OBJECTIVE To explore the rational application of aminoglycosides antibiotics.METHODS We randomly selected 520 patients' records of applying aminoglycosides antibiotics from Jan to Dec in 2007.Types of antibiotics,dosages,usage,ways of delivery and periods and combination of medicines were analyzed.RESULTS Out of the 520 cases,the oldest patient was 89 year and the youngest one was 7 years.The application rate of amikacin was the highest(35.6%),which was delivered mainly by iv,the combination of medicines accounted for 92.7%.CONCLUSIONS The application of aminoglycosides antibiotics in our hospital is basically rational.In some cases the improper antibiotics are used and the duration is a little longer,which should be supervised.

According to the registered product standards specification of medical devices, combined with the standard reviewing work, the common problems of standards during medical devices registration were analyzed and corresponding suggestions were proposed to standardize the standard of the registered product, accelerate the standardization and promote the industry standardized.
Device Approval ; Equipment and Supplies ; standards

Objective To develop novel disposable liquid filling device as well as its function and clinical value. Methods The experimental group adopted disposable liquid filling device,while the control group used injector simply.The result underwent comparison analysis,including time cost for liquid preparation,pollution of medical sollution by bacteria and particles and spattering of medical sol-lution. Results Disposable liquid filling device shortened time cost for liquid preparation,reduced pollution of medical sollution by bacteria and particles and prevented spattering of medical sellution. Condusions Disposable liquid filling device possesses many characteristics such as simple structure,low cost, easy operation,high efficiency,reduction of medical sollution pollution and prevention of spattering.It is worthy of clinical application.

Objective To investigate the related factors of recurrence of early gastric cancer (EGC) after endoscopic resection. Methods Clinicopathologic data of 169 patients with EGC who underwent endoscopic resection and periodically followed up by the Chinese PLA General hospital were analyzed retrospectively. Results During a follow-up of 13-57 months (median time 24. 5 months), 12patients had gastric cancer again and the recurrence rate was 7. 1% (12/169). The recurrence time varied from 3 to 36 (28 ± 23)months and the median time was 18 months. The recurrence rates of 0.5 year, 1st year, 2nd year and 3rd year were 1.18% (2/169), 3.55% (6/169), 9.91% (11/111) and 12.24%(12/98), respectively. Eleven patients had gastric cancer again within 2 years after resection.Undifferentiated histology (including poorly differentiated carcinoma and signet ring cell carcinoma),submucosal infiltration and lymphovascular invasion of the primary lesion of EGC were related to thepostsurgical recurrence ( all P ＜ 0. 05). Conclusion Most recurrence of EGC occurred within 2 years afterendoscopic resecton and is related with undifferentiated histology, submucosal infiltration andlymphovascular invasion. It is important for these patients to receive endoscopy follow up.

Objective To investigate the value of the senile degenerative aortic valve calcification (AVC) and to analyze the correlative risk factors by color Doppler echocardiograph.Methods Aortic valves of 110 cases with AVC (study group) and 110 cases without AVC (control group) were observed by color Doppler echocardiograph.All the patients with AVC have been excluded of congenital and other acquired valves' diseases.The aortic valve' s shape and the diameters of aortic root were measured.At the same time,the peak systolic and diastolic velocities of aorta were detected.The different features were compared between the two groups.Results Test results showed significant differences in study group:Anteroposterior diameter of aortic annulus and the area of aortic valve were decreased by calcification; The anteroposterior diameter of aortic sinus increased and aorta transvalvular pressure gradient augmented; Detection rate of atherosclerosis (AS) of aorta and the peripheral artery stenosis of the study group were higher than that of control group; The AS increased pulse pressure and aggravated AVC; Correlative risk factors,in addition to age,included hypertension,hypercholesterolemia,osteoporosis,and so on.The correct diagnosis of the correlated diseases were on the basis of clinical examines.The rate of AVC with multi-factor was higher than that with single-factor.In addition,gender difference and the diameters of ascending aortic showed no significant difference in two groups.Relationship between diabetes of correlative factors and the rate of AVC showed no significant difference in two groups.Conclusion Morphologic abnormality and malfunction of aortic valve and the large artery in elder patients were caused by senile degenerative AVC.The more correlative risk factors,the worse showed.

