Objectives To study the liver protective effect of L arginine for postoperative cirrhotic patients with primary liver cancer (PLC) receiving total parenteral nutrition (TPN). Methods Thirty preoperative cases were divided into two groups:Group A,TPN only( n =14);Group B,TPN+arginine( n =16). Liver function, serum lipid, immune function and serum nitric oxide were observed on preoperative phase, postoperative day 1 (POD 1) and day 7 (POD7) respectively. Liver biopsy was taken for histological study intraoperatively and on POD 7. Results Compared with group A on POD7,AST and TBIL level were near preoperative level, ALB level signiflcanly decreased, TG was lower than preoperative level, CD4 + and NO increased signiflcanly,and the degree of liver steatosis showed a signiflcant decrease in group B. Conclusions Supplement of appropriate amounts of L arginine increases hepatic NO level, reduces serverity of hepatic steatosis associated with TPN, promotes postoperative recovery of transaminase activity, ameliorates cell mediated immunity and anti infection capacity in postoperative PLC patients.

Objective To study the use on curative effect of Chinese medicine Qingyitang in severe acute pancreatitis.Methods 132 cases of severe acute pancreatitis were randomly divided into 2 groups,The control group(n=62) and the treatment group(n=70).Routine treatment were given in both,otherwise,the Chinese medicine Qingyitang was used in the treatment group.The operation rate,the infection rate,the time of abdominal distension removing and fasting and being hospitalized were observed and compared between two groups.Results Every index of the treatment group was finer than the control group.Conclusions The Chinese medicine Qingyitang showes their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disturbance following acute pancreatitis.As a result,the chances of bacterial translocation and enterogenic infection declines.These preparation might raise the treatment effects,as a well,shorten the course of treatment.

Objective To investigate the effects of urotensinⅡ(UⅡ)receptor antagonist(URA)on UⅡ in plasma and bronchoalveolar lavage fluid(BALF)in rats with acute lung injury(ALI).Methods Twenty eight Sprague Dawley(SD)rats were randomly divided into four groups:saline control group(group A),the other three groups(group B,group C,group D).Rats were injected with oleic acid and URA to produce ALI models.Three hours after injection,arterial blood was drawn for blood gas analysis in all rats.Immediately after this,blood of heart was collected in group A and group B.Then blood of heart were collected after 12 hours in group C and after 24 hours in group D.Rats were killed when blood of heart was drawn.Bronchoalveolar lavage(BAL)with saline was performed in the right lung.BALF was centrifugated and the upper fluid was put to the EP tube.Plasma was separated from the blood of heart.Both plasma and BALF were kept at -80 ℃ to be determined.Results Compared with group A,arterial pressure of oxygen was significantly decreased in the group B,group C,group D(P

Objective To study the effect of endoscopic treatment in acute severe pancreafitis. Methods EST(endoscopic sphincterepapiilotomy) and ENBD ( endoscopic naso-billary dralnage) or ENPD ( endoscopic naso-pancreatic drainage) were used to treat the acute severe pancreatitis(ASP). Results The recover time of blood dia-stase of the endoscopic therapy group and the contrast group was (3.2±1.5) days, (6.6±1.2) days, respectively (P<0.01) ; the incidence of needing to be operated was 0.7% (1/140), 6.5% (8/124), respectively (P<0.05) ;mortality rate was 0:7% (1/140) ,7. 3% (9/124) ,respectively(P <0. 01 ) ;the incidence of complication was 3.6% (5/140) ,41.9% (52/124), respecfivly(P<0.01). Conclusion EST + ENBD(or ENPD) in treating ASP has cer-tain effect and is the primary therapy to the ASP.

ObjectiveTo observe the effect of forced expansion of occlusion on radiation ankylosis of tempormandibular joint of patients with nasopharynx cancer after radiotherapy.Methods166 patients with ankylosis of tempormandibular joint were randomly divided into the treatment group (83 cases, treated with forced expansion of occlusion) and control group (83 cases, with other treatment).ResultsThe absoluteness value of degree of mouth opening in the treatment group was significantly higher than that in the control group( P<0.01), and the course of ankylosis of tempormandibular joint in the treatment group was obviously delayed compared with the control group ( P<0.01).ConclusionThe forced expansion of occlusion can delay the course of ankylosis of tempormandibular joint, expand the degree of mouth opening, recover or partially recover the occlusion and chaw function.

