objective To study the relationships between TNF-β gene type in idiopathic dilated cardiomyopathy (IDC). Methods Eighty-six IDC patients were chosen as IDC group and 95 cases unrelated healthy people as control group in the First Hospital of Jilin University in 2008. The levels of TNF-β protein were detected with Enzyme-linked immunosorbent assay(ELISA ). TNF-β polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphisms(PSR-RFLP). Results The levels of TNF-β protein in IDC patients[(1.876±1.013) μ/L] were significantly higher than controls[(1.018±0.645)μg/L], the difference being statistically significant (t=2.674,P<0.01). The frequency of TNFβ*2 genotypes in IDC patients[63.4%(109/172] was significantly higher than controls[47.9%(91/190)], the difference being statistically significant(OR=1.88, X2=6.78, P<0.05). Conclusions The TNFβ*2 allele might related to idiopathic dilated cardiomyopathy (IDC), can also be considered as one of susceptible genes of IDC

Aim To investigate the effects of atorvas-tatin ( ATV) on activation and injury of microvascular endothelial cells induced by oxidized low density lipo-protein ( ox-LDL) . Methods Cultured human micro-vascular endothelial cells were pre-incubated with ATV for 24 h prior to exposure of endothelial cells to ox-LDL. After exposure of endothelial cells to ox-LDL, the cell viability was measured by MTT method, LDH in supernatants was determined by enzyme activity as-say kit, ICAM-1 in supernatants was assayed by using ELISA method, phosphorylation of NF-κB p65 was de-tected by western blot analysis, transcriptional activity of NF-κB signal pathway was measured by employing dual-luciferase reporter assay system. Results Hu-man microvascular endothelial cells were activated and injured by ox-LDL. Inhibition of the cell viability, re-lease of LDH, expression of ICAM-1, phosphorylation of NF-κB p65 , and up-regulated transcriptional activity of NF-κB induced by ox-LDL were attenuated by ATV. Conclusion ATV can significantly inhibit the activa-tion and injury of human microvascular endothelial cells induced by ox-LDL, and that may be related to inhibition of phosphorylation and transcriptional activity of NF-κB.

A novel fibrinogenolytic protease,named atrase A,has been purified from the venom of Naja atra by sequential chromatography.Atrase A is a single chain glycoprotein with a molecular weight of 64.6 kD,an isoelectric point of pH 9.6 and a neutral sugar content of 4.16%.Atrase A specifically and slowly degraded α-chain of fibrinogen.This fibrinogenolytic activity Was inhibited by chelating agents(EDTA,EGTA and 1,10-phenanthroline)and DTY,and partially inhibited by PMSF,but not by soybean trypsin inhibitor,indicating it is a metalloproteinase.Atrase A showed edema-inducing activity and bactericidal activity against Staphylococcusa aureus.Atrase A did not show cytotoxicity on A549 and K562 cells in MTT assay,but detached adherent A549 cells from the substrate.Atrase A did not show significant inhibition of platelet aggregation induced by ADP or collagen,and did not exhibit proteolytic activities towards fibrin,azocasein and BAEE.It also did not show hemorrhage activity when injected subcutaneously into mice.

Objective To evaluate the effect of enteral nutrition support on the nutritional status and clinical outcomes of patiems with dysphagia after stroke.Methods 148 patients with acute stroke and associated dysphagia were enrolled from August 2013 to July 2014 in the Fifth Affiliated Hospital of Zhengzhou University,and randomly divided into 2 groups by drawing lots within 48 hours after admission:enteral nutrition group (n =75,early enteral nutrition support) and control group (n =73,routine diet).The nutritional status (triceps skinfold thickness,levels of serum total protein,albumin and hemoglobin),incidence of lung infection,mortality,and neurological function were compared between the two groups on day 1 and day 21 after admission.Results On day 1 after admission,the triceps skinfold thickness,levels of serum total protein,albumin,and hemoglobin in the enteral nutrition group were not significantly different from those in the control group [(15.4 ±4.1) mm vs.(15.1 ± 3.7) mm,t=1.36,P=0.392; (75.7±2.6) g/Lvs.(76.6±3.1) g/L,t=1.12,P=0.254; (39.2± 1.8) g/Lvs.(38.7±2.1) g/L,t=1.24,P=0.200; (137.4±14.5) g/Lvs.(135.1±15.3) g/L,t=1.01,P =0.461].On day 21 after admission,all the 4 nutrition indicators were significantly higher in the enteral nutrition group than in the control group [(13.5 ±3.9) mmvs.(11.2±4.6) mm,t=2.08,P=0.019; (63.3±4.1) g/Lvs.(57.1±4.7) g/L,t=4.01,P=0.001; (35.7±1.6) g/Lvs.(34.1± 2.0) g/L,t=2.31,P=0.022; (125.7 ±17.9) g/Lvs.(120.3 ±16.7) g/L,t=2.39,P=0.027].The enteral nutrition group showed lower incidence of lung infection,mortality,and reduced scores of American National Institutes of Health Stroke Scale compared with the control group (41.3％ vs.63.2％,x2 =9.69,P =0.002; 15.3％ vs.21.2％,x2=3.27,P=0.014; 11.1 ±4.1 vs.14.7 ±3.9,t=2.98,P=0.007).Conclusions Early enteral nutrition support can improve the nutritional status and outcomes of stroke patients with dysphagia.In addition,early enteral nutrition support may also be helpful for the improvement of the neurological function.

