The mental health state of the 256 fourth-year medical students of Kunming Medical College was preliminarily analyzed by requiring. The students fill in the inquiry sheets (questionare) in a question-and-answer way. The ages of these 136 male and 120 female students ranged from 19 to 23 years old, and the self-desiged 109 items of the investigation were induced into 8 aspects related to the mental health such as the outlook on life etc. The result showed that these are differences between the mental health state of the senior medical students and the traditional ideas, morality, manners of education etc. Some initial points of view were put toward.

Protracted bacterial bronchitis (PBB) is a common cause of children chronic cough,especially in those aged 1 ～ 6 years.It is defined by the presence of persistent wet cough for longer than 4 weeks,but lacks of symptoms or signs of other alternative diagnosis of chronic cough.It shows a normal or near-normal chest radiograph,a intense neutrophilia in bronchoalveolar lavage fluid(BALF),and a chronic purulent bronchitis under bronchochoscopy,and usually resolves after two-week course of appropriate oral antibiotic (usually amoxicillin-clavulanatc).So far,PBB hasn't been fully recognized by most pediatricians,and often been unrecognised or misdiagnosed as asthma.In this paper,pathogenesis,diagnosis and treatment are reviewed.

Glucosylceramide synthase(GCS) plays an important role in ceramide metabolism and can block apoptosis signal pathway which is mediated by ceramide. Recently, studies have demonstrated that overex-pression of GCS exists in various muhidrug resistant cancer cell lines and is related to drug resistant pheno-type. A possible connection between GCS and P-gp is also suggested. Moreover,the level of GCS can be down-regulated to increase tumor cells sensitivity by chemical inhibitor and RNA interference. Thus, inhibition of ac-tivity and expression of GCS is a new mechanism of reversing muhidrug resistance.

Objective To explore the protective effect of Ligustrazine on pulmonary fibrosis caused by hyperoxia in neonatal rats.Met-hods Eighty Sprague-Dawley(SD)clean neonatal rats,which were less than 12 hours old,were enrolled in this study.The neonatal rats were randomly divided into 4 groups:air-exposed control group(group A);air-exposed plus Ligustrazine-treated group(group B);hype-roxia-exposed control group(group C);hyperoxia-exposed plus Ligustrazine-treated group(group D).The neonatal rats of group B and group D were intraperitoneally injected with Ligustrazine[30 mg/(kg?d)] in solution of normal saline(NS),group A and group C were intraperitoneally given the same dose of NS.On 14th day,10 rats of each group were randomly chosen and then killed,the pulmonary pathological changes were observed by HE staining under light microscope.The area percentages of collagen fibers were measured by Masson staining and imaging analysis.The degree of pulmonary fibrosis was evaluated by the area percentages of collagen fibers and hydroxyproline content.The results were analyzed by SPSS 13.0 software.Results On the 14th day of the exposure,the histopathologic examination demonstrated broadened lung interstitium and reduced alveolar quantity and lung fibrosis were developed in group C;Ligustrazine treatment obviously alle-viated the changes of lung histomorphology.The newborn rats in group C had lower body weight compared that of with group A,B(Pa0.05).Conclusion Early use of Ligustrazine can decrease the severity of the pulmonary fibrosis of neonatal rats exposed to hyperoxia.

With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.
Bioengineering ; Industrial Microbiology ; Pichia

The genesis of malignant tumor is proved to be mainly caused by regulatory disturbance of cell cycle,in which p27 protein and cyclin D1 protein are major regulators.Cyclin D1 and p27 are positive andnegative regulator respeetively.Recently,redueed expression of p27 protein and overexpression of cyclinD1 pro-tein are found in various eaacers.It has been demonstrated that p27and cyelinD1 are closely associated with the genesis and development of many cancers.Cell cycle and its regulatory has become the focus in tumor research.

Objective To study the variable number tandem repeats (VNTR) polymorphism in intron 4 of the endothelial nitric-oxide synthase (eNOS) gene and its relationship with plasma NO in pregnancy induced hypertension (PIH). Method The VNTR polymorphism in intron 4 of the eNOS genes of PIH and healthy control pregnant women were detected by PCR. The plasma level of NO~(3-)/NO~(2-) was measured by spectrophotometer, which was used as the index of NO synthesis.Data were analyzed according to the different type of genotypes and allelic gene frequency. Results The PIH group showed significant higher frequency of VNTR 4a allele and 4a/4b+4a/4a genotype than those of the control (16.00% vs 7.50%,P

Eighty-six patients with community-acquired pneumonia who suffered from cerebrovascular sequelae were admitted to hospital from 2005 to 2007.Patients were randomized into ticarcillin sodium/clavulanate potassium group and cefuroxime Sodium group.Sputum culture was performed for each case before and after treatment.Gram-negative bacilli accounted for 74%of infection,and the most common pathogens were klebsiella pneumoniae(26%)and Escherichia coli(14%).The response rates in two groups were 93%and 72%,respectively(χ2=6.54,P＜0.05).Bacteria eradication rates in two groups were 90%and 62%,respectively(χ2=6.00,P＜0.05).Ticarcillin sodium and clavulanate potassium is an effective antibiotic for community-acquired pneumonia in elderly patients with cerebrovascular sequelae.

AsoneoftheCdc25phosphatasefamilymembers,Cdc25Cplaysanimportantroleinregu-lating mitosis of eukaryotic cells.In eukaryotic cells,CDK1-cell cycle protein B (CyclinB)compound mainly control the process of G2-M.The activity of Cdc25 C is the key in cell cycle into M phase.It activates CDK1-cyclinB complexes to promote cells from G2 to M phase .Improving Cdc25 C activity can promote the G2-M phase transition,and remove the G2-M phase retardation induced by ionizing radiation,preventing the damaged DNA from repaired into the phase of cell division,resulting in cell death caused by excessive cell proliferation, thus enhance radiosensitivity.

Objective To investigate the clinical effect and safety of compound glycyrrhizin combined with bozhi glycopeptide in treatment of erythema nodosum.Methods Sixty-four patients with erythema nodosum were divided into treatment group (36 cases) and control group (28 cases) by random digits table method.The patients in treatment group were given compound glycyrrhizin 120 mg,intravenous drip,once a day;and bozhi glycopeptide 4 ml,intravenous drip,once a day.The patients in control group were given compound glycyrrhizin 120 mg,intravenous drip,once a day.The clinical efficacy and recurrence were compared between 2 groups.Results The total effective rate in treatment group was significantly higher than that in control group [86.1％ (31/36) vs.57.1％ (16/28)],there was statistical difference (P ＜ 0.05).Follow-up for 3 months,the rate of recurrence in treatment group was significantly lower than that in control group [16.1％(5/31) vs.8/16],there was statistical difference (P＜ 0.05).Conclusion Compound glycyrrhizin combined with bozhi glycopeptide can effectively control the erythema nodosum,and has no significant adverse reactions,reduces the rate of recurrence,and it is worthy of promotion and application.