This is a review of recent progress in the study on environment sensitive hydrogel based on cyclodextrin and their most recent and relevant applications in the intelligent drug delivery systems. Based on relevant literatures, the development of environment sensitive hydrogel responsive to physical, chemical and biochemical stimuli was introduced, involving their categorization, design principles, mechanism of action and potential application. Various new types of intelligent drug delivery system, which responds to various triggers, could be constructed by using the cyclodextrin-based environment sensitive hydrogel. They made it possible to control the drug release freely. Although these hydrogels are still at their research stage, they have attracted considerable interest in the intelligent drug delivery system.

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is characterized by non-cardiogenic,acute,progressive and hypoxic respiratory insufficiency or respiratory failure mediated by various internal and external stimuli.The pathogeny of lung injury is varied,pathogenesis is complicated,and it is not clear.Related pathogeny and pathogensis of lung injury is reviewed as follows.

Adenoviridae ; genetics ; Genes, p53 ; Genetic Therapy ; methods ; Genetic Vectors ; Humans ; Neoplasms ; therapy ; RNA Interference ; Tumor Suppressor Protein p53 ; genetics

Anticancer drugs resistance is often ascribed to gene mutation,amplification or epigenetic changes that affect the uptake,metabolism or export of drugs at the cell level.Tumor physical microenvironment not only influences the drug distribution but also causes difference in cell proliferation rate and hypoxia or acidosis due to its heterogeneity,which could affect the tumor drug resistance.This review discusses the relationship between cancer physical microenvironment and drug resistance and the strategies for enhancement of the chemotherapy sensitivity through modification of the tumor physical microenvironments,as well as foreground of the research.

OBJECTIVE:To observe the safety and immunological effects of Measles-mumps-rubella(MMR)attenuated live at-tenuated vaccine in children. METHODS:300 children aged 8-12 months receiving inoculation were selected from Changsha Hospi-tal for Maternal and Child Health Care during Jan. 2015-Apr. 2016 to observe safety and immunological effects. Those children were divided into MMR group,measles vaccine group,mumps vaccine group and rubella vaccine group according to vaccine type, with 75 cases in each group. The occurrence of ADR in 72 h were compared among 4 groups after inoculation;venous blood of chil-dren was collected before vaccination and 5 months after vaccination,and the antibody positive test was carried out by micro coagu-lation inhibition (HI) test;HI antibody titer was recorded after immunization,and positive rate and genometric meantiter(GMT) were calculated. RESULTS:The incidence of ADR in 4 groups were 9.33%,8.00%,8.00% and 10.67%,respectively. No local ADR was found in 4 groups;among systemic ADR,the incidence of fever was higher than that of other clinical manifestations,be-ing 4.00%,4.00%,4.00% and 5.33%;there was no statistical significance in the incidence of ADR among 4 groups(P>0.05). Measles,mumps and rubella antibody positive rates of MMR group were 100%,92.00% and 100%,respectively;antibody posi-tive rates of measles vaccine group,mumps vaccine group and rubella vaccine group were 100%,85.33% and 100%,respective-ly;there was no statistical significance in same antibody positive rate among 4 groups(P>0.05). GMT of measles in MMR group and measles vaccine group were 1∶41 and 1∶27,that of mumps in MMR group and mumps vaccine group were 1∶6.3 and 1∶6.2, there was no statistical significance (P>0.05);GMT of rubella in MMR group and rubella vaccine group were 1∶320 and 1∶849, with statistical significance (P<0.05). CONCLUSIONS:Compared to traditional single vaccine,MMR dose not increase the inci-dence of ADR and not influence positive rate,but GMT of rubella increases significantly,to which should be paid attention.

