Objective To explore the effects of curdione on cognitive function and expression of inflammatory factors in the hippocampus after partial hepatectomy in aged mice.Methods The animals were divided into control group, sham group, operation group, and high-, medium-and low-dose curdione groups.The model of partial hepatectomy in aged mice was established according to the method reported in literature.The levels of SOD, MDA, CAT and GSH-Px in the hippocampus were detected by enzyme-linked immunosorbent assay (ELISA), while the expressions of NF-κB, IL-1β, IL-6 and TNF-α were measured by Western blot.Results The escape latency was significantly increased (P<0.01), and the number of platform-site crossovers was significantly decreased in operation group (P<0.05).Compared with those in operation group, curdione significantly decreased escape latency and increased platform-site crossovers (P<0.05, P<0.05).The levels of SOD, CAT and GSH-Px in the hippocampus were significantly decreased (P<0.05) and the content of MDA was significantly increased after partial hepatectomy (P<0.01), and these were reversed by curdione (P<0.05, P<0.05, respectively).The expressions of NF-κB, IL-1β, IL-6 and TNF-α protein were significantly inhibited by curdione (P<0.05).Conclusion Curdione can significantly improve cognitive dysfunction after partial hepatectomy in aged mice and the mechanism may be related to its inhibition of oxidative stress, inhibition of NF-κB activation and reduction of inflammatory cytokines IL-1β, IL-6 and TNF-α in the hippocampus.

Acute Disease ; Adult ; Age Factors ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; prevention & control ; Pesticides ; poisoning ; Poisoning ; epidemiology ; mortality ; prevention & control ; Rural Population ; Sex Factors ; Survival Rate

Objective To elucidate the possibility of diagnosing BOO with urodynamic data with indefinite P F findings in patients with BPH. Methods Some urodynamic data besides the P F fin dings were studied in BPH patients,and these were compared between the BOO group and the non BOO group. Results There were 252 patients with BOO and 52 patients without.200 patients had no definite P F findings.Several other parameters were compared between the BOO group and the non BOO group.The rate of prostatic plateau was 87.3% and 53.8%, respectively( P

7-year-program clinical medicine is a major that fuses master with bachelor degree education and has higher demand in the integrated ability training.It is in 2002 that Southeast University first admitted students of this major,which,therefore,is brand new challenge to every educators,including EMP teachers.Thus,we initiated teaching innovation and put it into practice,which proved beneficial.

Objective To define whether correlation exists between the enlargement of lateral ventricles and motor development delay. Methods Motor development was assessed with Peabody Developmental Motor Scale (PDMS) in 28 children with cerebral palsy. Correlation between computerized tomography (CT) findings of enlargement of lateral ventricles (ventricle index,body index,width index and frontal horn index) and motor quotients of PDMS(GMQ,FMQ and TMQ) was analyzed. Results Width index was correlated with all three motor quotients (GMQ,FMQ and TMQ)( P

Objective To study the combined toxic effects of fluoride and aluminum on the liver of mouse and the probable mechanism. Methods Kunming mice were randomly divided into nine groups and treated with fluoride and aluminum through drinking water:control(tap water)group, 50 mg/L fluoride group, 150 mg/L fluoride group, 200 mg/L aluminum group, 600 mg/L aluminum group, 50 mg/L fluoride+200 mg/L aluminum group, 50 mg/L fluoride+600 mg/L aluminum group, 150 mg/L fluoride+200 mg/L aluminum group, 150 mg/L fluoride+600 mg/L aluminum group. After 24 weeks of treatment, the activity of ALT, AST, the content of TBIL, DBIL, IBIL, ALB, Ca, P and Mg in the serum was determined. Results Different doses of single fluoride or fluoride+aluminum led to significant different changes in the liver function(single fluoride:F=2.61, P0.05). Compared with the control group, the Ca level in the serum decreased in the exposed groups except 150 mg/L fluoride+600 mg/L aluminum group(P0.05). Conclusion The combination of fluoride and aluminum at certain levels has a toxic effect on the liver, but the combination of fluoride and aluminum at high doses shows an antagonistic effect.

Objective To evaluate the significance of the expression CDK4 and CyclinD1 in renal carcinoma. Methods Real-time fluorescence quantitative PCR method was used to detect the expression level of CDK4 and CyclinD1 in 30 specimens of renal carcinoma tissue and 30 specimens of carcinoma-adjacent renal tissue. Results The expression level of CyclinD1 and CDK4 gene in renal carcinoma tissue was higher than that in carcinoma-adjacent renal tissues (P

Aim To study the anti-tumor activity of PQEP and it s immunomodulation effect.Methods The pharmacological activity of PQS was measured using mice transplanted H22 and MFC tumor. Tumor-inhibiting a ctivities, weight of immune organ, nature killer cells activity and lymphocyte p roliferation ratio were investigated.Results PQEP showed antitumo r and immunomodulation activities in different degrees.Conclusion The anti-tumor activity and immunomodulation are closely linked. The anti- tumor effect of PQEP due to activation of the immunocompetence of the body.

Aim This research was performed to study the anticancer effects of Panax quinquefolium effective parts (PQEP) in human chronic myeloid leukemia (K562). Method After K562 cells were routinely cultured,MTT assay was performed for cytotoxicity test. Cytotoxicity of PQEP (6.25～400 mg?L -1) in K562 cells was increased in a dose-and time-dependent manner. To explore the mechanism of cytotoxicity, we used several measures of apoptosis to determine whether these processes were involved in PQEP-induced leukemic cell death.Results PQEP induced the cell shrinkage, cell membrane blebbing, chromosomes condensation and DNA fragment. In addition, the flow cytometric analysis revealed PQEP (10～400 mg?L -1) dose-dependently increased apoptotic cells with hypodiploid DNA contents. Conclusion These results indicate that PQEP can control leukemic K562 cells through apoptosis and may have a possibility of potential anticancer activities.

0.05).There was a significant positive correlation between serum osteocalcin and ESR in female patients with RA aged less than 50 years (P