Aim To investigate the bio-affinities of ligustilide and butylidenephthalide to rat aortic smooth muscle cells and the inhibitory effects of them on bFGF-stimulated proliferation of rat vascular smooth muscle cell (VSMC). Methods VSMCs were cultured from rat aorta pectoralis and identified by an immunohistochemical method. The bio-affinities between solute (ligustilide or butylidenephthalide) and cell membrane were measured by rat aortic cell membrane chromatography (CMC). The inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated VSMC proliferation were evaluated by MTT colorimetric method. Results Both ligustilide and butylidenephthalide had selective affinities to rat aortic smooth muscle cell as the same as verapamil, one of the calcium ion antagonists. They could potently separately (P ＜ 0.05 ), but had no effects on the normal VSMC growth. Conclusion Both ligustilide and butylidenephthalide can inhibit the abnormal proliferation of VSMC induced by bFGF.

BACKGROUND:Biomaterial combined with stem cels is revealing a bright future in bone tissue regeneration in orthopedic surgery. Induced pluripotent stem cels have better cel sources and characteristic, which have become a hotspot of stem cel field. OBJECTIVE:To review the research history, preparation method, cel characteristics of induced pluripotent stem cels and the research developments in orthopeadic surgery so far. METHODS:A computer-based search of CNKI and PubMed was performed for articles about induced pluripotent stem cels and their applications in the field of orthopeadic surgery published from 1999 to 2014. Typical and creative research achievements were enroled in result analysis. RESULTS AND CONCLUSION: Studies have shown that induced pluripotent stem cels have a potential application prospect in bone tissue regeneration, and they also show a satisfying biocompatibility with various scaffold materials, in which, the induced pluripotent stem cels can maintain a good osteogenetic potential. Detection methods and smal-molecule compounds have been discovered gradualy for cartilage regeneration. But how to effectively induce the chondrogenic differentiation of induced pluripotent stem cels and to realize the clinical applications need further research.

Objective To explore the effect RNAi-mediated inhibition of X-linked inhibit apoptosis protein (XIAP) gene on apoptosis and susceptibility to chemical therapy drugs in osteosarcoma cells and its underlying mechanism.Methods shRNA plasmid of XIAP gene were constructed. MG63 cells were conventionally cultured and divided into untreated group (group A,non-transfection),blank plasmid group (group B,psiRNA-Con transfected),the experimental group (group C,psiRNA-XIAP transfected),and 2 chemical drug interfered groups,non-transfection+0.3 ?mol Dox group (A+Dox group) and psiRNA-XIAP transfection +0.3 ?mol Dox group (C+Dox group). The XIAP expression in MG63 cells was detected by Western blotting. The proliferation of above-mentioned MG63 cells was examined by MTT assay. Flow cytometry (FCM) was employed to detect the cell apoptosis,cell cycle and Caspase-9 expression. Results Group C had a significantly lower protein level of XIAP and MTT value than group A and B (P

Objective To establish a rate model of stable orthotopic liver transplantation using three-cuff technique.Methods 28 dead rats were dissected and the causes for death and the complication analyzed after liver transplantation using three-cuff method.Results The main postoperative complications included:bleeding,stenosis of liver superior vena cava,portal venous thrombus,respiration duct obstruction,gastrectasia,pneumothorax,adhesion of liver to diaphragm.Conclusion Most of 8 kinds of complications were related with operation.

Animals ; Dose-Response Relationship, Drug ; Humans ; Inhalation Exposure ; Mice ; Nanoparticles ; toxicity ; Rats ; Siphonaptera

Objective To evaluate therapeutic effects of portal azygous devascularization on patients with portal hypertension. Methods In this study, data of 212 patients with portal hypertension who underwent devascularization were analyzed retrospectively. Among them 52% patients had a history of variceal bleeding. Preoperative Child A accounted for 15% patients, B for 57% and C for 27%. Emergent operation and selective operation were performed in 11% and 89% cases, respectively. Hassab procedure was performed in 176 cases (83%), whereas modified Sugiura procedure in 36 cases (17%). Results Portal vein pressure decreased from (40?6) cm?H 2O to (36?7) cm?H 2O. The re-bleeding rate of 3, 5,10 years were 2%, 6%, 11%, and the survival rates of 3, 5, 10 years were 97%, 92% and 89%, respectively. Conclusions Devascularization is an effective procedure in controlling bleeding with low incidence of liver failure, operation mortality and encephalopathy. Emergent operation should be avoided as it causes high morbidity and mortality. The decreased portal pressure following devascularization may result in a reduced blood supply to the liver. Persistent exasperation of the liver function caused by chronic hepatitis and the development of hepatocellular carcinoma are the two major factors endangering long-term survival.

