Objective To investigate the distribution and the characteristics of drug resista-nce of coagulase negative Staphylococcus,and to provide the reference for properly choosi-ng antibiotic therapy of coagulase negative Staphylococcal infecs.Methods Contrasted 484 coagulase negative Staphylococcus(CNS) species isolated in hospital from 2001 to 2003 and 512 species from 2004 to 2007.Nosocomial CNS was identified and then drug resistance test was performed by K-B method,Nitrocefin method was utilized to detect β-lactamase.Clinical and Laboratory Standards Institute.Perfor-mance Standards forantimicro-bial susceptibility testing;Fifteenth information supplement.CLSI/NCCLS document M100-S17[J].Clinical and Laboratory Standards.Results The drug-resistant rate of.CNS to penicillin,erythromycin,oxacillin,amoxicillin/clavulanate,clindamycin,cepzolin,ceftriaxone and cefepime was high(>70%),whereas that to rifampin,nitrofurantoin,amikacin,tetracycline and trimetholvrim/sulfamethoxazole was low(<50%).No drug-resistant CNS strains to vancomyein were found.Conclusions,and no outstanding imparity from two groups.Conclusion The patterfi of resistance has changed greatly in the past 3 years.Clinical physicians should pay attention to properly choosing and using antibiotics.

Objective To evaluate the clinical value of thinprep cytologic test(TCT)and the Bethesda system(TBS)for the diagnosis of early cervical diseases.Methods A total of 635 patients in our hospital had been examined by TCT and TSB from December 2005 to 2006.the abnormal cases which had been selected by TCT were detected by colposcopical pathological examination and Hybrid Capture Ⅱ HPV testing.Results We found 66(10.23%)abnormal smears out of 635 cases,in which atypical squamous cell of undetermined significance(ASCUS)37 cases(5.83%),low grade squamous intraepithelial lesion(LSIL)18cases(2.80%),high grade squamous intraepithelial lesion(HSIL)10 cases(1.57%),quamous cellcarcinoma 1case(0.16%).The rates of coordinance with pathological results of colposcopy and HCⅡ were 92.95% and 87.00% respectively.Conclusions TCT and TBS,sometimes combined with the colposcspy and HPV DNA test,may be a reliable method to screen and diagnose the precancerosis of cervix.

Objective To compare the bond strength between three different treatment factors.Methods Sixty extracted human premolars were randomly divided into 3 groups(n=20).Edgewise bracket was bonded on each tooth.The 24 h shear bond strength and tensile bond strength in 3 groups were tested respectively.The adhesive remnant index was recorded.Results The significantly higher shear bond strengths were observed in the polishing group with tental politure and the group that bonded with light-cured fluoride glass ionomer cements after polishing(Group 1∶5.973?1.519,Group 2∶9.017?3.278,Group 3∶7.862?2.437,P

By means of literature investigation and analysis, the papers related to exercise-induced Achilles tendon rupture and operative restoration were summarized, which provide evidences for further analysis. Results demonstrated that exercise-induced Achilles tendon rupture was associated with anatomic structure and local affection of Achilles tendon, as well as athletic techniques. Operative therapy was recommended when the Achilles tendon was completely ruptured. With the development of tissue engineering tendon, more and more biological patch materials have been used in the clinical repair of Achilles tendon due to the superiority on the prevention of postoperative adhesion and the substitution of Achilles tendon.

Objective To study the evaluation function of Tei index for right heart function of elderly patients with chronic cor pulmonale .Methods Choosed 98 cases of elderly patients suspected chronic cor pulmonale and 68 cases of the medical health elder-ly population from March 2009 to March 2012 in our hospital as group A and B ,ultrasonic inspection and Tei index were measured , ultrasonic inspection indicators contains ,right room transverse diameter ,the main pulmonary artery diameter ,wall thickness and be-fore in pulmonary artery systolic pressure .Results A group of 31 patients(31 .6% ) with a clear cor pulmonale ultrasonic signs ,42 patients(42 .9% ) did not see clearly cor pulmonale ultrasonic signs ,but there was three tricuspid valve and (or) pulmonary valve re-gurgitation ,27 cases(25 .5% ) patients were not seen clear cor pulmonale ultrasonic signs ,and did not see the tricuspid valve and pulmonary valve regurgitation ,three types of Tei index patients were significantly higher than the group B (P<0 .05) .Conclusion Tei index can reflect patients right heart function and can be used as a sensitive index evaluation .

