Objective To investigate the role of Epstein-Barr virus(EBV) infection on the abnormal activation of peripheral B-iymphocytes of systemic lupus erythematosus (SLE) patients,and the relationship between EBV infection and the abnormal activation of B-lymphocytes and SLE activity is explored. Methods BamH I-W genome in EBV DNA of 52 SLE patients and 23 controls were examined by PCR-Southern blotting. The level of ANA and EBV VCA-IgM in sera was measured by ELISA. The expression of CD19+ and expression rate of CD19+ and CD23+/CD19+ in EBV-positive SLE patients was higher than that of EBV-negative patients. Meanwhile,the fluorescence signal of CD23 in B cell of EBV-positive SLE patients was stronger than that of EBV-negative ones. Conclusion EBV infection may be related to abnormal activation of B-lymphocytes.EBV may infect and activate B-lymphocytes by up regulating the expression of CD23 of B-lymphocytes. The abnormal activation of B-lymphoeytes is associated with SLE activity.

This paper analyzes the causes and influences of difficult registration from the perspective of ethics , and proposes solutions to the problem of difficult registration , and leads patients to see the doctor scientifically and rationally , and insists on “people first” and enhances hospital management .The government should perform its responsibilities , effectively protect the equal right of citizens to see the doctor and make concerted efforts to solve the problem of difficult registration .

In ethical perspective , the author analyzed the medical cause of violence and influence , and propo-ses ways to prevent medical violence is:advocating the spirit of medical humanities , implementing humanistic care;to strengthen the construction of hospital ethics system , improve medical service quality;to implement the functions of the government , maintaining the authority of the law; to strengthen the construction of citizen moral ethics and social responsibility together , in order to jointly build the doctor -patient harmonious medical environment .

After the commonly used domestic interrogation mobile medical APP were classified, the functions of different APP were analyzed according to the experiences of their users, and their development was prospected.

Objective:To detect the relation of peroxisome proliferators actived receptor ?(PPAR?)in the hepatic tissue of chronic hepatitis B and explore its significance.Methods:The protein and mRNA expression of PPAR? was detected by means of immunohistochemistry and semi-quantitative RT-PCR in cases of hepatic fibrosis staging S0-S4 of chronic hepatitis B and cases of hepatic hemangioma.The average of A values was obtained by image analysis.Results:There was not obvious correlation between PPAR? level and the hepatic activity index.Positive staining of PPAR? was observed in all of the human liver tissues.The expression of PPAR?in tissues of staging S0 and normal liver was stronger than that in tissues of staging S3 and staging S4(P

Objective:To investigate the effect of preoperative intraarterial infusion chemotherapy on the expression of Smad4 in colorectal carcinoma.Methods:15 cases of advanced colorectal carcinoma were treated with preoperative intraarterial infusion chemotherapy,18 cases treated with systemic chemotherapy and 21 cases treated with operation served as the control group.The expression of Smad4 was detected by immunohistochemic S-P method.Result:Among the three groups,the expression level of Smad4 gradually increased(P

Objective:To investigate the clinical features of renal cell carcinoma(RCC) in young adults for improving its diagnosis and treatment.Study was dome on 31 cases at 9 male and 22 female patients were admintted in our hospital. Methods:A total of 447 cases of RCC admitted in our hospital from January 1990 to December 2006 were retrospectively studied.Among them,the clinlical data of 31 cases of RCC in young adults were analyzed and compared with those of the common RCC patients. Results:Of the 31 cases,9 cases were male and 22 cases female,with the ration as 1∶2.44.Tumors were discovered incidentally in 10 cases(32.3%) and symptomatically in 21 cases(67.6%);there were 3 cases(9.7%)of small renal cell carcinoma and the carcinoma diameter of the other 28 cases were beyond 3cm. It had no significant difference when comparing the 5-years survial rate of renal cell carcinoma in young adults(53.6%) with that of RCC in common cases(57.4%). Conclusion:It is important for the young people to do health examination and B type ultrasound is a convenient and effective way for them. Radical nephrectomy would still be the main treatment modality and NSS could be an alternative in suitable young patients.

