On the basis of randomized clinical trials, the extended (D2) lymph node dissection is now recommended as a globally stan-dard procedure for locally advanced gastric cancer. However, the rational extent of lymphadenectomy for locally advanced gastric can-cer has remained a topic of debate in the past decades. The examined lymph node and extra-nodal metastasis are significantly associ-ated with the survival of gastric cancer patients. Furthermore, the role of splenectomy for complete resection of No. 10 nodes has been controversial;however, the randomized trial of JCOG0110 is yet to be completed. Gastric cancer with No. 14 lymph node metas-tasis is defined as M1 stage in the current version of the Japanese classification. We propose that D2+No.14v lymphadenectomy may be an option in a potentially curative gastrectomy for tumors with apparent metastasis to the No. 6 nodes or infiltrate to duodenum. In view of the limitation of low metastatic rate in para-aortic lymph nodes in Japan Clinical Oncology Group (JCOG9501), the clinical benefits of D2+PAND for patients with stage T3 and/or stage N3 disease, both of which are very common in China and many other coun-tries except Japan and Korea, cannot be determined.

Digestive tract reconstruction after distal gastrectomy for gastric cancer includes Billroth Ⅰ (B Ⅰ),Billroth Ⅱ (B Ⅱ),Roux-en-Y (RY),uncut-RY,RY-double tract (DT) and jejunal interposition (JI).B Ⅰ reconstruction is the most common method,with an advantage of keeping normal duodenal pathway for food.The disadvantage of B Ⅱ reconstruction is that it could cause dumping syndrome-related syndroms compared with RY reconstruction.RY reconstruction was not superior to B Ⅰ reconstruction in terms of keeping body weight and improving nutritional status,although it could significantly decrease the incidences of reflux residual gastritis and reflux esophagitis.Uncut-RY reconstruction is better than Roux-en-Y reconstruction in the prevention of Roux stasis syndrome.DT reconstruction has not only the advantages of descending the incidences of reflux residual gastritis and reflux esophagitis but also kept the normal duodenal pathway for food.JI is feasible and safe with the advantages as mentioned above,however,it has complicated surgical process and time-consuming,and anastomotic ulcer may occur after the surgery.

D2 radical lymphadenectomy is currently the worldwide standard operation for locally advanced gastric cancer. The most common organs affected by intraoperative injuries include perigastric blood vessels, the spleen, the common bile duct, the pancre-as, and lymphatic vessels. Postoperative complications usually include bleeding, lymphatic leakage, anastomotic leakage, and delayed gastric emptying. Annual volume is an important factor in the mortality and morbidity of the operation. The learning curve for the proce-dure involves approximately 50 operations. Adequate living anatomical knowledge and skilled surgical techniques are prerequisites for D2 lymphadenectomy.

The rational extent of lymphadenectomy for local advanced gastric cancer is a controversial issue in the past decades.D2 radical gastrectomy is recommended as a worldwide standard procedure for local advanced gastric cancer based on the international publications of randomized clinical trials.Because of the limitations of design in the randomized clinical trial (JCOG9501),the significance of No.16 lymph node dissection for patients with T4 and N3 stage of gastric cancer which is very common in China is not very clear.The clinical efficacies of splenectomy for complete resection of No.10 lymph node need to be confirmed by the final result of the randomized clinical trial (JCOG0110).Although positive No.14v and No.13 lymph nodes metastasis are defined as distal metastasis (M1) according to the Japanese gastric cancer treatment guidelines 2010 (ver.3),D2 radical gastrectomy plus No.14v and No.13 lymphadenectomy should be applied to the potential patients with positive No.6 lymph node metastasis or distal advanced gastric cancer with duodenal invasion.The number of lymph node dissection and extra-nodal soft tissue dissection are significantly associated with the prognosis of patients.

