Aberrant microRNA(miRNA) expression has been found in most of human cancers.miRNA-related regulation pathways,including the interaction between miRNA and protein-coding gene and the interaction between miRNA and IncRNA,have been shown correlated with the initiation and progression of human cancers.miRNA might be a therapeutic target in the treatment of malignancies.

Laboratory animal science is a preceding subject,which contains particular theory system and characteristics.In order to meet the developmental need of higher medical education and train more medical talents with high quality,the traditional teaching model must be reformed.A series of teaching reforms have been performed and analyzed in this paper.

AIM:To establish a method of determining effective components in Qianjin Shenanning Pills(Flemingia Philippinensis Merr.et Rolfe,Radix et Rhizoma Ginseng rubra,Radix et Rhizowa Notoginseng,etc). METHODS: The contents of ginseng saponin Rg_1,Rb_1 and notoginseng saponin R_1 were determined by HPLC. RESULTS: The four effective components could be separated and purified by macroporous adsorptive resins of D_(101) and n-butanol,and their contents could be determined. CONCLUSION: The method is simple,feasible and reproducibility is good,it can be used as quality control in medicinal preparation.

Objective To study the cryptosporidiosis infection among young children in 29 kindergartens in the province, and to explore the best way to diagnose the infection. Methods Stool specimens of 1 204 children were collected, oocysts of Cryptosporidium were identified with auarmine O-modified staining, acid-fast staining, safranine T and methylene blue staining, and auarmine O-modeified acid-fast staining. Results The detection rate of Cryptosporidium in four stainings were 2.46%,1.50%,1.98% and 3.46% respectively, and the rate was significantly higher by auarmine O-modified acid-fast staining than other stainings (P

Objective To investigate the protective effect of propofol (Pro) in combination with ligustrazin (Lig) on the liver against ischemia-reperfusion (I/R) injury and the mechanisms involved.Methods Fifty male SD rats weighing 240-280 g were randomly divided into 5 groups of 10 animals each: group Ⅰ received sham operation (sham); group Ⅱ I/R; group Ⅲ Pro + I/R; group Ⅳ Lig + I/R and group Ⅴ Pro + Lag + I/R. The animals were anesthetized with intraperitoneal pentobarbital 20 mg?kg-1 . Liver ischemia was produced by clamping the hepatic hilum for 30 min, then the clamp was removed for 60 min reperfusion. In group Ⅲ propofol was infused at 20 mg?kg-1 ?h-1 starting from 20 min before ischemia until hepatic hilum was clamped. In group Ⅳ a bolus of ligustrazin 60 mg?kg-1 was given i.v. 20 min before ischemia. In group Ⅴ propofol was infused and a bolus of ligustrazin was given i.v. as in group Ⅲ and Ⅳ 20 min before hepatic ischemia. Blood samples were taken at the end of 60 min reperfusion for determination of serum activities of ALT, AST and LDH and meanwhile a liver specimen was obtained for determination of MDA content, SOD and XOD activities and for electron microscopic examination. Results Serum ALT and AST activities were significantly higher in group Ⅱ , Ⅲ , Ⅳ and Ⅴ than in group Ⅰ (sham) (P

Aged ; Anthracosis ; microbiology ; Bacterial Proteins ; genetics ; China ; DNA Mutational Analysis ; DNA, Bacterial ; genetics ; DNA-Directed RNA Polymerases ; Drug Resistance, Bacterial ; genetics ; Humans ; L Forms ; genetics ; Middle Aged ; Mining ; Mutation ; Mycobacterium tuberculosis ; genetics ; isolation & purification ; Tuberculosis ; microbiology

