AIM: To observe the effect of curcumin on the retinal structure and the expressions of interleukin-23 (IL-23) and interleukin-17(IL-17) in the rat retina after retinal ischemia-reperfusion injury (RIRI).METHODS:A total of 60 male Sprague-Dawley(SD) rats were randomly divided into normal control group(NCG),model group(MG),low-dose curcumin group (LDCG) and high-dose curcumin group (HDCG) (n=15 per group).RIRI was generated by anterior chamber perfusion of normal saline to the right eye.Rats in LDCG and HDCG received an intraperitoneal injection of 20mg/kg/d and 100mg/kg/d curcumin respectively,at 30min before RIRI and once daily after RIRI.Retinal structure and inflammation were evaluated after hematoxylin and eosin-stained (HE) staining.Western-blot and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-23 and IL-17 expressions after RIRI.RESULTS: The retinal structure of NCG was normal.Retinal edema,empty spaces or loosely packed cells and inflammatory cell infiltration were observed in MG and LDCG groups,whereas the morphological changes in HDCG group were improved as compared to MG and LDCG groups.Western-blot assay and ELISA showed that IL-23 and IL-17 expressions increased significantly after RIRI (vs NCG,P<0.01).Moreover,curcumin reduced IL-23 and IL-17 expressions significantly (vs MG,P<0.01).CONCLUSION: Curcumin can inhibit leukocytes infiltration and improve the retinal pathologic changes.Furthermore,curcumin can reduce retinal IL-23 and IL-17 expressions significantly in a dose-dependent manner.

Cardiac Catheterization ; methods ; Heart Defects, Congenital ; therapy ; Humans

This study took herbal medicinePoria cocosas an example to establish a method to explore the potential function of herbal medicine. And then, the new function was identified through the systematic study. It was able to improve the application range of herbal medicine and the clinical therapeutic effects. The established database management system ofPu-Ji-Fanghad been used for the searching ofPoria cocosin the treatment of different diseases in order to receive the compound prescription containedPoria cocos in different diseases. Then, indications recorded in herbalism literatures through dynasties and thePharmacopoeia of People’s Republic of China(Chinese Pharmacopoeia) were compared. Through statistical analysis and logical judgment, as well as the combination of modern pharmacological research results and clinical therapeutic evaluations, the overall understanding on conventional and potential functions ofPoria cocos were received. The results showed that the functions ofPoria cocos summarized through dynasties contained alleviating thirst, eliminating phlegm, enhancing memory, astringent, relieving cough and vomiting, invigorating blood circulation, promoting appetite and securing fetus, as well as treatment of deficiency, vomiting, stroke, diabetes, cough, cold damage syndrome, convulsion and other syndrome, were not recorded in theChinese Pharmacopoeia. It was concluded that tonifying kidney, relieving cough and phlegm, relieving vomiting, alleviating thirst, expelling wind, relieving convulsion and securing fetus were the potential function ofPoria cocos, which should arouse the attention from the academic community.

ObjectiveTo discuss the application of medical elastic stockings in nursing of patients with deep venous thrombosis formation. Methods62 patients diagnosed as acute lower limbs deep ve-nous thrombosis from January, 2005 to January, 2008 were randomly divided into the experimental group and the control groups with 31 patients in each group, the control group took general nursing measures, the experimental group used the medical elastic stockings based on the general care measures, The inci-dence of post- thrombosis syndrome was observed in two groups and underwent χ2 test. ResultsAfter two years follow- up, 2 patients relapsed and 3 patients appeared post- thrombosis syndrome in the experimental group, 8 patients relapsed and 10 patients had post-thrombosis syndrome in the control group. The relapse rate and incidence rate of peat-thrombosis syndrome in the control group were higher than those of the experimental group. ConclusionsThe use of medical elastic stockings can prevent acute lower limbs deep venous throm-bosis, and can reduce the symptoms of post-thrombosis syndrome, which deserves to be spread widely.

Objective To investigate the serum procalcitonin (PCT),interleukin-6 (IL-6 ),hypersensitive C-reactive protein (hs-CRP)and white blood cells (WBC)joint detection in the value of early clinical diagnosis of neonatal sepsis.Methods Choose 84 cases with sepsis and 140 cases of mormal newborn in children between 2013.6.1~2013.8.31.Used the electro-chemical luminescence method and scattering turbidimetric method to detect levels of serum PCT,IL-6 and hs-CRP respec-tively,at the same time test whole blood white cells with XE-2100 instrument.Results There were significant difference be-tween serum levels of PCT,hs-CRP and IL-6 of the patients with sepsis and normal newborns (t=6.37~10.58,P<0.01) and no statistically significant difference for WBC (t=3.15,P>3.15).But joint detection of PCT,IL-6 and hs-CRP for neo-natal sepsis,the sensitivity was 98.81%,specificity was 90.24%,higher than any of them a detection sensitivity and special-ity degree.PCT in detection of neonatal sepsis,when 0.5 ng/ml≤PCT≤2 ng/ml,it was priority to with gram-positive bac-terium infection,and when PCT>2 ng/ml,was priority to with gram-negative bacteria infection,compared G+ bacteria infec-tion and G- bacteria infection,the difference was statistically significant (χ2=4.646.37~35.05,P<0.05).Conclusion PCT,hs-CRP and IL-6 detection has high sensitivity,strong specificity,they can provide evidence for early diagnosis and treatment of neonatal sepsis.

