1.The development of recombinant factor Ⅷ concentrates for clinical application
International Journal of Pediatrics 2011;38(6):548-550
Replacement therapy with plasma derived or recombinant F Ⅷ (rFⅧ) concentrates is only effective way to treat hemophilia A.Currently,the first,second and third generation rFⅧ are commercially available.Of the three products,the third generation rFⅧ products are best,because of no additional human or animal plasma proteins at any period.The use of rFⅧ concentrates has greatly improved the safety of replacement therapy in hemophilia A and reduced the risk of blood-borne pathogen transmission.In this article we review the current knowledge about the three rF Ⅷ concentrates,analyzing their main characteristic and their clinical hemostatic efficacy and safety.
2.An Empirical Study of EMP Innovation in 7-year-program Clinical Medicine of Southeast University
Chinese Journal of Medical Education Research 2002;0(01):-
A series of innovations in EMP teaching have been carried out in the 7-year-program clinical medicine from such aspects as curriculum design,teaching methodology and strategies,form of examination and establishment of an open teaching system.This paper is to compare the 5 language skills before and after the innovation.
3.Changes of Cerebral Haemodynamics in Neonates with Hypoxic-Ischemic Encephalopathy Treated with Shengmai Injection
li, JIANG ; li, HUANG ; hong-guang, SUN
Journal of Applied Clinical Pediatrics 2006;0(18):-
Objective To study the changes of cerebral haemodynamics in neonates with hypoxic-ischemic encephalopathy(HIE) treated with Shengmai injection compared with citicoline,and explore the clinical interventions of Shengmai injection on HIE.Methods Fifty patients with HIE were randomized into 2 groups: shengmai group treated with Shengmai injection(n=30),and citicoline group treated with citicoline(n=20).The haemodynamic parameters included peak systolic flow velocity(PSFV),end-diastolic flow velocity(EDFV),pulsation index(PI) and resistance index(RI) of anterior and middle cerebral artery were detected by color Doppler ultrasound before treatment and on 7~(th) day after treatment respectively.Control group(n=50) compared with the two groups.Results PSFV,PDFV and RI of HIE patients were significantly lower than those of control group(P
4.An optimal method for cryopreservation of microamount round spermatids of the mouse.
Hong JIANG ; Li WANG ; Cun-li WANG
National Journal of Andrology 2015;21(8):698-701
OBJECTIVETo search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.
METHODSWe compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).
RESULTSUnder the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).
CONCLUSIONA high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.
Animals ; Cell Survival ; Cryopreservation ; methods ; Cryoprotective Agents ; administration & dosage ; pharmacology ; Glycerol ; administration & dosage ; pharmacology ; Male ; Mice ; Spermatids ; Time Factors ; Vitrification ; drug effects
5.Relationship among insulin-like growth factor 1、insulin-like growth factor binding protein 3 and human fetal development
xian-ping, HONG ; li-hong, JIANG ; ge-li, LIU
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To study the relationship among insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein 3(IGFBP-3) and birth weight, length and placental weight and to discuss the endocrine factors which affected fetal growth by assaying levels of IGF-1、IGFBP-3 in umbilical cord serum.Methods The neonates were divided into appropriate for gestational age infant(AGA) and small for gestational age infant(SGA) according to birth weight and gestation. The birth weight, length and placental weight of neonates were measured and at meantime the levels of IGF-1 and IGFBP-3 were assayed by immunoradiometric assay.Results 1.There were 105 neonates, including 79 AGA and 26 SGA. There were significantly statistical differences in birth weight, length and placental weight (all, P
6.Effects of bone marrow mononuclear cell implantation induced by 5-azacytidine on cardiac function
Hongxin XU ; Gengshan LI ; Hong JIANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(02):-
Objective: Recent studies have shown that cell implantation can replace infarcted myocardium to improve cardiac performance. The present study was designed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation into myocardium bordering the infarction with or without induced by 5-azacytidine on cardiac function after acute myocardial infarction (AMI) in rabbits. Methods: AMI was replicated by ligating the left anterior descending coronary artery. Rabbits were randomly divided into three groups: (1) BM -MNC induced by 5-azacytidine implantation (n=7), (2) BM-MNC implantation alo ne (n=7), and (3) AMI control (n=7). In addition, sham-operated (n=5) rabbit s were randomly selected to serve as non-infarction control. Animals for cell im p lantation were received intramyocardial injection of autologous BM-MNC in myoca rdium bordering the infarction, and echocardiography and hemodynamic studies wer e performed to evaluate cardiac function following 28 days of implantation. Results: Compared with the sham-operated group, the left ventricle (LV) end diastolic pressure (LVEDP) was significantly increased (P0.05). Conclusion: BM-MNC induced by 5-azacytidine implantation into myocardium bordering the infarction can significantly improve impaired cardiac function associated with LV remodeling after AMI, however such improvement is not further promoted compared with that in BM-MNC implantation group alone.