Objective To observe the effects of Huangqi injection associated with a combined treatment on changes of hemodynamics indexes and brain natriuretic peptide (BNP) after cardiopulmonary resuscitation (CPR) in rabbit models with cardiac arrest (CA) to elucidate the mechanism of such cardiac protective effects. Methods Twenty-four New Zealand rabbits were randomly divided into four groups(each,n=6). The CPR model in rabbits was reproduced by using extra-corporal electric shock method(put through 50 V alternating current to quiver on chest). The animals in operation control group were given anesthesia,all kinds of catheter inserted into the body and tracheotomy,but no induction of ventricular fibrillation. In the epinephrine group,epinephrine (30 μg/kg)was injected into the right jugular vein by a catheter during CPR. In the sub-hypothermia group, epinephrine(30μg/kg)was injected into the right jugular vein via a catheter and 0.9%sodium chloride under 4℃at a rate of 1.0 mL · kg-1 · min-1 was pumped into the ear marginal vein simultaneously with the chest external compressions during CPR and the cooling of body surface sustaining at the target temperature (32-34℃) for 4 hours until the end of the experiment. In the combined treatment group,Huangqi injection(4 g/kg)was injected into the right jugular vein on the basis of treatment of sub-hypothermia group. Hemodynamics indexes such as left ventricular end-diastolic pressure(LVEDP),the maximal rate of left ventricular pressure increase/decline(±dp/dt max),and the mean arterial pressure(MAP)were dynamically monitored at 15 minutes before inducing ventricular fibrillation and at the early stage of post resuscitation for 30,60,120,180,240 minutes,and in the mean time the concentration of BNP was dynamically monitored at 15 minutes before inducing ventricular fibrillation and at the early stage of post resuscitation for 30,180,240 minutes. Results Compared with those of the operation control group,the levels of LVEDP and BNP of epinephrine,sub-hypothermia and combined treatment groups were gradually increased obviously,while ±dp/dt max and MAP were decreased significantly after successful CPR. The levels of LVEDP and BNP at any time point in the combined treatment group were markedly less than those of the epinephrine and sub-hypothermia groups,and along with the time extension,the level of LVEDP had a tendency of elevation,and the level of BNP had a tendency of gradual decrease,reaching peak and valley values respectively at 240 minutes after CPR〔LVEDP (mmHg,1 mmHg=0.133 kPa):6.56±0.21 vs. 12.57±0.33,9.54±0.24,BNP(ng/L):199±19 vs. 286±14, 251±29,all P<0.01〕,at each time point,the ±dp/dt max and MAP levels were significantly higher than those in epinephrine and sub-hypothermia groups,and they were gradually decreased with the time prolongation,reaching valley values at 240 minutes after CPR〔+dp/dt max(mmHg/ms):4 229±353 vs. 2 055±311,3 224±158,-dp/dt max(mmHg/ms):3 587±168 vs. 1 315±189,2 357±245,MAP(mmHg):82.02±1.81 vs. 44.15±1.17, 56.79±1.60,all P<0.01〕. Conclusion Huangqi injection associated with a combined treatment has obvious protective effects on heart after CPR in rabbits,and the mechanism may be related to improvement of hemodynamics indexes and inhibition of excessive expression of BNP.

In this article 108 human fetuses from 12 to 30 weeks of gestation, 3 neonates and a 1.5 years child were used for study of the histogenesis of the appendix.In 12 weeks old fetus, the appendix showed small lumen and thin wall. The mucosa presented intestinal villi and crypts. The endocrine and goblet cells could be found. The circular muscle and serous membrane had differentiated. In the specimens of 13 to 15 weeks, the lamina propria contained diffused lymphatic tissue and lymphatic follicles. On such sites, the intestinal villi were absent. The surface epithelium became attenuate and composed of cuboid or lower cuboid cells. The outer longitudinal muscle layer was evident. The scattered muscularis mucosa was found. At 16 weeks, the endocrine cells appeared and showed various forms. The open-type or closed-type endocrine cells could be distinguished easily. Under EM, based on the morphology of the basal granules, the endocrine cells might be divided into two types. The granules were cell enclosed in a limiting membrane. One type of the granules was round and various in size, and another type was irregular. In the surface epithelium covered on the lymphatic tissue, the microfold cells could be found. They showed irregular microvilli or mierofolds and numerous apical micropinocytotic vesicles. At 18.5 weeks, the villi tended to merge each other. At 20 weeks, the muscularis mucosa was formed and the 4 layers of appendix resembled those of the normal adult. We found endocrine cells aggregated into Segi's cap on the apex of the villi in 7 fetuses of 16-21 weeks. The T or B lymphocytes could be identified as early as 13.5 weeks by immunologic method. We found the percentage of the B lymphocytes decreased with the gestation age, and this fact suggested that the appendix might be a B lymphocyte pool during the fetal period.

Objective:To study the efficacy and safety of different types of quinolone antibiotics in the treatment of pelvic inflamma -tory diseases .Methods:Totally 70 patients with pelvic inflammatory diseases were selected and divided into the observation group and the control group according to the time of admission .The 35 patients in the observation group were treated with levofloxacin , and the con-trol group was treated with ciprofloxacin .After 20-day treatment, the clinical efficacy was observed and recorded in the two groups , and the adverse events were statistically analyzed .Results:The total effective rate of the observation group was 97.14%, which was signifi-cantly higher than that of the control group (82.86%, P<0.05).The recovery time of clinical symptoms and signs of the observation group was significantly shorter than that of the control group , and the difference was statistically significant (P<0.05).The difference in the incidence of adverse drug reactions was not statistically significant between the two groups (P>0.05).Conclusion:Levofloxacin in the treatment of patients with pelvic inflammatory diseates is with high clinical efficacy , which can rapidly improve the clinical symptoms and signs with high security .

Objective To explore the effect of individual nursing care on persistent headache in patients with intracranial aneurysm who accepted interventional embolization. Methods 100 patients with intracranial aneurysms were divided into observation group (n=50) and control group (n=50). The routine headache nursing care was taken in the control group and the individual nursing care was applied in the observation group. They were assessed with Visual Analogue Scale (VAS). Results The score of VAS reduced more in the observation group than in the control group (P<0.05), and the headache endurance and the satisfaction for nursing care were significantly better in the observation group than in the control group (P<0.05). Conclusion The individual nursing may relieve the pain, and improve the endurance of headache and the satisfaction for nursing care.