Objective To investigate expressions of microRNA-21 (miR-21),PTEN,NF-κB,and caspase-9 in colon carcinoma and their possible mechanisms in progression of tumor.Methods Expressions of above-mentioned indexes and percentage of apoptotic cells in colon carcinoma,colon adenoma,and normal tissue specimen were detected.Results Expressions of miR-21,PTEN,N F-κB,and caspase9 in normal tissue were 0.39 ±0.02,50％,25％,and 50％.Expressions of them in colon adenoma were 0.62 ±0.06,50％,35％,and 55％,and those in colon carcinoma were 0.88 ±0.04,30％,75％,and 20％.Percentage of apoptotic cells were (24.64 ± 7.66) ％,(15.86 ± 1.44) ％,and (6.20 ± 2.57) ％,respectively.The above P-values were all less than 0.05.Expression of miR-21 was positively correlated with TNM stage,depth of infiltration,differentiated level and expression of NF-κB while inversely correlated with PTEN and caspase-9 expressions and percentage of apoptotic cells.The r-values were 0.647,0.297,0.259,0.519,-0.299,-0.388,and-0.931,respectively.Conclusions Hyperexpression of miR-21 participated in progression of colon carcinoma probably through down-regulating expressions of PTEN and caspase-9 and up-regulating expression of NF-κB and then suppressing cell apoptosis.

Objective To evaluate the influence of ProTaper rotary nickel-titanium files root preparation on root canal filling . Methods 30 cases of molars pulpitis and 20 cases of premolars pulpitis or periapical periodontitis were equally divided into two groups .The group A(30 cases) used the ProTaper rotary nickel-titanium files in root canal preparation and the group B (30 cases) adopted the common K files modified step-back technique for root canal preparation .The influence of the root preparation by 2 sets of apparatus on the effect of root canal filling ,and the several aspects of the root canal filling time ,the matching degree of main root canal file and master gutta-percha ,proper filling rate ,postoperative reaction of root canal filling were compared for conducting the e-valuation .Results In the mean root canal filling time of the group A ,average premolar time was 4 min 25 s and molar was 1 min 30 s ,however in the group B ,average premolar was 5 min 30 s and molar was 11 min 41 s ,the time in the group A was greatly short-ened with statistical difference(P<0 .05);the matching degree of main root canal file and master gutta-percha in the group A was 86 .37% for premolar and 88 .89% for molar ,however in the B group ,which was 62 .50% for premolar and 26 .67% for molar ,the matching degree in the group A was greatly improved with statistical difference between them (P<0 .05);the proper filling rate of upper premolar from the X-ray film was 72 .73% in the group A and 70 .83% in the group B ,while which of molar was 80 .0% in the group A and 77 .78% in the group B ,here was no statistical difference between them (P>0 .05);the postoperative pain reaction of root canal filling in both groups were very low and weak without statistical difference .Conclusion The ProTaper rotary nickel-titanium files root preparation can accelerate the root canal filling speed ,improve the matching degree of main root canal files and master gutta-percha and simplify the root-filling therapy procedure .

Objective:To study the histogenesis and cell differentiation of desmoplastic ameloblastoma.Methods:The expression of various protein filaments was detected by immunohistochemical stainning in 20 cases of desmoplastic ameloblastoma and the ultrastructure of the tumor was observed in 4 cases.Results:CKH,CK8 and CK19 were positive in spindle-shaped and polygonal epithlium tumor cells.Vimentin was positive in mesenchymal fibrocytes and smooth muscle actin in some mesenchymal cells.Ultrastructural observation found that tonifilaments were in epithelial cells and there were desmosomes between these cells.In stroma myofibroblasts were presented.Loose connective tissue could be seen around some epithelial cell nests.Conclusion:Desmoplastic ameloblastoma may be arise from the early stage of the dental germ formation, epithelial cells of the tumor had the tendency to differentiate to the keratinized cells.

