Thymus derived CD+4; CD+25 regulatory T cells (Treg) are thought to be specific T cells that play an important role in controlling autoimmunity, preventing transplant rejection, restraining anti-infectious immune response, suppressing allogeneic immune respons. More recently, these cells are reported to have the ability of suppressing antitumor immune response. Foxp3 are the most specific protein of Treg. In this review, we will discuss the expression of CD+4 CD+25 Treg in patients with hematological malignancies and its implication for immunotherapy.

Objective:To enhance the functional safety of medical electrical equipment and reduce the risk to patient when using the medical electrical equipment.Methods: Theory and rationale introduction of functional safety and provide application cases.Results: Introduce the definition and application scope of functional safety standards IEC 61508, the determination of safety integrity level of functional safety in the Medical Electrical equipment, and the design structure and development, meanwhile show some application examples.Conclusion: Provide suggestions for the manufactures for safety design that introduce functional safety standards IEC 61508 to the development process of Medical Electrical equipment will help enhance the safety level of Medical Electrical equipment and reduce the unacceptable risk’s probability to patient.

Objective: To observe the short-term and long-term efficacy of acupuncture and traction plus Ba Duan Jin (Eight-brocade Exercise) in treating discogenic low back pain (DLBP). Methods: Sixty patients were divided into an observation group and a control group using the random number table method, with 30 cases in each group. The control group was intervened by acupuncture and traction, while the observation group was given additional Ba Duan Jin (Eight-brocade Exercise) practice. The intervention lasted for a total of 1 month, with a follow-up study conducted 6 months later. The visual analog scale (VAS) and Oswestry disability index (ODI) scores in the two groups were compared before and after treatment, and the efficacy was compared at the end of the treatment between the two groups; the relapse rate was compared at the 6-month follow-up between the two groups. Results: The pain VAS and ODI scores decreased after treatment in both groups, and the intra-group differences were statistically significant (all P<0.05); the pain VAS and ODI scores in the observation group were lower than those in the control group after treatment with statistical significance (both P<0.05). At the 6-month follow-up, the relapse rate was 10.3％ in the observation group versus 48.0％ in the control group, and the between-group difference was statistically significant (P<0.05). Conclusion: The combination of acupuncture, traction and Ba Duan Jin (Eight-brocade Exercise) is an effective method for DLBP and patients who received this method are less likely to relapse.

Apurinic/apyrimidinic endonuclease/redox factor 1(APE/Ref 1) is a multifunctional protein that is responsible for repairing DNA damage,it also infleunce reduction oxidation (redox) reaction and modulate the DNA binding activity of transcription factors,it is important to cell survival.Recently, much data concerning APE1/Ref 1 and information on APE1/Ref 1 in a wide variety of functions and systems have rapid growth,including its gene,function,distribution and relations with some diseases,especially nervous system diseases and tumors.

Objective To investigate the characteristics of laparoscopic cholecystectomy(LC) in the elderly. Methods A retrospective analysis was made on clinical data of 118 elderly patients treated with LC in this hospital between January 2000 and January 2005. Results The LC was successfully completed in 116 patients,while conversions to open surgery were required in 2 patients due to low level of insertion of the cystic duct(1 patient) and common bile duct injury(1 patient).The operation time was(30~120 min)(mean,50 min).The postoperative recovery was uneventful in all the patients.The length of hospital stay after operation was 3~7 d(mean,4.5 d).The patient with common bile duct injury was followed for 2 years and 6 months.Transient onsets of abdominal pain and fever were noted and cured with antibiotics for 2~5 days.No biliary tract stenosis or bile duct calculus was seen.Follow-up observations in the remaining 117 patients for 6~12 months(mean,10.5 months) revealed no abdominal pain,fever,or jaundice. Conclusions Laparoscopic cholecystectomy is acceptable and safe for elderly patients.Proper peri-operative management and careful surgical performance are essential to the success of surgery.

BACKGROUND: Interverbral disc degeneration (IDD) is the major cause of low back pain, which is considered as the pathological basis of intervertebral disc herniation and other degenerative diseases that severely affect the patients' quality of life. At present, neither conservative treatment nor operative treatment can reverse or inhibit the pathological development of IDD.OBJECTIVE: To summarize the progress of stem cell therapy for IDD, and to further analyze its challenge and potential solutions.METHODS: Relevant studies in PubMed database published before September 2016 were retrieved. Studies about stem cell therapy in IDD in vitro and vivo as well clinical trials were enrolled to analyze its existing problems.RESULTS AND CONCLUSION: (1) Stem cell therapy provides a new strategy for IDD treatment. So far, a large number of studies in vitro and vivo as well as clinical trials have reported the remarkable effect of stem cell therapy. (2) However, problems such as how to deal with microenvironment of interverbral disc, choose proper stem cells and master indications will be a challenge in the future.

