Child ; Female ; Humans ; Job Syndrome

Endophytic fungi which reside in the tissue of mangrove plants seem to play an important role in the discovery of new biologically active substances. During the course of screening for the antimicrobial metabolites from the endophytic fugus Penicillium sp. FJ-1 of mangrove plant Avicennia marina, a new aurone glycoside (1) was isolated by repeated column chromatography on silica gel and recrystallization methods. The structure of 1 was elucidated as (Z)-7,4'-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside, on the basis of spectroscopic analysis. Compound 1 exhibited antifungal activity against Candida sp., with the potency comparable to amphotericin B and much better than fluconazole. Compound 1 can also inhibit extracellular phospholipase secretion in a concentration-dependent manner.
Antifungal Agents ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Benzofurans ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Candida ; drug effects ; Glycosides ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Molecular Structure ; Penicillium ; chemistry ; genetics ; isolation & purification ; metabolism ; Seawater ; microbiology

Objective: To express and purify transforming growth factor ?(TGF?)-pseudomonas exotoxin 40 and investigate its cytotoxic effect on cancer cells overexpressing epidermal growth factor (EGF) receptor. Methods: Recombi nant plasmid pV28 was constructed by inserting the gene coding TGF?-PE40 into the vector pET28a Expression of fusion protein was conducted using the host BL21. Production of the recombinant protein was induced by IPTG, following extraction and purification of inclusion bodies with His-tag purification system. Cell viability assay (by MTT) was performed to determine the cytotoxic effect of TGF?-PE40 on cancer cells ( A431 and SK-OV3). Results: Recombinant plasmid pV28, which expresses TGF?-PE40, was constructed successfully. Purity of TGF?-PE40 was about 98% after a purification procedure using His-tag column. Cytotoxic experiment showed that at a concentration of 0. 86?0. 07 UUUUg/ml, TGF?-PE40 could reduce 50% viability of A431, which has high expression of EGFR. Whereas the IC50 for ovarian cancer cell SK OV3, which expresses less EGFR, was 6.37?2.18 ?g/ml. There was a significant difference between these two groups (P

Objective To ascertain the dose levels to personnel from the activation products in the radiotherapy place of a proton acceterator and to provide technical basis for protection design of the treatment place and the personnel's radiation protection.Methods A PROTEUS 235 proton therapy system was analyzed for the potentials to produce activated nuclides in the air during its operation.The concentrations of the main radionuclides and the resultant personnel doses were estimated.Results The activity concentrations of activated nuclides were 15O 4.1 × 10-2,13N 3.3 × 10-2,41 Ar 6.2 × 10-3 and 11 C 2.0 × 10-2 Bq · cm-3,respectively,in proton accelerator hall,whereas those in the proton beam radiotherapy room were only about 5％ of the corresponding values in the proton accelerator hall.The annual doses to the radiation workers from immersion external exposure due to air activation were less than 1 and 0.1 mSv,respectively,when entering the proton accelerator hall 5 and 30 min after beam stopping;whereas those were 1.3-2.0,0.7-1.2 and 0.3-0.6 mSv,respectively,when entering the therapy hall 0,5 and 15 min after beam stopping.Conclusions The personnel doses received from activated nuclides in the air in the radiotherapy place of a proton acceterator are unignorable.The contribution to doses from immersed external exposure of 15O,13N,41Ar and 11C should be taken into full considerations,with necessary protection measures needed.

Objective To explore the effects of PSE on hemodynamics and Liver function in LCPH. Methods 30 patients with LCPH are treated by PSE. The hemodynamics changes of patients with LCPH are evaluated with color Doppler diasonograph. The changes of main clinical features which is related to LCPH , serum ALT and albumn were investigated . Results The hemodynamics indexs of LCPH are all changed significantly (P0. 05) between four and two weeks after PSE. But the main symptoms, signs of LCPH and liver function are improved after PSE . Conclutions PSE plays an important role in hemdynamics and liver function of LCPH. After PSE, Portal hypertension decreases effectively and liver function is improved.