Objective To explore the relationship between the (PON1) A191B gene polymorphism and NIDDM with or without cerebral infarction (CI).Methods With polymerase chain reaction(PCR) technique,we detected PON1 A191B genotypes in 72 healthy controls,42 NIDDM patients without CI and 45 NIDDM patients with CI.Results There was no relationship between PON1 191 gene B polymorphism and the NIDDM patients without CI,but there was remarkable relationship between it and NIDDM patients with CI (0.78,P

BackgroundThere are a lot of studies about the wound healing charateristics of cornea after SBK with femtosecond laser.In our study,mechanical microkeratome was preferred for corneal flap.We observed the proliferation of keratocytes by investigating the myofibroblast ( MF) activity.Objective The study was to compare the morphologic and histological changes in the cornea after sub-Bowmans keratomileusis ( SBK) with photorefractive keratectomy ( PRK) and laser in situ keratomileusis ( LASIK)by investigating the express of transforming growth factor-β( TGF-β)and α-smooth muscle actin( α-SMA)and to investigate the wound healing characteristiCs of cornea after SBK.MethodsTwenty-seven adult New Zealand white rabbits were randomly assigned into group A,B and C.SBK waa performed on the right eyes of each rabbit in group A,LASIK for group B and PRK for group C.All the left eyes were used as the normal control group.Histological examinations by light microscopy were performed on day 7,1 months,3 months after surgery.The expression of α-SMA and TGF-β and the number of activated MF were assessed by immunohistochemiatry.ResultsIn SBK group,corneal epithelium cells proliferation around the wound was seen and the numbers of active fibroblasts were increased after surgery.The expression of α-SMA or TGF-β around the corneal flap and the corneal 8troma started at day 7 postoperatively and peaked at 1 months and decreaaed around the corneal flap and the corneal 8troma started at day 7 postoperatively and peaked at 1 months and decreaaed t3mnh.TFβep( SBK:t=2.226,2.158,2.330,P＜0.05;PRK:t=4.745,6.524,6.293,P＜O.05).The numbersof activ( SBK:t=2.226,2.158,2.330,P＜O.05;PRK:t=4.745,6.524,6.293,P＜0.05).The numbersof activatedMFs were different fromLASIKstatisticallytoobetweenSBKgroupandPRKgroup ( SBK:t =4.439.5.692,4.175,P＜0.05 ; PRK:t=6.330,6.723,5.267,P＜0.05 ).Theα-SMAand TCF-βexpressionsin SBKgroupwerelessthan PRK group but more thanLASIKgroup( TCF-β:t =4.691,5.527,t =4.399,P＜0.05 ; α-SMA:t =9.637.10.282,8.197,P＜0.05).The numhers of MFs in SBK group was less than PRK group before 3 months and were same at 3 months ( t =5.188,4.529,P＜0.05 ).Conclusions ComparedwithconventionalLASIK,SBKcanup-regulatethe expression of α-SMA.TGF-β,activated MFs in the corneal flap.which enhance corneal biomechanics and promote healing.However,most of the disadvantages caused by wound healing in SBK still remain compared to PRK.

Objective:To analyze the epidemiological characteristics of human immunodeficiency virus (HIV) and hepatitis B virus (HBV) co-infected patients in Beijing and investigate the associated factors.Methods:The clinical data of patients with HIV infection who were treated in HIV/AIDS designated hospitals (Peking Union Medical College Hospital, Beijing Ditan Hospital and Beijing Youan Hospital) were retrospectively analyzed.Results:A total of 11 572 patients were finally included in the study, among whom 532 patients (4.6%) were co-infected with HIV and HBV. Most of the co-infected patients were young male adults (28~48 years old), accounting for 85.9%. The main transmission route was homosexual behavior (74.8%). There were 87.4% co-infected patients treated with two anti-HBV drugs, including lamivudine (3TC) and tenofovir (TDF). From 2013 to 2018, the annual prevalence of HIV and HBV co-infection decreased gradually, with the rate of 6.37%, 4.55%, 3.92%, 4.68%, 4.24% and 2.74%, respectively. In our study, The main influencing factors of HIV and HBV co-infection were age older than 28 years old versus<28 years old ( OR=2.807, 95% CI 1.241-6.345) and marriage status (married versus unmarried, OR=1.259, 95% CI 1.004-1.579). Conclusions:The proportion of HBV infection in HIV-infected patients is 4.60% (532) in our cohort. From 2013 to 2018, the prevalence of HIV and HBV co-infection in Beijing shows a decreasing trend. The risk of co-infection is higher in married young adults (28~48 years old).