AIM: To optimize the preparation process of Fructus Schisandrae Chinensis dispensing granules METHODS:Schizandrin,schisantherin A,deoxyschizandrin,r-schizandrin were viewed as the indexes of judgement criteria,the influencing factors of extraction process as extract solvent,medical material particle diameter were studied and extraction parameters were optimized. RESULTS: Active components of dispensig granules can be extracted effectively by alcohol when medical materials were crushed.The best conditions were as follows:medical material were ground into 40 mesh,extracted two times by 70% alcohol,2 h per time. CONCLUSION: After being optimized,The process of dispensing granules is better than that of products on market.

Objective:To investigate the effect of GA on telomerase activity of breast cancer MDA-MB-435s cell.Methods:Human breast cancer cell line MDA-MB-435s was used in the study.Proliferation of MDA-MB-435s cells was measured with MTT assay.Cell cycle was analyzed by flow cytometry.The telomerase activity was measured by TRAP-ELISA assay.Results:GA inhibited the proliferation of MDA-MB-435s cells on a time-and dose-dependent manner.Aftertreatment of GA,MDA-MB-435s cells arrested at G2 /M phase.The telomerase activity of cells was decreasing compared with that of control cells(P

Objective To investigate the expression of CD44V6 and Bcl-2 in cervical carcinoma and clinical significant in the progress of th is type of cancer. Methods A total of 153 cases of cervical carcinoma, normal cervical epithelium and cervical dysplasia were investigated for the expression of CD44V6 and Bcl-2 with immunohistochemistry SP method. Results ① The positive rate of CD44V6 was 69.44% in cervical carcinoma, 45.45% in cervical dysplasia and 8.33% in normal cervix epithelium ( P 0.05 )and pathological grades( P 0.05 ).Conclusion The overexpression of CD44V6 and Bcl-2 may be l inked to the pathogenesis, development and metastasis of cervical carcinoma. Det ermination of CD44V6 and Bcl-2 may be useful for properly estimating the progno sis of cervical carcinoma.

Objective To explore the effect of IL-23-stimulated synovial fibroblasts (FLS) in rheumatoid arthritis (RA) on the formation of human osteoclast-like cell. Methods Fibroblasts from patients with RA and osteoarthritis (OA) were stimulated with various concentrations of IL-23 (1, 5 and 10 ng/ml). After incubation for 72 hours, the level of receptor activator of nuclear factor kappa B ligand (RANKL) mRNA were, measured by using real time PCR. Human monocytes (MN) were isolated and cocultured with IL-23-stimulated RA or OA FLS, and subsequently stained for tartrate-resistant acid phosphatase (TRAP) activity. Osteoclast-like cell molecule markers were measured using real time PCR. Results Various concentrations of IL-23 upregulated RANKL expression in rheumatoid FLS, but failed in OA FLS. In the system of IL-23-stimulated RA FLS cocuhured with human MN, TRAP-positive osteoclast-like cell was enhanced and high level expressions of molecule markers were induced. Whereas in the system of non IL-23-stimulated RA FLS or IL-23-stimulated OA FLS cocuhured with human MN there was no TRAP-positive osteoclast-like cell formation. Conclusion The results suggest that IL-23 induced osteoclast-like cell formation is via RANKL expressions in rheumatoid FLS.

Objective: To evaluate the effect of multiple amino acid substitutions of CⅡ263-272 peptide on collagen-induced arthritis,and explore a therapeutic strategy of rheumatoid arthritis.Methods: A panel of altered CⅡ263-272 peptides was synthesized with substitutions of TCR-contact residues of CⅡ263-272 with alanine.The competitive inhibition of APL to wild type CⅡ263-272 was analyzed by ~3H incorporation assay.CIA model was induced by intradermal injection of bovine CⅡ.Altered CⅡ peptide was injected subcutaneously in different doses(1,10,100 ?g,respectively,twice per week) after onset of CIA.The arthritis index,radiologic and histologic scores were recorded to evaluate the severity change of arthritis.Serum levels of IFN-? were examined by ELISA.Results: Competitive inhibition to wild type CⅡ263-272 of the altered CⅡ263-272 peptides(APL1,APL2,APL3) was found in PBMC from RA patients.Among them,APL3 with substitution of residues 267(Q),270(K) and 271(G) to A showed the most significant effect and thus was used in the treatment of CIA rats.We observed significantly reduced arthritis scores in CIA rats treated with 100 ?g/dose of APL as compared with rats treated with PBS and peptide control.The mean radiographic and histologic scores was also markedly lower in APL-treated CIA rats than in PBS or peptide control-treated rats.On day 35 after immunization,the serum level of IFN-? in rats was examined and a significantly low level of serum IFN-? was found in APL-treated rats.Conclusion: CⅡ263-272 peptide with TCR-contact residues substitutions inhibited joint destruction in CIA rats and down-regulated IFN-? production,suggesting that altered CⅡ peptide might be potentially therapeutic in rheumatoid arthritis.