Objective To investigate the influence on the proliferation of the precursor cells from the inner ear of SD rats by different ages and growth factors in vitro .Methods The organs of utricle or saccule or cochlear Corti were isolated from different days’ SD rats born on different days(P1 ,P7 ,P14 ,P21 ,P30 ,P60) .From 12 SD rats of different age ,with 24 ears ,precursor cells were dissociated and cultivated under non -adherent conditions as single cell .We then counted the number of cell spheres under inverted phase contrast microscope .The results were statisti-cally anayzed and the quantity change curve of the same organ in different age rats were described until the single cells were cultured for 7 days .The culture medium was supplemented with defferent growth factor to observe the effects of the grow th factor on the number of cell spheres .Immunofluorescence assays were conducted for phenotype characterization .Results ① Some newly isolated single cells ,labeled by nestin ,were from each inner ear organ of rats(P1) .During 7 days in culture ,cells kept proliferation and formed suspended spheres ,which were expressed Br-dU and nestin positive .② The cell spheres could only formed from each cochlear Corti of rats (P1 ,P7) .But cell spheres that formed from each utricle or saccule were observed in a single saccule culture medium of all rats (P1~P60) .③The number of cell spheres ,which were formed by the isolated cells from inner ear organs of single utricle or saccule or cochlear Corti ,increased obviously in the presence of EGF or bFGF or IGF -1 ,but were not obviously in LIF .Statistically significant differences were seen between control group and every experimental group including EGF group ,bFGF group ,IGF-1 group(P<0 .01) ,but were not seen between control group and LIF group (P>0 .05) .Conclusion The highly proliferative cells were present in the vestibular organ (utricle or saccule) and cochle-ar Corti of SD rats .They could express the characterization of neural stem cell .As increase of the age ,the overall trend of proliferative capability is declining .Each of EGF and bFGF and IGF -1 possess the promoting effects for proliferation on the precursor cells alone ,which is not in present of LIF .

Objective:To evaluate the characteristics of fusion using biomimetic n-HA/PA66 interbody cage with rhBMP-2 and discuss the feasibility of it.Methods:Thirty adult female goats underwent C_(3-4) discectomy and interverbral fusion performed with nHA/PA66 interbody cage without rhBMP-2(n=10).nHA/PA66 interbody cage with rhBMP-2(n=10),autogenous iliac crest bone graft(n=10).X ray was carried out at 4 and12 weeks after surgery to evaluate fusion quality.The goats were killed respectively on the 12th week.Nondestructive biomechanical testing for stiffness,un-decalcified histology were performed.Result:The goats in the n-HA/PA66 with rhBMP-2 group and the autogrft group all showed complete fusion at 12th week.there was no statisti- cally significant differience between the n-HA/PA66 with rhBMP-2 group and the autogrft group upon biomechanics,radiography and histomorphology.Conclusion:The interverbral fusion cage with n-HA/PA66 has good hiocompatibifity and osteoconductive ability and it may be good osteoionductive with rhBMP-2,so it was one ideal interbody fusion cage.

0.05).Conclusion The intervertebral fusion cage of n-HA/PA66 has good biocompatibility and osteo-conductibility.It may be the ideal interbody fusion cage.

Objective Explore the effect lactobacillus on allergic rhinitis (AR) rat serum Il-4 ,Il-5 ,IFN-γ concentration and the influence of immune markers .Methods SD rat 60 were randomly divided into 4 groups ,each group of 10 only .Lactic acid bacteria group every day with lactobacillus living bacterium preparations irrigation stomach 3 weeks ,establish rats LuanBai protein sensitiza-tion model ,positive drug control group irrigation stomach loratadine ;Another 20 rats used for passive cutaneous anaphylaxis (PCA) ,inspection in the rat model preparation and treatment .Determination test group rats serum IL-4 ,IL-5 ,IFN-γ,the content of Eos in nasal secretion was detection .Results Model group IL-4 ,IL-5 content was significantly higher than normal control group (P<0 .05) ,IFN-γlevels were low ,lower than the normal control group(P<0 .05) .Lactic acid bacteria group IL-4 ,IL-5 content were significantly lower than the model group(P<0 .05) ,IFN-γcontent increased ,higher than the model group(P<0 .05) .The in-dex of lactobacillus group with positive drug compared difference was statistically significant .Conclusion Lactic acid bacteria can cut AR rats serum IL-4 ,IL-5 level ,increase IFN-γlevel ,adjust Th1/Th2 lymphocyte subsets balance ,reduce nasal mucosa allergic inflammation .

Objective To investigate the treatment and clinical result of the elderly patients with incarcerated groin hernias caused acute abdomen.Methods We collected 35 cases of the elderly incarcerated groin hernias which can't be reducted and be treated by emergency operation from January 2011 to January 2016 in the PKUCare Luzhong Hospital.All patients' were analyzed by general condition,surgical procedures,surgical time,complications,length of hospital stay and follow-up.Results All 35 cases of the elderly incarcerated groin hernias were accepted surgical treatment,onset time were from 2 hour to 48 hour,18 cases of all were treated by high ligation of hernia sac;10 cases were treated by traditional hernioplasty;7 cases were tension-free hernioplasty.The surgical time was from 60 minutes to 140 minutes,The average time was 85 minutes.There were 5 patients had postoperative complications.Among them,1 case of patients with incarcerated groin hernias was reducted and happened the rupture of sigmoid colon and septic shock,resulting in multiple organ failure,3 days after the rescue invalid death;1 cases of postoperative intestinal obstruction,2 cases of incision infection,1 cases of hematoma of scrotum,All patients recovered well after treatment.The length of hospital stay was 3 ～ 13 d,average 7 d;patients were followed up for more than 6 months,there were 2 patients with inguinal hernia recurrence,and the other patients did not have symptoms.Conclusion Elderly patients with inguinal hernias caused acute abdomen should be preferred treatment of acute abdomen and be taken timely surgical treatment,to minimize bowel necrosis,abdominal infection,recurrence of hernia and other complications and promote the rehabilitation of patients.