no statistical significances (P = 0. 10,0.23, 0. 24 and 0. 45, respectively). Conclusions Any of the Biering Sorensen test( BST) , the object-lifting test or the semi-bridge test can be used to test the electric activities of paraspinal muscles (convex/concave) of AIS patients.

Objective To evaluate the clinical application of computer-aided electromagnetic imaging navigation in nasal endoscopic surgery.Methods Twenty-two cases of nasal endoscopic surgery with intraoperative imaging navigation were retrospectively reviewed,including 16 cases of sinusitis with or without polyp;5 cases of nasal inverting papilloma; 1 case of maxillary capillary hemangioma.All cases were operated with computer-aided electromagentic imaging navigation and nasal endoscope.Results The preoperative preparing time would take 4-10 minutes.In 22 cases,the localization accuracy between 3-D image landmarks of navigation system and actual anatomical landmarks was less than 1 mm.The optic nerve and other anatomical landmarks could be orientated accurately in intraoperative procedures.No complication occurred.Conclusions Nasal endoscope combined with computer-aided electromagnetic imaging navigation provides accurate anatomical localization of nasal cavity,sinuses and anterior skull base.It could improve the effectiveness and decrease surgical complications,especially in complicated cases.