Since the endotoxic fever is mediated essentially by endogenous pyrogen (EP), we have examined the possibility that the central cAMP is involved in the mechanism of biphasic fever induced by endotoxin, as in the case of biphasic fever induced by EP. The results showed that administration of endotoxin in rabbits elicited typical biphasic fever. The fluctuation of concentration of cAMP in cerebrospinal fluid (CSF) paralleled with the change of the rectal temperature, similar to that of the bipbasic fever caused by EP. Raised level of cAMP in plasma was observed only in the first phase. The cAMP concentration in CSF correlated positively with the febrile response. The correlation of cAMP levels in CSF and plasma was observed only in the first phase but not in all the febrilic periods. These findings indicated that the mechanism of endotoxic biphasic fever is associated with the Change of central cAMP. Content. The raised level of cAMP in CSF during fever is likely to represent the biosynthsis in brain regions. However, the possibility that the raised cAMP in plasma enters CSF in the first phase is not excluded.

The signals of body temperature from 4 different febrile patterns were sampled for power spectral analysis. The results showed that the spectrums had no difference between their distribution in frequency domain, only a low frequency peak has been seen. The values of the power spectrum varied directly to the intensity of the febrile responses. A high degree of relationship was found between the power spectral density and thermal response index (TRI).

AIM: To study the mechanism of the inhibitory effect of human polymorphonuclear leukocytes (PMN) on the production of tumor necrosis factor-? (TNF-?) in U937 cells. METHODS: PMA differentiated U937 cells were cocultured with PMN in the presence of lipopolysaccharide. Sodium azide was added in the culture. The supernatants of cell culture were collected and TNF-? was assayed with ELISA. RESULTS:The inhibitory effect of PMN on the production of TNF-? in U937 cells was observed. Sodium azide restored the TNF-? production in U937 cells/PMN coculture to the extent of 45% in concentration of about 10～15 ?mol/L. These concentrations of sodium azide did not affect TNF-? production by U937 cells. The restoration effect of sodium azide on PMN inhibition was not related to blocking of myeloperoxidase (MPO) activity. The MPO inhibitors 3-amino-1,2,4,-triazole and 4-aminobenzoyl hydrazide had few effect on PMN inhibition. CONCLUSION: These results indicated that PMN inhibition depended on the cell-cell interactions. Sodium azide may be a useful reagent for further studying this mechanism.

Objective To investigate the ontogeny of the islet amyloid polypeptide(IAPP)and explore the relationship IAPP and other classical hormones in human fetal colon and rectum. Methods The localization of IAPP immunoreactive(IR) cells and 5 hydroxytryptamine(5 HT) IR cells in 31 human fetal colon and rectum from 9\|27 weeks of gestation was investigated with immunohistochemical SABC method. Results At 9 weeks of fetal age,a lot of 5 HT IR cells were found in the colon,but IAPP IR cells appeared at 18 weeks.In the rectum,both 5 HT and IAPP IR cells were present at 11 weeks,the number of 5 HT IR cells ascended gradually with fetus aged,reached a peak at 20 weeks and dropped gradually after 21 weeks,while in the whole fetus period,IAPP IR cells appeared fewer and scattered.The co localization of IAPP and 5 HT in some cells of colon and rectum was proved as compared with adjacent sections.Immunohistochemical double staining procedures on the same tissue section also showed that IAPP co existed with 5 HT in some cells.Conclusion\ During the human fetus,IAPP were synthesized and co existed with 5 HT in endocrine cells of the colon and rectum.The possible function on the co existence of IAPP and 5 HT in the colon and rectum of fetal was discussed. [

Objective:To analyze the genetic sequence characteristics of amomum viosum and establish a rapid identification meth-od for amomum viosum by fluorescent quantitative PCR based on DNA analysis. Methods:Amomum viosum and the other samples be-longing to the same genera were collected and identified by experts in the domain. DNA was isolated using commercial kits. The prim-ers and probe were designed according to the conserved region of ITS in amomum viosum. The reaction conditions were optimized to es-tablish the fluorescent quantitative PCR method for the rapid detection of amomum viosum. Results:The fluorescent quantitative PCR method for the rapid detection of amomum viosum was set up. The method could identify amomum viosum successfully, while those samples in the same genera were without amplification curves. Conclusion: Amomum viosum can be identified rapidly by fluorescent quantitative PCR besides the traditional identification by experts.

The psychological status of oromaxillo-facial hemangiomas children's parents were investigated by the LES and the SAS,etc.All of the parents were in negative psychological status.But there was no obvious body symptom.Indigestion,dyssomnia,and nightmare were main body symtom.

The antagonistic effects of ruthenium red (RuR) on the biological activi-ties of endotoxin (ET) which included the limulus amebocytes lysate test, the local Shw-artzman reaction, decreasing the white blood cell counts, and increase of zinc in periphe-ral blood were observed. The destroyed meshes of endotoxin have been seen under electronmicroscope. The amino-groups of endotoxin increased markedly, which it was treated withRuR. These results indicated that the polycationic residues of RuR may be the effectiveunits of antiendotoxin activity.

Objective To investigate the variations of serum levels of visfatin in pregnant women with different types of glucose intolerance and the associations with insulin resistance.metabolic parameters and newborn birth weight.Methods Totally,120 pregnant women in late trimester were divided into 3 groups according to the results of 50 g glucose challenge test(GCT)and 75 g oral glucose tolerance test:GDM group(n=45),GCT(+)group(n=20),and NGT group(n=55).Fasting levels of visfatin,insulin,total cholestrol and triglyceride were measured and the newborn birth weight was recorded.Results The serum visfatin level was significantly elevated in GDM group compared with the NGT group[(374.9±23.1)ng/ml vs(308.9±16.8)ng/ml,P＜0.05],but no significant difference was shown when compared with the GET(+)group[(346.5±23.1)ng/ml].Furthermore,serum visfatin levels had no relationship with insulin resistance,metabolic parameter and newbom birth weight.Conclusions Visfatin is elevated in GDM women and closely related with GDM,but not with insulin resistance,metabolic parameters and newborn birth weight.

OBJECTIVE:To discuss the adjustment of patients'medication psychology in clinical pharmaceutical care.METHODS:Various factors that influence patients'medication psychology and the effects resulted from patients'medication psychology were analyzed.RESULTS&CONCLUSIONS:Patients'psychological effects influence the physiological effects of medicine,thus greatly influencing drug action,therefore,pharmacists'knowledge on pharmacopsychology should be strength?ened and their communication ability with patients should be improved.Furthermore,pharmacists should adjust patients'medication psychology timely to satisfy their basic psychological needs for medication.

Intracranial stent implantation induces platelet-monocyte aggregate, regulates activities of monocyte, induces monocyte expression tissue factors, and promotes fibrin deposition in thrombus at vessel injury site, which promotes inflammatory reaction. P substance significantly increases following stent implantation; platelet ? particle-derived soluble CD 40L, which can promote platelet aggregation, also increases after stenting. Flow cytometry, platelet aggregation, soluble platelet activation-related molecule in serum are used to determine platelet activation and aggregation in blood of 30 patients before and after stent implantation from Department of Neurosurgery, First Hospital of China Medical University, so as to explore blood coagulation reaction characters after intracranial stent implantation