OBJECTIVE:To provide reference for clinical safe administration and drug instruction revision through analyzing 552 cases of ADR/ADE induced by Sodium aescinate for injection. METHODS:Using“aescinate”and“ADR”as search words,re-trieved from CBM,CNKI,VIP and Wanfang database,47 literatures were included according to inclusion and exclusion criteria. The data was extracted and analyzed statistically. The drug instructions of twelve domestic pharmaceutical enterprises were analyzed statistically. RESULTS:The ADR/ADEs may involve multiple organs and systems,the most common reactions were venous irritant reactions as phlebitis,venous injury,etc. The drug instruction lacked some parts of information. CONCLUSIONS:Great impor-tance should be attaches to ADR/ADEs caused by Sodium aescinate for injection,and improvement of the drug instruction to en-sure the safety of drug use in the clinic.

Objective To study the significance of circadian gene Period2 expression in epithelial ovarian cancer tissues and the effect of gene overexpression on the growth of ovarian cancer xenografts in nude mice. Methods Twenty-two cases of ovarian cancer paraffin specimens in the First Hospital of Shanxi Medical University (ovarian cancer group) were chosed during Jau. 2010 to Dec. 2013, including 8 cases of stageⅠ, 8 cases of stageⅡ, and 6 cases of stageⅢ, while 6 cases of benign ovarian epithelial tumor paraffin specimens were selected as control (benign tumor group). Period2 gene were detected by real-time quantitative PCR and western blot methods in different stages of ovarian cancer tumor tissues. Established theovarian cancer xenografts in nude mice with ovarian cancer cell line SKOV3, and they weredivided into 3 groups (n=8), including the recombinant plasmid group, empty plasmid group and control group. Using gene transfection technique to transfer Period2 gene into tumor tissues, tested the expression of Period2 mRNA in tumor tissues by real-time quantitative PCR after transfection into all nude mice, monitoredthetransplant tumor growth and calculating the tumor inhibition rate,detected the antiapoptotic gene BRE, apoptosis related tumor necrosis factor receptor (TNFR1) andtumor suppressor gene NIX in tumor tissues by real-time PCR and western blot in different groups. Results (1)The expression level of Period2 mRNA in tumor tissues amongovarian cancer group stageⅠ,ⅡandⅢwere respectively 2.59±0.50, 0.47± 0.08 and 0.42 ± 0.08, but benign tumor group was 6.59 ± 1.05. The expression level of Period2 protein in ovarian cancer group stage Ⅰ,Ⅱ and Ⅲ were respectively 0.835 ± 0.087, 0.412 ± 0.035 and 0.199 ± 0.031, while benign tumor group was 0.874 ± 0.094. The expression level of Period2 mRNA and protein in benign tumor group was higher than those in ovarian cancer group stageⅠ,ⅡorⅢ(P<0.01). With ovarian cancer stage increased, the expression of Period2 mRNA and protein were decreased or absent (P<0.05).(2)Two weeks after transfection, the expression level of Period2 mRNA in recombinant plasmid group tumor tissue was significantly higher than those in the empty plasmid group or the control group (6.11±0.56 vs 0.50±0.09 vs 0.44 ± 0.08, respectively;P<0.01), the transplanted tumor volume of recombinant plasmid group was significantly less than those in empty plasmid group or the control group[ (486±70) mm3 vs (835±106) mm3 vs (846 ± 110) mm3, respectively;P<0.01], the tumor inhibition rate of the recombinantin plasmid group was as high as 42.9%, that was significantly higher than those in the empty plasmid group and the control group (3.8% and 0, respectively;P<0.05).(3)The expression level of BRE mRNA and protein in transplanted tumor tissues in the recombinant plasmid group were significantly lower than those in empty plasmid group and the control group;the expression level of TNFR1 and NIX were significantly higher than those in the empty plasmid group and the control group (all P<0.05). Conclusions Period2 mRNA and protein expression are absent in ovarian cancer of advanced stage. Transfection and stable expression of Period2 gene could slow down the growth of ovarian cancer, and the tumor inhibition rate could be significantly increased. Period2 gene may promote ovarian cancer cells apoptosis through inhibition of BRE gene expression and promoting TNFR1, NIX gene expressionto exert anti-tumor effect.

AIM: To investigate the expression of aquaporins-1 (AQP-1)in cultured bovine corneal endothelial cells and to explore the role of AQP-1 in corneal endothelial fluid transport.METHODS:The bovine corneal cells were cultured in DMEM containing 200mL/L neonate bovine serum. AQP-1 expression in the bovine corneal endothelial cells was detected with immunohistochemistry method before and after treatment of the cells with aquaporin inhibitor, p-chloromercuribenzene sulfonate. The osmotic water permeability was determined bymonitoring volume changes of cultured bovine corneal endothelial cells.RESULTS: AQP-1 expression in the membrane of cultured bovine corneal endothelial cells was revealed by positive staining (in brown color). The reading of osmotic water permeability of the cultured bovine corneal endothelial cells before treatment with p-chloromercuribenzene sulfonate was 0.044±0.005cm/s, which significantly decreased to 0.017 0.003 cm/s(n= 15) after treatment.CONCLUSION: AQP-1 expressed in the membrane of cultured bovine corneal endothelial cells may play an important role in fluid transport of corneal endothelial cells. Alteration of the AQP-1 expression may cause abnormal corneal function and corneal edema.

