Objective To explore the intervention of erythromycin(EM) on acute lung injury(ALI) in rat model MethodsALI rat model was established by intravenous i njection of oleic acid(OA) EM of 180 mg/kg was injected intraperitoneally t wo h ours before OA challenge in the prevention group and consecutive three days afte r OA challenge in the treatmen group ResultsCompared with con trol group,the aninmals in prevention group and treatmen group had lower lung extent,milder pathomorphological changes and pathologic integral The concentra tion of protein and the numbers of neutrophils and lymphocytes in bronchoalveola r lavage fluid(BALF) were also reduced ConclusionEM may part ly prevent the development of ALI by inhibiting the neutrophil and lymphocyte ac cumulatation in the lung

【Objective】To observe the effect of treating psoriasis vulgari(PV)by solving disorders of blood system.【Methods】Two hundred and six patients with PV were randomized into groups A and B.Group B(n=70)was treated with oral use of clarityne,vitamin C,Tripterygium Glycosides Tablets,and roxithromycin,as well as external application of sulphur ointment or clobetasol propionate ointment.Group A(n=136)was additionally treated with basic recipe(composed of Rhizoma Smilacis Glabrae,Radix Isatidis,Folium Isatidis,Radix Rehmanniae,Cortex Moutan,Herba Hedyotis Diffusae,Herba Lobeliae Chinensis,Nidus Vespae,Rhizoma Chuanxiong,Rhizoma Alismatis,Herba Plataginis,Fructus Kochiae,Cortex Dictamni,Radix Glycyrrhizac).Meanwhile,patients in group A were classified into 3 syndrome patterns of blood heat,blood stasis and blood dryness,and treated with corresponding recipes.After three courses of treatment(3 month in total),the therapeutic effect,as well as the side effects and recurrence rate was evaluated in the two groups.【Results】In group A,71 patients were cured,22 markedly effective,26 effective,17 ineffective and the total effective rate was 87.50%,and 23,15,21,11 and 84.28% in group B,respectively.The therapeutic effect in group A was superior to that in group B(P

Objective To observe the clinical curative effect of Xiaoer Chiqiao Qingre granule in the treatment of infantile upper respiratory tract infections.Methods 100 children with upper respiratory tract infection were selected,and they were randomly divided into observation group and control group,50 cases in each group.The observation group was treated with Xiaoer Chiqiao Qingre granule,the control group was given ribavirin effervescent granules.The effects of the two groups were observed.Results The total effective rate of the observation group was 96％,which was significantly higher than 78％ of the control group (x2 =9.500 6,P ＜ 0.05).The fever,symptoms and signs of improvement time in the observation group were better than those in the control group.Conclusion Xiaoer Chiqiao Qingre granule in the treatment of infantile upper respiratory tract infection can defervesce quickly,and has quick curative effect,so it is worthy of promotion.

0.05);the onset time periods of the 3 groups were(1.9?1.1) min,(3.6?2.1) min,and(3.3?1.5) min,respectively,with that of group A significantly shorter than those of B and C groups(P0.05).The glottis exposure degree in group A was significantly better than that of B and C groups(P0.05).Conclusion:Rocuronium is superior to cisatracurium in induction intubating for general anesthesia in terms of clinical effect time and glottis exposure degree,and it can provide better intubating conditions.

Objective To observe the changes in contents of ? 1 Microglobulin (? 1 M) in urine after exposure to acceleration of different speeds(+Gz) in order to provide scientific indications for efficient protection for pilots under high +Gz acceleration and efficient flight health service support. Methods Six healthy men were subjected to the following experiments: ①pressurized anti G suit (KH X); ②pressure breathing for +Gz (PBG); ③pressurized anti G suit and PBG and AGSM (up to +9Gz for 10 s). Urine samples were collected before, immediately after, 30 minutes and 24 hour after the exposures for the determination of ? 1 microgolbulin (? 1 M). Results It was found that ①The G tolerances of subjects under 3 conditions were (6 25?0 52) G/10s, (8 17?0 26) G/10s, 6 5G/30s, and 9 00 G/10s, respectively. ②30 minutes after each series of exposure, the contents of ? 1 M in urine samples were increased ( P

