The mental health state of the 256 fourth-year medical students of Kunming Medical College was preliminarily analyzed by requiring. The students fill in the inquiry sheets (questionare) in a question-and-answer way. The ages of these 136 male and 120 female students ranged from 19 to 23 years old, and the self-desiged 109 items of the investigation were induced into 8 aspects related to the mental health such as the outlook on life etc. The result showed that these are differences between the mental health state of the senior medical students and the traditional ideas, morality, manners of education etc. Some initial points of view were put toward.

Glucosylceramide synthase(GCS) plays an important role in ceramide metabolism and can block apoptosis signal pathway which is mediated by ceramide. Recently, studies have demonstrated that overex-pression of GCS exists in various muhidrug resistant cancer cell lines and is related to drug resistant pheno-type. A possible connection between GCS and P-gp is also suggested. Moreover,the level of GCS can be down-regulated to increase tumor cells sensitivity by chemical inhibitor and RNA interference. Thus, inhibition of ac-tivity and expression of GCS is a new mechanism of reversing muhidrug resistance.

Protracted bacterial bronchitis (PBB) is a common cause of children chronic cough,especially in those aged 1 ～ 6 years.It is defined by the presence of persistent wet cough for longer than 4 weeks,but lacks of symptoms or signs of other alternative diagnosis of chronic cough.It shows a normal or near-normal chest radiograph,a intense neutrophilia in bronchoalveolar lavage fluid(BALF),and a chronic purulent bronchitis under bronchochoscopy,and usually resolves after two-week course of appropriate oral antibiotic (usually amoxicillin-clavulanatc).So far,PBB hasn't been fully recognized by most pediatricians,and often been unrecognised or misdiagnosed as asthma.In this paper,pathogenesis,diagnosis and treatment are reviewed.

Objective To explore the protective effect of Ligustrazine on pulmonary fibrosis caused by hyperoxia in neonatal rats.Met-hods Eighty Sprague-Dawley(SD)clean neonatal rats,which were less than 12 hours old,were enrolled in this study.The neonatal rats were randomly divided into 4 groups:air-exposed control group(group A);air-exposed plus Ligustrazine-treated group(group B);hype-roxia-exposed control group(group C);hyperoxia-exposed plus Ligustrazine-treated group(group D).The neonatal rats of group B and group D were intraperitoneally injected with Ligustrazine[30 mg/(kg?d)] in solution of normal saline(NS),group A and group C were intraperitoneally given the same dose of NS.On 14th day,10 rats of each group were randomly chosen and then killed,the pulmonary pathological changes were observed by HE staining under light microscope.The area percentages of collagen fibers were measured by Masson staining and imaging analysis.The degree of pulmonary fibrosis was evaluated by the area percentages of collagen fibers and hydroxyproline content.The results were analyzed by SPSS 13.0 software.Results On the 14th day of the exposure,the histopathologic examination demonstrated broadened lung interstitium and reduced alveolar quantity and lung fibrosis were developed in group C;Ligustrazine treatment obviously alle-viated the changes of lung histomorphology.The newborn rats in group C had lower body weight compared that of with group A,B(Pa0.05).Conclusion Early use of Ligustrazine can decrease the severity of the pulmonary fibrosis of neonatal rats exposed to hyperoxia.

With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.
Bioengineering ; Industrial Microbiology ; Pichia

Objective:Primary nodal and extranodal diffuse large B-cell lymphoma(DLBCL)were investigated for the heterogeneity of histopathology and immunophenotype,and their relation to clinical stage,comparatively.Whether E2F1 can be used as a germinal center B cell(GCB)DLBCL marker was also discussed.Methods:Classification of histopathology and immunophenotype of 98 cases were studied by immunohistochemistry in tissue microarray.Results:Histopathologic morphology presented as:centroblastic(CB,88.8%,87/98),immunoblastic(IB,5.1%,5/98),anaplastic(ALCL,3.1%,3/98),and T cell rich B cell lymphoma(TCRBCL,3.1%,3/98).Of which,31 cases were GCB DLBCL,10(20.8%,10/48)nodal,and 21 extranodal(42%,21/50,P=0.024).The rates of Stages Ⅰ/Ⅱ in nodal and extranodal area were 48.5% and 70%,respectively(P=0.015).The rate of Stage Ⅰ/Ⅱ in GCB DLBCL(74.2%)were higher than in non-GCB DLBCL(50.7%,P=0.029).The CD10 positive rates were 36.8% and 17.1% in Stages Ⅰ/Ⅱ and Ⅲ/Ⅳ,respectively,and had significant differences(P=0.033).The CD10 positive rates were 18.8% and 38% in nodal and extranodal area,respectively(P=0.035).The positive rates of E2F1 were 38.8% and 16.5% in GCB and non-GCB DLBCL,respectively,and had significant differences(P=0.016).The positive rate of E2F1 had positive relation with the expression of CD10 and Bcl-6(P

Objective To evaluate the clinical and pathological features of drug-associated acute interstitial nephritis. Methods Clinical presentations and pathological features were investigated in 14 patients with drug-associated acute interstitial nephritis. Lymphocyt; stimulation test was performed to confirm, the offending drugs. Results All patients presented with acute renal failure, but typical clinical features were often absent. Renal biopsy was therefore needed to establish the diagnosis. Conclusion Lymphocyte stimulation test is a very useful means in the determination of offending drugs.

Objective To study the efficacy of suction combined with ligation and injection of Xiaozhiling in treatment of internal hemorrhoids. Methods 244 patients with internal hemorrhoids were treated using suction combined with ligation and injection of Xiaozhiling,and followed up for 3～6 months after treatment. Results 236 cases were cured,and the cure rate was 96.7% ;244 cases were effective,and the effective rate was 100%. Conclusion Treatment with internal hemorrhoids using suction combined with ligation and injection of Xiaozhiling was the ideal way to treat internal hemorrhoids. This method was more effective and safer than the traditional way, with the advantages of shorter time of inpatient stay and earlier return to normal activity with no harm and suffering.

Purpose: To observe the curative effect of acupuncture on female climacteric syndrome.Methods: Guanyuan (CV 4), Zhongji (CV 3), Zigong (Ex-CA 1), and Sanyinjiao (SP 6) were selected as main acupoints to treat 30 cases of female climacteric syndrome according to TCM principle of aiming mainly at the kidney in the treatment of this disease. Results: The total effective rate from acupuncture treatment of this disease was 90%. It was found in the patients that serum endocrine hormones changed significantly after acupuncture as compared with before it (P＜0.05). Conclusion: It is preliminarily considered that acupuncture has a comprehensive regulating effect on neuroendocrine system and can restore the dysfunctional vegetative nervous and endocrine systems to a stable state,removing or reliving various clinical symptoms in women's climacteric.

MicroRNA (miRNA) is a class of endogenous non-coding RNA which found in eukaryotes and has regulatory function.Its size is about 20 to 25 nucleotides.Recent studies found that miRNA is closly related to the EBV,LMP1,signal transduction pathway,tumor-associated gene network,cell mitosis,tumor angiogenesis,tumor invasion and metastasis of nasopharyngeal.Therefore,studing the relationship between miRNA and nasopharyngeal will help to deepen the understanding of its pathogenesis,and also will provide new inspiration and basis for clinical diagnosis and treatment.