Objective Using apomorphine, a potent dopamine receptor agonist and rotating T-maze, the effect of apomorphine on the visual discrimination learning and reversal learning in rats was investigated. Methods All rats were trained in a visual discrimination task (food reward and light stimulus) in rotating T-maze. After reaching the acquisition criterion, rats were trained in a reversal task (food reward and without light stimulus) in the same maze. During the period of visual discrimination task, apomorphine was administrated either 30 minutes prior to learning or after learning immediately. Results The results showed that apomorphine, which was given either 30 minutes prior to visual discrimination learning or after learning, could impair the acquisition of discrimination learning( 259.20±26.29 and 264.00±16.97, compared to 168.00±16.97 and 163.20±20.08) and apomorphine, which was given only after visual discrimination learning, could impair the acquisition of reversal learning (451.20±39.44 compared to 360.00±29.39). Conclusion The results showed that apomorphine, which was given either 30 minutes prior to visual discrimination learning or after learning, could impair the acquisition of discrimination learning and apomorphine, which was given only after visual discrimination learning, could impair the acquisition of reversal learning.

Anemia, Aplastic ; drug therapy ; immunology ; surgery ; therapy ; Bone Marrow Transplantation ; Child ; Humans ; Immunosuppressive Agents ; therapeutic use ; Treatment Outcome

Objective To analysis the progress in prevention and control of iodine deficiency disorders (IDD) in Dalian and to provide a scientific basis for further prevention and control of the disease.Methods From 2006 to 2010,5 or 9 townships were randomly sampled from each county in Dalian,4 villages were sampled from each selected townships and 15 or 8 households were sampled from each selected villages for collecting salt samples and salt iodine level was determined by direct titration method.Sixty daytime urine samples from pregnant women,breast feeding women,infants and young children were collected,respectively,every year to detect urinary iodine level.From 2007 to 2009,2 to 11 counties were sampled and from each selected county one school was sampled to collect 100 daytime urine samples of children aged 8-10 and iodine content was determined.From 2006 to 2009,2 to 5 counties were sampled,from each selected county 2 to 5 schools were sampled and 100 children aged 8 to 10 in each selected school were sampled to detect intelligence quotient level and the thyroid volume.Results From 2006 to 2010,16 012 copies of households' edible salt samples were monitored; the average iodine content was ranged 29.68-31.51 mg/kg,the rate of qualified iodized salt in household ranged from 97.24％ to 98.42％.A total of 1398 copies of urine samples of pregnant women,486 breast feeding women,473 infants and 502 young children were monitored,and the median value of urinary iodine was 129.3-189.6,114.6-190.6,148.5-298.5 and 144.4-187.3 μg/L,respectively.A total of 1657 urine samples were monitored,1264 intelligence quotient level and 1197 thyroid volume of school-age children were determined,the median urinary iodine,thyroid goiter rate and intelligence quotient level was 217.9-266.7 μg/L,0-3.29％ and 110.4-117.2 μg/L,respectively.Conclusions From 2006 to 2010,the city's households qualified iodized salt coverage rate has reached the national standard for elimination of iodine deficiency disorders.The iodine nutrition of key population and school-age children has reached adequate level,the thyroid goiter rate is less than 5％,and the level of intelligence quotient has increased every year.But the iodine nutrition of breast feeding women in 2009 and 2010,pregnant women in 2010 is inadequate,so iodine nutrition surveillance and health education in pregnant and breast feeding women need to be strengthened in the future.

Objective To assess the feasibility of ~(18)F-FDG PET/CT for differentiation of malignancy from benign in pericardial Lesions. Methods ~(18)F-FDG PET/CT data of 16 patients with pericardial lesions (9 malignancy, 7 benign) were analyzed using maximum standard uptake values (SUV_(max)) and the density of lesions, the relationship with surrounding tissues were also evaluated. The results of malignancy or benign were proved by needle biopsy.Results SUV_(max) of malignancy (10.1±6.0) and benign (3.0±2.1) was significant different (P<0.01). Taking the optimal cut-off value as SUV_(max) 3.5-4.5, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value of differentiation of malignant from benign in pericardial lesions was 100%, 85.71%, 93.75%, 90.00% and 100%, respectively. Conclusion ~(18)F-FDG PET/CT can predict benign or malignance of pericardial lesions with moderate accuracy.