Adolescent ; Child ; Humans ; Insulin Resistance ; Metabolic Syndrome ; diagnosis ; prevention & control

ObjectiveTo investigate the effect of Xuebijing (Chinese herbal medicine preparation) on lung fibrosis in a rat model of oleic acid - induced lung injury.MethodsSixty SPF level of male Wistar rats were randomly ( random number) divided into three groups ( n =20 ):normal control group ( group A),oleic acid model group (group B),oleic acid and Xuebijing group (group C).Rats in group A were injected with normal saline (0.13 ml/kg) ; acute lung injury (ALI) model rats were injected with oleic acid (0. 13 ml/kg) intravenously in B and C groups.Twelve hours after administrations of saline or oleic acid into rats of three groups separately,samples of arterial blood from all rats were taken for blood gas analysis.By analysis of blood oxygen partial pressure,the acute lung injury model successfully made was confirmed.Then the rats in group C were treated with xuebijing (4 ml/kg) intravenously,once a day from the 1st to 7th day.The rats in groups A and B were treated with saline (4 ml/kg).Five rats in each group were randomly sacrificed on the 1st,7th,14th,28th day,separately.The bronchoalveolar lavage (BAL) of left lung of all rats was carried out after weight of lung weighed.The lefi lungs of rats were weighed in wet and dry in order to get the wet/dry ratio.Venous blood and BAL fluid (BALF) were obtained for detection of procollagen Ⅲ ( PC Ⅲ ) concentrations with enzyme - linked immunosorbent assay (ELISA).Right upper lobe of lung specimens was taken for histopathological examination after paraffin - embedded sections with hematoxylin-eosin (HE) staining and Masson staining.Multiple comparisons were analyzed by using oneway analysis of variance (SNK-q test).Statistical analyses were performed by using SPSS 13.0 software.ResultsOxygenation index of groups B and C ( PaO2/FiO2 ＜ 300) significantly decreased compared with the group A ( P ＜ 0.01 ).Levels of serum PC Ⅲ on the 14th day and 28th day in group C decreased significantly in comparison with group B ( P ＜ 0.05 ).Compared with group A,the levels of BALF PC Ⅲ and serum PC Ⅲ in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.01 ).Levels of PC Ⅲ in BALF on the 7th day,14th day and 28th day in C group decreased in comparison with group B ( P ＜0.01 ).Compared with group A,the levels of PCⅢ in BALF in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.05 ).ConclusionsThe result suggests that treatment with xuebijing may be useful in inhibiting pulmonary fibrosis during the course of ALI in rots.

Objective To resolve the problem of the accuracy and standardization of STR-PCR typing in forensic practice,construct DXS6804 allelic ladder by molecular clonning and apply them in a population study on the Pumi population in Yunnan,China.Methods PCR was used to produce several different allelic fragments of the locus.After cloning the PCR products,the recombinant plasmids were sequenced.Then we denominated them and used them as template for re-amplification to generate the locus standard ladder.Results The sequencing results confirmed that the size and the construction of the inserts were correct.The genetic polymorphisms of this locus in Yunnan Pumi population of China were studied.Two off-ladder alleles of DXS6804 locus were found.Conclusion This method is of high value for forensic DNA typing to construct standard ladders.DXS6804 is robust for genetic research and forensic application.

Objective To obtain an accurate prevalence of dementia and Alzheimer's disease (AD) in large sample of ≥75 year old elderly living in Guangzhou city. Methods From August 1994 to December 1995, the study was conducted in Liwan District of Guangzhou, China. A two stage cluster sampling design was used to obtain a large sample. ‘Jiedao’ (resident unit in China) was the primary sampling units (PSU),‘Jumin Xiaozu’ (resident group) was the secondary sampling units (SSU). Systematic sampling and simple random sampling procedures were used for the first and second stages, respectively. The study was divided into two phases: a door to door survey of the entire sample aged ≥75 years (4 735 persons), followed by a clinical examination of suspected cases for positive and differential diagnosis. In the first phase, mini mental status examination (MMSE) was used as screening method for dementia. In the second phase, dementia and AD were clinically diagnosed according to the criteria of Diagnostic and Statistical Manual for Mental Disorders (DSM III R) , National Institute of Neurological and Communicative Disorders (NINCDS) and Stroke Alzheimer's Disease and Related Disorders Association (ADRDA). Results The sensitivity and specificity of MMSE were 0 7451 and 0 7496 respectively while the positive predictive value was 0 2251. The prevalence of dementia in individuals aged 75 years and over was 8 90%, in which male and female were found to be 2 91% and 13 11%, and that of AD was 7 49%, male and female found to be 2 01% and 11 36% respectively. The prevalence of vascular dementia (VD) was 1 16%. Of the subjects with dementia, 84 24% was diagnosed Alzheimer's disease, 13% VD, and 2 76% dementia with other causes. The proportion of AD increased obviously by every 5 years. Conclusions The prevalence of dementia in ≥ 75 year persons was lower in Guangzhou than that in Shanghai, but was higher than that in other regional studies within China. In ≥75 year male and/or poorly educated female were more easily suffered from AD. The proportion of AD in dementia was higher in this study than that in others. These figures indicated that as a screening tool for dementia MMSE need improving and should be combined with ADL and POD together.

Objective To investigate angiographic manifestations and interventional treatment of non-acquired renal arterio-venous fistula.Methods Images of 13 cases of renal angiography were studied.Results The diagnosis of renal arteriovenous fistula was achieved,the rich findings were discussed.Conclusion Renal angiography is belived to be the best diagnostic method of the disease.Superselective renal arterial thrombosis is safe and effective and become the first choice in treatment.