Objective To explore the effect of Chitosan gel (Ⅲ) on the complications from Milligan-Morgan hemorrhoidectomy for treatment of severe mixed hemorrhoids.Methods Seventy-six patients with severe mixed hemorrhoids were randomly divided into treatment group and control group with 38 cases in each group. All patients from the two groups were treated with Milligan-Morgan hemorrhoidectomy. Then Chitosan gel (Ⅲ) was used in the treatment group. The two groups were compared in terms of curative effect, pain level, complications and hospital stay.Results There was no significant difference in the curative effect (P>0.05). But the scores on pain level, hemtochezia, infection and edema of the treatment group were all significantly lower than those of the control group (allP<0.05) and the hospital stay was significantly shorter than that of the control group (P<0.05).Conclusions In the treatment of severe mixed hemorrhoids by Milligan-Morgan hemorrhoidectomy, Chitosan gel (Ⅲ) is effective in relieving pains, reducing hemorrhage, edema and preventing infections and therefore shortens the hospital stay. In view of nursing, it is critical to perform mental care as well as close observations on complications.

Fibrosis is the major cause of corneal scarring.Transforming growth factor-beta (TGF-β) plays a key role in corneal homeostasis and repair.Corneal epithelial basement membrane is thought to be the important barrier of corneal epitheliumstroma interaction.In different stages of corneal wound healing,the isoforms of TGF-β have different temporal and spatial expression.The integrity of basement membrane is a critical factor of these procedures.The temporal and spatial distributions of TGF-β isoforms play the crucial roles in cell migration,proliferation,phenotype changes and deposition of extracellular matrix in corneal wound healing.It is the mechanism of corneal scarring and scar-free healing.This article reviews recent articles to elucidate the biological functions of TGF-β and the temporal and spatial distribution of its isoforms in corneal wound healing.

Early diagnosis of prediabetic peripheral neuropathy depends on the evaluation of small fibers.Traditional nerve conduction tests can only evaluate the function of large myelinated fibers,while lack of sensitivity to small fibrous lesions.These lesions were related to pain and autonomic neuropathy.In recent years,with the progress of neurophysiological diagnosis technology,the early diagnosis of diabetic peripheral neuropathy has been improved.Clinical methods,commonly used in the detection of small fibrous lesions,mainly include skin sympathetic response,quantitative sensory test,contact heat pain evoked potential,and quantitative sudomotor axonal reflex test.In this paper,the pathogenesis of diabetic peripheral neuropathy,neurological pathological changes and applications of electrophysiology technology were reviewed to provide an objective basis for early diagnosis of prediabetic peripheral neuropathy.

BACKGROUND:How does the peripheral nerve block work to inhibit the functions? What effects does it do to the spinal cord and the cerebral center? How does it regulate the peripheral nerve and how does it change anatomy of brain center? Al above are stil unknown.
OBJECTIVE:To observe the effect of peripheral nerve block on the biology of spinal cord nerve cel s.
METHODS: Sixty New Zealand rabbits were selected and randomly divided into three groups: ischiadic nerve block group, subarachnoid block group and extradural nerve block group. Each group had 20 rabbits which were sub-divided into experimental group and control group with 10 rabbits in each subgroup. The rabbits in the three experimental groups were injected with lidocaine, bupivacaine and lidocaine at the middle point between femoral head and ischiadic tuberosity in ischiadic nerve block group, subarachnoid block group and extradural nerve block group, respectively. The rabbits in each control group were injected with normal saline at the same position.
RESULTS AND CONCLUSION:After ischiadic nerve block, extradural nerve block or subarachnoid block for 30 minutes, the Nigeria’s bodies of the little round cel s in the poliomyelia posterior horn laminaeⅢandⅣand the polyhedral cel s in the anterior horn laminaeⅨwere less than those in the control groups. Nuclei leaned towards one side. c-Fos protein expression was decreased or showed no expression, suggesting that the cord nerve cel function of corresponding spinal segments was inhibited. The spinal pia mater of spinal cord had a hierarchical or fracture phenomenon after subarachnoid block.

0.05). The maj or toxicity included Neutropenia, nausea and vomiting. The severity of these sid e effects was mild to moderate and well tolerated. No severe toxicity was observ ed. Conclusions:Gemcitabine plus etoposide (GV) and Gencitabine plus cisplatin were both active regimen for advanced NSCLC, with high efficecy but talerated toxicity.

Angiogenesis plays an important role in tumor growth and metastasis,anti-angiogenic therapy is becoming the center point in tumor therapy,endostatin is a recently discovered endogenous inhibitor of angiogenesis.Endostatin can specifically target endothelial cells,inhibiting proliferation and migration and inducting apoptosis.This review describes the mechanism of endostatin in inhibiting angiogenesis,induction of apoptosis in tumor cells and clinical trials.

