With advances on drug genomics of leukemia,the drug toxicity has been found to be associated with the drug metabolism gene polymorphisms in children with acute lymphoblastic leukemia.Current researches are to focuse on how to find a genotype to predict methotrexate toxicity effectively and treat patients individually.This review illustrates the association between three drug-metabolizing enzyme gene polymorphisms including methylenetetrahydrofolate reductase,reduced folate carrer and glutathione S-transferase and toxicity of methotrexate.

Objective To explore the clinical value of Holter heart rate turbulence in diagnosis of patients at high risk for coronary heart disease.Methods 110 patients with suspected coronary artery disease,according to the results of coronary angiography (CAG),were divided into CAG positive group (67 cases) and CAG negative group (43 cases).Heart rate turbulence and heart rate variability were analyzed in two groups.The atrial fibrillation,atrial flutter,and pseudo-differential interference were removed,the qualified ventricular contraction was selected.Two parameters of HRT,the initial shock value (TO) and turbulence slope value (TS) were calculated.Results The average value of TO and TS was (0.36 ± 0.15) ％,(1.98 ± 0.52) ms/RR in positive group,those in the negative group were (-0.87 ± 0.36) ％ and (4.83 ± 0.65) ms/RR,there were statistically significant differences between two groups (t =1.953,1.976,all P ＜ 0.05).After 1 year follow-up,9 cases (13.44％) died in positive group,2 cases (4.65％) died in negative group,the difference was statistically significant (x2 =5.476,P ＜ 0.05).Conclusion The heart rate turbulence with dynamic cardiogram can help to early diagnose the patients at high risk of coronary heart disease.It was important to improve the survival rate of patients.The method is simple,inexpensive,effective,which is worth promoting in the clinic.

Objective To explore the efficacy and safety of hormone replacement therapy with combination of Fosamax and simple use of Fosamax in osteoporosis and steopenia of perimenopausal women.Methods One hundred and thirteen perimenopausal women with osteoporosis accepted treatment in Outpatient Clinic of Panyu Convalescent Hospital of Guangzhou from December 2010 to June 2014 were chosen and divided into the control group (n=55) and the observation group (n=58) according to therapeutic method.The control group was treated with Fosamax alone while the observation group was treated with HRT basing on the simple use of Fosamax.The therapeutic effect,lumbar and hip bone mineral density(BMD) level,tibial ultrasound velocity (SOS) and T values of tibia,gonadal hormone concentrations,endometrial thickness and adverse reaction of the two groups before and after the therapy were compared.Results (1) The total effective rate of the observation group after the treatment was 93.10％,significantly higher than tbat of the control group (78.18％,x2=3.829,P=0.034).(2) Before and after treatment in two groups,there were statistically significant differences in terms of lumbar spine BMD,hip BMD,SOS of tibia,blood follicle stimulating prime (FSH) and estradiol(E2) (P＜0.05),and there were statistically significant differences between the two groups after the treatment in terms of lumbar spine BMD,hip B MD,SOS of tibia,FSH and E2 (t =3.389,5.012,3.997,5.829,3.902;P=0.035,0.028,0.033,0.024,0.038).(3) There was statistically significant differences in term of the incidence of adverse reactions between the observation group and the control group (6.90％ vs.16.36％;x2=8.273,P=0.012).Conclusion Compared with alone use of Fosamax,the therapeutic effect of hormone replacement therapy with combination of Fosamax and in osteoporosis and steopenia of perimenopausal women is more significant with low occurrence rate of adverse reaction.It is worth popularization.

The research to improve outcomes in critically ill patients through nutrition support has steadily progressed over the past 4 decades. One current approach to this problem is the addition of specific nutrients as primary therapy to improve host defenses and improve the outcomes of critically ill patients. The field is referred to as“pharmaconutrition”,focusing investigations on each nutrient to understand its pharma-cological effects on immune and clinical outcomes. The purpose of this review was to introduce some of the known pharmaconutrients such as glutamine,arginine,ω-3 fatty acids,vitamin C,zinc,and selenium,regard-ing critical ill adults and children.

Cytokine-effects of soluble CD23(sCD23) were investigated in our laboratory. We found that sCD23 prepared by ourself could increase the migration inhibition effect of nomal peripheral blood leukocytes stimulated with PHA. It is proposed that sCD23 may have cytokine-like bioactivities on activated T cell and regulate immune response via an unknown mechanism.