[Objective]To explore a method for isolating and culturing bone marrow mesenchymal stem cell(BMSCs) in vitro,and improve the cellular conglutination ability of biomaterial poly-lactide-co-glycolic acid(PLGA),and observer the adhesion of BMSCs cultured on PLGA modified with Ⅱ collagen.[Method]BMSCs were isolated and cultured in vitro.The cell exterior antigen,cell livingness and cell cycle were analyzed by flow cytometry method,and cellular configuration was observed continually under invertd phase-contrast microscope.PLGA was made by phase separation,and was modified with Ⅱ collagen.The third era BMSCs were cultured on PLGA,and its adhesion with biomaterial was observed scan electron microscope.[Result]BMSCs could be isolated and cultured in vitro,and express CD29,CD44,CD106,but not express CD34,CD45.The cell livingness was 88.96%,cells in G0-Glperiod are 90.32%.The cell morphology was spindle.The average diameter of PLGA modified with Ⅱ collagen was 100 ?m,and PLGA has good adhesion with BMSCs.[Conclusion]BMSCs could be cultured stability in vitro for long time,and is good seminal cell of tissue engineering.Ⅱ collagen can improve the cellular adhesion of PLGA,PLGA modified with Ⅱ collagen is the good biomaterial of tissue engineering.

Objective To establish a UV spectrophotometry method for determination of diphenylamine in air of workplaces.Methods The air samples were collected by glass fiber filter treated with sulfuric acid,desorbed with alcohol by ultrasonic for 10 min,determined by UV spectrophotometry.Results The detection range of the method was 0.19-60 mg/m3(the air volume was15 L).The regression equation:y=0.13x-0.016.The correlation coefficients was 0.999 9.The detection limit was 0.28 ?g/ml.The relative standard deviations was 1.9% -8.4%.Sample-collected efficiency was 99%,desorption efficiency was 98% and the penetration capacity was more than 2.2 mg.Sample stability was 10 days at least by being stored in a refrigerator at 4℃ or by ambient storage.Conclusion This method is applicable to the determination of diphenylamine in air of workplaces.

Objective: To eliminate the noise of the plethysmography wave and obtain plethysmography wave signal without noise. Methods:Using sym4 and combining the improved wavelet threshold wavelet algorithm to remove high frequency white noise of plethysmography wave, and then using spline differential fitting method to remove baseline drift of pulse wave volume. Results:The experimental results show that the high frequency noise and baseline drift of plethysmography wave have been removed effectively processed by the sym4 wavelet and combining with improved threshold of wavelet algorithm and spline difference after fitting. Conclusion:The method can well remove the noise of the plethysmography wave and retains the effective information of plethysmography wave effectively.

T cell immune deficiency results in inefficient anti-tumor immune response in most patients with hematological malignancies,thereby tumor cells escaping the attack of host immune system,that is the immune escape of tumor cells.Recently,new insights regard to the CD8+ T cell exhaustion which is one of the most important mechanisms of tumor immune suppression.The CD8 + T cell exhaustion is mediated by abnormal expression of T-cell immunosuppressive receptors such as PD-1 and CTLA-4.Blocking these molecules may restore the partial or all functions of T cells.This article reviewed the advances on the role of the PD-1 and CTLA-4 in hematological malignancies,including their functions on mediating T cell-immune tolerance and the progression of targeted immunotherapy,to provide the new strategy for hematological malignancies.

Objective To explore the characteristics of the reperfusion arrhythmia after acute myocardial infarction (AMI) pa-tients underwent emergency coronary interventional therapy ,in order to reduce the incidence of serious arrhythmia ,and improve the prognosis and quality of life .Methods Clinical data of 147 patients from January 2009 to May 2012 with acute myocardial infarction occurrence reperfusion arrhythmia after underwent emergency coronary interventional treatment were analyzed .Results In 147 ca-ses ,62 cases of reperfusion arrhythmia occurred .Infarct-related artery for the left anterior descending branch (LAD) 33 patients (LAD group ) ,left circumflex artery (LCX) 7 cases (LCX group ) after surgery ,the right coronary artery(RCA) 22 cases (RCA group) .The tachyarrhythmia in LAD group was higher than RCA group(P<0 .05);the bradyarrhythmia in RCA group was higher than LAD group (P<0 .05) .Opening of the infarct-related artery<6 h arrhythmia had 43 cases occurred ,and >6 h arrhythmia had 19 cases occurred ;Arrhythmia after acute myocardial infarction occurred mainly in infarct related artery opened earlier .Conclusion AMI occurrence reperfusion arrhythmia after underwent emergency coronary interventional treatment have high incidence ,there is a correlation between the arrhythmia type and infarct-related artery ,also has a correlation between incidence and reperfusion time .