ObjectiveTo determine the diurnal variation,moon phase,and seasonal variation in the onset of stroke. MethodsThe time parameters,including the time,the moon phase and the season of the stroke occurrence for 905 in-patients from 1989-2001 were investigated.ResultsThere was a significant difference (D=0.155,P<0.01) for circadian rhythm in the onset of 321 cases with cerebral infarction,and the incidence frequency at dawn was much higher than that at other time,but the difference of the moon phase and seasonal rhythm in the onset of cerebral infarction were not significant(P>0.05).There were not diurnal variation,moon phase and seasonal rhythms in the onset for cerebral haemorrhage(P>0.05).ConclusionsThe cerebral infarction occur more frequently at dawn time.

OBJECTIVE:To observe the clinical efficacy and safety of cinepazide maleate combined with oxiracetam in the treat-ment of vascular dementia. METHODS:62 patients with vascular dementia were randomly divided into treatment group and control group,with 31 patients in each group. Patients in control group were treated with Oxiracetam injection 4g/d for 3weeks. Patients in treat-ment group were treated with cinepazide maleate 160 mg/d for 3 weeks on the basis of control group. The patients of 2 groups were exam-ined with Mini-mental State Examination (MMSE)and Activity of Daily Living Scale (ADL)before and after treatment. RESULTS:There was statistically significant difference in MMSE and ADL scores of 2 groups before and after treatment(P<0.05);MMSE score and ADL score of treatment group was better than that of control group,with statistical significance(P<0.05);Obvious ADR was not found in both groups. CONCLUSIONS:Cinepazide maleate combined with oxiracetam can significantly improve ADL and MMSE score in patients with vascular dementia,improve the quality of life,showing significant and good clinical efficacy.

Tumor marker is a hot subject of research in oncology field,which would promote the diagnosis and treatment in clinic.Tumor markers of children's germ cell tumor include α-fetoprotein,glypican 3,SALL4,glial cell line-derived neurotropic factor receptorα-1(GFRα-1),Ki-67,octamer-binding transcription factor4,CD117,D2-40 and CD30.SALL4 would be used as a new tumor marker for germ cell tumor,specially for yolk sac tumor.GFRα-1 can be helpful for the diagnosis of immature teratoma.

112 cases with hemiplegia were randomly divided into three groups,training,acupuncture,and integrated training and acupuncture.Their effects were observed for three monthes.Results:the effect of the integrated training and acupuncture group was significantly better than the other two groups(P＜0.01).The results comfirmed that the motor function of the hemiplegic limbs was enhanced effectively with the integrated training and acupuncture.

ObjectiveTo study the effect of Xiangdan point injection in upper limb spasticity after stroke. Methods56 cases were divided into two groups: 28 cases for treatment group were treated with point injection Xiangdan, combined with routine therapy. Another 28 cases for control were only routinely therapy. ResultsThe cure rate of the treatment group was 29% ,the effective rates was 100%, while that of control group was 11% and 25% respectively (P<0.01).Conclusions Xiangdan point injection is more effective in upper limb spasticity after stroke than only routine therapy.

Objective To investigate the genetic characteristics of viruses associated with viral diarrhea in Jilin Province, in order to provide a scientific basis for making more effective prevention and control strategies of viral diarrhea.Methods A total of 211 fecal specimens from hospitalized children under 5 years of age with diarrhea and 7 sewage samples were collected in Jilin Province from January to July 2023. Initial screening was performed using qPCR, and positive specimens were further analyzed by RT-PCR to amplify target genes. Sequencing was conducted, and phylogenetic trees were constructed to analyze genetic relationships.Results In fecal specimens, the positive rates for rotavirus(RV), Norovirus(NoV),Sapovirus(SaV), astrovirus(AstV), and adenovirus(AdV) were 10. 9%, 48. 3%, 4. 3%, 1. 4%, and 5. 7%, respectively. In sewage samples, 5 AstV and 4 NoV were detected, along with 3 samples each of RV, SaV, and AdV. Sequencing and genotyping results revealed that RV G8P [8] was the predominant circulating strain. Among NoV strains, GⅡ.4 [P16] was the most prevalent, followed by GⅡ.4 [P31]. In fecal samples, GⅠ group strains included GⅠ.3 [P13] and GⅠ.1 [P1]. One SaV strain of GⅡ.5 was identified. For AstV, three strains of HAstV-1a and one strain of HAstV-5 were detected. Among AdV strains, three were identified as HAdV-41, three as HAdV-2, one as HAdV-1, and one as HAdV-5.Conclusion The pathogenic genes of viral diarrhea in Jilin Province are diverse, the dominant strains of RV have changed, and domestic sewage contains a variety of diarrhea viruses.

