Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; therapy ; Rhinitis, Allergic, Seasonal ; therapy

China ; Chronic Disease ; Humans ; Phenotype ; Rhinitis ; genetics ; immunology ; Sinusitis ; genetics ; immunology

Asthma ; immunology ; Humans ; Rhinitis ; immunology

Allergens ; immunology ; Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; immunology ; therapy ; Treatment Outcome

AIM: Pancreatic and duodenal homeobox gene 1(pdx-1) is a crucial transcription factor in pancreatic islet development and differentiation. This study was conducted to evaluate whether pdx-1 delivered by adeno-associated virus (AAV) could induce liver cells to differentiate into insulin-producing cells in diabetic rats and thus provide more information for cell replacement therapy for diabetes. METHODS: Recombinant AAV vector was employed to deliver pdx-1to STZ-induced diabetic rats via portal vein (4×1011). Blood glucose and body weight were monitored. Gene expression of pdx-1 and insulin were determined by RT-PCR and immunocytotochemistry (ICC) at the 6th week after the injection. RESULTS: AAV-pdx-1 group showed obvious gene expression of pdx-1 and insulin by RT-PCR analysis and the presence of more insulin-positive cells by ICC. Hyperglycemia was partially ameliorated and body weight was also increased in AAV-pdx-1 treated diabetic rats, though still significantly different from those in the non-diabetic group. CONCLUSION: The data indicate that AAV-pdx-1 can induce more rat liver cells into insulin-producing cells in vivo, thereby ameliorate hyperglycemia. Further experiments are needed to explore which subpopulation of liver cells responds to this development shift and the mechanism of this development shift induced by pdx-1 in order to improve the differentiation efficiency.

Objective　To study the effect of rehabilitation training on the expression of Fos in the central nervous system(CNS) of rats with cerebral infarction. Methods　60 SD rats were made as cerebral infarction models and divided into 2 groups at random 24 hours later: rehabilitation group was given balancing, grasping, rotating and walking and other trainings everyday, while immobiligation group was fixed in cages. Immunohistochemistry was used to detect the Fos expression 1 d, 7 d, 14 d, 21 d and 28 d after infarction respectively. Results　Fos-positive immune reaction appeared in cortex,thalamus, hypothalamus, reticular form and posterior gray horn of the spinal cord of both groups　.Rehabilitation group responded stronger than the immobilization group significantly (P<0.01).Conclusion　Rehabilitation training can activate cortical neurons around the infarted focus and in its opposite side of the CNS, and thus promote the recovery of rats' motor functions.

Female ; Humans ; Infant ; Male ; Ultrasonography ; Urachus ; abnormalities ; diagnostic imaging

Objective The study aimed to investigate the effect of lipoxin A4 (LXA4) on lipopolysaccharide (LPS)?induced oxidant stress in human renal tubular epithelial cells (HK2 cells) and possible underlying mecha?nisms. MethodsHK2 cells were divided into three groups: Control ,LPS and LPS+LXA4 groups. After cells were treated with indicated conditions,morphological changes were observed. The expressions of Nrf2 were detected by immunofluorescence and cells were collected for RT?PCR experiments.Results HK2 cells seemed disrupted and necrotic with the administration of LPS. However ,LXA4 could prevent cells from injury induced by LPS. LPS decreased Nrf2 expression and promoted it to translocate to cytoplasm ,while LXA4 could increase its expression and promote it to translocate to nucleus. Moreover ,LPS could decrease Nrf2 and its downstream molecule mRNA expressions,but LXA4 could reverse this effect. Conclusion Our results demonstrated that LXA4 effectively inhibit?ed HK2 cell oxidant stress via Nrf2 pathway.

Objective The turnover intention and the work states of the health technicians were investigated to analyze the influencing factors of turnover and the relationship between turnover intention and work state,to guide the health technicians who want to dimission to make a correct decision,and to make some suggestions to improve their work states.Methods Multistage stratified cluster random sampling method was adopted to survey 2903 health workers on the job of all the county-level public hospitals in Shandong Province by the questionnaire of the Fourth National Health Services Survey.Analysis methods included Two Logistic classification regression and three evaluation methods based on two weights.Results 32.4 percent health technicians had turnover intention.The risk factors of the turnover intention were as follows:medical department,bad assessments of the practice environment,low degree of the diversity of job skills,small level of improving the individual's capacity,low degree of job satisfaction,high degree of job autonomy,high requirement for the expression of individual emotions,high working pressure,high job burnout.The higher turnover intentions were,the lower synthetical evaluation scores of the work states were.Conclusion The county hospitals ought to pay more attention to the health technicians with high tumover intention,and to guide the health technicians who want to dimission to make a correct decision,and to make some suggestions to improve their work states through having a heart-to-heart talk,training,and career planning,etc.

Objective To evaluate the association between gene polymorphism of aldosterone synthese gene CYP11B2(-344T/C)and essential hypertension and to observe the antihypertensive response to Valsartan in Han nationality in Beijing.Methods Polymerase chain reaction was used to detect the polymorphism of aldosterone synthese gene CYP11B2(-344T/C)in 345 hypertensive patients(EH)and 157 normotensive controls(NE)from Aug.1999 to Oct.2003.Clinical blood pressure and biochemical index were also detected.Blood pressure was repeated in 98 patients with Valsartan treatment for 4 weeks.Results The frequencies of CC+CT genotype of aldosterone synthese gene CYP11B2(-344T/C)polymorphism among EH was significantly higher than that among NE(P