1.Quality Standard for Huisheng Diyidan Tablet
Chinese Traditional Patent Medicine 1992;0(02):-
AIM:To establish the method for quality control of Huisheng Diyidan Tablet(Eupolyphagea seu Steleophaga;Radix Angelicae sinensis,Sanguis Draconis,etc.). METHODS: Radix Angelicae sinesis,Eupolyphaga seu Steleophaga,Chinnabaris,Moschus,Boswellia carterii were identified by microscope.Sanguis Draconis was identified by TLC,Pyritum was identified by general identified reaction;The content limitation of cinnabaris(HgS) was determined by titration.Ferulic acid content of Radix Angelicae sinensis was determined by HPLC. RESULTS: Every part of the tablet was identified,the content limitation of cinnabaris(HgS) and ferulic acid content of Radix Angelicae sinensis were determined. CONCLUSION: The method showed that the characteristic for identification is distinct and highly specific,quantitative assay is simple,accurate,reliable and can be used for quality control of Huisheng Diyidan Tablet effectively.
2.3 Cases of Nose Desease
Journal of Zhejiang Chinese Medical University 2006;0(05):-
Teacher Lian Jianwei is good at treating internal diseases,however he is experienced at nose diseases too by differentiating signs and looking into inside from outside;now 3 medical concerned cases are extracted for reference.
3.Progress in diagnosis and treatment of acute kidney injury post neonatal asphyxia
International Journal of Pediatrics 2012;39(1):36-39
Acute kidney injury(AKI) is a common consequence of perinatal asphyxia,occurring in up to 56% of these infants,which can cause multiply organ dysfunction and neonatal death.This review summaries the development of early detection indexes in laboratory and clinic such as novel biomarker,urinary protein,urinary enzyme,renal blood flow velocity by pulse Doppler ultrasonography,and treatment in AKI post neonatal asphyxia.It provides more positive meanings on early detection,early management and eventually mortality reduction.
4.My viewpoint on cleft lip and palate treatment.
Chinese Journal of Stomatology 2010;45(10):577-580
Cleft Lip
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therapy
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Cleft Palate
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therapy
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Humans
6.SERUM CONTENT OF B2 MICROGLOBULIN IN VIRAL HEPATITIS PATIENTS
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
?2 Microglobulin levels were measured by radioimmunoassay in the serum of 114 patients with viral hepatitis and asymptomatic HBsAg carriers, and compared with 24 normal controls.Elevated serum ?2 microglobulin levels were found in acute viral hepatitis, chronic persistent or active hepatitis. The quantity of the serum ?2 microglobulin was remarkably increased in the chronic active hepatitis (P
7.A Comparative Study of the Expressions of ets-2, IGF-Ⅱ, C-myc and N-ras in Human Primary Hepatocelluiar Carcinoma and Tumor-adjacent Tissues
Academic Journal of Second Military Medical University 1982;0(01):-
The expressions of ets-2, IGF-Ⅱ, C-myc and N-ras in 12 pairs of human primary hepatocellular carcinoma (PHC) and tumor - adjacent tissues are presented in the present paper. The results showed that there was at least one of the four oncogenes studied overexpressed in the 12 pairs of samples. ets -2 was the most commonly expressed oncogene seen in all the PHC and tumor - adjacent tissues, with 3. 5 and 2.4 kb as the major two bands, which differed from the evenly expressed 4. 5, 3. 5 and 2. 4 kb bands in the normal control livers. In six tumor-adjacent tissues the expression of ets-2 was higher than that in PHC. IGF-Ⅱ was expressed as 5. 0 and 2. 0 kb fetal transcripts in PHC and tumor-adjacent tissues, while in the normal control livers it was 5. 6 kb. One tumor-adjacent tissue had IGF-Ⅱ fetal transcripts expressed but the corresponding PHC had no IGF -Ⅱexpressed, indicating the reverse differentiation in PHC. N-ras was expressed as 4. 0 kb band in 8 out of 12 PHC and in 6 out of 12 tumor-adjacent tissues. In two cases the expression of N-ras was higher in tumor-adjacent tissues than in PHC. 5. 6 and 2. 6 kb N -ras transcripts were also detected in one pair of PHC and tumor -adjacent tissue and in two tumor - adjacent tissues, together with the 4.0 kb transcript. C - myc was expressed as 4. 0 kb band in 9 out of 12 PHC and in 6 out of 12 tumor-adjacent tissues. One tumor-adjacent tissue had higher C-myc expression than PHC. In two PHC 2. 2 kb C-myc transcript was detected besides the 4.0 kb transcript. The roles and relationships of these oncogenes in the carcinoge-nesis of human PHC are discussed in detail.
8.The States of HBV in Human Primary Hepatoceilular Carcinoma and Tumor-adjacent Tissues: A Comparative Study
Academic Journal of Second Military Medical University 1981;0(03):-
The present paper reports on the study of HBV states in human primary hepatocellular carcinoma (PHC) and tumor-adjacent tissues. The results showed that in 11/12 PHC (91.5%) and 9/12 tumor-adjacent tissues (75%) there were integrated forms of HBV DNA. The amount of HBV integrated in PHC was higher than that in tumor-adjacent tissues, indicating a clonal selection for liver cells containing integrated HBV during the malignant transformation of PHC. In two tumor-adjacent tissues only free HBV was found, while in the corresponding PHCs there was integrated HBV DNA. This suggests that integration had taken place in the process of carcinogenesis of PHC. It was also observed in this study that four patients had putatively defective HBV in their liver cells which could be replicated but not be secreted into serum. The possible role of HBV in the carcinogenesis of PHC is discussed
9.Cloning and sequencing analysis of human PKC?Ⅱgene ORF
Journal of Chongqing Medical University 1987;0(01):-
Objective:To clone human PRKCB1 gene open reading frame(ORF) in order to further research on it's function.Methods:RT-nested PCR method was adopted to the amplify the total length of human PRKCB1 ORF from human umbilical vein endothelial cell(HUVEC).T vector was inserted into the harvested fragment after a tail was added.With blue white screening,the gene ORF encoding human PKC?Ⅱ was obtained by special primers amplifying recombinant plasmids,and linked into T vector.Then the plasmid was identified by sequencing.Results:The human PRKCB1 ORF was amplified successfully with RT-nested PCR and T/A cloning,and the gene sequence was completely consistent with that reported in GenBank.Conclusion:Human PRKCB1 gene ORF was successfully cloned.The strategy of cloning may provide technical references for some genes hard to be cloned.
10.Progress in genetic basis of congenital heart disease
Chinese Journal of Applied Clinical Pediatrics 2016;31(13):969-972
Congenital heart disease (CHD)is the most common human congenital malformation,which is the biggest cause of infant morbidity and mortality.Even though it can be corrected by surgical operation,the patient also faces a variety of surgical complications and even the risk of disease recurrence,which brings huge economic pressure to the society.At present,the occurrence of CHD is related to the gene mutation,chromosome variation,environmental fac-tors and epigenetic inheritance.This paper focuses on the genetic basis of CHD and genetic testing technology,to deepen the understanding of the genetic basis of the disease,to provide the basis for the prevention of disease and early diagnosis.