Objective To investigate the effects of dexamethasone on the airway development and vasculogenesis in cultured human fetal lung exposed to hyperoxia.Methods Human fetal lung at the pseudoglandular stage of development were cultured either in normoxia(21%O2,5%CO2)or hyperoxia(95%O2,5% CO2)for 72 hours.Dexamethasone was added into the culture medium at the concentration of 10-6 mol/L.Whereas ethanol at the same concentration was as control.Harvested tissue wss stained for pancytokeratin to I dentify epithelial cells.CD31 aS a specific antibody of endothelial cell.The effects of lung morphometry were analyzed using computer-assisted image analysis software to ealeuhte the thickness of airways,surface area occupied by airways and the distance of vessels to the nearest airway as well as the surface area of blood vessels.Results The lung architecture remained largely unchanged in normoxia for 72 hours culture,whereas hyperoxic culture of lungs resulted in significant dilation of airways and thinning of epithelium.The surface area occopied by airways increased significantly(P＜0.05).The blood vessels in interstium were more close to the airway epithelium in the hyperoxic lung(50 μm)as compared with normoxia group.Meanwhile,the proportion of the blood wessels in interstium of hyperoxia group(4.0%)Was higher than those of normoxia group (4.2%)and hyperoxia with dexamethasone(5.4%)(P＜0.05).Dexamethasone has the protective effect on lungs exposed tO hyperoxia.Conclusion Our resulta showed that hyperoxia resulted in the simple airways and the decreased bkxxl vessel density in interstitium of cultared human fetal lung.Dexamethasone has protective effect on fetal lungs exposed to hyperoxia and can promote lung mature.

Objective: To observe the clinical efficacy of heat-sensitive moxibustion plus medications on senile osteoporosis (SOP), and to explore the related mechanisms. Methods: A total of 70 elderly participants with osteoporosis were randomly divided into an observation group and control group, with 35 cases in each group. The control group was treated with conventional drugs, and the observation group was treated with heat-sensitive moxibustion on the basis of the conventional drugs. Both groups were treated for 3 months. Before and after treatment, assessed the visual analog scale (VAS) and Oswestry disability index (ODI) scores, determined the bone mineral density of the participants' lumbar spine (L2-L4) and left femoral neck, and detected the participants' serum bone morphogenetic protein-2 (BMP-2) and osteoprotegerin (OPG) levels. Results: After treatment, the VAS scores of both groups were lower than before treatment (both P<0.05), and the VAS score of the observation group was significantly lower than that of the control group (P<0.05). After treatment, the bone mineral density values of the lumbar spine and left femoral neck in both groups were significantly higher than before treatment (both P<0.05), and the bone mineral density values of the observation group were higher than those of the control group (P<0.05). After treatment, the ODI scores of the two groups were lower than those before treatment (both P<0.05), and the ODI score of the observation group was lower than that of the control group (P<0.05). After treatment, the serum BMP-2 and OPG levels in the observation group were significantly higher than those in the control group (both P<0.05). Conclusion: Heat-sensitive moxibustion plus medications for SOP can significantly relieve patients' pain, improve dysfunction, and increase bone density, which may be related to the improvement of the serum BMP-2 and OPG levels.

OBJECTIVE To study the level of plasma fibrinogen in patients with common facial paralysis, and investigate the relationship between the plasma fibrinogen and the pathogenesis of facial paralysis.METHODS A total of 157 inpatients with different kinds of facial paralysis were studied from December 2006 to December 2008.They were etiologically divided into five groups:severe Bell's palsy group(60 cases), moderate Bell's palsy group(19 cases), Ramsay-Hunt syndrome group(28 cases), temporal bone fracture with facial palsy(28 cases), and facial nerve tumor group(22 cases).They were tested for plasma fibrinogen level right after admitted to hospital.RESULTS Plasma fibrinogen level in severe Bell's palsy group was(4.08?0.90)g/L, which was significantly higher than that in other groups and the normal value(2.0～4.0)g/L(P