Isopinosine (ISO, 25?100 mg/kg?d-1ip for 4 or 5d) significantly increased the production of serum hemolysin-and spleen plaque forming cell ( PFC) in normal mice immunized with SRBC. The same result was observed in immunosuppressive mice induced by cy clophospha-mide. In 16 month aged mice, ISO (ip for 5 d) showed potentiating effect on anti-SRBC PFC response at the dose of 2 .5 mg/kg, no significant effect at the dose of 5 .0 mg/kg, and inhibiting effect at the dose of 25 and 100 mg/kg respectively. 1SO(25,50 and 100 mg/kg?d-1 ip for 9 or 10 d ) remarkably enhanced delayed cutaneous hypersensiti-vity (DCH) to 2, 4 -dinitrochlorobenzene (DNCB) in normal mice as well as in immunosuppressive mice induced by cyclophospha-mide. ISO (5, 10 and 20 mg/L ) signficantly increased ConA-induced lymphocyte proliferation of spleen cells from C57BL/6J mice in vitro. There was also potentiating effect on lymphocyte proliferation induced by ConA in 16 month aged mice.

Objective To improve the implatation of silicone prosthesis for rhinoplasty.Methods Forty one nasal specimens of cadavers were studied with anatomical and histological methods. And 213 cases of rhinoplasty via the retrofasial space (RFS) of dorsum nasi were performed.Results The upper extremity of RFS looked like bifurcation shape. Such surgery was performed for implatation by silicone prosthesis with V shape notch, and the rhinoplasty was successful with satisfactory effects.Conclusion Improved implatation of silicone prosthesis is an effective method for rhinoplasty.

We reported a case of children's OSAHS caused by the huge fibrolipoma in pharynx nasalis. The patient was a 10-years-old child who went to the hospital with the chief complaint of "Snoring and mouth breathing during sleep for 10 years". Imaging tests found one huge tumor in pharynx nasalis before the operation. The tumor was resected totally. The postoperative pathological diagnosis was fibrolipoma. No recurrence was noted during the follow-up visit one year postoperatively. The clinical features, diagnosis, treatment, pathology and prognosis were reviewed herein.
Child ; Humans ; Lipoma ; complications ; surgery ; Neoplasm Recurrence, Local ; Pharynx ; pathology ; Prognosis ; Sleep Apnea, Obstructive ; etiology ; Snoring

BACKGROUND:The inflammatory cytokines,produced by synovial cells and invaded monocytes/macrophages and lymphocytes in synovial tissues of rheumatoid arthritis(RA),play a major role in rheumatoid synovial pathology.Interleukin(IL)-17,a recently found inflammatory cytokine,associates with many autoimmune diseases.OBJECTIVE:To detect IL-17 level Levels of interleukin-17 in serum of rats with collagen-induced arthritis(CIA),to study the relationships among IL-17 and the arthritis index,IL-1?,tumor n?ecrosis factor?(TNF-?),as well as matrix meralloproteinase 3(MMP-3).DESIGN,TIME AND SETTING:A randomized controlled animal trial was performed in the Immunology Laboratory of Medical College of Dalian University from March to August 2008.MATERIALS:Fifty female,inbred Wistar rats,aged 4-6 weeks,with average weight of(180?15) g.METHODS:Forty out of 50 rats were randomly selected for establishing CIA models.The other 10 rats were regarded as the normal control group.Rats in CIA group were injected intradermally 200 ?L emulsified type-II collagen at the base of the tail after model preparation.At day 7,a second boosted injection of emulsified CII in 100 ?L was administered at the base of the tail.The rats in normal controls were injected with the same dose of saline.MAIN OUTCOME MEASURES:Paw swelling was observed at 24 hours after immunization;The levels of IL-17,IL-1?,?T?NF-? a?nd MMP-3 were examined by ELISA;The inflammatory index of CIA rats was observed.RESULTS:The levels of IL-17,IL-1?,TNF-?,as well as MMP-3 in the CIA group was greater than that of the control group(all P

Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a common clinical disease that seriously threatens human health and life. Accurate location of the upper airway obstruction is the key to the diagnosis and treatment of OSAHS. Acoustic pharyngometry uses sound reflection to quickly assess the cross-sectional area and volume of the upper airway. Acoustic pharyngometry represents a simple, quick, non-invasive method for measuring upper airway dimensions which could predict sleep apnea risk. In this article we sought to introduce the application of acoustic pharyngometry in the diagnosis and treatment of OSAHS.
Acoustics ; Humans ; Larynx ; Pharynx ; diagnostic imaging ; Sleep Apnea, Obstructive ; diagnosis ; therapy ; Syndrome