[Objective]To evaluate the reliability of proximal femoral nail anti-rotation(PFNA) in treatment of intertrochanteric fracture using K-wire apex distance(KAD) intraoperatively to control tip apex distance(TAD).[Method]From January 2007 to January 2009,44 cases of intertrochanteric fractures were treated by closed reduction internal fixation with PFNA.Intra-operative KAD and post-operative TAD were measured and analyzed for their correlation.[Result]Follow-up was given to 42 patients for 6-18 months(averaged,10 months).Bone union was achieved after 11-23 weeks(averaged,13 weeks).Intra-operative KAD were 13-31 mm,with an average of 21.75 mm.Post-operative TAD were 18-35 mm,with an average of 24.61 mm.Recurrence of cerebral infarction was found in 1 case.No infection,deep vein thrombosis,intramedullary nailing rupture,screw broken or femoral fracture was found.[Conclusion]With the help of intra-operative fluoroscopy,the distance from the end of Kirschner's wire to joint surface(KAD) plays an important role in controlling TAD,and it could prevent relative complications such as helical blade cutting-out of femoral head.

Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy

Objective To investigate the changes in the metabolism of neurotransmitters in different regions of the brain induced by propofol in healthy volunteers using the proton magnetic resonance spectroscopy (MRS) technology.Methods IH-MRS was performed in ten 20-40 year old healthy volunteers. Each volunteer underwent MRS scan twice. The first MRS scan was performed when they were conscious as baseline control value. The second scan was performed during target-controlled infusion (TCI) of propofol. The target effect-site concentration was set at 3.0 ?g?ml-1. Volume of interest (VOI) included sensory cortex, motor cortex, thalamus, hippocampus and basal ganglia. The metabolites in the spectra included N-acetyl-aspartic acid (NAA), glutamic acid (Glu), GABA, choline compounds (Cho) and creatine (Cr) .Results During TCI of propofol MAP and RR were significantly decreased ( P 0.05) as compared to the baseline value when the volunteers were conscious. During TCI of propofol the NAA content in thalamus and hippocampus, Glu content in thalamus, hippocampus and basal ganglia and Cho content in all the 5 regions of the brain were significantly decreased ( P

Objective To develop a time saving and sensitive cell culture system based on hepatitis C virus chimera expressing enhanced green fluorescent protein(EGFP) and to facilitate the study on HCV pathogenesis and screening of anti-HCV drugs.Methods Enhanced green fluorescent protein reporter gene and a mutation V2440L that can yield higher virus titers were introduced into the C-terminus of non-structural protein 5A (NS5A) of the JFH1 viral genome by using recombinant PCR.The viral RNA was transfected into Huh7.5 cells.Viral RNA in supernatant of HCV RNA-transfected cells was determined after transfection by RT-PCR.HCV replication and infection were determined by immunofluorescence assay.IFN-α was used to evaluate the feasibility of this system for anti-HCV drugs screening.Results The viral RNA replicated efficiently in transfected cells.These cells can produce HCV-EGFP reporter virus.Viral RNA levels in supernatant were 3.06× 105 copies/ml and 7.96×106 copies/ml at 72 h and 9 d after transfection,respectively.The virus titer reached to 104 FFU/ml 9 d after transfection.The expression of EGFP was inhibited by IFN-α in a dose dependent manner in Huh7.5 cells infected by HCV-EGFP reporter virus.Conclusion The recombinant HCV JFH1-EGFP reporter gene system is a time saving,cost effective and sensitive method for studying viral replication cycles and screening of anti-HCV drugs.

ObjectiveTo develop a cell culture system with consistent expression of whole hepatitis C virus (HCV) gene and Renilla luciferase gene and to facilitate the study on HCV pathogenesis and the screening of new antiviral drugs.MethodsRenilla luciferase (RLuc) reporter gene and a mutation that could yield higher virus gene expression were introduced into the C-terminus of non-structural protein 5A (NS5A) of the JFH1 viral genome by using recombinant PCR.The viral RNA was transfected into Huh7.5 cells.Naǐve Huh7.5 cells were infected by the supernatant from the viral RNA transfected cells.HCV replication and infection were determined by virus titration,Renilla luciferase assay,immunofluorescence assay and western blotting.IFN-α was used to evaluate the feasibility of this system for anti-HCV new drug screening.ResultsThe viral RNA replicated efficiently in transfected cells.These cells could produce high titer of HCV-Rluc reporter virus and the virus titer reached to 1.5 × 104 FFU/ml at day 15 of posttransfection.The activity of Renilla luciferase was inhibited by IFN-α in a dose dependent manner in Huh7.5 cells infected by HCV-Rluc reporter virus.ConclusionThe recombinant HCV-JFH1-Rluc reporter gene system is sensitive and efficient.It can be a useful tool for high throughput screening of anti-HCV drugs.