OBJECTIVE To investigate the diagnostic effect of cervical cytology test combined with high-risk human papilloma virus detection in the diagnotic and screening tests for cervical cancer and its precancerous lesion.METHODS To screen 590 women by conventional Papanicolaou smear test combined with oncogenic human papilloma virus DNA testing,selecting the abnormalities and high-risks in clinic,and using vaginoscopical biopsy or cervical canals scraping.We diagnosed patients according to histopathology.RESULTS From 590 patients there were no high risk changes when cytology and human papilloma virus(HPV) were negative;the rate of ≥cervical intraepithelial neoplasia(CIN) Ⅱ was 10.1% when cytology(-) and HPV(+);the rate of ≥CIN Ⅱ was 39.39% when cytology(+) and HPV(+);and the rate of ≥CIN Ⅱ was 12.3% when cytology(+) and HPV(-).There were no CIN Ⅲ or even worse.CONCLUSIONS The way of cytology combined with high risk HPV test is accurate,swift and sensitive,it can be used into screening cervical cancer and its precancerous lesion,futhermore,the screening interval can be lengthened if the results are negative.

Objective To explore the differences and infection factors of adherence of pulmonary rehabilitation (PR) in patients with different periods of chronic obstructive pulmonary disease (COPD). Methods The 304 patients were randomly distributed into two groups, stable COPD with PR group (n=178) and acute exacerbation COPD (AECOPD) with PR group (n=126). The course of PR was 12 weeks. According to the adherence definition (practical time of accompolish PR programm>70%of that planned time), patients were divided into good adherence group (n=115) and bad adherence group (n=189). The practical-perform time of PR, proportion of good adherence and PR quitting were compared between two groups. Basic data were collected and analyzed in two groups of patients. After the pulmonary rehabilitation, binary Logistic regression was used to analyse the compliance of pulmonary rehabilitation. Results The practical-perform time of PR and the proportion of good adherence were significantly higher in stable COPD with PR group than those of AECOPD with PR group (5 641.5±1 080.1 min vs. 4 426.5± 1 046.8 min,46.7%vs. 25.4%). The PR quitting proportion (10.6%, 21/199) was significantly lower in stable COPD with PR group than that of AECOPD with PR group (26.7%, 46/172). Compared with AECOPD PR group, the income level and 6 minutes walking distance (MWD) were significantly higher in stable COPD PR group, and the everage age, proportion of tabacco-exposed, MRC score and CAT score were significantly lower in stable COPD group (P<0.05). Logistic regression analysis showed that older-age, tabacco-exposed, higher MRC score and CAT score were risk factors for PR adherence, while the higher income level (≥3 000 yuan) and longer 6MWD were protective factors for PR time-adherence in patients with COPD (P<0.05). Conclusion The time adherence of COPD patients, who undergo pulmonary rehabilitation, is disappointing, especially post-exacerbation. Good adherence would be expected in younger age, non tabacco-exposed, higher income level and better health-related quality of life.

Objective To observe the effects of pulmonary rehabilitation (PR) on depression and anxiety at different stages of chronic obstructive pulmonary disease (COPD) patients. Methods Ninety-seven COPD patients hospitalized in Department of Respiratory Intensive Care Unit, Tianjin Chest Hospital, were randomly divided into two groups:control group (n=39) and acute exacerbation COPD (AECOPD) group (n=58). Patients in both groups were assessed for baseline date when symptoms were improved from acute phase (T1). Then patients in control group were received pharmaco-therapy and rehabilitation publicity without PR. When symptoms was relieved and stable (T2), patients were given 12-week PR (T3). The Beck depression inventory (BDI) scores and State-Trait Anxiety Inventory (STAI) scores were observed in both groups respectively. Results There were no statistics differences in baseline data in both groups (P>0.05). In control group, the BDI score was significantly lower at T3 than that of T1 and T2 (F=5.309, P<0.05). But there was no significant difference in BDI between T1 and T2 (P>0.05). Similarly in AECOPD group, the BDI score was significantly lower at T2 than that of T1 (t=3.612, P<0.05). At T1, there was no significant difference in BDI score between both groups (P>0.05). At T2 the BDI score was significantly lower in AECOPD group than that of control group, and also which was lower than that of T 3 of control group (P<0.05). In control group, the STAI score was significantly lower at T3 than that of T1 and T2 (F=9.852, P<0.05), but there was no significant difference in STAI between T1 and T2 (P>0.05). The STAI score was significantly lower in T2 than that of T1 in AECOPD group (t=5.091, P<0.05). There was no significant difference in STAI score at T1 between two groups (P>0.05). There was significantly lower STAI score at T2 in AECOPD group than that of control group (P<0.05),while there was no significant difference in STAI score at T3 between two groups (P>0.05). Conclusion Pulmonary rehabilitation at early stage may reduce the degree of depression and anxiety in AECOPD patients.