Objective To compare the clinical effects between hysteroscopic electroresection and open hysterectomy in the treatment of intrauterine benign diseases. Methods A total of 58 women with intrauterine benign received hysteroscopic electroresection (hysteroscopic group) and 60 women underwent open total hysterectomy (control group). The operating time, hospital stay, postoperative recovery and cure rates were compared between the two groups, respectively. Results The operating time was 68.4?20.6 min in control group and 55.2?19.7 min in hysteroscopic group ( t=-3.555, P =0.000). The postoperative hospital stay was 7.2?1.8 d and 4.5?1.5 d in control and hysteroscopic group, respectively ( t=-8.836, P =0.000). The time to resume regular work was 64.4?25.3 d in control group and 37.2?7.8 d in hysteroscopic group ( t=-7.835, P =0.000). The cure rates in control and hysteroscopic group were 100% (60/60) and 94.7% (54/57), respectively ( ? 2 =1.477, P =0.224). Conclusions Hysteroscopic electroresection gives good short-term therapeutic effects and quick postoperative recovery. It may replace hysterectomy in part of patients with intrauterine benign diseases.

Objective To established a method for the content determination of ginsenoside Rg1 in Yushangling Capsules by HPLC. Methods HPLC was used with the C18 column. The mobile phase was acetonitrile-0.5% phosphoric acid (24∶76). The flow rate was 1.0 mL/min, the column temperature was 25 ℃, and the detection wave was set at 205 nm. Results The calibration curve was linear in the range of 0.05～0.80 ?g. The regression equation was Y =3629375.5X+2517.1, r =0.9998. The average recovery was 100.48% and RSD was 1.59%. Conclusion The method is simple, accurate and suitable for the content determination of ginsenoside Rg1 in Yushangling Capsules.

Objective To investigte the possibility of interventional mechanical thrombectomy dealing with chronic vascular thrombus, and assessing its curative effects. Methods Twenty three cases included 17 males 6 females, and aged from 46 to 80 year with a mean of 64.8. All patients were coinsided with MRA, vascular ultrasound and angiography, in which 12 patients with chronic heart and vascular disease, and 11 patients with serious diabetes. Thrombi were located in iliac artery ( n=9,L=4,R=3 , bilaterals=2), femoral artery( n =2), populiteus artery ( n =2), popliteus artery( n =3), iliac vein ( n =7, L=5,R=2), portal vein ( n =1). The length of thrombi varied from 3 to 8 cm, and the diameter from 5 to 10mm.Filter should be placed in inferior cava vein before venous thrombus treatment ( n =7). Mechanical thrombectomy was undertaken as following: ATD ( n=9, Vein=7), PTD( n =2), and Oasis( n =1). After guide wire passing through thrombus segment, Oasis was inserted for remove thrombus. Urokinase (500 000U) was usually administered for catheter directed thombolysis before/during mechanical thrombectomy. Stent should be placed in the segment if stenosis was confirmed after thrombectomy angiography. Drugs were used after thrombectomy including thromboyltic drug, heparinzed anticoagulation and changing microcirculation. Vascular ultrasound, reconstruction CTA and clinical follow up had all been carried out. Results 12 cases (85.7) were undergone successfully thrombectomy. One case failured with portal vein thrombus formation, the other with multiple segments involvement in femoral artery was treated by combined ATD and Oasis. Sy mptoms of 15 cases were either released or disappared, including ischemia, swelling and motion limitations. The patency shown by vascular ultrasound follow up were 100% in three months, 85.4% in six months, 73.2% in twelve months, with simultaneously blood flow improvement to normal and obviously corrected 76.5%,65.4% 60.1% in 3,6,12 month respectively.Conclusions Interventional mechanical thrombectomy is a new choice of treating chronic vascular thrombus with its direct curative effects, but still needs long term follow up.

Objective To investigate the effect of heme oxygenase-1 ( HO-1) on brain edema in a rat model of asphyxial cardiac arrest and resuscitation. Methods Forty male SD rats weighing 250-300 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation; group II cardiac arrest (CA); group Ⅰ Hemin (HO-1 inducer) and group IV SnPP (HO-1 inhibitor). Asphyxial cardiac arrest and resuscitation were performed in CA, Hemin and SnPP groups (group Ⅰ ,Ⅲ, Ⅳ) . Hemin and SnPP groups received hemin 15 mg/kg intraperitoneally (IP) at 12 h before CA and SnPP K 30 μmol/kg IP at 1 h before CA respectively. The animals were sacrificed at 1 and 6 h after recovery of spontaneous circulation (ROSC) . The water content of the cortex, hippocampus and brain stem and the expression of HO-1 and aquaporin-4 (AQP4) mRNA in cortex and hippocampus ( by RT-PCR) were determined. Results Water content of cortex and hippocampus was significantly higher at 1 h after ROSC in CA and SnPP groups than in sham operation group and was significantly lower in Hemin group than in CA group. There was no significant difference in water content of brain stem at 1 and 6 h after ROSC among all 4 groups. The expression of AQP4 mRNA was significantly higher in cortex and hippocampus at 1 h after ROSC in CA and SnPP groups than in sham operation group and was significantly lower in Hemin group than in CA group.Conclusion HO-1 can reduce brain water content at early stage after cardiac arrest and resuscitation by regulating the expression of AQP4.