Objective]To sum up director He Ruoping's feature in recognizing ovarian cancer causa morbi and mechanism and clinical treatment rules; To explore the clinical experience of director He Ruoping treatment with ovarian cancer.[Method]By learning from director doctor He Ruoping for three years and combining my own clinical experience, from the etiology and pat hogenesis, it sums up the teacher He' s unique feature of by stages treatment of ovarian cancer, and with one case for detailed explanation.[Result]Teacher He advocates the combination of traditional Chinese medicine and western medicine, puts forward the principle of ovarian cancer.By the way of strengthening body resistance and eliminating evil, Teacher He adopts the help of invigorating the spleen and tonifying the kidney,supplementing qi and nourishing yin,soothing the liver and regulating qi,clearing away heat and toxic material,promoting blood circulation to remove blood stasis, softening firm scattered nodes,and water permeability wet for the main therapy in treatment of ovarian cancer and the curative effect is remarkable.[Conclusion] To take Teacher He' s experience for treating ovarian canceR from essence,replenishing qi and blood,removing turbidity can significantly relieve patients' clinical symptoms and improve patients' life quality.

Objective To optimize the producing area and parts of Gardenia roots. Methods Oleanolic acid 3-acetate was hydrolysed into Oleanolic acid in Gardenia roots from 10 different origins, and root, stem, leaf of Gardenia from Liuyang Hunan, and the content was determined by HPLC. Results The content of oleanolic acid 3-acetate in Gardenia roots of different origins from high to low was:Shaodong, Liuyang of Hunan>Anji of Zhejiang, Guiyang of Hunan>Ningxiang, Anhua of Hunan, Zhangshu of Jiangxi>Liling, Pinjiang, Youxian of Hunan. The content in root was 2 times of that in stem and leaf. Conclusion Experimental data were provided for the optimization of producing area and part of Gardenia roots.

Purpose To investigate the expression of insulin-like growth factorIImRNA binding protein 3 ( IMP3 ) and microvessel density (MVD) in squamous carcinoma of the cervix (SCC) and analysis their relationship in SCC. Methods The expression of IMP3 and MVD was examined by immunohistochemistry SP method in normal cervical epithelium ( NCE) , low-grade cervical intraepi-thelial neoplasia (CIN-L), high-grade cervical intraepithelial neoplasia (CIN-H) and SCC. Results (1)The positive expression rates of IMP3 in NCE, CIN-L, CIN-H, SCC tissues were 0(0/15), 0(0/11), 37.5%(9/24) and 86.0%(43/50), the difference was statistically significant (x 2 =53.345, P=0.000). IMP3 expression was significant difference among NCE and CIN-H, SCC (P<0.008 3), and that was also among SCC and CIN-L, CIN-H (P<0.008 3). (2)The MVD count was increased with the development of cervical squamous lesion, there was significant difference among those groups (F=145.968, P<0.01), and the difference was al-so statistically significant between every two groups (P<0.05). The positive expression of IMP3 and MVD count in 50 cases of SCC tissues showed statistical difference in different pathologic grade, lymph node metastasis, and depth of tumor invasion groups ( P<0.05), but didn't in different patients’age groups (P>0.05). And the positive expression of IMP3 was closely related with MVD count in SCC tissues (rs =0.323, P<0.05). Conclusion IMP3 plays an important role in the occurrence, infiltration and metasta-sis of SCC, and the abnormal expression of IMP3 may relate with the angiogenesis of tumors.

Objective To investigate the influence of nebulized Pulmieort respules inhalation after endoscopic sinus surgery (ESS)on the expression of epithelial Na~+ channel(ENaC)protein in nasal mucosa. Methods Forty-four patients with nasal polyps undergoing ESS were randomly divided into Pulmieort respules treatment group(n=21,nebulized Pulmieort respules inhalation for 10 d after ESS)and Rhinocort control group(n=23,Rhinoeort aqueous nasal spray for 10 d after ESS).All the patients were performed biopsy of membrane on the residual middle turbinate 14 d after ESS,eosinophils (Eos)and neutrophils(Neu)per hundred inflammation cells were counted under microscope during ESS and after ESS,and the expression of ENaC protein was detected by immunofluorescence assay. Results The percentages of Eos and Neu decreased in two groups after treatment,and the percentage of Neu in Pulmieort respules treatment group was significantly lower than that in Rhinoeort control group(P<0.05).The expression of ENaC protein after treatment in Pulmieort respules treatment group was significantly lower than that in Rhinoeort control group(P<0.01). Conclusion Application of pulmieort respules after ESS can decrease Neu infiltration and inhibit expression of ENaC protein,which can relieve acute inflammation and edema of nasal mucosa.