Since the endotoxic fever is mediated essentially by endogenous pyrogen (EP), we have examined the possibility that the central cAMP is involved in the mechanism of biphasic fever induced by endotoxin, as in the case of biphasic fever induced by EP. The results showed that administration of endotoxin in rabbits elicited typical biphasic fever. The fluctuation of concentration of cAMP in cerebrospinal fluid (CSF) paralleled with the change of the rectal temperature, similar to that of the bipbasic fever caused by EP. Raised level of cAMP in plasma was observed only in the first phase. The cAMP concentration in CSF correlated positively with the febrile response. The correlation of cAMP levels in CSF and plasma was observed only in the first phase but not in all the febrilic periods. These findings indicated that the mechanism of endotoxic biphasic fever is associated with the Change of central cAMP. Content. The raised level of cAMP in CSF during fever is likely to represent the biosynthsis in brain regions. However, the possibility that the raised cAMP in plasma enters CSF in the first phase is not excluded.

The signals of body temperature from 4 different febrile patterns were sampled for power spectral analysis. The results showed that the spectrums had no difference between their distribution in frequency domain, only a low frequency peak has been seen. The values of the power spectrum varied directly to the intensity of the febrile responses. A high degree of relationship was found between the power spectral density and thermal response index (TRI).

AIM: To study the mechanism of the inhibitory effect of human polymorphonuclear leukocytes (PMN) on the production of tumor necrosis factor-? (TNF-?) in U937 cells. METHODS: PMA differentiated U937 cells were cocultured with PMN in the presence of lipopolysaccharide. Sodium azide was added in the culture. The supernatants of cell culture were collected and TNF-? was assayed with ELISA. RESULTS:The inhibitory effect of PMN on the production of TNF-? in U937 cells was observed. Sodium azide restored the TNF-? production in U937 cells/PMN coculture to the extent of 45% in concentration of about 10～15 ?mol/L. These concentrations of sodium azide did not affect TNF-? production by U937 cells. The restoration effect of sodium azide on PMN inhibition was not related to blocking of myeloperoxidase (MPO) activity. The MPO inhibitors 3-amino-1,2,4,-triazole and 4-aminobenzoyl hydrazide had few effect on PMN inhibition. CONCLUSION: These results indicated that PMN inhibition depended on the cell-cell interactions. Sodium azide may be a useful reagent for further studying this mechanism.

Objective To explore the clinical features,diagnosis and treatment of solid-pseudopapillary tumor(SPT)of pancreas.Methods The clinical data of 19 patients with SPT treated in 8 years were studied retrospectively,included 3 males and 16 females.Results The type of operation was decided by the size and location of tumor.Pancreatic leakage was a familiar complication,there were 9 cases.Patients were followed up from 3 to 70 months,and no recurrence or metastsis was found.Conclusion Pancreas SPT is a rare type neoplasm of pancreas.Pancreas SPT is affecting primarily young women,complete resection results in excellent prognosis.

Objective To investigate the variations of serum levels of visfatin in pregnant women with different types of glucose intolerance and the associations with insulin resistance.metabolic parameters and newborn birth weight.Methods Totally,120 pregnant women in late trimester were divided into 3 groups according to the results of 50 g glucose challenge test(GCT)and 75 g oral glucose tolerance test:GDM group(n=45),GCT(+)group(n=20),and NGT group(n=55).Fasting levels of visfatin,insulin,total cholestrol and triglyceride were measured and the newborn birth weight was recorded.Results The serum visfatin level was significantly elevated in GDM group compared with the NGT group[(374.9±23.1)ng/ml vs(308.9±16.8)ng/ml,P＜0.05],but no significant difference was shown when compared with the GET(+)group[(346.5±23.1)ng/ml].Furthermore,serum visfatin levels had no relationship with insulin resistance,metabolic parameter and newbom birth weight.Conclusions Visfatin is elevated in GDM women and closely related with GDM,but not with insulin resistance,metabolic parameters and newborn birth weight.

