Abnormal expression of microRNA (miRNA) and aberration epigenetic regulation can both contribute to cancer development. miRNA expression can be changed by epigenetic regulation such as DNA methylation and histone acetylation, thereby regulates relative oncogenes and tumor-suppressor genes, and consequently leads to carcinogenesis.

This study aims to investigate the effect of γ-Secretase Inhibitor DAPT, (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), on the differentiation of neural precursor cells and the production of neurons in the neural precursor cell line GE6. GE6 was cultured in medium with 4 μmol/L DAPT added as the experimental group and the untreated medium separately as the control group. After 4 days of differentiation, we carried out the following experiments. We used immuno-fluorescent staining to observe the ratio of Tuj1, GFAP and O4 positive cells. We also used qRT-PCR to detect the effect of the DAPT on Tuj1 and GFAP mRNA transcription in the GE6. The results of immuno-fluorescent staining indicated that the Tuj1 ratio of experimental group was higher compared to that of the control group, but the GFAP and O4 ratio of experimental group was lower than that of the control group. The differences were statistically significant (P < 0.05). The result of qRT-PCR was in accordance with immunofluorescent staining results. It was well concluded that DAPT could promote the neurogenic differentiation of neural precursor cell line rather than leading to gliogenic differentiation. More neurons could be obtained for transplantation with the addition of DAPT.
Amyloid Precursor Protein Secretases ; antagonists & inhibitors ; Animals ; Cell Differentiation ; drug effects ; Cell Line ; Dipeptides ; pharmacology ; Neural Stem Cells ; drug effects ; Neurons ; Rats

Helicobacter pylori infection in children can cause a variety of gastrointestinal and extra-digestive diseases, even the gastric cancer.Most Helicobacter pylori infections are acquired in childhood and can cause various manifestations such as nausea, vomiting, anorexia, idiopathic thrombocytopenic purpura, refractory iron deficiency anemia, and growth retardation.In recent years, many advances have been made in the study of children′s Helicobacter pylori infection.This review focuses on the risk factors of Helicobacter pylori infection in children, containing the perspectives of Helicobacter pylori strains, hosts and environment, and looks forward to further research directions.

OBJECTIVE To estimate the value of Mycoplasma pneumoniae(Mp)-IgM,C-reactive protein(CRP) and WBC on diagnosis of children respiratory infection.METHODS Serum Mp-IgM antibody,CRP and WBC were tested in 225 children with respiratory infection by passive particle agglutination,ITM immunoturbidimetry and analyzer of blood cell.RESULTS Forty-six cases of 225 children were identified positive in Mp-IgM.The positive rate of Mp-IgM was 20.4%.Compared the CRP and WBC among cases positive in Mp-IgM,bacterial infected and virus infected,there was significant difference bewteen cases positive in Mp-IgM and bacterial infected,no significant difference bewteen cases positive in Mp-IgM and virus infected.CONCLUSIONS The joint detection of serum Mp-IgM antibody,CRP and WBC can be a valuable clue to diagnosis of children respiratory infection.

Objective To compare the therapeutic effects of recurrent pterygium treated by microsur- gical management.Methods Sixty-two cases(67 eyes)with recurrent pterygiumwere randomly divided into limbal stem cell autograft transplantation comblined with mitomycin C(34 cases38 eyesand limbal stem cell autograft transplantation(28 cases29 eyes)The post operative follow-up period was 6 to 30 months.Re- suits One eye recurrence was noted in the trial groupthe recurrent rate was 2.63%Three eyes recurrence was noted in the control groupthe recurrent rate was 10.34%There was statistical significant difference be- tween two groups(P0.05).Conclusion Limbal stem cells autograft transplantation combined with mitomycin C can decrease the recurrent rate.It is an ideal methods of recurrent pterygium surgical procedureis worth spreading.