Objective To develop an image analyzing procedure for automatic localization of facial features on infrared images.Methods An unsupervised local and global features extraction method was adopted for the localization of facial features of frontal view face image.First,a threshold was used to segment the image into foreground and background,and the nose was localized by considering the symmetry of the face.Second,Harris operator was adopted to detect interest points in a rectangular area covering all the facial features,and then local maximum of the interest points were detected.And finally,K-means clustering method was used to cluster the points and obtain the facial features localization.Results The experimental result of 100 images demonstrated that the procedure could automatically localize eyes,nose,mouth,and distinguish the feature areas.Conclusion The proposed infrared image analyzing procedure based on Harris operator and K-means clustering can be used to locate facial features on infrared image more rapidly and reliablely.

OBJECTIVE To study the epidemiology of drug-resistant and antiseptic-resistant genes of(productive)(enzyme) in meticillin-resistant coagulase negative Staphylococcus(MRCNS).METHODS mecA Gene of the(drug-)resistant ?-lactam,aac(6′)/aph(2″) and aph(3′)-Ⅲ genes of aminoglycoside-modifying enzyme(AME) and(ermA/B/C) genes of erythromycin methyltransferase were detected by polymerase chain reaction(PCR) in 40 MRCNS strains.RESULTS From them there were 39 strains with mecA gene,32 strains with aac(6′)/aph(2″) gene,15 strains with aph(3′) -Ⅲ gene,30 strains with ermA/B/C gene,2 strains with tetM and 6 strains with qacA/B gene.There were 26 strains(65.0%) simultaneously with mecA,aac(6′)/aph(2″)and(or) aph(3′)-Ⅲ and ermA/B/C genes in MRCNS.CONCLUSIONS The drug-resistant rate is higher in MRCNS.There are more than half tested strains simultaneously with 3 to 4 drug-resistant genes.

OBJECTIVE To find out the prevalent distribution and multidrug resistance trend of meticillin-resistant Staphylococcus(MRS) and to prevent fulminant prevalence of MRS and opt for effective therapeutic means.METHODS The bacterium was(identified) by the way of API Staph and the TH-16S′s coding tube.The(antimicrobial) susceptibility testing was adoped by ATB-STAPH5 and MRS was examined by dilution and K-B.All statistical analyses were performed using SPLM 3.0 software.RESULTS The isolation rate of meticillin-resistant S.aureus(MRSA) and meticillin-resistant coagulase negative Staphylococcus(MRCNS) was 38.09%,20.00%and 87.65%,89.00%,(respectively) in 2 years.Along with the age of patients,the infection from MRS was(increasing).The isolation rate of MRSA was 28-26%,but that of MRCNS was more than 80% from S.epidermidis,S.haemolyticus,S.hominis,and S.saprophyticus subsp saprophyticus.All parts of our body can be(infected) by MRS.The more than 30% MRSA were multidrug resistant and the approximately 11.87-13.75% MRCNS were also multidrug(resistant).(CONCLUSIONS) The isolation rate of MRSA from national surveillance(network) is not(different) with that of MRCNS.

Objective To analyze the training and evaluation standards on urine analyzers in the grass-roots medical unit.Methods The applicability,requirements,subject setup and items of the standards as well as the requirements of evaluation scoring chart were expounded in detail,and the related elementary knowledge was analyzed including the structure,working principle,installation and adjustment,operation,application,maintenance and etc of the analyzer.Combined personnel with the equipment was suggested to ensure the accuracy and high efficiency of the test result.Results The interpretation of the standards contributed to enhancing the quality of medical equipment training and the ability of practical support,and facilitated the examiner to perform annual evaluation with the scoring chart.Conclusion It is necessary to grasp accurately the connotation of the standards,to improve the quality of medical equipment training and to enhance combat support capability for the grass-roots medical unit and examiner.

Objective To explore the causes of accidental instability of SYSMEX XS-800i blood cell analyzer for WBC testing and their countermeasures.Methods Totally 10 pieces of specimen with high deviation were analyzed retrospectively, and the changes of DIFF-Y values of WBC were observed. Sysmex detergent was used to clean the pipelines including 4DL, 4DS and EPK pipes. Fluid path electromagnetic valve was checked and then repaired or replaced. Results It's proved that the results at the first time were not reliable, and there was significant differences between the results at the first and second times for DIFF-Y value, with P<0.01. The failures were eliminated after the above countermeasures were carried out.Conclusion WBC testing pipelines and electromagnetic valve of fluid path have to be cleaned periodically to eliminate the influence on DIFF-Y value of WBC and false increase of WBC counting.

Aim To investigate the effect of cobra venom metalloproteinase atrase A on human endothelial cell.Methods The effects of atrase A on the growth of HMEC were measured by MTT,SRB and morphological observation methods,respectively.After exposure to atrase A,IL-8,ICAM-1,MCP-1 and E-selectin released by HMEC were detected.Caspase-8 and caspase-3/7 were detected by fluorescent luminescence method.After intravenous injected atrase A to rats,the concentrations of ICAM-1,IL-8 and TNF-? in serum were measured.Results The results showed that atrase A(400,40 mg?L-1) significantly inhibited the growth of HMEC in low cell density.The microscopic examination revealed that atrase A detached the adherent HMEC.After exposure to atrase A,IL-8,ICAM-1 and MCP-1 released by HMEC were increased.Atrase A induced HMEC to express caspase-3/7 and caspase-8.After the administration of atrase A to rats,the concentrations of ICAM-1,IL-8 and TNF-? in serum were increased.There was no difference between the low-dose group and control group in our experiments.Conclusion Cobra venom metalloproteinase atrase A inhibits the growth of HMEC,and induces HMEC releasing inflammation mediators and apoptosis.