Gastrointestinal stromal tumor (GIST) arises from gastrointestinal tract,omentum,mesentery or peritoneal surface.Of which,about 60％ arises from stomach.The principle of surgical treatment is removing the tumor as completely as possible.The indications of operation for metastatic and recurrent GIST are perforation,obstruction and hemorrhage.The majority of gastric stromal tumor can be removed with local or wedge excision.Proximal gastrectomy is a choice for GIST locating at the cardia since local resection may cause cardia stenosis.Distal gastrectomy is suitable for GIST locating at lesser curvature of gastric antrum.Total gastrectomy is not a common procedure for gastric stromal tumor.Combined spleen,tail of pancreas and transverse colon resection may necessary for R0 surgery.Local resection is the first choice for duodenal stromal tumor,pancreaticoduodenectomy can be performed in large medical center.Operative procedure must be carefully carried out for small intestinal stromal tumor to avoid tumor rupture.Combined resection is a right choice when tumor involved surrounding organs or structures.In principle,low anterior instead of abdominoperineal resection is the only reconmend procedure for rectal stromal tumor.

Objective To introduce a new anus preserving operation for low rectal cancer-ultralow anterior resection and colorectal/coloanal anastomosis by using supporting-bundling up method.Methods The clinical data of 310 patients who underwent anus preserving operation by supporting-bundling up method for low rectal cancer were retrospectively reviewed.Results The mean distance of tumor from the anal verge was 4.7?1.2cm;the TNM stage was stage Ⅰin 40 cases,stage Ⅱ30 cases,stage Ⅲ109 cases and stage Ⅳ 31 cases.the mean distance of the anastomosis from the anal verge was 2cm(1-4cm).the occurrence rate of postoperative anastomosis leak was 2.3%,the rate of excellent anal sphincter control was 82.25%.The 5-year local recurcence rate was 5.8%.Conclusions Ultralow anterior resection and colorectal/coloanal anastomosis by supporting-bundling up method may be one of the best anus preserving operations for low rectal cancer.It is a safe and feasible operation,and the long-term outcome is excellent.

With the development of peaple's life and the change of diet,the incidence rate of colorectal cancer is increasing. There are Twenty-five percent of patients were found liver metastases in the first diago-sis. Surgical resection of liver metastases from colorectal cancer is known to be associated with long term sur-vival. So it is the key to increase the resection rate for colorectal cancer patients with liver metastases. The in-curruence of neoadjuvant therapy may be useful in therapy of colorectal cancer patients with liver mtastases.

Metabolic components of human intestinal flora bind to their corresponding receptors and stimulate inflammatory cy-tokine secretion. Other changes become evident and cause inflammation, as a result, colorectal cancer (CRC) occurs. Probiotics protect intestinal mucosa and prevent CRC by functioning as an intestinal barrier and inhibiting DNA damage. Probiotics can also be used not only to prevent CRC but also to induce adjuvant treatment of CRC. Gastrointestinal tract surgery can affect gut microbiota metabolism and microecological balance. This review focuses on current research progress on the relationship between intestinal microflora and CRC.

Drug abuse and addiction are increasingly severe, which is both a serious social problem and an important medical research work. It is very significant to evaluate dependence potential of centrally acting drugs. Several lines of evidence have shown that there is a close relationship between drug sensitization and drug abuse and addiction. More attention has been directed to sensitization of abused drugs. We introduce the establishment of behavioral sensitization animal model, review neurobiological mechanisms underlying development and expression of behavioral sensitization, and further discuss the role of behavioral sensitization animal model in evaluation of drug dependence.

Objective To explore the effect of heroin dependence on the expression and the function of glucagon in islet A cells of rats.Methods The changes of numerical density on area of A cells and level of plasma glucagon of heroin-dependent rats were investigated by means of the immunohistochemical SABC method,image analysis and radioimmunoassay(RIA).Results As compared with SCG and NCG,it was observed that the number of Glu-IR positive cells increased and the intensity of immunohistochemical staining enhanced in the heroin-dependent groups.The result of image analysis showed that the numerical density on area of A cell apparently increased in the 38-day heroin-dependent group(P