7.Changes of apoptosis and expression of related apoptosis regulatory factors in neointima after canine coronary angioplasty
Xiaomin NIE ; Gengshan LI ; Hong JIANG
Chinese Journal of Interventional Cardiology 1993;0(03):-
Objective To investigate the changes of apoptosis and expression of related apoptosis regulatory factors in neointima after canine coronary angioplasty Methods Stents were implanted in the left circumflex coronary artery in 15 canines Arteries were harvested at 1, 4, and 12 weeks after stenting Uninjured arteries were used as controls Apoptosis was demonstrated by the terminal uridine nick end labeling (TUNEL) method and transmission electron microscopy Proliferating cells were identified by immunostaining for proliferating cell nuclear antigen (PCNA) Expressions of Bcl xl proteins were detected by immunohistochemical method and Western blot Results Stents implantment induced intimal hyperplasia Apoptosis was not detected in control vessels Apoptotic cells and PCNA positive cells were identified at 1, 4, and 12 weeks with a peak at 1 week Profiles of apoptosis and cell proliferation after stenting were accordant in neointima, but cell proliferation rates were higher than cell apoptosis rates at all time points Expressions of Bcl-xl proteins were detected at 1 week, peaked at 4 weeks, and lasted till 12 weeks after stenting Conclusion Apoptosis may be an important determinant of in stent restenosis Bcl xl appears to play a critical role in regulating cell apoptosis
8.Nutrition support of gastrointestinal tumor patient
Zheng HONG ; Zhiwei JIANG ; Jieshou LI ;
Parenteral & Enteral Nutrition 1997;0(04):-
Gastrointestinal tumor patients are characterized by malnutrition resulting from various elements. So it is necessary to analyze,assess, and treat malnutrition of patients with gastrointestinal tumor. Nutrition support is an indispensable step in tumor therapy.
9.Relationship between prognosis and complications of patients with severe viral hepatitis
Li JIANG ; Hong LEI ; Xuqing ZHANG ;
Journal of Third Military Medical University 2002;0(12):-
Objective To investigate the relation between the prognosis and complication of patients with severe viral hepatitis (SVH) Methods The incidences of different complications in 573 patients with SVH were analyzed The survival rates of SVH patients with different complications were compared Results Complications were diagnosed in 77 8% SVH patients The incidences of hepatic encephalopathy, infection, brain edema, gastrointestinal hemorrhage, and hepatorenal syndrome were 50 4%, 34 6%, 30 2%, 22 2%, and 21 8%, respectively The survival rate of SVH patients without complications was 78 7%, significantly higher than that of patients with complications (13 7%, P
10.Study on the mechanism of S100A9-induced secretion of vascular endothelial growth factor-A by monocytes
Chunlong LI ; Hong LIAO ; Ming JIANG
International Journal of Laboratory Medicine 2015;(13):1816-1817,1820
Objective To explore the molecular mechanism of S100A9-induced secretion of vascular endothelial growth factor-A (VEGF-A)by monocytes.Methods Peripheral blood specimen were collected from healthy individuals undergoing physical exami-nation and the CD14 + monocytes were purified by using immunomagnetic beads and the expression of the receptor for advanced gly-cation endproducts (RAGE)was detected by flow cytomertry.In vitro CD14 + monocytes were stimulated by S100A9,and anti-RAGE antibody or NK-κB signal pathway inhibitor were pre-incubated for 1 hour and then stimulated by S100A9,the levels of VEGF-A were detected by using enzyme-linked immunosorbent assay.Results The high level of RAGE was expressed by isolated CD14 + monocytes,after S100A9 stimulation,the secretion of VEGF-A by CD14 + monocytes was significantly increased in a dose and time dependent manner.However,the inducing VEGF-A was significantly decreased(P <0.01 ),while pre-treated with anti-RAGE antibody or NK-κB inhibitor (P <0.01).Conclusion S100A9 inducing the secretion of VEGF-A by monocytes and is de-pended on RAGE-NK-κB signal pathway,suggesting that S100A9 might promote angiogenesis.