Head and Neck Neoplasms ; surgery ; Humans ; Neck ; surgery ; Neck Dissection ; methods ; Thyroid Diseases ; surgery ; Vascular Surgical Procedures ; methods

Objective To study the preventive effects of selective bile ductal cannulation using an aqua-coat tipped Metro tracer guidewire on pancreatitis and hyperamylasemia after ERCP. Methods A total of 291 patients with biliary disease were enrolled in the study. The patients were randomized into the guidewire group (n= 149) and the control group (n= 142). In the guidewire group, the bile duct was first accessed by insertion of an aqua-coat tipped Metro tracer (diameter 0. 029 inch) guidewire through a 5.5Fdouble channel ERCP-tube to judge the bile duct by the direction of guidewire. As soon as cannulation was accomplished, the guidewire was removed to allow injection of contrast medium and other therapeutic manipulation. In the control group, the bile duct was opacified by the traditional method of cannulation of the biliary tree followed by single ERCP-tube cannulation, judgment of the bile duct by injection of contrast medium and performance of other therapeutic procedures. The incidences of post-ERCP pancreatitis and hyperamylasemia were compared between the 2 groups. Results The selective bile ductal cannulation was successfully conducted in 145 patients in the guidewire group and 138 in the control group. The incidences of post-ERCP pancreatitis and hyperamylasemia were significantly lower in the guidewire group than in the control group. Pancreatitis tended to be less severe for patients in the guidewire group. Conclusion Using the Double-Crossing method in selective bile ductal cannulation with an aqua-coat tipped Metro tracer guidewire reduced not only the frequencies of post-ERCP pancreatitis and hyperamylasemia but also the severity of post-ERCP pancreatitis.

Objective To understand the air microorganisms pollution and the community structure and distribution characteristics of outdoor airborne microbes in Hefei. Methods Airborne microbes were sampled by fallen plate method at 10 collection sites in the Hefei city. Results The results showed that the average concentration of the bacteria,fungi and total microbes in the outdoor environment were 5.04?104,3.75?103 and 5.41?104 cfu/m3 respectively and the fungi accounted for 6.92%of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%,36.0%,4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes. The distribution of the airborne microbes has the characteristics of time and space.It is affected not only by the environment and pollution,but also by such factors as human activities,animals and plants.

With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.
Autolysis ; Beer ; Echinocandins ; genetics ; Glucosyltransferases ; genetics ; Hypergravity ; Membrane Proteins ; genetics ; Saccharomyces cerevisiae ; cytology ; Saccharomyces cerevisiae Proteins ; genetics

AIM:To evaluate changes in tear film stability and meibomian gland function and the clinic efficacy of three different artificial tears in patients with preoperative dry eye after phacoemulsification.METHODS:All 90 cataract patients (90 eyes) with preoperative dry eye who underwent phacoemulsification randomly divided into three groups (Group A treated with protein-free calf blood extract ophthalmic gel;Group B treated with sodium hyaluronate eye drops;Group C treated with Vitamin A palmitate eye gel).Ocular Surface Disease Index (OSDI), Schimer's I test(SⅠt), tear film break time (BUT), corneal fluorescein staining (FL) and meibography were performed for all patients preoperatively and 7, 30 and 90d postoperatively.RESULTS:No statistical differences existed among the three preoperative groups (P>0.05).Except meibomian gland score, there was no statistical significance among preoperatively and 7, 30, 90d postoperatively of the three groups (P>0.05).At 7d postoperatively, SⅠt and BUT of every group were lower than those before treatment, FL scores and OSDI scores were higher than those preoperative (P<0.05);there were no statistical differences among the three groups(P>0.05).At 30d postoperatively, SIt, BUT, OSDI scores in group A and C were better than in group B, which displayed statistical differences (P<0.05);FL scores in group A were significantly better than in group B and C (P<0.05).At 30, 90d postoperatively, SIt, BUT, FL scores, OSDI scores were better than preoperative results, which displayed statistical differences (P<0.05).There were no statistical differences among the three groups at 90d postoperatively (P>0.05).CONCLUSION:Tear film stability and meibomian gland function were affected by phacoemulsification.Topical application of deproteinised calf blood extract eye gel, sodium hyaluronate eye drops and Vitamin A palmitate eye gel all hase a clearly beneficial effect on subjective symptoms.Deproteinised calf blood extract eye gel and Vitamin A Palmitate Eye Gel had more powerful effect on BUT than sodium hyaluronate, however deproteinised calf blood extract eye gel is more effective in superficial punctuate keratopathy.