1.The Effects of Intensive Insulin Therapy on the ? Cell of Islets of Pancreas in Incipient Type Diabetes Patients
Journal of Medical Research 2006;0(08):-
0.05);The levels of basal FINS showed a significant differences between group A and group B(P0.05).Conclusions Intensive insulin treatment to newly diagnosed type 2 diabetes patients can control blood glucose rapidly to a desired level,and can recover the function of B cell better than traditional oral hypoglycemic agents by dissolving the virulence of glucose rapidly.
2.IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells
Journal of Chongqing Medical University 1986;0(02):-
Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria.
3.Reserch on expression of CXCR4 in non small cell lung cancer cells
Journal of Chongqing Medical University 1986;0(04):-
0.05).The positive expression rate of CXCR4 was 96% in cancer cells in regional metastasized lymph nodes.And its expression was lower than that in primary tumors(P
4.Experimental study of Renshen-heche Tablets on tonifying and warming Shen-Yang
Chinese Traditional Patent Medicine 1992;0(11):-
Objective: To study the effect of Resen heche tablets (RSHC) on tonyfing and warming Shen Yang. Methods: The mice model of Shen Yang deficiency was established by injecting hydrocortisone. The body weight, autonomic activity and swimming time in ice water in model mice were observed. The level of superoxide dismutase (SOD) in red cell and lipid peroxide (LPO) in plasma of aged rats were analyzed. The effect on anti stress in mice was examined. Results: RSHC had the remarkable effect to improve body weight and autonomic activity and to prolong swimming time in ice water in model mice, and to increase SOD activity and reduce LPO in blood of aged rats, and to elevate the ability of anti fatigue and anoxic tolerance in mice. Conclusion: RSHC drug has the effect on tonifying and warming Shen Yang; can promote growth, enhance organism activity, elevate the adaptation to circumstances and protect injury from free radicals.
5.Under local anesthesia hernia ring without tension hernia repair filling type surgical treatment the groin hernia
Chinese Journal of Postgraduates of Medicine 2012;(z1):31-32
Objective To evaluate the way of under local anesthesia hernia ring without tension hernia repair filling type surgical treatment the groin hernia surgery value of clinical application.Methods The clinical data of 256 cases were analyzed retrospectively for inguinal hernia in local anesthesia downside hernia ring filling type without tension hernia repair.Results All patients were recovered,under local anesthesia hernia ring without tension filling type hernia repair operation convenient operation,postoperative pain were light,the recurrence rate was low,less complications.Only 2 cases appeared the liquefaction of fat,1 case appeared urinary retention.Follow-up so far without recurrence.Conclusion Local anesthesia hernia ring without tension hernia method is safe and rehable,simple operation,and complex human anatomy,it is a kind of ideal choice to the ones who can't tolerate epidural anesthesia patients.
6.Effect of acute progressive normovolemic hemodilution with lactated Ringer's,gela-tin and hydroxyethyl starch on coagulation and survival rate in rabbits
Journal of Peking University(Health Sciences) 2004;0(03):-
0.05),the average survival time of HES solutions was longer than that after LR or GEL infusion(t=3.012,P
7.Effects of CpG ODN on dendritic cells and its mechanisms
Chinese Journal of Pathophysiology 2000;0(07):-
Oligodeoxynucleotide containing unmethylated cytosine phosphate-guanosine motif(CpG ODN) may induce high expression of CD80, CD86, CD83, HLA I and HLAⅡ molecules on dendritic cells(DC) and stimulate DC to produce high level of IL-6, IL-12, TNF-? and IFN-?. CpG ODN is demonstrated in vivo to be a very potent adjuvant for Th1 cells, regulating Th0 cells to develop toward Th1 cells. Its role for DC is characteristics of CpG ODN sequence specificity and species specificity. CpG ODN is, at present, considered as a pathogen associated molecular pattern which binds its specific receptor,Toll-like receptor 9,then functions through TLR/IL-1R signaling pathway. It may represent a new therapeutic drug for broad applications in infectious disease, autoimmune disease, allergy and cancer therapy.
8.Study on the reliability and validity of the Chinese version of the Nottingham Assessment of Functional Footcare
Chinese Journal of Practical Nursing 2015;31(6):450-453
Objective The study aimed to explore the reliability and validity of the Chinese version of the Nottingham Assessment of Functional Footcare (NAFF) in investigating the footcare behavior's level in patients with high risk of diabetic foot.Methods According to Bradley's principle,NAFF was converted into the Chinese version scale,then it was applied to 211 cases of patients with high risk of type 2 diabetic foot,and the reliability and validity of foot care be-havior scores were analyzed.Results In the Chinese version scale,the Cronbach's alpha coefficient was 0.77,test-retest reliability was 0.74.Content validity,ICVI was 0.80 to 1.00,S-CVI/Ave was 0.96,and the adjusted value of kappa was 0.75 to 1.00.Conclusions The questionnaire has been tested to have good reliability and validity.The NAFF possesses certain value in the clinical application and could be suitable for Chinese patients with high risk of type 2 diabetic foot.
9.Effect of ASODN transfection targeting VEGF-C gene on proliferation and apoptosis of Hela cells
Cancer Research and Clinic 2009;21(7):437-439,443
Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.
10.Expression of activator protein-1 in peripheral blood mononuclear cells (PBMCs) from patients with dermatomyositis and glucocorticoid effect on the expression
Chinese Journal of Dermatology 2011;44(12):889-890
Objective To investigate the expression of activator protein-1 (AP-1) in PBMCs from patients with dermatomyositis (DM) and glucocorticoid effect on the expression.Methods PBMCs were isolated from 15 normal human controls and 20 patients (including 12 patients with primary DM who had never received glucocorticoid treatment and 8 patients with recurrent DM who had stopped glucocorticoid treatment for more than 1 month),and classified into two parts:one was cultured in RPMI 1640 medium with dexamethasone of 80 μmol/L and 10% calf serum for 48 hours,and the other was frozen at -80 ℃ and reserved.Electrophoretic mobility shift assay (EMSA) was carried out to detect the activity of AP-1 in these cells.Results The expression (grayscale area) of AP-1 in the PBMCs from the normal controls was 4.93 ± 0.15 mm2.A significant decrease was induced by the treatment with dexamethasone in the expression (grayscale area) of AP-1 from PBMCs of patients with primary DM and those with recurrent DM (5.59 ± 0.39 vs.30.23 ± 0.49 mm2,5.69 ± 0.39 vs.34.79 ± 0.61 mm2,F =13812.64,P < 0.01 ).The activity of AP-1 was statistically higher in the PBMCs from patients with recurrent DM than in those from patients with primary DM before the treatment with dexamethasone (P < 0.01 ).Conclusions The enhanced activity of AP-1 may be an important factor inducing the inflammatory reaction in and recurrence of DM.Glucocorticoids may suppress the AP-1 activity to a certain degree.