Atherosclerosis ; Blood Vessels ; cytology ; Humans ; Stem Cells

The cerebral resuscitation is the most important aim in advanced cardiopulmonary cerebral resuscitation (CPCR). Cerebral function protection after cardiac arrest (CA) is important to improve survival rates including those after the discharge. Therapeutic mild hypothermia maybe the only method that can improve neurological function of patients following resuscitation after CA, which was recommended as one of treatment strategies for unconscious patients after successful resuscitation in 2010 American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Although there are many cooling methods of inducing hypothermia, each has some advantages or shortcomings. There were many controversies on the choice, which need further research. We make a summary and analysis about the mechanism of therapeutic mild hypothermia, the possible complications at different stages and the clinical application of mild hypothermia, such as the evaluation before therapeutic mild hypothermia and the choice of hypothermia protocol and cooling methods, to provide guidance for clinical mild hypothermia therapy.

AIM: To explore whether human telomerase reverse transcriptase (hTERT) antisense oligodeoxynucleotide (ASODN) could enhance the sensitivity of acute lymphoblastic leukemia cells from relapse patients to cisplatin. METHODS: The expression levels of hTERT protein were detected by immunofluorescence using fluorescence isothiocyanate (FITC), the number of viable cells was determined using the trypan blue dye exclusion assay, and apoptosis was detected by morphological observation and flow cytometric cell cycle analysis. RESULTS: The expression of hTERT protein was inhibited after treatment with hTERT ASODN. Treatment with cisplatin combined with hTERT ASODN had significantly reduced the number of viable acute lymphoblastic leukemic (ALL) cells (P<0.05). In morphological observation of apoptotic cells using Hoechst33258 and PI double staining techniques, cells displayed classic apoptotic changes in the presence of cisplatin or cisplatin combined with hTERT ASODN or ASODN at 48 h. Apoptotic rates of cells treated with cisplatin and ASODN were higher than that of cells treated with cisplatin alone (P<0.05). CONCLUSION: hTERT ASODN could increase sensitivity of cultured primary acute lymphoblastic leukemia cells from relapse patients to cisplatin.

Objective To investigate the value of 3D rapid prototype technology in therapy of complex pilon fractures.Methods In this study,60 patients with pilon fracture who had been hospitalized during January 2013 to May 2014 were randomly and equally divided into a 3D group and a control group according to their hospitalization order.The 3D group:21 males and 9 females,an average age of 34.8 ±6.0 years,13 cases of AO type C2 and 17 ones of AO type C3.The control group:21 males and 9 females,an average age of 35.8 ±6.2 years,12 cases of AO type C2 and 18 ones of AO type C3.The 3D group received surgery aided by rapid prototype and 3D printing while the control group underwent conventional surgery with open reduction and internal fixation.In the 3D group,the distal tibia models were manufactured by 3D rapid prototyping technology based on the 3D reconstructions which were accomplished by computer aided technology.The diagnosis,classification and preoperative plan were then conducted using the models.The 2 groups were compared in terms of operation time,postoperative skin necrosis and infection,and Maryland foot function scoring at the last follow-up.The 2 groups were compatible in preoperative demographic data (P ＞ 0.05).Results There were significant differences between the 3D and control groups in operation time (65.1 ±4.8 minutes versus 80.5 ± 3.6 minutes) and rate of postoperative complications [6.7％ (2/30) versus 16.7％ (5/30)] (P ＜0.05).There was no significant difference between the 2 groups in the excellent to good rate by Maryland foot function scoring at the last follow-up [76.7％ (23/30) versus 73.3％ (22/30)] (P ＞ 0.05).Conclusion Since rapid prototype and 3D printing technology can display the morphology of pilon fracture intuitively and stereoscopically,it helps surgeons with accurate assessment of the fracture.3D printed models of the pilon fracture pre-operatively raise the accuracy of reduction,reduce both operation time and incision complications,and facilitate functional recovery.

A Review] The oncogene expression and growth of leukemic cells could be inhibited by antisense oligonucleotide. The selection of target genes is the key step in the research of antisense oligonucleotide on leukemia. The article would review the status and prospect of some target genes of leukemia in the investigation of antisense oligonucleotide.