Based on M9 culture medium,the concentration of ingredients of culture medium was optimized for the fermentation of pNSR32/BL21(DE3),the engineering bacterial with spider silk protein,and lactose as an inducer.The condition of optimum culture medium was obtained for the expression of the high molecular weight recombinant spider silk protein by using orthogonal and individual factor experimental design.The result was showed that the optimum culture medium was consisted of 0.3% glycerol,3% yeast,0.75% tryptone,0.05%(NH_4)_2SO_4 and a little inorganic salt_.It was confirmed that the optimum culture medium will benefit the growth of bacterial and expression of recombinant spider silk protein.The production level of propose protein will attain at 20% of the total proteins in the fermentation.

Objective:To investigate the potential of differentiating into myocytes of the granulocyte colony stimulating factor(G CSF) and Simvastatin mobilized bone marrow stem cells. Methods:After injection of isoprenaline(ISO) interaperitoneally to develop acute ischemic model, rat bone marrow stem cells were mobilized by G CSF and dextran sulfate sodium salt and migrated to the site of myocardial infarction. Hearts were harvested from 24 hours to 24 weeks after administration of ISO for histopathological examination.Immunohistochemisty?HE and VG stain were used to detect infiltration of CD34 + monocytes and the regeneration of myocytes and the inhibition of fibroatrophy of ischemic myocardium.Results:24 hours after administration of ISO,a large amount of infiltrative monocytes and regenerative myocytes which were CD34 positive expression could be found in the infarct zones of the G CSF treatment group,while majority of the infiltrative inflammatory cells in control group were neutrophils and there was no infiltrative cells and myocytes which were CD34 positive expression,4 weeks after administration of ISO,there were a plenty of scar in control group,but not in the treatment group.Conclusion:Rat bone marrow stem cells were mobilized by G CSF and Simvastatin,and migrated to the site of myocardial infarction,and differentiated into viable cardiomyocytes or vascular endothelial.The heart function remarkably was improved by regeneration of myocytes,inhibition of fibroatrophy and protection of ischemic myocardial structure.

Objective To analyze the detection results of 75 suspected type A influenza cases and to understand the distribution situation of influenza patients to provide the guidance for its early prevention and control .Methods The nasopharyngeal swabs specimens from 75 patients with suspected type A influenza were detected for understanding the viral infection situation by real‐time fluorescent quantitative PCR .Results In the specimens of 75 suspected patients ,13 cases with type A influenza virus were detected out ,in which 9 cases were the H1N1 subtype ,mean age > 50 years old .The temporal distribution was 3 cases in March 2013 ,1 case in April ,7 cases in January 2014 and 2 cases in February .No H7N9 subtype was detected out .Conclusion Real‐time fluores‐cent quantitative PCR has the characteristics of rapidness and high accuracy .The winter is the high onset season of flu ,elderly pa‐tients are susceptible to influenza virus .The monitoring and early treatment should be strengthened .

Objective To investigate the effects of pyrrolidine dithiocarbamate(PDTC) on aquaporin-9(AQP9) expression and brain edema around the hematoma in experimental intracerebral hemorrhage(ICH).Methods 128 SD rats were randomly divided into four groups: normal group,control group,ICH group and PDTC group.According to Rosenberg's methods,the models of experimental ICH were established.The rats in PDTC group were received PDTC intraperitoneally 2 h later.The brain weight column(BWC) at different time point was calculated by gray-wet method,and the expression of AQP9 was examined by immunohistochemistry.Results The BWC and expression of AQP9 in hematoma side of ICH begin to increase 4 h after ICH,peaked at 72 h,and decreased 120 h later.The BWC in opposite side of hematoma also increased 4 h after ICH and peaked at 72 h.Compared with control group,the BWC and expression of AQP9 in ICH group were higher,especially in hematoma side of brain(all(P

Objective To study the correlation between senile mild cognitive impairment (MCI) and metabolize of oxygen-derived free radicals, acetylcholinesterase(AchE), blood lipids and inflammatory mediator. Methods Memory,the levels of superoxide dismutase (SOD), malondialdehyde (MDA), AchE, blood lipids, IL-1? and IL-6 of MIC group(45 cases) and elder healthy group(45 controls) were measured, funther compare and correlative analysis were made.Results Compared with elder healthy group, memory score in each item and memory quotient in MCI group were significantly lower (allP