Objective To analyze the laser lacryocystoplasty combined with antimetabolites in the treatment of lacrimal duct obstruction clinical curative effect. Methods 72 cases of lacrimal duct from July 2015 to February 2017 in our hospital for treatment of COPD patients as the research object, 84 eyes (23 eyes with nasolacrimal duct obstruction and chronic dacryocystitis 16 eyes in tears tubular obstruction and 45 eyes). All patients underwent laser angioplasty combined with tired to anti metabolic drug treatment, postoperative flushing of lacrimal duct, and for a period of 3 months of follow-up of patients, analysis of clinical treatment in this group of patients with complications of surgery. Results After 3 months of treatment , 71 eyes were cured, accounting for 84.52％; 10 eyes were better, accounting for 11.90％; 3 eyes of treatment failure, accounting for 3.57％. patients in this group had 1 cases of orbital hematoma in nerve block anesthesia, symptomatic treatment returned to normal 1 weeks after surgery, the complication rate was 1.19％. Conclusion The application of laser lacryocystoplasty combined with anti drug metabolism of lacrimal duct obstruction were treated with simple operation, obvious curative effect and low complication rate advantages, has the value of clinical application, but should strictly control the application of Dose Mitomycin, postoperative flushing of lacrimal duct, and good antibacterial prophylaxis, and to reduce the The incidence of the disease and the effect of clinical treatment.

Objective:Through research of corneal transparency and related influential factors,the authors try to look for some clinical variety regulations after penetrating keratoplasty(PKP) in order to enhance the rate of surgery success.Methods:104 blind patients due to corneal disease (107 eyes) undergone PKP were followed up on months 1,3,6,12,18,24 and 36.Clarity of cornea,corneal endothelial cells and all kinds of complications were recorded besides regular examinations.Results:Grafts were transparent in 82 of 107 eyes on month 1(76.6%),in 69 of 90 eyes on months 6 (76.7%),in 51 of 81 eyes on months 12(63%),in 26 of 40 eyes on months 12 (65%) after PKP.Corrected vision were improved especially in patients of grafts transparency.Vision of patients with transparent grafts were more than 0.1 in 67 of 82 patients(82%) on month 1,in 59 of 63 patients(94%) on months 3,in 66 of 69 patients(96%) on months 6.Conclusion:PKP is an important method to corneal disease blind patients.Rejection was the major cause affecting graft transparency.

Objective:To analyze and determine the main component in volatilization oil which is extracted from the Chinese herbal medicine Kaempferia galanga L.Methods:Volatilization oil is obtained by steam-distillation from commercial available Kaempferia galanga L..After re-crystallization,GC-MS,UV-Vis,NMR,IR were processed for analysis and structure determination.Results:The m/z=205 peak in MS represents the quasi-molecular ion peak.The strongest absorption peak at 304nm in UV-Vis shows that there is big conjugated system in the molecule.Along with the data of chemical shifts and coupling constants of 1H-NMR,13C-NMR and wave numbers of IR absorption peaks,the main ingredient of Kaempferia galanga L.volatilization oil is verified as ethyl 3-(4-methoxyphenyl)-(E)-acrylate.Conclusion:We have employed advanced methods in separation and analysis in the determination of structure of the main ingredient in volatilization oil extracted from Kaempferia galanga L.and get detailed data to support the results,the methods are reliable and the results are dependable.

Based on the data of the ISI "Journal Citation Report" in 2006 edition,a statistics for the 88 mi-crobiology science journals has been made. The results showed that these 88 learned journals were published in 16 countries with 3 languages,especially English. And the main countries were United States,England,Netherlands and Japan. The average data of articles and total cites of the 88 journals were 165 and 5226.64,respectively. Moreover,the average data of impact factor and immediacy factor were 3.154,0.45 and 5.84,respectively. Finally,fifty-five learned journals with impact factor over 2.0 were introduced from these jour-nals to be referenced.

Objective:To investigate the effect of brain tissue extract of hypoxia-preconditioned mice (BHP) on tolerance of NGF-induced differentiated PC12 cells to hypoxia and the optimal concentration for protective effect.Methods:The mice model of acute repetitive hypoxia was reproduced and BHP was prepared.BHP was added into the cultures of 4～5 day differentiated PC12 cells and the final protein concentrations of BHP were 1.0、10.0、100.0?g/ml(BHP groups) respectively.Brain tissue ex- tract of normal mice(BN) at the same three protein concentrations were used as controls(BN groups) and PBS solvent control groups were also prepared in which only PBS was added(PBS groups).Then differentiated PC12 cells were cultured in hypoxia (2%O_2).After 24,48,72 hours'hypoxia,colorimetric method(OD_(570nm)) of tetrazolium salt MTT staining was adopted to determine cell viability and lactate dehydrogenase(LDH) leakage assays were also conducted at the same time.Besides,apoptotic per- centages at late stage(72 hours' hypoxia) were detected.Results:After 24 hours' hypoxia,OD_(570nm) values of BHP groups with protein concentrations being 10.0、100.0?g/ml were significantly higher,but their LDH leakage percentages were significantly lower than those of BN groups with the same protein concentrations and of hypoxic PBS group.With hypoxia prolonging,BHP gradually lost the protective effect.At the time point of 72 hours' hypoxia,OD_(570nm) values of only BHP group at 100.0?g/ml protein concentration was significantly higher than that of the corresponding BN groups,its LDH leakage percentage and apop- totic percentage was significantly lower than those of the hypoxic PBS group.Conclusion:The effects of BHP on tolerance of differentiated PC12 cells to hypoxia depend on its concentrations and on its treating time.Besides,BHP at final concentration of 100.01?g/ml protein showed steady,prolonged protective effect.