The use of optimal regulatory sequences for simultaneous expression of the transgenes might play a significant role in engineering plants with increased disease and insect resistance.The plant expression vector pOMS-GUS,which contained the GUS gene under the control of a chimeric promoter based upon the mannopine synthase(mas)promoter and the octopine synthase(ocs)enhancer,was constructed.Used as control,another vector pMAS-GUS,carried the GUS gene driven by only the mas promoter.The two vectors were introduced into tobacco plants by Agrobacterium-mediated transformation.Fluorometric assays for GUS activity and reverse transcription-polymerase chain reaction(RT-PCR)analysis revealed that GUS gene expressed weakly with untreated transgenic tobacco while the level of GUS activity increased steadily after 1 h subjected to wounding.The expression of the mas and ocs/mas promoters was induced a further 1.8-fold and 5.7-fold,respectively.SA(1 mmol/L)or MJ(250 ?mol/L)treatment also caused a large induction of the ocs/mas chimeric promoter;And the application of SA in combination with MJ(1 mmol/LSA & 250 ?mol/L MJ)produced an additive effect that exceeded the wounding response.The results showed that the ocs/mas chimeric promoter is a strong inducible promoter that can be activated by various stresses.The chimeric promoter should have utility in development of disease and insect resistant transgenic crops.

Objective To explore the possibility of human neutrophil peptide 1 (HNP1) gene engineering, we construct the eukaryotic expression vector carrying HNP1 gene. Methods With RNA extracted from human neutrophil cell as template, cDNA encoding mature HNP1 was amplified by RT-PCR, and then it was inserted into vector pMD18-T. After restriction endonuclease digestion and DNA sequencing confirmation the gene was subcloned into eukaryotic expression plasmid pcDNA3.1/V5-His-TOPO to construct a recombinant expression plasmid pcDNA3.1/V5-His-TOPO/HNP1, then the recombinant plasmid was transfected into COS-7 cells by lipofectamine, and the expressed product was identified by biotin-avidin enzyme-linked immunosorbent assay ( BA-ELISA). Results The sequence of HNP1 completely matched those published in GenBank, thus eukaryotic expression vector pcDNA3.1/V5-His-TOPO/HNP1 was constructed correctly. The ELISA results showed that the eukaryotic expression plasmid pcDNA3.1/V5-His-TOPO/HNP1 could temporarily express HNP1 in COS-7 cells. Conclusion The successful construction and expression of pcDNA3.1/V5-His-TOPO/HNP1 pave the way for the stable expression HNP1 in mammalian engineering cells.

Objective To localize precisely cerebral central sulcus on the coronal MR imaging. Methods The serieses of 4 mm thickness coronal sections and coronal MR images of brain were prepared on 30 cadaver heads. The preparation of the brain slices in correspondence with MR scan took the line perpendicular to the midpoint of intercommissural line as base one. The important cerebral gyri of cadaver were painted different colors. The coronal MR images of 6 volunteers were also prepared. The localization of central sulcus on the coronal sections was studied by the comparison of MR images with serial coronal sections of the brains. Results On the section of the central sulcus appearing for the first time, the appearing rate of dorsal thalamus was: left side 93%, right side 97%; the appearing rate of pyramidal tract was: left side 20%, right side 13%. The central sulcus almost disappeared on the section through the posterior border of splenium of corpus callosum, the appearing rate of the central sulcus on the superolateral surface was 33% on the both sides. Conclusion The central sulcus appears for the first time on the 1-2 sections which the dorsal thalamus appears on but the pyramidal tract doesn′t appear. On the section through pyramidal tract, the superior frontal, precentral and postcentral gyri can be identified according to the three medullary branches on superolateral surface of cerebral hemispheres. The central sulcus has almost disappeared on the section through the posterior border of splenium of corpus callosum.

