Objective To explore the frequencies and phenotype of myeloid and plasmacytoid dendritic cells (mDC and pDC) in chronic HCV infection and to investigate the relationships between DC frequencies and HCV viral load and serum ALT level. Methods PBMC were isolated from chronic HCV infected patients and healthy control. Multi-color flow cytometry was used to analyze the frequencies and surface marker expression on mDC and pDC. The relationship between DC frequencies and viral load and ALT level was also calculated. Results In comparison with healthy control, frequencies of mDC and pDC in chronic HCV infection were significantly decreased (0. 37 ± 0. 19 and 0. 19 ± 0. 12 vs 0. 51 ± 0. 18 and 0. 29 ± 0.13, P＜0.05). The frequency of mDC was negatively correlated with HCV viral load (r= -0.5878, P ＜ 0. 0001 ) and serum ALT level ( r = - 0. 4628 , P = 0. 003 ). Both costimulatory markers ( HLA-DR, CD83, CD86, and CD40) and coinhibitory marker (PD-L1) expression on mDC and pDC in HCV infection were increased (P＜0.01 for costimulatory marker, P＜0.05 or F＜0.01 for coinhibitory marker). Conclusion The frequencies of mDC and pDC in chronic HCV infection were decreased, while the expression of costimulatory markers and coinhibitory marker were increased or not decreased in HCV infection. The decreased frequency of mDC was probably related to persistance of HCV infection.

OBJECTIVE:To explore the observation and nursing points of hemorrhage in intravenous thrombolysis after using alteplase. METHODS:128 patients underwent alteplase intravenous thrombolysis were selected as the research object. The occur-rence of hemorrhage in each part were observed within 48 hours after medication and nursed timely. RESULTS:128 patients re-ceived intravenous thrombolysis. 48 h later,26 patients suffered from hemorrhage complications (20.3%). Among them,there were 6 cases of nose bleeding (4.7%),8 cases of subcutaneous congestion (6.3%),6 cases of gingival bleeding (4.7%) and 6 cases of gastrointestinal bleeding(4.7%). There was no significant difference in the incidence of complications among those groups (P>0.05). The time of nose bleeding was (3.2 ± 1.3) h,the time of subcutaneous congestion (3.5 ± 1.2)h,the time of gingival bleeding(1.5±0.7)h,and the time of gastrointestinal bleeding(4.1±1.8)h. The time of gingival bleeding was earlier than that of other groups,with statistically significant difference(P<0.05). There was no statistically significant difference among other groups (P>0.05). CONCLUSIONS:In alteplase intravenous thrombolysis,nurses should closely monitor hemorrhage and complications, especially the complication of gingival bleeding. Find out the complications timely and nurse it as early as possible.

ObjectiveTo investigate the clinical features of intramedullary spinal cord tumors(IMSCT) in children.MethodsThe clinical data including sex,age,pathohistology,clinical manifestations and medical check-up collected from 16 children diagnosed as IMSCT in Shengjing Hospital Affiliated to China Medical University,were retrospectively reviewed and analyzed.ResultsThe study subjects inclu-ded 11 male and 5 female,with an average age of(8.55?4.28) years old,ranged from 15 months to 14 years old.The interval lasted 1 day to 9 years from onset of the syndrome to confirmed diagnosis of IMSCT.The first clinical manifestation was mostly pain.The main clinical mani-festations included limb dyskinesia in 14 cases,pain in 12 cases,urine and stool disturbance in 9 cases,gait irregularity in 8 cases,extremity atrophy in 3 cases.Medical check-up included abnormal tendon reflex in 14 cases,abnormal superficial reflex in 9 cases,sphincter dilatation in 6 cases,positive Babinski's signs in 5 cases,spinal deformity in 4 cases,region masses in 3 cases,paraesthesia in 2 cases,inequality of lower limb in 1 case,dermai sinus in 1 case,pigmentation in 1 case.The cases were evaluated by magnetic resonance imaging,which presented thickened spinal cord and intramedullary abnormal signals in all the cases.Pathological types: teratoma in 5 cases,dermoid cysts in 2 cases,epidermoid cyst in 2 cases.Misdiagnoses included urinary tract infections in 2 cases,rheumatoid arthritis in 1 case,and lumbar disc protrusion in 1 case.ConclusionsThe main pathohistology of pediatric IMSCT is congenital tumor.First clinical manifestation is pain.MRI can show a reliable evidence for the diagnosis.It is important to improve the outcomes of pediatric IMSCT by establishing the diagnosis and administering proper management as early as possible.

Epidermal growth factor (EGF) is a very important peptide growth factor,which is not only involving in a variety of ocular cell mitosis,proliferation and differentiation,but also participates in mediating the signal transduction between cell and cell,cell and matrix interaction and other biological functions.Corneal injury is a common and frequently occurring disease in ophthalmology,and it would lead to blindness without appropriate treatment.Researches showed that EGF can promote the repair and regeneration of wounded cornea.In recent years,these studies advance from animal experiment stage to clinical stage and also from the morphology evaluation stage to the genetics study stage.This paper provided an overview of EGF concept,its biological effectiveness,its relationship to corneal injury and its clinical application.

Based on the theory of tumor angiogenesis,the vascular system is necessary for the advance and metastasis of the tumor.To inhibit or even cure the tumor,the researchers all over the world have made a great many of studies on the mechanism of tumor angiogenesis and have acquired some achievements.Based on the findings,a lot of factors are involved in tumor angiogenesis,and they constitute the complex regulating network through interaction at the molecular and cellular level.

With the growth of disease incidence in allergic diseases of upper respiratory tract year by year, airborne pollen has been considered the most important cause of the diseases. There is an obvious positive correlation between the concentration of airborne pollen and the symptoms of the diseases. The data is collected which includes environment that patient lives in about the kinds of airborne pollen and their seasonal distribution and latest conditions of the airborne pollen at home and abroad. It is benefit for the further developments of the researches of airborne pollen and has a clinical significance for the prevention, diagnosis and treatments of allergic diseases in our country.
Allergens ; immunology ; Meteorological Concepts ; Particulate Matter ; immunology ; Pollen ; immunology ; Seasons

Animals ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Hypoxia ; Cytokines ; metabolism ; Epithelial-Mesenchymal Transition ; Head and Neck Neoplasms ; metabolism ; pathology ; Humans ; Hypoxia-Inducible Factor 1 ; metabolism ; Transcription Factors ; metabolism

Biomedical Research ; Humans ; MicroRNAs ; Neoplasm Invasiveness ; genetics ; Neoplasm Metastasis ; genetics

Objective To explore the clinical features,maternal and fetal outcome in preeclampsia complications.(Methods)Clinical data of 86 cases of preeclampsia complications in heart,liver,kidney and brain were analyzed retrospectively.All the patients were divided into two groups: early onset preeclampsia(EOPE) group,36 cases,onset gestational weeks

Objective To analyze 85 cases of small bowel stromal tumor with follow-up results,and compare the clinical factors on the prognosis of disease. Methods Getting started from clinical records,a specific database was designed and clinical data concerning patient demographics,clinical findings,lab results,radiology reports,endoscopy reports,perioperative parameters,pathology reports and follow-up notes were input into the database. The influences of clinical factors on prognosis were compared by inquiring about the database. Results It was revealed through follow up that the maximal tumor diameter(relative risk,1.7) and tumor surface ulceration(relative risk,6.0) were two independent prognostic factors closely related to the survival(P