AIM: To observe the humoral immune response in adult rhesus monkey induced by A? 1-15 vaccine. METHODS: 5 adult male rhesus monkeys were injected intramuscularly with A? 1-15 vac cine at baseline and at week 2, 6, 10, 14, 18, 22. The titer and IgG isotypes of the antibody against A? 1-42 in the serum were measured with ELISA. The specificity of the antibody against A? 1-42 was determined by Wester n blotting. The A? plaques in Tg2576 transgenic mouse brain were stained with t he antisera using immunohistochemistry method. RESULTS: At the eighth week after the vaccination, antibody against A? 1-42 bega n to develop significantly i n the serum. The titers of the antibody increased following vaccine boosted and reached 1: 3 840 at the twenty-fourth week, then decreased after the terminat ion o f inocunation. The IgG1 was accounted for the highest level in the antisera pool . The antibody against A? 1-42 showed high specificity. The A? plaques in Tg2576 transgenic mouse brain were labeled with the antisera. CONCLUSION: A? 1-15 vacci ne could induce vigorously specific humoral immune responses in adult rhesus mon key.

Objective:To develop a convenient,economical and stable model of acute lung inflammation in mice.Methods:BALB/c mice were inhaled intranasally with 50 ?L of LPS(1 g/L) or sterile PBS,and sacrificed at different time points after being anaesthetized.The bronchoalveolar lavage fluid(BALF) was collected,and the lungs were separated and homogenated or embedded and sliced to 5 ?m sections,which were then stained by HE to determine the severity of inflammation.The inflammatory cell infiltration in bronchoalveolar lavage was counted and IL-1?,the pro-inflammatory cytokine,measured by ELISA in lung homogenate and BALF.Results:The data showed that administration with 50 ?g of LPS(1 g/L) for 2 h resulted in significant inflammation in the lung.LPS mainly stimulated the recruitment of neutrophils within 24 h.And LPS was a quick revulsant of IL-1? production in BALF and in lung tissue between 4 and 24 h.Macrophages and lymphocytes recruited after 1 day,and sustained for at least 3 days.Conclusion:The results indicate that intranasal administration of LPS can induce a rapid and stable acute inflammatory model in mice.

Dermatitis, Occupational ; diagnosis ; Diagnostic Errors ; Female ; Humans ; Trichloroethylene ; adverse effects ; Young Adult

Objective To investigate the value of high-frequency ultrasound in diagnosis of thyroid carcinoma by comparative analysis of high-frequency ultrasound and spiral CT imaging results. Methods The imaging results of patients with thyroid carcinoma proved by postoperative pathology or biopsy results were reviewed.High-frequency ultrasound and spiral CT were used to examine the 35 patients in The Tumor Hospital of Harbin Medical University between 2007 and 2009. Results Diagnosis of thyroid carcinoma by application of Highfrequency ultrasound were 27 cases, diagnosis rate was 77.1%(27/35);by spiral CT were 25 cases, diagnosis rate was 71.4% (25/35);comparison of the two methods showed no significant difference (x2= 0.3, P ＞ 0.05). Combined application of high-frequency ultrasound and spiral CT diagnosed 33 patients with thyroid carcinoma, diagnosis rate was 94.3%(33/35), which was significantly higher than that of high-frequency ultrasound alone or that of spiral CT alone(compared with high-frequency ultrasound, x2 = 4.2, P ＜ 0.05;and spiral CT, x2 = 6.4, P ＜ 0.05). Conclusion Combined application of high-frequency ultrasound and spiral CT can improve the diagnosis rate of thyroid carcinoma.

Petroleum-field emulsions,which are produced in the process of exploration,production and re-covery,represent a major environmental and processing problem for the petroleum industry.Biological de-mulsifier as a biological agent,will become a novel method of petroleum-field emulsions demulsifier instead of chemical demulsifier for its high efficiency,low cost,high adoptability,environment-friendly,repeated use.A set of high efficiency and convenient method was established to isolate de-emulsification strain and studied on the fermentation conditions of the de-emulsification strains.A de-emulsification strains,isolated from a petroleum-contaminated site,was evaluated for its de-emulsification capabilities using a kero-sene/water model emulsion system keeping stable for more than 200 hours which was mixed by stirring Tween 60-water(0.072%,V/V)and Span 60-oil(0.028%,V/V)at the ratio(oil:water)of 6.5:3.5.We have isolated two strains with high and steady de-emulsification activity by separation and purification,blood-plate test,excluding-oil test and de-emulsifying test.The average de-emulsification rates were more than 80.0% at 24 h.The two strains are both belonging to bacillus by traditional and molecular genetic iden-tification.By optimizing the demulsification bacteria fermentation conditions,the fermentation conditions,which is the most suitable for accumulating the fermentation products,were determined as follows:tem-perature 25?C,stirring speed160 r/min,pH 9,inoculated quantities 20%.When the fermentation conditions were temperature 35?C,stirring speed120 r/min,pH 9,inoculated quantities 2%,the fermentation products appeared the highest activities of demulsification.