Objective To evaluate the prevalence of aspirin resistance in old-aged patients with cerebral infarction and to analyze the mechanisms and clinical predictors of aspirin resistance. Methods 300 old-aged patients with cerebra] infarction were selected and the platelet aggregation ratio was determined by nephelometry with CHRONO-LOG PA-meter. And some information of these patients, such as age, sex, smoking, taking non-steroid anti-inflammatory drug (NSAD)or proton pump inhibitor (PPI), obesity, coronary artery disease (CAD), peripheral vascular disease, hyperpiesia, diabetes and hyperlipemia were recorded. Results 112 cases (37.33%) were aspirin sensitiveness, 108 cases(36.0%) were aspirin semi-resistance and 80 cases (26.67%) were aspirin resistance. These cases were divided into two groups. One was aspirin sensitive (AS) group, which included 112 patients of aspirin sensitiveness. The other was aspirin resistant (AR) group, which consisted of 188 patients of aspirin semi-resistance and aspirin resistance. The prevalence of CAD, hypercho]esteremia and RBC count in AR group was higher than that in AS group The percentage of patients who took NSAD, PPI and smoke in AR group was also higher than that in AS group. Conclusion Aspirin resistance existed in old-aged patients with cerebral infarction to great extent, and which was correlated with CAD, hypercholesteremia, smoking, RBC count, taking NSAD and PPI.

Objective To acknowledge the incidence of sharp- appliance wound of trainee nurses and explore the relative countermeasures. Methods A retrospective investigation was carried out in 140 trainee nurses of grade 2003 about the incidence of sharp- appliance wounds, then the corresponding pre-ventive countermeasures were formulated and applied in the clinical practice of trainee nurses of grade 2004 and the incidence of sharp- appliance wound was investigated and compared with the results of those grade 2003.X2 test was adopted. Results The incidence rate of sharp- appliance wound in trainee nurses of grade 2004 was obviously lower than that of grade 2003. Conclusions Due to the weak safety con-sciousness, unfamiliar with operation and lack of clinical experience, the sharp- appliance wound will easily occur among trainee nurses. If the relative professional preventive knowledge is strengthened, the opera-tional procedures are observed strictly and the sense of responsibility of tutor teachers is facilitated, then the incidence of sharp- appliance wound of trainee nurses will drop.

Objective:To study the correlations between ethics morality, society, psychological cognition and health and disease. Methods:A total of 356 patients were selected to analyze the influence to health and disease from six perspectives that included family ethics, social morality, life style, interpersonal relationship, mental health, and personality traits. Result:Psychosomatic diseases were occurred in 126 cases, and 220 cases of neuro-sis diseases. The disease incidences in the subjects with high cognitive level were significantly lower than those with low cognitive level (P<0. 01). The cognitive levels of family ethics, social ethics, life style, interpersonal rela-tionship, and mental health were closely related to health and disease (P<0. 05). Conclusion:The cognitive lev-els of ethics morality, society, psychology were closely associated with disease. It should be vigorously advocated that the whole society follow the ethics morality standards, to prevent diseases.

A new method of calculating fractal dimension of short-term heart rate variability signals is presented. The method is based on wavelet transform and filter banks. The implementation of the method is: First of all we pick-up the fractal component from HRV signals using wavelet transform. Next, we estimate the power spectrum distribution of fractal component using auto-regressive model, and we estimate parameter 7 using the least square method. Finally according to formula D = 2- (gamma-1)/2 estimate fractal dimension of HRV signal. To validate the stability and reliability of the proposed method, using fractional brown movement simulate 24 fractal signals that fractal value is 1.6 to validate, the result shows that the method has stability and reliability.
Algorithms ; Electrocardiography ; Fourier Analysis ; Fractals ; Heart Rate ; physiology ; Humans ; Least-Squares Analysis ; Nonlinear Dynamics ; Signal Processing, Computer-Assisted

Objective To study the relevance of peripheral blood mononuclear cell (PBMC) IL- 18 level in patients with chronic HBV infection and the activity of chronic hepatitis B as well as the ex- tent of hepatic tissue inflammation. Methods Flow cytometry was applied to measuring IL-18 level in PBMCs isolated from 147 patients with HBV infection; meanwhile, 22 cases with hepatitis B patients or HBV carriers underwent needling biopsy of liver for liver histopathologic examination. Results IL- 18 level was rising step by step in moderate chronic hepatitis, severe chronic hepatitis, chronic serious hepatitis and acute fatal hepatitis. Elevation of IL-18 level was correlative to the activity of hepatic tis- sue inflammation. Conclusion The expression level of IL-18 is different in various HBV infection states, which is correlative to the activity of chronic hepatitis B as well as the extent of hepatic tissue inflammation. Therefore, IL-18 may be used as an important index of evaluating therapeutic effect and prognosis.