Objective To screen microRNA (miRNA) that inhibit expression of the metastasisrelated gene ezrin in ovarian cancer cells and explore their correlation to the invasion and metastasis of ovarian cancer. Methods The differential expression of ezrin in two paired high-metastatic and lowmetastatic cell lines were examined by real time reverse transcription (RT)-PCR and western blot. A functional screen with microarray was employed to identify miRNA that were differentially expressed between SKOV3 and SKOV3ip cell lines. Three programs, TARGETSCAN ( http://www. targetscan. org ),MICROCOSM ( http ://www. ebi. ac. uk/enright-srv/microcosm/htdocs/targets/v5/) and PICTAR (http://www. pictar. mdc-berlin. de), were employed to identify all miRNA, which may inhibit the expression of ezrin and were differentially expressed between SKOV3 and SKOV3ip cells. To test the repressive potential of these miRNA, synthetic mimetics were transfected individually into SKOV3ip cells and endogenous ezrin expression levels monitored by western blot and real-time RT-PCR. Results ( 1 ) The mRNA average level of ezrin were (81.74 ± 5.34) -fold higher expression level in SKOV3ip versus SKOV3 cells ( P ＜ 0. 01 ), while (2. 61 ±0. 14)-fold in HO-8910PM versus HO-8910 cells (P ＜0. 01 ). Elevated protein level of ezrin were observed in SKOV3ip cells compared with that in SKOV3 cells, and the same that in HO-8910PM cells compared with HO-8910 cells. Paired SKOV3 and SKOV3ip cells were employed to study the more significant difference in ezrin expression between them. (2) By a functional screen using miRNA microarray combined with bioinformatics analysis,the miR-183 and miR-22 were indentified as two candidate miRNA,which may have the potential regulatory role in ezrin expression. Real time RT-PCR assays revealed that miR-183 and miR-22 were, respectively, an average of (5.84 ± 0.66)-fold and(6.67 ± 0.67)-fold higher expression level in SKOV3ip versus SKOV3 cells (P ＜0. 01 ), which were in agreement with the microarray data. A subsequent validation by western blot and real time RT-PCR revealed that over-expression of miR-183 and miR-22 could both lead to an obvious decrease in ezrin protein level,while there were not signicant difference in the level of ezrin mRNA( P ＞0. 05 ). Conclusion Increased expression of miR-183 and miR-22 may both repress the protein level of ezrin,indicating that miR-183 and miR-22 may bear a potential role in inhibiting ovarian cancer metastasis in a ezrin-mediated way.

Aged ; China ; Drug Resistance, Bacterial ; Humans ; L Forms ; drug effects ; Middle Aged ; Mining ; Mutation ; Mycobacterium tuberculosis ; drug effects ; Silicotuberculosis ; microbiology

Background and purpose:Previous studies have revealed potential roles for Ezrin in a variety of cancers. Ezrin participates in several functions including cell adhesion, motility and cell survival, and there is increasing evidence that it regulates tumour progression. This study aimed to determine the differential expression of Ezrin between cervical cancer tissues and normal controls and to explore the association of Ezrin expression with clinical pathological characteristics and with the expression of PCNA, MMP-2, MMP-9 and HPV E6 protein. Methods:Retrospective study was performed among 30 cervical cancer tissue samples which were diagnosed in our hospital from Jan. 2002 to Dec. 2006. Ten normal cervixes from uterine myomas were employed as normal controls. Semi-quantitative immunohistochemisty analysis was performed to detect the association of Ezrin expression with clinical pathological characteristics, and with expression of PCNA, MMP-2 and MMP-9, HPV E6 protein in cervical cancer tissue and normal controls. Analyze the association of Ezrin expression with the clinical characteristics. Results:The expression of Ezrin was strong and the distribution was diffuse mainly in the cytoplasm and membrane in cervical cancer tissues, while the normal cervical tissues was weak and mainly in the membrane. However, there was no signiifcant association between the expression of Ezrin and the clinical characteristics including tumor stage, histology and grade. Ezrin expression was positively correlated with the expression of MMP-2, MMP-9 and HPV E6 protein in cervical cancer tissues, except PCNA. Conclusion:Ezrin was over-expressed in cervical cancer tissues and may be involved in the progress of invasion and metastasis in cervical cancer.

Objective To explore the effect of different frequencies interference of electoracupuncture on acute stress of C57BL/6 mice. Methods Thirty male C57BL/6 mice were randomly divided into model group,2Hz electroacupunture group and 100 Hz electroacupunture group.After interfering 7 days by electroacupuncture,all the mice were carried forced swimming tests and tail suspension test,and the immobility time in 5 minutes were taken down. Results The immobility time of forced swimming test and tail suspension test of 2 Hz eletroacupuncture group(32.00±14.73)s,(72.50±20.44)s was shorter than those of 100 Hz electoracupuncture group(145.60±34.62)s,(124.22±21.67)s( P <0.05); and the immobility time of 2 Hz eletroacupuncture group was shorter than that of model group(129.50±33.94)s,(142.23±20.05)s( P <0.05); but the immobility time of 100 Hz electroacupuncture group was no difference with that of model group( P >0.05). Conclusions The effect of 2 Hz eletroacupuncture could improve the behaviors of the acute stress of C57BL/6 mice and had the more effectiveness on anti-depression.