*Alkaloids ; *Antineoplastic Agents, Phytogenic ; Chemistry ; *Harringtonines

Objective To analyze the influence of anticoagulants on platelet count .Methods A total of 5 patients with decreased platelet level ,detected by using haemocyte analyzer and samples anticoagulated by ethylene diamine tetraacetic acid dipotassium (EDTA‐K2 ) ,were enrolled ,and EDTA‐K2 ,sodium citrate ,heparin lithium ,sodium fluoride anticoagulated venous blood samples and fingers peripheral blood samples were collected and detected for platelet by using haemocyte analyzer and microscopic detection . Wright‐Giemsa′s stained cell smears were prepared and observed by using microscope .Results Platelet levels of anticoagulated samples ,detected by haemocyte analyzer and microscopic detection were obviously decreased ,and with large platelet aggregation ob‐served under microscope .Platelet levels of finger peripheral blood samples detected by haemocyte analyzer and microscopic detection were normal ,and without platelet aggregation observed under microscope .Conclusion Various anticoagulants could cause pseudo‐thrombocytopenia ,and fingers peripheral blood samples ,without anticoagulants ,should be used for platelet count to obtain accurate results .

OBJECTIVE:To extract phenolic acid from Salvia militiorrhiza by multi-stage countercurrent extraction technology. METHODS: The multi-stage countercurrent extraction technology and traditional single pot decocting extraction technology were applied to extract phenolic acid from S. militiorrhiza. The content of salianic-acid B was determined and energy consumption of these extraction technologies were calculated and compared. RESULTS: As compared with traditional single pot decocting extraction technology, multi-stage countercurrent extraction technology effectively decreased the degradation of active component, energy consumption and production cycle. CONCLUSION: Multi-stage countercurrent extraction technology is suitable for the production of S. militiorrhiza.

Objective To explore the relationship between preoperative right ventricular ejection fraction(RVEF) and postoperative complications in the elderly patients with lung resection. Methods RVEF was assessed in 118 consecutive aged patients before pulmomary resection by echocardiography, 64 of whom received RVEF measurement again 3 weeks after operation. Results Preoperative RVEF in patients with postoperative complications was obviously lower than that in patients without ones. The frequency of postoperative complications in patients with different preoperative RVEF(

0.05).CONCLUSION:The domestic tropisetron hydrochloride is equivalent to the imported one in terms of the efficacy and safety in the treatment of cisplatin-induced nausea and vomiting.

Objective To examine and assess the clinical effect of Er ∶ YAG 2940 nm fractional laser resurfacing for skin wrinkles.Methods A total of 16 patients with Fitzpatrick skin type Ⅳ and facial wrinkles,were treated with Er ∶ YAG 2940 nm fractional laser resurfacing and follow-up were given every week after treatment,four weeks in all.Response to treatment was evaluated by observers and patients themselves.Photos were analyzed by the photoshop 12.0 software.Results During the four-week follow-up,the skin of patients was more and more better than pretreatment,and a large part of wrinkles was disappeared.The assessment of observers for wrinkle amelioration rose from (6.50--2.33) ％ to (34.80±5.24) ％ and the scores of content of patients was up to 4.00±0.27(generally satisfied),whereas no one satisfied at beginning.Analyzed by photoshop 12.0 software,the data of average and middle values were on the trend of increasing in all these four weeks while SD was basically on the trend of decreasing.Conclusions Fractional ablative photothemolysis using Er ∶ YAG 2940 nm laser is a promising option for skin wrinkles.

