Aged ; Female ; Humans ; Male ; Middle Aged ; Silicosis

Homeostasis between the host and viruses is naturally maintained.On the one hand,the immune system activates the immune re-sponse to kill or eliminate viruses;on the other hand,the immune system controls the immune response to maintain immune homeostasis. The cause of persistent infections with hepatitis viruses such as HBV and HCV is that viral molecules damage the immune system of the host and their variants escape immune clearance.Long-term coexistence of the host and viruses is the process involving various immune cells and molecules and is the result of homeostasis maintenance in antiviral immune response.The immune homeostasis maintained during persis-tent infections with hepatitis viruses is analyzed by the cellular and molecular mechanisms.

Objectives To understand the susceptible factors for tobacco use among doctors in Henan province.Methods A random multilevel cluster sampling method was conducted.2 990 subjects were studied with an inquiry and self-reported questionnaire.Results The total smoking rate was 27.5%.The current smoking rate was 22.3%.Daily smoking rate was 13.3%.The main reasons for smoking were social needs,pressure relief,getting refreshing.About 21.4% subjects smoked in front of patients.Majority doctors understood the relationship between smoking and some diseases.The multivariate analysis showed that the doctors'smoking behavior was related with his gender,age,education background,monthly revenue,working section,hospital administration level,knowledge scale of tobacco being harmful on health,knowledge scale on the relationship between smoking and illness,doctors'social norm attitude for smoking.Conclusions The smoking behavior of doctors was complex,which was related to personal,social environment factors.Only comprehensive smoking prevention interventions could be stressed to produce lasting behavior change.

Objective To construct the endothelial cell model with overexpressed human protein kinase C ?2(PKC?2) after high glucose inducement in order to study the function of human PKC?2. Methods The PRKCB1 gene was amplified from pMD18-T-PRKCB1 plasmid and then directly cloned into shuttle plasmid pDC315 to construct shuttle plasmid. Then the recombinant shuttle plasmid and adenovirus genomic plasmid pBHGlox△E1,3Cre were cotransfected into 293 cells to construct recombinant adenovirus Ad-PRKCB1. The virus titer was calculated by TCID50. The Ad-PRKCB1 was verified by immunocytochemistry and RT-PCR. Ad-PRKCB1 was transfected into human umbilical vein endothelial cells (HUVECs) followed by the treatment of high glucose (25 mmol/L) for 96 h, and then the cells were observed by laser scanning confocal microscopy (LSCM). Results Restrictive endonuclease digestion and PCR result showed that the target gene was correctly cloned into shuttle plasmid. Cytopathic effect (CPE) was observed under inverted microscope through homologous recombination. The virus titer was 7.9?109 IU/ml. Immunocytochemical staining and RT-PCR indicated the expression of human PKC?2 in the transfected HUVECs. The translocation was observed under LSCM. Conclusion A recombinant adenovirus vector with human PKC?2 and the endothelial cell model with human PKC?2 overexpression by high glucose are constructed successfully.

Objective: To establish a new method for the identification of Schisandra chinensis(Turcz.) Baill. (Beiwuwei) and Schisandra sphenanthera Rehd. Et Wils. (Nanwuwei). Methods: Random amplified polymorphic DNA method was applied to screen random primers. Results:Screening from 80 primers,only S429, which can be used to identify Schisandra chinensis(Turcz.) Baill.(Beiwuwei) and Schisandra sphenanthera Rehd. Et Wils. (Nanwuwei) accurately and is of good reproducibility. Conclusion: S429 can be used to identify Schisandra chinensis(Turcz.) Baill. (Beiwuwei) and Schisandra sphenanthera Rehd. Et Wils. (Nanwuwei) accurately.

Objective To detect the expression of RXR? gene protein in gastric cancer, superficial gastritis, normal gastric mucosa, in order to explore the relationship between the development of gastric cancer, combined with clinical and pathological analysis of its clinical significance. Methods Using immunohistochemical detection RXR? protein in gastric cancer, superficial gastritis, gastric mucosa in the normal expression. Results RXR? protein mainly located in the nucleus of cells, there are a small number of cytoplasmic staining, a brown or yellow granular, in gastric cancer, superficial gastritis, normal gastric mucosa, RXR? protein expression rates were 25.0%, 81.1%, 96.1%; group and the normal mucosa and superficial gastritis, gastric cancer group RXR? expression rate has dropped, there were significant differences (P0.05). The lymph node metastasis and tumor pathology and clinical phases closely related (P

Objective To study the inhibition of HCV IRES mediated HCV core protein expression in cells by inhibitor RNA. Methods Plasmid pcRz-IRNA, a eukaryotic expression vector with IRNA and two self cleavage ribozyme overhang at both sides respectively, was constructed and co-transfected with pcHCVcluc (containing HCV NCR, core and Luc genome) into the HHCC cell line (Human Hepatocellular Carcinoma cell line). Immunoflurescence tests were applied to detect the co-transfected cells, which were thereafter analysed with confocal microscope quantitatively. Luciferase activity was valued using Luc Assay System (Promega). Results The cotransfected cells expressed HCV core protein, and the fluorecein in which was reduced significantly in comparison with control. Conclusions IRNA can inhibit the expression of HCV IRES mediated core protein in the cotransfected cells.

Aim To investigate the Clotrimazoles (CLT) inhibitive effect on the secretion of ?-defensins of the Paneth cells.Methods The ?-defensin mRNA expression is determined by semi-quantitative RT-PCR. The secretion of defensins is determined by dot-blot and ELISA. Results CLT has no influence on the defensin mRNA expression. All of 125 nmol?L -1, 250 nmol?L -1 and 500 nmol?L -1 CLT can inhibit the secretion of ?-defensins. Conclusion CLT could inhibit the secretion of defensins, which suggests that CLT maybe influence the function of small intestine

Objective To investigate the effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 (VCAM-1) in human vascular smooth muscle cells (VSMCs) induced by angiotensin Ⅱ (AngⅡ). Methods The fourth to sixth passages of in vitro cultured VSMCs from human umbilical artery were used. The cells were divided into six groups, i.e. control group, AngⅡ (1?10 -6mol/L) group, Ang Ⅱ with exposure to 1, 5, 10 or 50 Gs of constant magnetic field groups. Samples were collected 12 hours after intervention. The secretion of VCAM-1 was assayed by enzyme linked immunosorbent assay (ELISA) and the expression of VCAM-1 was assessed by immunocytochemistry. Results The secretion of VCAM-1 was increased significantly 12 hours after intervention with AngⅡ of 10 -6mol/L (P

Objective:To summarize the allergic mechanism caused by traditional Chinese medicine injection (TCMI) to provide some references for perfecting Good Laboratory Practice(GLP) of TCMI.Method:The related iteratures in data bases at home and abroad were reviewed,and the present experimental research methods about allergies were referred to to have a view of future studies.Result:The allergic mechanism of TCMI was mostly antigen-antibody reaction, part of which was anaphylactoid reaction.The method of the evaluation of allergy caused by TCMI only was animal experiments, but there were still some allergies caused by TCMI after the evaluation with this method.The present experimental research methods indicated that the detection of mediators of inflammation and FCM(Flow Cytometry) could be used to evaluate the allergies caused by TCMI.Conclusion:More attention should be paid to allergies caused by TCMI for its complicated mechanism and frequent occurrences in clinic.It may be an effective way to evaluate the allergies caused by TCMI with several methods including in vivo and in vitro.