OBJECTIVE:To explore the general rules and characteristics of ADR/ADE induced by Glycerol fructose injection and provide reference for clinical ration drug use. METHODS:284 cases of ADR/ADE induced by Glycerol fructose injection from Jan. 2004 to Oct. 2014 in Drug ADR Monitoring Center of Henan Province were statistically analyzed and comparatively analyzed with the drug instructions in 11 domestic drug manufacturing enterprises. RESULTS:ADR/ADE induced by Glycerol fructose injec-tion was related to gender,mainly adult male,and ADR/ADE might occurred within 30 min;main clinical manifestations were skin and its appendages damages,medication site damage and systemic damage. There were larger differences among the notes de-scription in the package inserts from 11 drug manufact urers. CONCLUSIONS:The clinic should strengthen the monitoring of ADR/ADE induced by Glycerol fructose injection,try to avoid or reduce the incidence of ADR,improve the drug instructions and ensure medication safety.

[Objective]To summarize the professor WU Lianzhong's experience in treatment of spasmodic torticollis and myelitis sequela by using acupuncture,so as to guide clinical practice better.[Methods] From following professor WU Lianzhong clinical studies,studying professor WU Lianzhong's medical cases,introducing Professor WU to treat spasmodic torticollis with Du channel four points and five points and treat myelitis sequela by using acupuncture and traditional Chinese medicine,we can summarize his academic thoughts and experience on treating spasmodic torticollis and myelitis sequela.[Results] Professor WU thinks the key pathogenesis of Du channel type spastic torticollis is Orifices blocked and consciousness delirium,so the treatment principle is to clear the heart and reduce the fire,and regulate consciousness,at the same time strengthen the Du channel and break the nodules,this method is effective in clinical treatment.He holds that the etiology and pathogenesis of myelitis mainly for lack of spirit,attack of exogenous pathogens,prolonged illness lead to deficiency of liver and kidney,qi and blood deficiency,all these lead to tendon and vessel malnutrition.The cardinal treatment principle is to nourish liver and kidney,tonify qi and blood,dredge the channels and collaterals,also comprehensivey treat this disease by acupuncture combined with traditional Chinese medicine,the clinical effect is satisfying.[Conclusion] Professor WU's experience in treatment of Du channel type spasmodic torticollis and myelitis sequela is worth studying and popularizing in clinic.

Objective: To study the effect of EEG biofeedback in the treatment of children with ADHD and comorbid tic disorder. Methods:14 children with ADHD and comorbid tic (criteria of DSM-IV) aged 7 to 14 received EEG biofeedback treatment (average 34 sessions). The outcome was evaluated with Conners Parent Symptom Questionnaire(PSQ),Rutter Questionnaire,Achenbach CBCL,and C-WISC-R,WMS,CPT before and after treatment. YGTSS was used to evaluate the severity of tic disorder. Results: Hyperactivity index in PSQ, the total score of Rutter, and hyperactivity factor in CBCL were greatly reduced after treatment (P

Hepatitis B virus infection can result in different clinical outcomes which consist of self-limited infection(virus clearance),asymtomatic carrier status,chronic hepatitis B and cirrhosis.The mechanism led to individual discrepancy for outcome of hepatitis B virus infection is unknown yet.It is suspected that the outcomes of hepatitis B virus infection might be associated with the inherent factors of host.The results of association studies between genetic polymorphisms of HLA-I,Ⅱ class,TNF-? and VDR and different clinical outcome of hepatitis B virus infection in recent years are reviewed.

Internet not only substantially improve the efficiency of work, but also realize the sharing of information resources to the broadest extend. At present, China has the world's largest number of Internet users and the large basis network, a very serious problem is that Internet security issues. The issue of the status on network security of domestic and abroad is discussed and the related recommendations of internet security are putted forward.

Birds,especially wild waterfowls,are a natural reservoir of influenza virus,though poultry,mammalians and even human beings are also thought to be the potential hosts.Influenza virus can be classified into subtypes according to the characteristics of the nucleocapsid protein and membrane protein,each subtype with its own most adaptable host,so called host range restriction.However,the outbreak and spreading of influenza in different species has aroused increasing attention since its disastrous consequence on the ecological system of the earth.Large-scale epidemic survey,prediction of possible mutation of the virus,development and application of vaccines are key actions to be taken to prevent the pandemic of influenza.This article offers an overview of the ecology of influenza virus,features of recent great outbreaks of avian influenza,and relevant issues of concern.