OBJECTIVE:To develop the data processing program of Digoxin blood drug concentration(DBDP)and to determine the range of effective blood concentration of Digoxin.METHODS:The DBDP program was developed by VISUAL BASIC6.0and run in Windows95system.The data of blood drug concentration,which was determined with flurescence polarization immunoassay(FPIA)in our hospital,was analyzed with DBDP program and effective blood drug concentration range of Digoxin was set up.RESULTS:The DBDP program integrated functions including pharmacokinetic parameter estimation,establishment of the effective blood drug concentration range,management of the data of the blood drug concentration moni?toring,automatic addition of patient's file,multifunctional fuzzy searching,retrospective analysis and clinical query about drug use.The effective blood drug concentration range of Digoxin was0.58～2.01ng/ml.CONCLUSION:The DBDP program is a very practical tool for the development of therapeutic drug monitoring and it is very necessary to develop blood drug concen?tration monitoring and establish the effective blood drug concentration range.

Objective:To screen for the differentially expressed genes in peripheral blood mononuclear cells(PBMCs) from type 2 diabetes patients with distal symmetric polyneuropathy(DSPN) by gene microarray.Methods: A 5 075 gene cDNA microarray was used to examine gene expression in PBMCs of 2 type 2 diabetes patients with DSPN(group 1),2 type 2 diabetes patients without DSPN(group 2),and 2 age-and sex-matched controls(control group).The differentially expressed genes were identified and subjected to real-time RT-PCR analysis.Results: Twenty-two genes were found to be differentially expressed,including cell metabolism and signal transduction genes,oncogenes and anti-oncogenes,DNA synthesis and repair genes,ion channel and transport protein genes,DNA-binding,transcription and transcription factor genes,and cytoskeleton genes.There were 4 genes up-regulated and 18 down-regulated.Real-time RT-PCR results of AK1 and FBXO7 were consistent with that of microarray analysis.Conclusion: Microarray expression profile of PBMC in type 2 diabetes patients with DSPN is differential.DSPN may be involved in cell metabolism,signal transduction,DNA synthesis,etc.

Objective To investigate the relationship of obesity with metabolic syndrome.Methods Six hundred and ninety-seven patients were chosen from the routine physical check-up from 2003 to 2005.These cases were divided into three groups according to body mass index(BMI).The detection rate of high triglyceride(TG) and low level of high-density lipoprotein cholesterol(HDL-C) were compared among groups of different BMI levels.The detection rate of hyperglycemia,hyperuricemia and high blood pressure among groups of different BMI levels was compared.Results With BMI increasing,the detection rate of high TG and low HDL-C also increased.The difference among three groups was statistically significant(P

OBJECTIVE:To optimize the preparation technology of cefixime suspension and study its stability.METHODS:The effects of hydroxypropyl cellulose,xanthan gum and sodium lauryl sulfate in different proportions on the sedimentation volume ratios of the suspensions were investigated by orthogonal experiments to optimize the preparation technology.The stability of the preparation was studied by accelerated test.RESULTS:The optimum preparation technology for cefixime suspension was as follows:the proportions of sodium dodecylsulfate,xanthan gum,and hydroxy-propyl methyl cellulose(HPMC) were 10%,20%,and 15%,respectively.The prepared suspension had simple formula and good stability,with all indexes up to the quality specification for suspension.CONCLUSION:The suspension prepared in accordance with this formula was able to meet the requirements of Chinese Pharmacopoeia on dry suspension.