Objective To understand the self-rated sub-health status among medical students and its main relative influencing factors. Methods By random stratified and cluster sampling, 878 medical students were selected to investigate sub-health status using Self-rated Health Measurement Scale-the Revised Version 1.0(SRHMS V1.0) and analyzed the related main influencing factors including gender, grade and specialty in college, daily study time, daily online time and cost of living per month. Results The results showed that the proportion of total good-health status among medical students was 21.2%. The prevalent rate of total sub-health status was 54.9%. The prevalent rates of physical, mental and social sub-health status were 49.3%, 28.6% and 39.2%, respectively; and theses rates in female students was significantly higher than those in male students. The prevalent rate of total sub-health status was significant difference with levels of daily online time (?2=15.544, P=0.001). The composition of total health status was significant difference with levels of grades, specialty and daily online time (P=0.000, P=0.005, P=0.000). The proportion of total bad-health and sub-health status was significant difference with levels of grades and specialty, and grade two students and clinical students took the highest proportion. Conclusions The prevalent rate of sub-health status among medical students was generally existent, and mental and physical health among medical students was mainly affected by gender, grade, specialty and daily online time. We should take proper intervention and adjustment to promote medical students' health.

Objective To understand the personality and self-rated health status and the relationship between them in medical students. Methods By multi-stage cluster random sampling, 878 medical students were selected to investigate the personality by Eysenck Personality Questionnaire-Revised (Short Scale For Chinese) and the health status by self-rated health measurement scale (SRHMS1.0). Results The score of L dimension among male and female medical students was significantly higher than that of nation- wide young population. The scores of E, N and P dimensions had no significant difference between them. The score of P dimension of male students was significantly higher than that of female students. The score of students' SRHMS was 339.28?41.19, which was higher than that of general population in China. The score of physical health subscale among male and female students was 144.90?11.41 and 142.73?11.36, respectively (t=2.829, P=0.005). The scores of SRHMS and its three subscales were significantly different in medical students with different personality traits except the score of physical health subscale in students with different grades of P dimension. The scores of E and L dimensions had significantly positive correlation with SRHMS, and the scores of N and P dimensions had significantly negative correlation with SRHMS. Conclusion The personality traits of medical students were remarkably influenced on their self-rated health. To encourage medical students to reform the wholesome personality will be helpful to their health.

OBJECTIVE:To study the preparation technic and determination of the content and dissolubility of the fluconazole-?-cyclodextrin inclusion complex(FCIC) METHODS:The FCIC was prepared by coprecipitation method and ultr_asonic method The content and percentage of the accumulated dissolubility of FCIC were determined by UV-spectrophotometry RESULTS:Fluconazole and ?-cyclodextrin could form inclusion complex through either preparation technology The contents of fluconazole in the complex were(11 34?0 77)% and(11 23?0 24)% respectively The dissolubility met the standard in CP CONCLUSION:Stable complex could be formed between fluconazole and ?-cyclodextrin

Objective To study the pathological characteristic of VX2 hepatic tumor in rabbit and the value of ultrasound in detecting this tumor mode. Methods To establish a model of Rabbit Liver VX2 Tumor with ultrasound- guided implantation of constitution mass and ultrasounds scanning was performed at fourteen days and twentyone days after implantation. After that, all the rabbits were executed and pathological examination was performed. Results Twenty models of Rabbit Liver VX2 Tumor were successfully established. The fourteen days after implantation,18 rabbits were found tumor,tumor diameter 8. 6 ～ 11.8 mm, average diameter ( 10. 15 ± 0. 67) mm; The twenty one days after implantation,20 rabbits were found tumor, tumor diameter 13.2 ～ 18.3 mm, average diameter( 16. 13 ±1.98)mm. Ultrasound showed that the tumor in the rabbit liver was a single spherical or sphere-alike hypoechoic nudes,and there were higher blood flow signals around the nedus. In pathological examination,the tumors were exserted to the liver surface,the cross sections of the tumor were gray,the cells of the tumors were bigger than the normal liver cells,arranged into nests with fibrous tissue. Conclusion In pathological examination, the heteromorphism of VX2 tumor cell was conspicuous. The growth of VX2 hepatoma could be monitored by ultrasound effectively.