Objective To investigate the therapeutic effects of retinal angiomatosis in differentclinical stages.To discuss the indication of vitrectomy for retinal hemangioblastoma. Methods TheclinicaI data of 22 cases(33 eyes)were retrospectively analyzed.The retinal hemangiomas were dividedinto 5 stages according to their degrees of development from simple angioma without vessel dilation tofeeder vessel dilation and intra-retinal exudates.local retinal detachment,massive retinal detachment andcomplication occurrence in proper order.The methods of treatment were laser photocoagulation,trans-scleral cryotherapy and vitrectomy. 13 eyes were treated with laser photocoagulation, 5 eyes withcryotherapy combined with laser and 11 eyes with vitrectomy.Tumor resection and silicone oil tamponadewas performed in 3 eyes during vitrectomy.The patients were followed up for 46 months on average.Visual acuity(VA),the condition of the hemangioma and retina was compared pre～and post-operationrespectively. Results In a11 13 eyes treated with laser phot.coagulation the hemangiomas regressed andthe retina remained attached.VA improved in 2 eyes,and remained unchanged in 11 eyes.Cryotherapycombined with laser photocoagulation was performed on 5 eyes. In this group,4 eyes'hemangiomasregressed and no new hemangiomas occurred,proliferative vitreous retinopathy and vitreous hemorrhagewas observed in 1 eye which vitrectomy was performed later.VA improved in 2 eyes,unchanged in 2 eyesand decreased in 1 eye.In the 11 eyes treated with vitreoretinal surgery,new hemangiomas was found in 1eye,exudative retinal detachment was caused by hemangiomas in 2 eyes,proliferative vitreous retinopathywas observed in 2 eyes.and the retina remained attached in 8 eyes.VA improved in 3 eyes,unimproved in3 eyes,and decreased in 5 eyes.In the 3 eyes with surgical resection of retinal hemangioma during vitrectomy,2 eyes'retina remained attached,1 eye had exudative retinal detachment and no new hemangiomas occurred.VA improved in 2 eyes and decreased in 1 eye. Conclusions Laser photocoagulation or combined with cryotherapy is effective in treating the hemangiomas in early stage.Vitreetomy is advisable for late stage of retinal angiomatosis,especially with vitreous hemorrhage,epiretinal membrane,proliferation and large scale of retinal detachment.Surgical resection of isolated large retinal hemangioblastoma may be useful for selected patients.

Objective To investigate the efficiency of intracerebroventricular injection of histamine receptor antagonists on mechanical pain threshold of neuropathic pain in rats. Methods The neuropathic pain rat model was established by partially ligation of the sciatic nerve in 30 rats. Two weeks later, histamine receptor 1 antagonist pyrilamine 1.5 μg or 15 μg was injected intracerebroventricularly in group P1 or P2, histamine receptor 2 antagonist ranitidine 10 μg or 100 μg in group R1 or R2, and normal saline in group C as the control. The pain threshold was measured. Results Compaered with group C,the pain threshold was signifigantly increased in group R1 and R2, especially in group R2 (P<0.05). Conclusion Histamine H2 receptor antagonist can increase pain threshold in rats with neuropathic pain.

Objective To investigate the clinical value of shortening double-J stent indwelling time in the treatment of ureteral stone-street complications after flexible ureteroscope lithotripsy . Methods Clinical data of 38 cases of ureteral stone-street complications following flexible ureteroscope lithotripsy from January 2012 to December 2013 in our hospital were retrospectively analyzed.The patients were divided into Group A (extubating the double-J stent two weeks after the surgery ) and Group B (retaining the double-J stent) with 19 cases in each group .The stone clearance results were compared . Results All the urinary calculus in Group A had been removed successfully , including 2 cases of renal colic , 3 cases of irritative symptoms of bladder and 3 cases of gross hematuria.Hospital cost was (766.5 ±153.7) yuan.Urinary calculus were successfully removed in 13 cases in Group B, including 19 cases of gross hematuria and 17 cases of irritative symptoms of bladder.No renal colic occurred.The hospital cost was (1251.2 ± 155.6) yuan.Compared with Group B , Group A has higher success rate of stone clearance , lower hospital cost and lower rate of bladder irritative symptoms and gross hematuria (P<0.05).There were no significant differences between the two groups in the occurrence rate of renal colic (P>0.05). Conclusions Removing the double-J stent 2 weeks after flexible ureteroscope lithotripsy resulted in higher stone clearance rate and less complications compared with retaining the double -J stent.It can reduce the occurrence of irritative symptoms bladder and gross hematuria .

OBJECTIVE:To explore the observation and nursing points of hemorrhage in intravenous thrombolysis after using alteplase. METHODS:128 patients underwent alteplase intravenous thrombolysis were selected as the research object. The occur-rence of hemorrhage in each part were observed within 48 hours after medication and nursed timely. RESULTS:128 patients re-ceived intravenous thrombolysis. 48 h later,26 patients suffered from hemorrhage complications (20.3%). Among them,there were 6 cases of nose bleeding (4.7%),8 cases of subcutaneous congestion (6.3%),6 cases of gingival bleeding (4.7%) and 6 cases of gastrointestinal bleeding(4.7%). There was no significant difference in the incidence of complications among those groups (P>0.05). The time of nose bleeding was (3.2 ± 1.3) h,the time of subcutaneous congestion (3.5 ± 1.2)h,the time of gingival bleeding(1.5±0.7)h,and the time of gastrointestinal bleeding(4.1±1.8)h. The time of gingival bleeding was earlier than that of other groups,with statistically significant difference(P<0.05). There was no statistically significant difference among other groups (P>0.05). CONCLUSIONS:In alteplase intravenous thrombolysis,nurses should closely monitor hemorrhage and complications, especially the complication of gingival bleeding. Find out the complications timely and nurse it as early as possible.